- Open Access
Integrative epigenomic analysis reveals unique epigenetic signatures involved in unipotency of mouse female germline stem cells
Genome Biology volume 17, Article number: 162 (2016)
Germline stem cells play an essential role in establishing the fertility of an organism. Although extensively characterized, the regulatory mechanisms that govern the fundamental properties of mammalian female germline stem cells remain poorly understood.
We generate genome-wide profiles of the histone modifications H3K4me1, H3K27ac, H3K4me3, and H3K27me3, DNA methylation, and RNA polymerase II occupancy and perform transcriptome analysis in mouse female germline stem cells. Comparison of enhancer regions between embryonic stem cells and female germline stem cells identifies the lineage-specific enhancers involved in germline stem cell features. Additionally, our results indicate that DNA methylation primarily contributes to female germline stem cell unipotency by suppressing the somatic program and is potentially involved in maintenance of sexual identity when compared with male germline stem cells. Moreover, we demonstrate down-regulation of Prmt5 triggers differentiation and thus uncover a role for Prmt5 in maintaining the undifferentiated status of female germline stem cells.
The genome-wide epigenetic signatures and the transcription regulators identified here provide an invaluable resource for understanding the fundamental features of mouse female germline stem cells.
Germline stem cells (GSCs) are of essential importance for genome transmission from generation to generation . Although unipotent, GSCs have a unique capability to continuously generate gametes. In recent years, extensive efforts have been made to understand the specification of primordial germ cells (PGCs) and the profound epigenetic reprogramming (including genome-wide DNA demethylation and histone remodeling) which is necessary for the zygote to acquire totipotency after fertilization [2, 3]. Much less is known about the regulatory mechanisms that govern the fundamental properties of mammalian female GSCs. It has long been believed that female mammals lose the ability to produce oocytes at birth [4–8]. However, this concept has been reshaped by recent studies in which female germline stem cells (FGSCs) have been identified in postnatal ovaries of various mammalian organisms [9–13]. Therefore, more attention needs to be paid to characterizing the mechanisms for the maintenance of unipotency and undifferentiated status of FGSCs, which is critical for understanding GSC biology.
Knowledge on the close coordination of genetic and epigenetic regulation is important for understanding the basic properties of stem cells and their differentiation . In addition to genetic programs directed by lineage-specific transcription factors, genome-wide epigenetic modifications are also actively involved in development and cell fate determination, which constitute another layer of regulation beyond the genome sequence. Epigenetic profiling has been well documented in studies of embryonic stem cells (ESCs) . For example, some promoter regions in ESCs are co-marked by H3K4me3 and H3K27me3 and have been termed bivalent domains. These bivalent genes are poised at the ESC stage and could be activated in downstream development stages . Subsequently, our genome-wide profiling analysis of H3K4me3 and H3K27me3 revealed that the different combinations of histone modifications modulate diverse transcriptional patterns and are involved in the fundamental properties of ESCs . A study of H3K4me1 and H3K27ac, histone modifications marking enhancers, indicated that they are cell type-specific and involved in determining cellular identity . Identifying and characterizing regulatory DNA elements (e.g., promoters and enhancers) is hugely difficult due to the lack of recognizable and consistent sequence features but epigenetic profiling in ESCs has proven that it is a powerful tool to delineate these. Meanwhile, these profiling analyses provide insights into the understanding of stem cell biology.
In previous reports, we generated mouse FGSC lines and demonstrated that FGSCs could undergo oogenesis once transplanted into ovaries of infertile mice and give rise to offspring [9, 13]. Although this study was viewed to be useful for both basic research and medicine , the regulatory mechanisms that govern the identity of FGSCs remain elusive. Here, we carried out extensive epigenomic profiling and RNA sequencing (RNA-Seq) analyses with the aim of understanding epigenetic and genetic control in mouse FGSCs.
FGSCs exhibit lineage-specific gene expression signatures
We first characterized the cultured FGSCs by examining the molecular signatures associated with germline development. Immunocytochemical analysis indicated that FGSCs are positive for germline-specific markers Mvh and Fragilis (Additional file 1: Figure S1a). Then we examined the expression of other germ cell-specific markers by reverse transcription polymerase chain reaction (RT-PCR). We found that Dazl and Stella are expressed in FGSCs (Additional file 1: Figure S1b). The characteristics detected in this study are consistent with the observations we reported previously [9, 13].
To obtain a global view of the transcription pattern of FGSCs, we performed transcriptional profiling of mRNA with strand-specific RNA-Seq (Fig. 1a) and compared our data with those from ESCs . In contrast to Nanog and Sox2, which are specifically expressed in ESCs, we found that Ifitm3/Fragilis, Ptx3, and GM1673 are selectively expressed in FGSCs (Fig. 1b). Moreover, we found that Akt1 is highly expressed in FGSCs; the Akt1 pathway is involved in self-renewal of mouse germline stem cells . Piwi proteins bind piwi-interacting RNA (piRNA), which is responsible for repetitive element silencing during germline development. Intriguingly, we observed that the piwi proteins Mili, Miwi, and Miwi2 are not actively expressed, probably due to the Piwi–piRNA pathway, which is particularly involved in gametogenesis . Thus, these observations verify the known molecular signatures of FGSCs.
Extensive mapping of chromatin marks in FGSCs
To explore the chromatin state and its effect on the properties of FGSCs, we performed chromatin immunoprecipitation sequencing (ChIP-Seq) to generate genome-wide maps by profiling four histone modifications (H3K4me1, H3K27ac, H3K4me3, and H3K27me3) and RNA polymerase II (RNA Pol II) occupancy. We also profiled global DNA methylation by MethylCap-Seq. In addition, we measured gene expression levels with RNA-Seq and generated more than 108 million uniquely mapped reads for detecting gene expression in FGSCs (Fig. 1a; Additional file 1: Table S1). For each high-throughput sequencing analysis, at least two biological replicates were performed, which are fairly correlated (Additional file 1: Figure S2a). All data sets have been deposited in a public database.
The sequencing data were visualized in the Integrative Genomics Viewer (IGV) by generating histograms of normalized densities of ChIP fragments across the FGSC genome (Fig. 1c; Additional file 1: Figure S3) . The map of histone modifications and DNA methylation shows signal distributions that are consistent with their functions . For example, H3K4me3 has been regarded as a hallmark of transcription initiation and is primarily localized at promoters . In our study we found 90 % of H3K4me3 sites are located at promoter regions. A case in point is the presence of strong H3K4me3 at the promoter of Ifitm3/Fragilis, which encodes a protein used to generate the FGSC line [9, 13]. H3K27ac and RNA Pol II are associated with active transcription and we found they are highly enriched at promoter regions in FGSCs (Additional file 1: Figure S2b). Additionally, we observed that H3K27me3 is broadly distributed across the FGSC genome, similar to what we found in ESCs . Taken together, these observations suggest the data sets we generated here are able to be used to identify the cis-regulatory elements in FGSCs.
Active enhancers distinguish FGSCs from ESCs
It has been recognized that enhancer regions are marked by H3K4me1 in a cell type-specific manner and involved in determining cellular identity [17, 24]. Moreover, these cis-regulatory elements could be further classified into “active” or “poised” enhancers based on the presence of H3K27ac . Consistent with these observations, we found both types of enhancer sites in FGSCs (Fig. 2a). Examination of the genomic distribution of both types of enhancers relative to transcription start sites (TSSs) indicated that these enhancers exhibit a similar distribution pattern, with the majority located away from TSSs (Fig. 2b).
Both ESCs and FGSCs are capable of self-renewal in vitro, whereas they possess different developmental potential. To examine the underlying regulatory elements exclusively involved in FGSCs, we performed a K-means clustering analysis with active enhancer sites co-modified by H3K4me1 and H3K27ac and generated four major classes (Fig. 2c). Not surprisingly, genes associated with the FGSC-specific active enhancer regions (single nearest genes within 200 kb) exhibit higher transcriptional activity in FGSCs compared with ESCs (Fig. 2d). To understand how the lineage-specific enhancers contribute to FGSC identity, we performed gene ontology (GO) analysis with the Genomic Regions Enrichment of Annotations Tool (GREAT) . We found the FGSC-specific enhancer peaks (class 2) are highly enriched for genes involved in reproduction-related phenotypes (Fig. 2e; Additional file 2: Table S2), including reproductive system physiology (e.g., Notch2, Npr2, and Nr2f2) and female fertility (e.g., Ptgs2, Ptx3, and vrk1) (Additional file 1: Figure S4a). Meanwhile, we found ESC-specific active enhancers (Fig. 2c, class 3) are mainly involved in embryogenesis and the active enhancers shared by ESCs and FGSCs are enriched for mitotic cell cycle-related genes (Additional file 1: Figure S4b).
A bivalent domain chromatin signature is not widespread in FGSCs
Bivalent domain chromatin has been reported to be involved in developmental plasticity of ESCs . Our previous study further revealed that the bivalent promoters are prevalent across the ESC genome . In this study, we examined the presence of bivalent promoters in FGSCs. To our surprise, we observed that bivalent promoters in FGSCs are much less prevalent (Fig. 2f; Additional file 3: Table S3). It is less likely that this observation results from inefficient H3K27me3 ChIP-Seq as a considerable number of H3K27me3-marked regions are identified in FGSCs (Additional file 3: Table S3). A similar phenomenon was reported in multipotent neural crest cells . These observations suggest that an “epigenetic code” other than the bivalent domain is responsible for the developmental plasticity of stem/progenitor cells.
DNA methylation contributes to FGSC identity by suppressing the somatic program
One of the major issues in germline stem cell biology is how unipotency is maintained. During the specification of germ cells, the Blimp1/Prmt5 complex plays an important role in the maintenance of unipotency through repressing targets by generation of repressive H2A/H4R3me2s and this complex translocates from the nucleus to cytoplasm in embryonic day (E)11.5 PGCs . Moreover, we found that Prdm1, the gene encoding Blimp1, is not actively expressed in FGSCs. To understand how the unipotency of FGSCs is maintained, we examined the presence of DNA methylation, another epigenetic mark critically involved in gene silencing, across the FGSC genome. To this end, we generated a DNA methylation profile by MethylCap-Seq and compared our data with the data sets of the precursors of FGSCs generated by MeDIP-seq. We observed a remarkable difference in global DNA methylation patterns among ESCs, E11.5 PGCs, and FGSCs; the correlation between FGSCs and E11.5 PGCs is 0.05 and between FGSCs and ESCs is 0.27 (Fig. 3a, b).
To understand the significance of DNA methylation in FGSCs, we performed a clustering analysis with the data sets of ESCs, E11.5 PGCs, and FGSCs; the FGSC-specific methylated promoter regions (Fig. 3c, class 1) were used for GREAT analysis . Notably, functional annotation revealed that the FGSC-specific methylated genes are mainly involved in somatic developmental processes (Fig. 3d), including Hox, Fox, and Tbx family transcription factors. Although viewed as a silencing epigenetic mark, growing evidence has revealed that the effect of DNA methylation on transcription is dependent on the genomic context . We categorized the methylated regions of the FGSC genome into three groups and examined the relationship of DNA methylation and transcription activity. Similar to the previous study , we found that the transcription levels of genes with methylated promoters are lower than those of genes in the other two categories (Fig. 3e). Surprisingly, we observed that more than 90 % of genes with a methylated promoter are simultaneously methylated at the gene body (Additional file 4: Table S4), an epigenetic signature associated with active transcription . To further explore the contribution of DNA methylation in FGSCs, we knocked down Dnmt1 and found that several somatic development-related genes with DNA methylation and low occupancy of RNA Pol II at promoter regions were remarkably up-regulated (Fig. 3f). These results suggest that DNA methylation critically contributes to FGSC identity.
Differential DNA methylation is involved in sexual identity maintenance of FGSCs
Germ cells are sexually bipotential in the early embryonic gonad and commit to either male or female development by E13.5 . Although it is generally recognized that sex determination of germ cells is primarily determined by signaling molecules from the soma, increasing evidence has suggested the “sex” of the soma and germ cells must match each other for proper gametogenesis . Nevertheless, it remains unclear how FGSCs intrinsically match the soma to maintain sexual identity; we thus asked whether DNA methylation is involved in this process.
To address this issue, we compared the DNA methylation pattern in FGSCs with that in male germline stem cells (MGSCs) measured by bisulfite sequencing  (Fig. 4a). We analyzed the DNA methylation datasets with the method reported previously  and found the Pearson correlation to be 0.229 (Fig. 4b), suggesting a low correlation of DNA methylation between male and female GSCs. We particularly compared the methylated promoter regions. Among the methylated promoter regions of 11,936 genes, only 2689 (22.5 %) exhibit a similar DNA methylation level, whereas the majority exhibit a gender-specific methylation pattern (Fig. 4c; Additional file 5: Table S5). GO analysis of these female-specific methylated genes indicated they are involved in terms related to male sexual development (Fig. 4d). For example, Bcl2l11, Nr0b1, and Sfrp2 play a critical role in development of male characteristics in mouse [35–37]. Here, we observed the promoters of these three genes are exclusively methylated in FGSCs and hypomethylated in MGSCs (Additional file 1: Figure S5). Transposable elements constitute 37 % of the mouse genome  and we investigated the DNA methylation of six categories of transposable elements that overlap with CpG islands. In contrast to the higher DNA methylation frequency of long interspersed nuclear element (LINE) L1, long terminal repeat ERV1, and intracisternal A-type particles (IAPs) in MGSCs, DNA transposons, SINE B1, and SINE B2 are more frequently methylated in FGSCs (Fig. 4e). We also investigated DNA methylation at imprinted loci. We observed the sex-specific DNA methylation pattern at differentially methylated regions (DMRs) of the imprinted loci examined (Fig. 4f; Additional file 1: Figure S6). Together, these results suggest DNA methylation is involved in sexual identity maintenance of FGSCs.
Prmt5 is implicated in FGSC biology
As mentioned above, Prmt5 forms a complex with the PGC determinant Blimp1 and is involved in the commitment of germ cell lineage, whereas the Blimp1/Prmt5 complex translocates from the nucleus to cytoplasm after E11.5 . Our RNA-Seq data show Prmt5 is actively expressed in FGSCs. Therefore, it remains intriguing to explore its function in FGSCs. Given the subcellular localization dynamics of Prmt5 during the early germline development, we first examined its localization and found that Prmt5 is primarily localized in the cytoplasm of FGSCs (Fig. 5a). We then performed a Prmt5 knockdown assay (Additional file 1: Figure S7) and examined the biological consequences. We found that the meiosis-related genes (including Figla, Sycp3, and Sycp1) and oogenesis-related genes (including Zp2 and Zp3) are up-regulated upon Prmt5 knockdown (Fig. 5b), suggesting that Prmt5 is involved in maintenance of the undifferentiated status of FGSCs.
To gain a global view of the effect of Prmt5 on gene expression, we performed RNA-Seq analysis using Prmt5 knockdown FGSCs and examined the Prmt5-responsive genes. Compared with the control, 2916 genes were found to be statistically up-regulated upon Prmt5 knockdown (p < 0.05; Fig. 5c; Additional file 6: Table S6). Using DAVID , we performed GO analysis and found that, in addition to meiosis-related GO terms, some development-related biological processes (including heart development, embryonic development ending in birth or egg hatching, in utero embryonic development, developmental growth, and respiratory system development) are statistically enriched (p < 0.05) (Fig. 5d). These results suggest Prmt5 is implicated in FGSC identity, possibly through suppressing both terminal differentiation and the somatic program.
Germline stem cells are critical for passing genetic information from generation to generation. In our previous work we generated mouse FGSCs  and several studies have reported the generation of FGSCs in other mammalian species [10–12, 40]. Although the existence of FGSCs has been debated and such a cell population possibly represents remnants of early PGCs or the gonocyte stage , in our study several lines of evidence indicate there is a big difference between FGSCs and PGCs. Firstly, several PGC determinants (including Prdm1 and Prdm14) are not actively transcribed in FGSCs. Secondly, the transcriptome patterns of FGSCs and PGCs at various developmental stages differ to some extent (Additional file 1: Figure S8). Thirdly, although profound DNA demethylation occurs in late PGCs, we still observed obvious differences in DNA methylation patterns between E11.5 PGCs and FGSCs (Fig. 3a). These observations suggest it is less likely that FGSCs are remnants of PGCs.
Unipotency of FGSCs requires repression of the somatic program. Therefore, it is critical to understand how the somatic program is suppressed. Our previous work reported that the bivalent domain plays an important role in maintenance of ESC pluripotency, partially through transcriptional repression of the embryonic development program . In this study, we found that such a bivalent domain chromatin signature is less prevalent throughout the FGSC genome. Instead, we observed that DNA methylation is actively involved in repression of the somatic program (Fig. 3d). Moreover, we found that DNA methylation of developmental genes is present only in FGSCs and not in MGSCs (Fig. 4d). Similar observations were reported in zebrafish germ cells , suggesting that such gender-specific DNA methylation patterns are probably conserved between teleost vertebrates and mammals. Among genes with a methylated promoter, more than 90 % are also methylated at the gene body region and most of these genes are involved in development (Additional file 4: Table S4). Given the lower presence of bivalent genes in FGSCs, we speculate that FGSCs utilize alternative epigenetic mechanisms, such as methylation at both promoters (the repressive transcription mark) and gene bodies (the active transcription mark), to maintain developmental plasticity. In addition to DNA methylation, we found Prmt5 is also involved in such repression (Fig. 5d). These observations suggest that multiple layers of regulation restrict the somatic program in FGSCs.
The relatively simple characteristics of FGSC (self-renewal and unipotency) make it an ideal model for stem cell biology. Although the pluripotency genes Nanog, Sox2, Esrrb, and Tcl1  are repressed in both FGSCs and MGSCs , which suggests that GSCs may maintain unipotency by preventing the expression of the core pluripotency circuit, our recent work demonstrated that FGSCs could be converted into pluripotent stem cells . Here, we found several mechanisms are shared in both types of stem cells. A previous report demonstrated that Prmt5 associates with Mep50 and methylates cytosolic histone H2A (H2AR3me2s) to inhibit differentiation of ESCs . Similarly, our results indicate Prmt5 is involved in FGSC biology by repression of meiosis- and oogenesis-related genes (Fig. 5b, d). Although localized in cytoplasm, it remains to be elucidated whether Prmt5 forms a complex with Mep50 to exert the repression activity. In addition to Prmt5, Max was found to repress meiosis-related genes in ESCs and the expression of Stra8 and Sycp3 is significantly up-regulated in Max knockdown ESCs . Consistent with this observation, we found the expression of some meiosis-related genes (including Stra8, Sycp3, and Figla) is significantly up-regulated when Max was knocked down in FGSCs (Additional file 1: Figure S9). These observations suggest FGSCs and ESCs share some mechanisms to inhibit differentiation. Given the conserved mechanisms in various types of stem cells, studies on FGSCs, despite their relative simplicity, may provide insights into the mechanisms involved in other types of stem cells with complicated properties.
Our extensive epigenomic profiling analysis revealed that DNA methylation contributes to the unipotency of FGSCs primarily by suppressing somatic programs and is potentially involved in the maintenance of FGSC sexual identity. The genome-wide epigenetic signatures and the transcription regulators identified here provide an invaluable resource for understanding the fundamental features of mouse FGSCs.
FGSCs at passage number 32–35 were isolated as in our previous report  and cultured on gelatinized plates with minimum essential medium (MEM)-α containing 10 % fetal bovine serum, 1 mM non-essential amino acids, 2 mM L-glutamine, 1 mM sodium pyruvate, 0.1 mM β-mercaptoethanol (Sigma-Aldrich), 10 ng/ml mouse epidermal growth factor, 10 ng/ml mouse leukemia inhibitory factor, 40 ng/ml human glial cell line-derived neurotrophic factor, 1 ng/ml human basic fibroblast growth factor, and 15 μg/ml penicillin at 37 °C in a 5 % CO2 atmosphere as previously described .
Cells were fixed in 4 % paraformaldehyde for 15 min, washed twice in phosphate-buffered saline, and then incubated with 10 % goat serum at 37 °C for 15 min. Primary anti-Fragilis antibody (ab15592, Abcam, 1:200), anti-Mvh antibody (ab13840, Abcam, 1:100) or anti-Prmt5 (ab109451, Abcam, 1:50), and secondary antibody IgG (SA00003-2, Proteintech, 1:200) were used for immunostaining. Images were acquired using a Nikon A1Si confocal microscope or Leika DMI 3000 B inverted microscope.
Total RNA was extracted using Trizol reagent (Ambion) according to the manufacturer’s instructions. First-strand cDNA was transcribed using 4 μg total RNA and SuperScript™ III reverse transcriptase (Invitrogen) following the manufacturer’s protocol. We used a 20th of the RT reaction product for PCR amplification as described previously . Primers for each gene are provided in Additional file 1: Table S7.
The preparation of ChIP and input DNA libraries were performed as previously described . Briefly, cells were crosslinked with 1 % formaldehyde for 10 min at room temperature and quenched with 125 mM glycine. The fragmented chromatin fragments were pre-cleared and then immunoprecipitated with Protein A + G Magnetic beads coupled with anti-H3K4me1 (ab8895, Abcam), anti-H3K4me3 (ab8580, Abcam), anti-H3K27ac (ab4729, Abcam), anti-H3K27me3 (07–449, Millipore), and anti-RNA Pol II (ab5131, Abcam) antibodies. After reverse crosslinking, ChIP and input DNA fragments were end-repaired and A-tailed using the NEBNext End Repair/dA-Tailing Module (E7442, NEB) followed by adaptor ligation with the NEBNext Ultra Ligation Module (E7445, NEB). The DNA libraries were amplified for 15 cycles and subjected to deep sequencing with an Illumina HiSeq 2000.
Methylated DNA was prepared with a MethylCap Kit (AF-100-0048, Diagenode) according to the manufacturer’s instructions. MethylCap and input DNA libraries were sequenced with an Illumina HiSeq 2000.
Total RNA was extracted using Trizol (Ambion Life Technologies) according to the Ambion standard RNA isolation procedure and treated with TURBO DNase (AM2238, Ambion). Poly A+ RNA was purified using the Oligotex mRNA Mini Kit (70022, QIAGEN). Then the mRNA library was constructed with a NEBNext Ultra Directional RNA Library Prep Kit for Illumina (E7420S/L, NEB) and sequenced on an Illumina HiSeq 2000.
RNA-mediated interference and quantitative RT-PCR analysis
Small interfering RNAs (siRNAs) targeting Dnmt1, Prmt5, and Max and control siRNA (Additional file 1: Table S8) were transfected using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions. Briefly, FGSCs were plated in growth medium without antibiotics one day before transfection. Transfection was performed at day 1 and day 3. For gene knockdown analysis, the cells were harvested 48 h after the second transfection and RNA was extracted using Trizol reagent according to the protocol provided by the manufacturer (Ambion Life Technologies). For reverse transcription, 5 μg of total RNA, 250 ng of random hexamer primers, and 200 U of SuperScript III (Invitrogen) were used in a 20-μl reaction volume. Quantitative PCR (qPCR) analysis was carried out with Maxima SYBR Green/ROX qPCR Master Mix (K0222, Thermo Scientific) on a StepOnePlus qPCR machine (Applied Biosystems).
All histone modification ChIP-seq, DNA methylation, and control raw data reads were mapped using Bowtie (version 1.0.1) to the UCSC mm10 genome reference . The normalized coverage was calculated by binning the unique tags in 50-bp bins and the number of reads in each bin was normalized using reads per kilobase per million reads (RPKM). We identified the enriched ChIP-Seq regions over background with the MACS version 1.4.2 (model-based analysis of ChIP-Seq) peak finding algorithm . The parameters of MACS were set as default except --nomodel = T, --shiftsize = 75. In addition, the p value cutoff was set as 1.0E-3 for H3K27me3 calling. The DNA methylation data were processed with the MEDIPS method  with default parameters. To obtain the K-means results, we blurred the ChIP-Seq data with a rectangular mean kernel and applied a square root transformation to reduce the effect of noise and outliers. GO analysis was performed with GREAT  or DAVID .
For the purpose of comparison and clustering, other data were downloaded from the Gene Expression Omnibus (GEO) database. Mouse ESC ChIP-Seq data for H3K4me1, H3K4me3, and H3K27ac were obtained from Shen et al.  (GEO accession number GSE29218) and for H3K27me3 from Ng et al.  (accession number: GSE38164). The MeDIP-Seq and RNA-Seq data of mouse ESCs were obtained from Yu et al.  (GEO accession number GSE38596) and Surface et al.  (GEO accession number GSE53208), respectively. The E11.5 PGC MeDIP-Seq data were taken from Hackett et al.  (Sequence Read Archive accession number SRA060914). The adult male germline stem cell BiSeq data were obtained from Hammoud et al.  (GEO accession number GSE49624). The E9.5, E11.5, and E13.5 PGC RNA-Seq data were taken from Yamaguchi et al. [55, 56] (GEO accession number GSE41908).
For comparison of FGSC and MGSC DNA methylation datasets, we used the strategy in a previous report which allows comparison of methylation datasets generated by different approaches . Briefly, we first divided the UCSC known gene promoter regions into 500-bp windows and calculated the methylation level in each window and then calculated the Pearson correlation coefficient between them. To examine the methylation levels in FGSCs, we evaluated them with the relative methylation score (rms), calculated with the R package MEDIPS; for the MGSC whole-genome bisulfate sequencing dataset, the absolute methylation signal (ams), calculated with the R package MethlKit, was used. To further analyze the difference, we divided the genome into 1-kb tiling windows and defined the highly methylated regions in each cell population. For FGSCs, the MEDIPS package was used and the regions that met the criteria (false discovery rate (FDR) adjusted p value <0.01 and fold change >2) were defined to be highly methylated. For MGSCs, the exact binomial test was used to test the significance of the ratio of C/(C + T); regions with a ratio significantly larger than 0.25 (FDR-adjusted p value <0.01) were regarded as methylated and those with a ratio significantly larger than 0.75 (FDR-adjusted p value <0.01) were regarded as highly methylated.
ChIP-Seq, chromatin immunoprecipitation sequencing; DMR, differentially methylated region; ESC, embryonic stem cell; FDR, false discovery rate; FGSC, female germline stem cell; GEO, Gene Expression Omnibus; GO, gene ontology; GREAT, Genomic Regions Enrichment of Annotations Tool; GSC, germline stem cell; MGSC, male germline stem cell; PGC, primordial germ cell; piRNA, piwi-interacting RNA; qPCR, quantitative polymerase chain reaction; RNA-Seq, RNA sequencing; RT-PCR, reverse transcription polymerase chain reaction; TSS, transcription start site
Lehmann R. Germline stem cells: origin and destiny. Cell Stem Cell. 2012;10:729–39.
Surani MA, Hayashi K, Hajkova P. Genetic and epigenetic regulators of pluripotency. Cell. 2007;128:747–62.
Morgan HD, Santos F, Green K, Dean W, Reik W. Epigenetic reprogramming in mammals. Hum Mol Genet. 2005;14:R47–58.
Peters H. Migration of gonocytes into the mammalian gonad and their differentiation. Philos Trans R Soc Lond B Biol Sci. 1970;259:91–101.
Borum K. Oogenesis in the mouse: a study of the meiotic prophase. Exp Cell Res. 1961;24:495–507.
Anderson L, Hirshfield A. An overview of follicular development in the ovary: from embryo to the fertilized ovum in vitro. Md Med J. 1992;41:614–20.
McLaren A. Meiosis and differentiation of mouse germ cells. Symp Soc Exp Biol. 1984;38:7–23.
Zuckerman S. The number of oocytes in the mature ovary. Recent Prog Horm Res. 1951;6:63–108.
Zou K, Yuan Z, Yang Z, Luo H, Sun K, Zhou L, et al. Production of offspring from a germline stem cell line derived from neonatal ovaries. Nat Cell Biol. 2009;11:631–6.
Zhou L, Wang L, Kang JX, Xie W, Li X, Wu C, et al. Production of fat-1 transgenic rats using a post-natal female germline stem cell line. Mol Hum Reprod. 2014;20:271–81.
Johnson J, Canning J, Kaneko T, Pru JK, Tilly JL. Germline stem cells and follicular renewal in the postnatal mammalian ovary. Nature. 2004;428:145–50.
White YA, Woods DC, Takai Y, Ishihara O, Seki H, Tilly JL. Oocyte formation by mitotically active germ cells purified from ovaries of reproductive-age women. Nat Med. 2012;18:413–21.
Zou K, Hou L, Sun KJ, Xie WH, Wu J. Improved efficiency of female germline stem cell purification using Fragilis-based magnetic bead sorting. Stem Cells Dev. 2011;20:2197–204.
Rivera CM, Ren B. Mapping human epigenomes. Cell. 2013;155:39–55.
Bernstein BE, Mikkelsen TS, Xie X, Kamal M, Huebert DJ, Cuff J, et al. A bivalent chromatin structure marks key developmental genes in embryonic stem cells. Cell. 2006;125:315–26.
Zhao XD, Han X, Chew JL, Liu J, Chiu KP, Choo A, et al. Whole-genome mapping of histone H3 Lys4 and 27 trimethylations reveals distinct genomic compartments in human embryonic stem cells. Cell Stem Cell. 2007;1:286–98.
Rada-Iglesias A, Bajpai R, Swigut T, Brugmann SA, Flynn RA, Wysocka J. A unique chromatin signature uncovers early developmental enhancers in humans. Nature. 2011;470:279–83.
Pan Y. A new tool to generate transgenic rats using female germline stem cells from post-natal ovaries. Mol Hum Reprod. 2014;20:283–5.
Surface LE, Fields PA, Subramanian V, Behmer R, Udeshi N, Peach SE, et al. H2AZ1 monoubiquitylation antagonizes BRD2 to maintain poised chromatin in ESCs. Cell Rep. 2016;14:1142–55.
Lee J, Shinohara T. Epigenetic modifications and self-renewal regulation of mouse germline stem cells. Cell Res. 2011;21:1164–71.
Smith ZD, Meissner A. DNA methylation: roles in mammalian development. Nat Rev Genet. 2013;14:204–20.
Thorvaldsdottir H, Robinson JT, Mesirov JP. Integrative Genomics Viewer (IGV): high-performance genomics data visualization and exploration. Brief Bioinform. 2013;14:178–92.
Guenther MG, Levine SS, Boyer LA, Jaenisch R, Young RA. A chromatin landmark and transcription initiation at most promoters in human cells. Cell. 2007;130:77–88.
Heintzman ND, Hon GC, Hawkins RD, Kheradpour P, Stark A, Harp LF, et al. Histone modifications at human enhancers reflect global cell-type-specific gene expression. Nature. 2009;459:108–12.
Creyghton MP, Cheng AW, Welstead GG, Kooistra T, Carey BW, Steine EJ, et al. Histone H3K27ac separates active from poised enhancers and predicts developmental state. Proc Natl Acad Sci U S A. 2010;107:21931–6.
McLean CY, Bristor D, Hiller M, Clarke SL, Schaar BT, Lowe CB, et al. GREAT improves functional interpretation of cis-regulatory regions. Nat Biotechnol. 2010;28:495–501.
Rada-Iglesias A, Bajpai R, Prescott S, Brugmann SA, Swigut T, Wysocka J. Epigenomic annotation of enhancers predicts transcriptional regulators of human neural crest. Cell Stem Cell. 2012;11:633–48.
Ancelin K, Lange UC, Hajkova P, Schneider R, Bannister AJ, Kouzarides T, et al. Blimp1 associates with Prmt5 and directs histone arginine methylation in mouse germ cells. Nat Cell Biol. 2006;8:623–30.
Jones PA. Functions of DNA methylation: islands, start sites, gene bodies and beyond. Nat Rev Genet. 2012;13:484–92.
Varley KE, Gertz J, Bowling KM, Parker SL, Reddy TE, Pauli-Behn F, et al. Dynamic DNA methylation across diverse human cell lines and tissues. Genome Res. 2013;23:555–67.
Kocer A, Reichmann J, Best D, Adams IR. Germ cell sex determination in mammals. Mol Hum Reprod. 2009;15:205–13.
Murray SM, Yang SY, Van Doren M. Germ cell sex determination: a collaboration between soma and germline. Curr Opin Cell Biol. 2010;22:722–9.
Hammoud SS, Low DH, Yi C, Carrell DT, Guccione E, Cairns BR. Chromatin and transcription transitions of mammalian adult germline stem cells and spermatogenesis. Cell Stem Cell. 2014;15:239–53.
Bock C, Tomazou EM, Brinkman AB, Muller F, Simmer F, Gu HC, et al. Quantitative comparison of genome-wide DNA methylation mapping technologies. Nat Biotechnol. 2010;28:1106–14.
Coultas L, Bouillet P, Loveland KL, Meachem S, Perlman H, Adams JM, et al. Concomitant loss of proapoptotic BH3-only bcl-2 antagonists Bik and Bim arrests spermatogenesis. EMBO J. 2005;24:3963–73.
Meeks JJ, Weiss J, Jameson JL. Dax1 is required for testis determination. Nat Genet. 2003;34:32–3.
Warr N, Siggers P, Bogani D, Brixey R, Pastorelli L, Yates L, et al. Sfrp1 and Sfrp2 are required for normal male sexual development in mice. Dev Biol. 2009;326:273–84.
Waterston RH, Lindblad-Toh K, Birney E, Rogers J, Abril JF, Agarwal P, et al. Initial sequencing and comparative analysis of the mouse genome. Nature. 2002;420:520–62.
da Huang W, Sherman BT, Lempicki RA. Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc. 2009;4:44–57.
Bui HT, Van Thuan N, Kwon DN, Choi YJ, Kang MH, Han JW, et al. Identification and characterization of putative stem cells in the adult pig ovary. Development. 2014;141:2235–44.
De Felici M. Germ stem cells in the mammalian adult ovary: considerations by a fan of the primordial germ cells. Mol Hum Reprod. 2010;16:632–6.
Potok ME, Nix DA, Parnell TJ, Cairns BR. Reprogramming the maternal zebrafish genome after fertilization to match the paternal methylation pattern. Cell. 2013;153:759–72.
Yeo JC, Ng HH. The transcriptional regulation of pluripotency. Cell Res. 2013;23:20–32.
Wang H, Jiang M, Bi H, Chen X, He L, Li X, et al. Conversion of female germline stem cells from neonatal and prepubertal mice into pluripotent stem cells. J Mol Cell Biol. 2014;6:164–71.
Tee WW, Pardo M, Theunissen TW, Yu L, Choudhary JS, Hajkova P, et al. Prmt5 is essential for early mouse development and acts in the cytoplasm to maintain ES cell pluripotency. Genes Dev. 2010;24:2772–7.
Maeda I, Okamura D, Tokitake Y, Ikeda M, Kawaguchi H, Mise N, et al. Max is a repressor of germ cell-related gene expression in mouse embryonic stem cells. Nat Commun. 2013;4:1754.
Zhang Y, Yang Z, Yang Y, Wang S, Shi L, Xie W, et al. Production of transgenic mice by random recombination of targeted genes in female germline stem cells. J Mol Cell Biol. 2011;3:132–41.
Langmead B, Trapnell C, Pop M, Salzberg SL. Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol. 2009;10:R25.
Zhang Y, Liu T, Meyer CA, Eeckhoute J, Johnson DS, Bernstein BE, et al. Model-based analysis of ChIP-Seq (MACS). Genome Biol. 2008;9:R137.
Lienhard M, Grimm C, Morkel M, Herwig R, Chavez L. MEDIPS: genome-wide differential coverage analysis of sequencing data derived from DNA enrichment experiments. Bioinformatics. 2014;30:284–6.
Shen Y, Yue F, McCleary DF, Ye Z, Edsall L, Kuan S, et al. A map of the cis-regulatory sequences in the mouse genome. Nature. 2012;488:116–20.
Ng JH, Kumar V, Muratani M, Kraus P, Yeo JC, Yaw LP, et al. In vivo epigenomic profiling of germ cells reveals germ cell molecular signatures. Dev Cell. 2013;24:324–33.
Yu P, Xiao S, Xin X, Song CX, Huang W, McDee D, et al. Spatiotemporal clustering of the epigenome reveals rules of dynamic gene regulation. Genome Res. 2013;23:352–64.
Hackett JA, Sengupta R, Zylicz JJ, Murakami K, Lee C, Down TA, et al. Germline DNA demethylation dynamics and imprint erasure through 5-hydroxymethylcytosine. Science. 2013;339:448–52.
Yamaguchi S, Hong K, Liu R, Shen L, Inoue A, Diep D, et al. Tet1 controls meiosis by regulating meiotic gene expression. Nature. 2012;492:443–7.
Yamaguchi S, Hong K, Liu R, Inoue A, Shen L, Zhang K, et al. Dynamics of 5-methylcytosine and 5-hydroxymethylcytosine during germ cell reprogramming. Cell Res. 2013;23:329–39.
Geister KA, Brinkmeier ML, Hsieh M, Faust SM, Karolyi IJ, Perosky JE, et al. A novel loss-of-function mutation in Npr2 clarifies primary role in female reproduction and reveals a potential therapy for acromesomelic dysplasia, Maroteaux type. Hum Mol Genet. 2013;22:345–57.
Lim H, Paria BC, Das SK, Dinchuk JE, Langenbach R, Trzaskos JM, et al. Multiple female reproductive failures in cyclooxygenase 2-deficient mice. Cell. 1997;91:197–208.
The authors would like to thank Dr. Hong Sain Ooi of the Department of Biomedicine of Aarhus University for the technical assistance with data analysis. The authors are also grateful to Prof. Daniel M Czajkowsky for helpful discussion and careful reading.
This work was supported by Development Program for Basic Research of China [2013CB967402], Longhua Medical Project of State Clinical Research Center of TCM in Longhua Hospital [LYTD-21 and JDZX2012123] and State Key and National Natural Science Foundation of China [91229123 and 91019004].
Availability of data and materials
The datasets supporting the results of this article are available in the National Center for Biotechnology Information (NCBI) Sequence Read Archive under accession number SRP066132.
XZ and JW conceived and designed the experiments. XLZ, TS, JL, YG, CYN, and YK performed the experiments. JW, HL, CHW, and ZS carried out the data analysis. JW contributed reagents and materials. XLZ, JW, and XZ wrote the manuscript. JW and XZ led the study. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Ethics approval and consent to participate
All mouse experiments were approved by the Institutional Animal Care and Use Committee (IACUC) at Shanghai Jiao Tong University, Shanghai, China (SYXK (Shanghai 2007–0025)) and all experiments were carried out in accordance with the approved protocols.
Figures S1 to S9 and Tables S1, S7, and S8. (PDF 1366 kb)
GO analysis of genes related to FGSC-specific active enhancers. (XLSX 10 kb)
List of genes with bivalent promoters and histone modification regions (H3K4me3 or H3K27me3) in FGSCs. (XLSX 1417 kb)
List of genes methylated at promoters or gene bodies. (XLSX 115 kb)
List of genes methylated in FGSCs and/or MGSCs. (XLSX 180 kb)
Genes up- or down-regulated in Prmt5 kock-down FGSCs. (XLSX 733 kb)
About this article
Cite this article
Zhang, X., Wu, J., Wang, J. et al. Integrative epigenomic analysis reveals unique epigenetic signatures involved in unipotency of mouse female germline stem cells. Genome Biol 17, 162 (2016). https://doi.org/10.1186/s13059-016-1023-z
- Female germline stem cell