Volume 8 Supplement 1

Transposons in vertebrate functional genomics


Edited by Stephen C Ekker and Zoltán Ivics

  1. Review

    Retroviral enhancer detection insertions in zebrafish combined with comparative genomics reveal genomic regulatory blocks - a fundamental feature of vertebrate genomes

    A large-scale enhancer detection screen was performed in the zebrafish using a retroviral vector carrying a basal promoter and a fluorescent protein reporter cassette. Analysis of insertional hotspots uncovere...

    Hiroshi Kikuta, David Fredman, Silke Rinkwitz, Boris Lenhard and Thomas S Becker

    Genome Biology 2007 8(Suppl 1):S4

    Published on: 31 October 2007

  2. Review

    Transposable elements and the dynamic somatic genome

    Although alterations in the genomes of somatic cells cannot be passed on to future generations, they can have beneficial or detrimental effects on the host organism, depending on the context in which they occu...

    Lara S Collier and David A Largaespada

    Genome Biology 2007 8(Suppl 1):S5

    Published on: 31 October 2007

  3. Review

    Tol2: a versatile gene transfer vector in vertebrates

    The medaka fish Tol2 element is an autonomous transposon that encodes a fully functional transposase. The transposase protein can catalyze transposition of a transposon construct that has 200 and 150 base pairs o...

    Koichi Kawakami

    Genome Biology 2007 8(Suppl 1):S7

    Published on: 31 October 2007

  4. Review

    Transposons as tools for enhancer trap screens in vertebrates

    DNA transposons are efficient tools in transgenesis and have therefore become popular in the analysis of the regulatory genome in vertebrates via enhancer trap screens. Here, I discuss recent progress in this ...

    Vladimir Korzh

    Genome Biology 2007 8(Suppl 1):S8

    Published on: 31 October 2007

  5. Review

    Insertional mutagenesis strategies in zebrafish

    We review here some recent developments in the field of insertional mutagenesis in zebrafish. We highlight the advantages and limitations of the rich body of retroviral methodologies, and we focus on the mecha...

    Sridhar Sivasubbu, Darius Balciunas, Adam Amsterdam and Stephen C Ekker

    Genome Biology 2007 8(Suppl 1):S9

    Published on: 31 October 2007

  6. Review

    Meganuclease and transposon mediated transgenesis in medaka

    From among a plethora of various gene delivery methods, the researcher must choose the right one according to availability for a given species and the precise application the transgenic animal is intended for....

    Clemens Grabher and Joachim Wittbrodt

    Genome Biology 2007 8(Suppl 1):S10

    Published on: 31 October 2007

  7. Review

    Manipulating the Xenopusgenome with transposable elements

    The study of amphibian embryogenesis has provided important insight into the mechanisms of vertebrate development. The frog Xenopus laevis has been an important model of vertebrate cell biology and development fo...

    Donald A Yergeau and Paul E Mead

    Genome Biology 2007 8(Suppl 1):S11

    Published on: 31 October 2007

  8. Review

    Pigs taking wing with transposons and recombinases

    Swine production has been an important part of our lives since the late Mesolithic or early Neolithic periods, and ranks number one in world meat production. Pig production also contributes to high-value-added...

    Karl J Clark, Daniel F Carlson and Scott C Fahrenkrug

    Genome Biology 2007 8(Suppl 1):S13

    Published on: 31 October 2007

  9. Review

    Transposons for cancer gene discovery: Sleeping Beautyand beyond

    The use of Sleeping Beauty transposons as somatic mutagens to discover cancer genes in hematopoietic tumors and sarcomas has been documented. Here, we discuss the future of Sleeping Beauty for cancer genetic stud...

    Lara S Collier and David A Largaespada

    Genome Biology 2007 8(Suppl 1):S15

    Published on: 31 October 2007

  10. Review

    Mutagenesis in rodents using the L1 retrotransposon

    LINE1 (L1) retrotransposons are genetic elements that are present in all mammalian genomes. L1s are active in both humans and mice, and are capable of copying themselves and inserting the copy into a new genom...

    Eric M Ostertag, Blair B Madison and Hiroki Kano

    Genome Biology 2007 8(Suppl 1):S16

    Published on: 31 October 2007