- Open Access
How complete are current yeast and human protein-interaction networks?
© BioMed Central Ltd 2006
- Published: 1 December 2006
We estimate the full yeast protein-protein interaction network to contain 37,800-75,500 interactions and the human network 154,000-369,000, but owing to a high false-positive rate, current maps are roughly only 50% and 10% complete, respectively. Paradoxically, releasing raw, unfiltered assay data might help separate true from false interactions.
- Additional Data File
- Dead Reckoning
- Human Protein Interaction
- Assay Saturation
- Interactome Mapping
Networks are invaluable models for bettering our understanding of biological systems. Whether its constituent parts are molecules, cells, or living organisms, a network provides an organizing framework amenable to modeling the complex events that emerge from interactions among the parts. In functional genomics, concerted efforts over the past decade or so have produced rudimentary maps of the networks of genes, proteins, and metabolites controlling cells and, with these maps, have offered the promise of predictive, rather than just descriptive, models of molecular biology. Already, the network of physical interactions (the 'interactome') among yeast proteins, generated through a succession of experimental and algorithmic reconstructions, has proved its usefulness for discovering protein function [1, 2], predicting cellular behavior [3, 4], and the analysis of complex gene regulation [5–7]. Similar efforts for protein-interaction networks for Caenorhabditis elegans and Drosophila melanogaster are ongoing. We expect the human protein-interaction network to be equally informative; like the sequencing of the human genome, the construction of this map will represent a major step along the path towards understanding the functions of our genes.
Although maps of both the yeast and human protein-interaction networks are well under way, their completion poses many problems, not least because of the anticipated scale of the human network, which could require multiple testing of all possible pairs of around 20,000-25,000 human proteins - roughly 200 million to 300 million pairs. The scale of this effort raises many questions. How do we even measure completion? The network is, after all, unknown. How close are we to completing the networks? How do we assess errors in the maps? Would maps obtained using only a single technique suffice?
In this article, we discuss the techniques used up to now, describe strategies for recognizing network completion, and estimate our progress towards finished yeast and human protein-interaction maps. Even though large numbers of interactions have been mapped, we argue that assay false-positive rates are so high that only about half of the expected yeast network has been defined to date, and considerably less for the human one. Like whole-genome shotgun sequencing , interaction networks will require multiple-fold coverage for completion. We argue that raw interaction data should be released, pooled, and analyzed as a set, as was the case for the human genome sequence. Coverage is low enough and errors common enough in individual datasets to mean that the human interactome will only be fully mapped through integration of repeated analyses from many groups.
The primary approach to mapping human protein interactions is the same one that initiated the yeast interactome -the yeast two-hybrid assay [12, 13]. This classic assay involves the creation of two fusion proteins, the 'bait' protein fused to a DNA-binding domain and the 'prey' protein fused to a transcriptional activator domain. An interaction between bait and prey reconstitutes a complete transcription factor, detected by transcription of a reporter gene. This approach has already identified more than 5,000 interactions between human proteins [14, 15].
The second major approach is affinity purification followed by mass spectrometry [16, 17]. Here, epitope-tagged proteins are purified by affinity chromatography, and their co-purified interaction partners are identified by mass spectrometry. This assay excels at identifying in vivo protein complexes in yeast and other systems , particularly when used with tandem affinity purification (TAP)  and genomic knock-in of tags  rather than overexpression of transgenes. Most importantly, this technique bypasses exhaustive trials of all binary protein pairs and may scale up well to the size of the human interactome. On the downside, the assay may be biased toward abundant proteins . Also, human cells present more difficulties than yeast, especially in expressing tagged libraries of human genes and the need to grow large volumes of cells. Initial screens in human cells  have used transgenes, rather than genomic knock-ins, to simplify cloning.
The remaining main approaches to mapping yeast and human protein interactions are computational - inferring protein interactions by integrating evidence from comparative and functional genomics (see, for example [20, 22–25]). Although these are in silico rather than in vivo or in vitro interaction assays, they use experimental data such as DNA microarrays or genome sequences to infer protein interactions, and are, therefore, ultimately based on experimental observations . As large amounts of data are available, these data-mining methods scale-up easily and offer both in vivo relevance and the ability to detect stable and transient interactions. Disadvantages include the importance of measuring associated error rates and the need for independent validation to verify error rates.
Although the approaches described above are complementary, the differences between them have caused some confusion within the scientific community. The term 'protein-protein interaction' carries two meanings: direct physical binding or membership of the same multiprotein complex. The latter usage is common in the field at large: for example, both major efforts to map protein complexes in yeast describe "interactions" between co-complexed proteins [27, 28]. Part of the ambiguity in usage arises from the fact that few biochemical assays, apart from in vitro binding assays, truly distinguish the two cases. Currently, only yeast two-hybrid assays are regarded as measuring direct physical interaction between proteins and, at least in principle, even these interactions might occasionally be mediated through other members of a nuclear protein complex. Protein co-immunoprecipitation, often considered a definitive test of direct physical interactions, more typically measures co-complex interactions, much like the closely related affinity purification/mass spectrometry interaction assays. In addition, for the mass spectrometry interactions, one can consider the bait-prey interactions (the 'spoke' model ) as well as the prey-prey interactions (the 'matrix' model), with the latter typically of lower accuracy.
Computational and experimental approaches have now mapped a great many yeast and human protein interactions, but how many interactions should we expect? We argue here that the sizes of the complete yeast and human protein interaction networks will be larger than most early estimates. We do not yet know the size of any complete protein-interaction network. We can, however, roughly estimate the expected sizes for the yeast network using two different approaches that agree reasonably well. These estimates are derived from considering the interactions shared between each pair of large-scale protein interaction assays published so far.
To estimate the interactome size by intersection analysis, we first take the interactions in each dataset that are derived from the common sample space of the two assays. (Figure 3b shows only the interactions in this common sample space.) Each group purified around 2,000 TAP-tagged strains for mass spectrometry, with the common set of baits numbering 1,243, of which 1,128 yielded at least one identical interaction. While a true 'apples-to-apples' comparison of these results is difficult given the data that these two groups have published, as discussed by Goll and Uetz , we tried to extract the interactions derived from these common baits for this analysis from the published filtered datasets. After calculating error rates and subtracting false positives from the two datasets, their intersection was used to predict the number of interactions within the subspace they sample. That prediction was then scaled up to the size of the whole interactome (around 5,8002/2) to estimate the total number of protein-protein interactions in the organism.
Yeast protein-interaction assay false-positive rates: yeast datasets
Number of interactions
Derived false-positive rate* (%)
Published false-positive rate (%)
Average false-positive rate (%)
Uetz et al. 
32 †,47 , 50 , 51 
71 †, 78 , 85 , 91 
Gavin et al. 
14 †, 22 , <72 (upper bound )
Ho et al. 
83 , 81, 82, 80
55 †, <97 (upper bound )
Jansen et al. 
Gavin et al. 
78, 82, 86‡
Krogan et al. 
14,317 (7,123 core)
75, 79, 66‡ (59, 65, 37‡ core)
73 (54 core)
Prediction of the size of the yeast interactome
Estimated interactions in common search space
Projected interactome size (95% CI)
Gavin-Krogan (core) [27,28]
Ho-Krogan (core) [17,28]
These projected interactome sizes agree with those generated by a simple, very approximate, scaling argument: we observe approximately 5-10 unique interactions per yeast protein in current networks; multiplying these values by around 5,800 yeast genes gives estimates of approximately 29,000-58,000 interactions. These values are somewhat larger than previous estimates of 10,000-30,000 total yeast interactions [20, 29, 31, 37–39].
Human protein-interaction assay false-positive rates: human datasets
Number of unique interactions
Derived false-positive rates* (%)
Published false-positive rates (%)
Average false-positive rates (%)
Lehner and Fraser 
58,700 (9,396 core)
96, 94, 93 (86, 81, 69 core)
94 (79 core)
Rhodes et al. 
87, 86, 83
Stelzl et al. 
3,150 (902 core)
98, 98 (94,95 core)
98 (86 core)
Rual et al. 
8-66 †, 54 
Prediction of the size of the human interactome
Interactions in both datasets
Estimated interactions in common search space
Projected interactome size (95% CI)
Rual-Lehner (core) [14,40]
This analysis, with many others [20, 32, 37, 41–45], only reinforces the importance of measuring error rates when mapping protein interactions. Observing an interaction experimentally (for example, as in a yeast two-hybrid assay) does not guarantee a true positive interaction; that is, one that occurs in vivo under native conditions during the life of the organism. All assays, experimental and computational, show errors and should be accompanied by measures of confidence. Many published methods exist for estimating interaction assay error rates [20, 22, 37, 41–45] and for scoring individual protein-protein interactions. These latter scores either exploit assay-specific features  or use simple, but surprisingly effective, statistical criteria for separating true from false-positive interactions [43, 47, 48]. For example, although the full Ito et al.  yeast two-hybrid set has a measured false-positive rate of around 80%, a statistical measure based on the hypergeometric distribution can select a subset of around 45% of the interactions whose false-positive rate is only around 30% .
These high error rates underscore the difficulty in evaluating progress towards complete interactomes. Given these false-positive rates, and the resulting relatively small number of interactions detected in multiple assays, how far have we actually progressed towards the complete protein-interaction networks of yeast and humans?
As we can only approximate true interactome sizes, we have few sure measures of interactome completion beyond simply testing for coverage of confident interactions from the literature [2, 20]. However, two empirical methods, assay saturation and dead reckoning, suggest that we are far from finished with either the yeast or human interactomes.
Assay saturation captures the notion that, early in interaction-network mapping, each new interaction assay largely discovers novel interactions, as was observed for the first two large-scale yeast two-hybrid assays . Provided false-positive rates are well controlled, later assays should reveal proportionally fewer novel interactions, with the new interaction discovery rate dropping as interaction saturation approaches 100%. At this time, the portion of the interactome accessible to these assays will be complete, although this approach says nothing about how well this accessible portion covers the entire interactome. The saturation can be revealed by plotting, for each additional assay, the total interactions mapped versus the novel interactions mapped. Early assays fall along the diagonal (all interactions are new); later assays provide fewer new interactions, with the slope of the line decreasing, ultimately approaching zero for error-free, completely redundant assays.
The method of dead reckoning measures total interactome completion from the number of interactions assayed and their associated false-positive rates, just as sailors on the high seas estimated distances from the ship's speed and the time traveled. For this approach, we assume all interactions observed by more than one assay are true positives. When assays are uncorrelated, this assumption holds for about 99.9% of the time for both yeast and human, given our estimates of interactome size. The number of additional true positives contributed by an assay of size n is n(1 - fpr) - x, where x is the number of interactions already observed in previous assays. By this measure, the yeast experiments in Tables 1 and 2 plus the comprehensive literature databases have contributed 24,800 true-positive interactions, or around 50% of the estimated interactome. Of this total, nearly 18,000 interactions come from curated literature databases [2, 33], and 5,800 were detected in more than one high-throughput assay. Human protein-interaction assays have similarly covered about 25,000 true-positive interactions, or around 11% of the estimated interactome, with over 80% coming from sources based on literature mining. Note that these estimates assume that the literature sources are error-free, which is certainly not the case .
For both organisms, a number of factors could extend the current datasets to cover more of the interactome, such as considering the matrix model of interactions discovered by mass spectrometry . Although this increases the false positives, statistical scores can identify true positives , increasing the overall quality and number of interactions.
High error rates in large-scale assays dictate that the community must oversample the interactome in order to approach completion. Whole-proteome interactome mapping is, therefore, analogous to whole-genome shotgun sequencing : each assay reveals a subset of the interactions (sequence), requiring multiple-fold coverage of the interactome (genome) for completion of the true-positive set. In shotgun sequencing, assembly of sequencing reads is the algorithmically difficult step. By contrast, controlling and measuring error rates is currently the more challenging step in 'shotgun' interactome mapping. With false-positive rates exceeding 50%, and false-negative rates (the proportion of true interactions missed) for two-hybrid assays in particular approaching 90%, it is clear that each subspace must be sampled many times to provide complete coverage - and the problem remains of separating the true interactome from the false positives.
This last problem has made it clear that many alternative approaches will be required to complete the network. Comparing results from different approaches will continue to be crucial for validating interactions and estimating error rates, as the biases of one technique are easily overcome by integrating interactions from other methods. To this end, we strongly encourage all participants in interactome mapping to make public their raw data as well as their analyzed and filtered high-confidence interactions, as weak signals detected across multiple assays can be integrated to help distinguish real from spurious interactions. To further this discussion, many of the primary groups mapping the human protein interaction network met last August at the Joint Cold Spring Habor/Wellcome Trust Conference on Interactome Networks in Hinxton, UK, to compare results and coordinate efforts and announced plans to meet again next August. This effort may yet coalesce into a collaborative consortium like the human genome sequencing consortium, and an open forum now exists as the mapping proceeds.
Additional data on the statistics used are available online as Additional data file 1.
- Bandyopadhyay S, Sharan R, Ideker T: Systematic identification of functional orthologs based on protein network comparison. Genome Res. 2006, 16: 428-435. 10.1101/gr.4526006.PubMedPubMed CentralView ArticleGoogle Scholar
- Reguly T, Breitkreutz A, Boucher L, Breitkreutz BJ, Hon GC, Myers CL, Parsons A, Friesen H, Oughtred R, Tong A, et al: Comprehensive curation and analysis of global interaction networks in Saccharomyces cerevisiae. J Biol. 2006, 5: 11-10.1186/jbiol36.PubMedPubMed CentralView ArticleGoogle Scholar
- Almaas E, Kovacs B, Vicsek T, Oltvai ZN, Barabasi AL: Global organization of metabolic fluxes in the bacterium Escherichia coli. Nature. 2004, 427: 839-843. 10.1038/nature02289.PubMedView ArticleGoogle Scholar
- Herrgard MJ, Lee BS, Portnoy V, Palsson BO: Integrated analysis of regulatory and metabolic networks reveals novel regulatory mechanisms in Saccharomyces cerevisiae. Genome Res. 2006, 16: 627-635. 10.1101/gr.4083206.PubMedPubMed CentralView ArticleGoogle Scholar
- Kharchenko P, Church GM, Vitkup D: Expression dynamics of a cellular metabolic network. Mol Syst Biol. 2005, doi:10.1038/-msb4100023Google Scholar
- Maciag K, Altschuler SJ, Slack MD, Krogan NJ, Emili A, Greenblatt JF, Maniatis T, Wu LF: Systems-level analyses identify extensive coupling among gene expression machines. Mol Syst Biol. 2006, doi:10.1038/msb4100045Google Scholar
- Guido NJ, Wang X, Adalsteinsson D, McMillen D, Hasty J, Cantor CR, Elston TC, Collins JJ: A bottom-up approach to gene regulation. Nature. 2006, 439: 856-860. 10.1038/nature04473.PubMedView ArticleGoogle Scholar
- Jeong H, Mason SP, Barabasi AL, Oltvai ZN: Lethality and centrality in protein networks. Nature. 2001, 411: 41-42. 10.1038/35075138.PubMedView ArticleGoogle Scholar
- Gandhi TK, Zhong J, Mathivanan S, Karthick L, Chandrika KN, Mohan SS, Sharma S, Pinkert S, Nagaraju S, Periaswamy B, et al: Analysis of the human protein interactome and comparison with yeast, worm and fly interaction datasets. Nat Genet. 2006, 38: 285-293. 10.1038/ng1747.PubMedView ArticleGoogle Scholar
- Coulomb S, Bauer M, Bernard D, Marsolier-Kergoat MC: Gene essentiality and the topology of protein interaction networks. Proc Biol Sci. 2005, 272: 1721-1725. 10.1098/rspb.2005.3128.PubMedPubMed CentralView ArticleGoogle Scholar
- Weber JL, Myers EW: Human whole-genome shotgun sequencing. Genome Res. 1997, 7: 401-409.PubMedGoogle Scholar
- Fields S, Song O: A novel genetic system to detect protein-protein interactions. Nature. 1989, 340: 245-246. 10.1038/340245a0.PubMedView ArticleGoogle Scholar
- Golemis EA, Brent R: Fused protein domains inhibit DNA binding by LexA. Mol Cell Biol. 1992, 12: 3006-3014.PubMedPubMed CentralView ArticleGoogle Scholar
- Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, et al: Towards a proteome-scale map of the human protein-protein interaction network. Nature. 2005, 437: 1173-1178. 10.1038/nature04209.PubMedView ArticleGoogle Scholar
- Stelzl U, Worm U, Lalowski M, Haenig C, Brembeck FH, Goehler H, Stroedicke M, Zenkner M, Schoenherr A, Koeppen S, et al: A human protein-protein interaction network: a resource for annotating the proteome. Cell. 2005, 122: 957-968. 10.1016/j.cell.2005.08.029.PubMedView ArticleGoogle Scholar
- Gavin AC, Bosche M, Krause R, Grandi P, Marzioch M, Bauer A, Schultz J, Rick JM, Michon AM, Cruciat CM, et al: Functional organization of the yeast proteome by systematic analysis of protein complexes. Nature. 2002, 415: 141-147. 10.1038/415141a.PubMedView ArticleGoogle Scholar
- Ho Y, Gruhler A, Heilbut A, Bader GD, Moore L, Adams SL, Millar A, Taylor P, Bennett K, Boutilier K, et al: Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry. Nature. 2002, 415: 180-183. 10.1038/415180a.PubMedView ArticleGoogle Scholar
- Butland G, Peregrin-Alvarez JM, Li J, Yang W, Yang X, Canadien V, Starostine A, Richards D, Beattie B, Krogan N, et al: Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature. 2005, 433: 531-537. 10.1038/nature03239.PubMedView ArticleGoogle Scholar
- Rigaut G, Shevchenko A, Rutz B, Wilm M, Mann M, Seraphin B: A generic protein purification method for protein complex characterization and proteome exploration. Nat Biotechnol. 1999, 17: 1030-1032. 10.1038/13732.PubMedView ArticleGoogle Scholar
- von Mering C, Krause R, Snel B, Cornell M, Oliver SG, Fields S, Bork P: Comparative assessment of large-scale data sets of protein-protein interactions. Nature. 2002, 417: 399-403. 10.1038/nature750.PubMedView ArticleGoogle Scholar
- Bouwmeester T, Bauch A, Ruffner H, Angrand PO, Bergamini G, Croughton K, Cruciat C, Eberhard D, Gagneur J, Ghidelli S, et al: A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway. Nat Cell Biol. 2004, 6: 97-105. 10.1038/ncb1086.PubMedView ArticleGoogle Scholar
- Jansen R, Yu H, Greenbaum D, Kluger Y, Krogan NJ, Chung S, Emili A, Snyder M, Greenblatt JF, Gerstein M: A Bayesian networks approach for predicting protein-protein interactions from genomic data. Science. 2003, 302: 449-453. 10.1126/science.1087361.PubMedView ArticleGoogle Scholar
- Rhodes DR, Tomlins SA, Varambally S, Mahavisno V, Barrette T, Kalyana-Sundaram S, Ghosh D, Pandey A, Chinnaiyan AM: Probabilistic model of the human protein-protein interaction network. Nat Biotechnol. 2005, 23: 951-959. 10.1038/nbt1103.PubMedView ArticleGoogle Scholar
- Lee I, Date SV, Adai AT, Marcotte EM: A probabilistic functional network of yeast genes. Science. 2004, 306: 1555-1558. 10.1126/science.1099511.PubMedView ArticleGoogle Scholar
- Mellor JC, Yanai I, Clodfelter KH, Mintseris J, DeLisi C: Predictome: a database of putative functional links between proteins. Nucleic Acids Res. 2002, 30: 306-309. 10.1093/nar/30.1.306.PubMedPubMed CentralView ArticleGoogle Scholar
- Fraser AG, Marcotte EM: A probabilistic view of gene function. Nat Genet. 2004, 36: 559-564. 10.1038/ng1370.PubMedView ArticleGoogle Scholar
- Gavin AC, Aloy P, Grandi P, Krause R, Boesche M, Marzioch M, Rau C, Jensen LJ, Bastuck S, Dumpelfeld B, et al: Proteome survey reveals modularity of the yeast cell machinery. Nature. 2006, 440: 631-636. 10.1038/nature04532.PubMedView ArticleGoogle Scholar
- Krogan NJ, Cagney G, Yu H, Zhong G, Guo X, Ignatchenko A, Li J, Pu S, Datta N, Tikuisis AP, et al: Global landscape of protein complexes in the yeast Saccharomyces cerevisiae. Nature. 2006, 440: 637-643. 10.1038/nature04670.PubMedView ArticleGoogle Scholar
- Bader GD, Hogue CW: Analyzing yeast protein-protein interaction data obtained from different sources. Nat Biotechnol. 2002, 20: 991-997. 10.1038/nbt1002-991.PubMedView ArticleGoogle Scholar
- Seber GAF: The Estimation of Animal Abundance and Related Parameters. 1982, Caldwell, NJ: Blackburn Press, 2Google Scholar
- Grigoriev A: On the number of protein-protein interactions in the yeast proteome. Nucleic Acids Res. 2003, 31: 4157-4161. 10.1093/nar/gkg466.PubMedPubMed CentralView ArticleGoogle Scholar
- D'haeseleer P, Church GM: Estimating and improving protein interaction error rates. Proc IEEE Comput Syst Bioinform Conf: August 16-19 2004; California. 2004, IEEE Computer Society, 216-223.Google Scholar
- Guldener U, Munsterkotter M, Oesterheld M, Pagel P, Ruepp A, Mewes HW, Stumpflen V: MPact: the MIPS protein interaction resource on yeast. Nucleic Acids Res. 2006, D436-D441. 10.1093/nar/gkj003. 34 DatabaseGoogle Scholar
- Goll J, Uetz P: The elusive yeast interactome. Genome Biol. 2006, 7: 223-PubMedPubMed CentralView ArticleGoogle Scholar
- Uetz P, Giot L, Cagney G, Mansfield TA, Judson RS, Knight JR, Lockshon D, Narayan V, Srinivasan M, Pochart P, et al: A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae. Nature. 2000, 403: 623-627. 10.1038/35001009.PubMedView ArticleGoogle Scholar
- Ito T, Chiba T, Ozawa R, Yoshida M, Hattori M, Sakaki Y: A comprehensive two-hybrid analysis to explore the yeast protein interactome. Proc Natl Acad Sci USA. 2001, 98: 4569-4574. 10.1073/pnas.061034498.PubMedPubMed CentralView ArticleGoogle Scholar
- Sprinzak E, Sattath S, Margalit H: How reliable are experimental protein-protein interaction data?. J Mol Biol. 2003, 327: 919-923. 10.1016/S0022-2836(03)00239-0.PubMedView ArticleGoogle Scholar
- Tucker CL, Gera JF, Uetz P: Towards an understanding of complex protein networks. Trends Cell Biol. 2001, 11: 102-106. 10.1016/S0962-8924(00)01902-4.PubMedView ArticleGoogle Scholar
- Legrain P, Wojcik J, Gauthier JM: Protein-protein interaction maps: a lead towards cellular functions. Trends Genet. 2001, 17: 346-352. 10.1016/S0168-9525(01)02323-X.PubMedView ArticleGoogle Scholar
- Lehner B, Fraser AG: A first-draft human protein-interaction map. Genome Biol. 2004, 5: R63-10.1186/gb-2004-5-9-r63.PubMedPubMed CentralView ArticleGoogle Scholar
- Bader JS, Chaudhuri A, Rothberg JM, Chant J: Gaining confidence in high-throughput protein interaction networks. Nat Biotechnol. 2004, 22: 78-85. 10.1038/nbt924.PubMedView ArticleGoogle Scholar
- Deane CM, Salwinski L, Xenarios I, Eisenberg D: Protein interactions: two methods for assessment of the reliability of high throughput observations. Mol Cell Proteomics. 2002, 1: 349-356. 10.1074/mcp.M100037-MCP200.PubMedView ArticleGoogle Scholar
- Lee I, Narayanaswamy R, Marcotte EM: Bioinformatic prediction of yeast gene function. Yeast Gene Analysis. Edited by: Stansfield I, Stark M. 2007, Amsterdam: Elsevier Press, Preprint at http://polaris.icmb.utexas.edu/publications.htmlGoogle Scholar
- Mrowka R, Patzak A, Herzel H: Is there a bias in proteome research?. Genome Res. 2001, 11: 1971-1973. 10.1101/gr.206701.PubMedView ArticleGoogle Scholar
- Patil A, Nakamura H: Filtering high-throughput protein-protein interaction data using a combination of genomic features. BMC Bioinformatics. 2005, 6: 100-10.1186/1471-2105-6-100.PubMedPubMed CentralView ArticleGoogle Scholar
- Giot L, Bader JS, Brouwer C, Chaudhuri A, Kuang B, Li Y, Hao YL, Ooi CE, Godwin B, Vitols E, et al: A protein interaction map of Drosophila melanogaster. Science. 2003, 302: 1727-1736. 10.1126/science.1090289.PubMedView ArticleGoogle Scholar
- Samanta MP, Liang S: Predicting protein functions from redundancies in large-scale protein interaction networks. Proc Natl Acad Sci USA. 2003, 100: 12579-12583. 10.1073/pnas.2132527100.PubMedPubMed CentralView ArticleGoogle Scholar
- Schlitt T, Palin K, Rung J, Dietmann S, Lappe M, Ukkonen E, Brazma A: From gene networks to gene function. Genome Res. 2003, 13: 2568-2576. 10.1101/gr.1111403.PubMedPubMed CentralView ArticleGoogle Scholar
- Ramani AK, Bunescu RC, Mooney RJ, Marcotte EM: Consolidating the set of known human protein-protein interactions in preparation for large-scale mapping of the human interactome. Genome Biol. 2005, 6: R40-10.1186/gb-2005-6-5-r40.PubMedPubMed CentralView ArticleGoogle Scholar
- Eppig JT, Bult CJ, Kadin JA, Richardson JE, Blake JA, Anagnostopoulos A, Baldarelli RM, Baya M, Beal JS, Bello SM, et al: The Mouse Genome Database (MGD): from genes to mice-a community resource for mouse biology. Nucleic Acids Res. 2005, D471-D475. 33 DatabaseGoogle Scholar
- Harborth J, Elbashir SM, Bechert K, Tuschl T, Weber K: Identification of essential genes in cultured mammalian cells using small interfering RNAs. J Cell Sci. 2001, 114: 4557-4565.PubMedGoogle Scholar
- Kittler R, Putz G, Pelletier L, Poser I, Heninger AK, Drechsel D, Fischer S, Konstantinova I, Habermann B, Grabner H, et al: An endoribonuclease-prepared siRNA screen in human cells identifies genes essential for cell division. Nature. 2004, 432: 1036-1040. 10.1038/nature03159.PubMedView ArticleGoogle Scholar
- Amsterdam A, Nissen RM, Sun Z, Swindell EC, Farrington S, Hopkins N: Identification of 315 genes essential for early zebrafish development. Proc Natl Acad Sci USA. 2004, 101: 12792-12797. 10.1073/pnas.0403929101.PubMedPubMed CentralView ArticleGoogle Scholar
- Mishra GR, Suresh M, Kumaran K, Kannabiran N, Suresh S, Bala P, Shivakumar K, Anuradha N, Reddy R, Raghavan TM, et al: Human protein reference database - 2006 update. Nucleic Acids Res. 2006, D411-D414. 10.1093/nar/gkj141. 34 DatabaseGoogle Scholar
- Alfarano C, Andrade CE, Anthony K, Bahroos N, Bajec M, Bantoft K, Betel D, Bobechko B, Boutilier K, Burgess E, et al: The Biomolecular Interaction Network Database and related tools 2005 update. Nucleic Acids Res. 2005, D418-D424. 33 DatabaseGoogle Scholar
- Joshi-Tope G, Gillespie M, Vastrik I, D'Eustachio P, Schmidt E, de Bono B, Jassal B, Gopinath GR, Wu GR, Matthews L, et al: Reactome: a knowledgebase of biological pathways. Nucleic Acids Res. 2005, D428-D432. 33 DatabaseGoogle Scholar