- Open Access
Isoform prefiltering improves performance of count-based methods for analysis of differential transcript usage
- Charlotte Soneson†1, 2,
- Katarina L. Matthes†3, 4,
- Malgorzata Nowicka1, 2,
- Charity W. Law5 and
- Mark D. Robinson1, 2Email author
© Soneson et al. 2016
Received: 11 October 2015
Accepted: 29 December 2015
Published: 26 January 2016
RNA-seq has been a boon to the quantitative analysis of transcriptomes. A notable application is the detection of changes in transcript usage between experimental conditions. For example, discovery of pathological alternative splicing may allow the development of new treatments or better management of patients. From an analysis perspective, there are several ways to approach RNA-seq data to unravel differential transcript usage, such as annotation-based exon-level counting, differential analysis of the percentage spliced in, or quantitative analysis of assembled transcripts. The goal of this research is to compare and contrast current state-of-the-art methods, and to suggest improvements to commonly used work flows.
We assess the performance of representative work flows using synthetic data and explore the effect of using non-standard counting bin definitions as input to DEXSeq, a state-of-the-art inference engine. Although the canonical counting provided the best results overall, several non-canonical approaches were as good or better in specific aspects and most counting approaches outperformed the evaluated event- and assembly-based methods. We show that an incomplete annotation catalog can have a detrimental effect on the ability to detect differential transcript usage in transcriptomes with few isoforms per gene and that isoform-level prefiltering can considerably improve false discovery rate control.
Count-based methods generally perform well in the detection of differential transcript usage. Controlling the false discovery rate at the imposed threshold is difficult, particularly in complex organisms, but can be improved by prefiltering the annotation catalog.
High-throughput sequencing of cDNA fragment populations, commonly known as RNA-seq, has rapidly become a default standard for profiling the composition of a transcriptome and quantifying the expression of individual transcriptional units (such as genes, transcripts, or exons). One of the key analysis challenges with RNA-seq data is to infer a set of such units that change their expression level or expression pattern between conditions. For example, identifying transcriptional changes that occur between normal and disease states may lead to markers of progression or prognosis, knowledge of molecules that can be pharmaceutically corrected, or to an understanding of the cascade of molecular events that have occurred. Typically, in unraveling interesting biological phenomena, inferring changes in expression is a critical, yet initial step of the discovery pipeline, and the results often feed into downstream interpretive analyses.
Several reports have recently provided snapshots of the performance of gene-level differential expression analysis methods, using both synthetic and experimental data [1–5]. As an example of the latter, Rapaport et al.  used large-scale experimental data from the SEQC (Sequencing Quality Control) consortium, consisting of replicates of well-known cell lines, to evaluate the performance of differential gene expression methods and the effects of modifying sequencing depths and the number of biological replicates. As an extension to this, the SEQC Consortium and Association of Biomolecular Resource Facilities recently finished comprehensive comparison studies with respect to accuracy, sensitivity, and reproducibility of gene-level measurements across sites, platforms, and algorithms [6, 7].
A well-placed recent review lists close to 100 computational methods that have been developed in the space of RNA-seq data and splicing analyses  and a few comparisons of DTU detection methods have been conducted. For example, a recent software review gives an overview of the features and interfaces available from recent tools to detect DTU, but no critical assessment of empirical performance is given . An early simulation study across a handful of popular mapping-DTU pipelines highlighted that: (i) the presence of DTU can increase false positive rates for standard gene-level differential expression analyses, which is perhaps not surprising, and (ii) in some cases, DTU simply cannot be detected with current methods . In that study, receiver operating characteristic curves were presented, but they give little sense of the false discovery rate (FDR) control during typical usage. More recently, Liu et al.  conducted a wide-ranging comparison of eight DTU detection methods using both simulated and experimental data. They compared performance across different alternative splicing ratios, read depths, dispersion patterns, types of splice changes, and sample sizes, and they explored the influence of annotation, highlighting that no single method dominates in performance and that they often give conflicting results. In the current study, we augment earlier studies in several important ways: we explore a spectrum of counting approaches, highlight striking differences in FDR control between simple and complex organisms, improve existing work flows by applying carefully designed filtering criteria, and explore the effect of incomplete annotation catalogs. The simulated data are available from ArrayExpress with accession number E-MTAB-3766.
Methods for DTU
Broadly speaking, there are three major classes of methods designed to detect DTU. First, the assembly-based (or isoform deconvolution) methods (e.g., the cufflinks/cuffdiff pipeline [15–17]) reconstruct and quantify the expression of a set of transcripts that best explain the observed reads. The cuffdiff test for DTU within a gene is based on the Jensen–Shannon divergence, measuring the similarity between two probability distributions. The second class of methods focuses on specific types of alternative splicing (e.g., retained introns or alternative exons) and identifies the number of observed reads that unambiguously support the presence or absence of each splicing event (e.g., rMATS ). Comparing these read counts gives an estimate of the percentage spliced in (psi value), which can then be compared between conditions for each event.
The third type of DTU detection methods do not directly quantify the transcript expression, but rather use differential exon usage as a surrogate to infer DTU. The genome is divided into (typically disjoint) counting bins and the number of observed reads overlapping each bin is counted. To infer differential exon (bin) usage between conditions, these methods often make use of (general or generalized) linear models containing an interaction term between the bin identifier and the condition of interest to search for non-proportionality of the bin counts within a gene between the conditions. Arguably, the most widely used differential exon usage detection method is implemented in the DEXSeq R package  but alternatives, such as the diffSplice function from the limma R package, are available . Since DEXSeq infers differential exon usage, it is left to the user to interpret which transcripts are differentially used, given the evidence for a particular exon bin–condition interaction. However, already knowing which exons are affected can lead to biologically meaningful interpretation of the functional impact of their differential usage (e.g., ).
Bin read counting
The read-counting function distributed with the DEXSeq package splits the coding parts of the genes into non-overlapping exon bins and counts the number of reads overlapping each of these bins. Reads that overlap multiple bins are assigned to all of them, which increases the correlation between the bin counts and implies that the sum of the bin counts can significantly exceed the number of sequencing reads in the experiment. With default settings, genes that overlap each other are aggregated into a composite gene complex containing all exon bins of the original genes. The identifier of this complex is obtained by combining the identifiers of the aggregated genes. In practice, this could lead to difficulties in result interpretation, and the differential splicing detection could potentially be affected by overall differential gene expression of a subset of the genes involved in a complex. In this study, we examine whether using alternative definitions of the counting bins, while keeping the interaction inference engine fixed, could provide benefits to the overall performance of DEXSeq. In total, we compare nine variants of counting bins. In this section, we give a brief description of the methods used and define the name that will be used to identify each method in the evaluations (given in parentheses). More details regarding the application of these methods can be found in Additional file 1, as well as in the project GitHub repository (https://github.com/markrobinsonuzh/diff_splice_paper). For more details regarding their theoretical underpinnings and implementation, refer to the original publications.
In addition to the default DEXSeq exon bin counting (DEXSeq-default) (DEXSeq R package v1.14.0) , we explore the effect of circumventing the merging of overlapping genes in two ways (see Additional file 1: Figs. S1 and S2 for illustrations). First, we change the arguments to the DEXSeq annotation preparation function to exclude overlapping exon parts on the same strand rather than aggregate genes (DEXSeq-noaggreg). This eliminates the composite feature identifiers, but may leave out important genomic regions that cannot be unambiguously assigned to one gene. This is particularly problematic if the annotation catalog is overpopulated with irrelevant transcripts. Second, we use functions from Bioconductor  to split the genes manually into disjoint counting bins, excluding overlapping parts even if they are on different strands and count the reads using the featureCounts function from the Rsubread R package (v1.18.0) (featureCounts-flat) [22, 23], allowing reads to be assigned to multiple features. We also explore the effect of completely circumventing the division of exons into disjoint bins and assign the reads to the original exons, again allowing a read to be assigned to multiple exons (featureCounts-exon). The SplicingGraphs R package (v1.8.1) provides a counting method where splicing graphs are constructed from the gene models provided and the reads are assigned to the edges of the graph (representing exons or introns) (SplicingGraph). Since the reads spanning exon junctions are expected to be the most informative for identifying DTU, we also explicitly evaluate the use of the junction counts generated by the TopHat aligner (v2.0.14), ignoring all reads that fall completely within exons (TopHat-junctions). Using MISO (v0.5.3) , we define the counting bins as combinations of isoforms and count the number of reads that align in positions compatible with each given combination (MISO). Yet another way of defining counting bins is provided by an intermediate representation from the casper R package (v2.2.0) , which defines the bins as the exon paths traced out by the two reads in a pair (casper). Finally, we define the counting bins as the isoforms themselves and use kallisto (v0.42.1)  to estimate the number of reads assigned to each isoform (kallisto).
Each of the counting methods generates a count matrix, where the rows correspond to counting bins and the columns to samples. These count matrices are used as the input to DEXSeq in searching for bins showing evidence of differential usage between conditions. Since each approach defines bins in a different way and not all bins are possible to unambiguously associate with a given isoform, we evaluate them in terms of their ability to detect differential isoform usage at the gene level.
Conceptually, each of the different counting bin definitions has advantages and disadvantages when used in conjunction with an inference engine such as DEXSeq, and ultimately the optimal choice likely depends on aspects such as the level at which interpretation can most easily be made, and the confidence in the annotation catalog. DEXSeq allows the user to determine which bin(s) are preferentially included or excluded in a given condition. In some situations, where the differential regulation of specific isoforms is expected to be involved in the phenotype determination, it can be advantageous to define the bins as the isoforms themselves. In other situations, however, the key phenotypic determinant may be the inclusion of a specific exon containing important genetic material, and the precise isoform contributing this exon is less important. In such cases, (sub)exon-specific bins may be preferable. In addition, using exon-level bins allows the detection of events (such as exon skipping) that are not annotated as part of an existing isoform, and isoform-level bins will not allow the detection of splicing aberrations in genes with a single isoform. On the other hand, exon-level bins could lead to spurious findings in terms of significantly differential bins that could not be the result of a true change in isoform usage.
Also other aspects, such as the degree of multi-counting, could lead to performance differences between the bin definition approaches. Typically, transcript abundance estimation methods, such as kallisto, attempt to distribute reads overlapping multiple transcripts to optimize a likelihood function. In contrast, the DEXSeq counting script assigns reads overlapping multiple bins to each of them, potentially increasing the correlation between bin counts within a gene. This multi-counting increases the count for the individual bins, particularly in situations where the bins are much shorter than the reads, and thus potentially leads to higher statistical power. On the opposite side of the spectrum, methods that consider only junction-spanning reads (such as TopHat-junctions) exclude a potentially large fraction of the reads and can, thus, be expected to lose power, especially when the exons are relatively long compared to the reads.
For methods based on the original (non-disjoint) exons (such as featureCounts-exon), we expect a lower power to detect switches between isoforms where the critical region (the genomic region that is unique to one or the other isoform) is small. This is because the reads from the critical region will contribute relatively little to the counts of the exons (bins). Thus, even dramatic relative changes in this small contribution may pass unnoticed, whereas they would be apparent if the critical region formed a bin itself (such as, for example, with DEXSeq-default, DEXSeq-noaggreg, and featureCounts-flat).
Results and discussion
We evaluated the counting methods outlined in the previous section (using DEXSeq as the inference engine), as well as cuffdiff (v2.2.1) and rMATS (v3.0.9), using simulated data based on the fruit fly and human as described in “Methods”. The characteristics of the two organisms are outlined in Additional file 1: Fig. S3 and Table S1. For each organism, we simulated reads for three replicates in each of two conditions. DTU was introduced for 1000 genes by reversing the relative abundances of the two most abundant isoforms in one of the conditions, while keeping the total number of transcripts generated from the gene constant (that is, no gene-level differential expression). This generated truly differential genes with a range of effect sizes (the difference in relative abundance between the two differentially used isoforms). The simulation framework is outlined in Additional file 1: Fig. S4, and comparisons of the average expression levels between the two conditions are shown in Additional file 1: Figs. S5 and S6. In Additional file 1: Figs. S7 and S8, we also provide a comparison of the overall count patterns for the different methods.
The performance evaluation is based on gene-level q values (adjusted p values) calculated as described in “Methods”. After thresholding the gene-wise q values at three common values (0.01, 0.05, and 0.1), we evaluated the true positive rate (TPR, the fraction of genes with true differential isoform usage that show q values below the threshold) and the observed FDR (the fraction of all genes with q value below the threshold where there is no true differential isoform usage). Any gene identifiers that were not present in the list of simulated genes were excluded from the evaluation, since these genes could not be classified as true positive (TP), true negative (TN), false positive (FP), or false negative (FN). This affected the performance estimates for casper and DEXSeq-default counts, as well as cuffdiff, since these methods sometimes form gene complexes, with identifiers that are combinations of the identifiers of the merged genes. The number of features (genes plus complexes) and the number of complexes for these methods are given in Additional file 1: Table S1.
Overall performance and effect of transcriptome complexity
The second lowest power was obtained by rMATS, mostly since it is designed to detect only simple splicing events (for these, however, the power was comparable to the other methods, see Additional file 1: Fig. S11). Among the counting methods used with DEXSeq, the exon path counts from casper showed the lowest power for both organisms. This could be attributed to a combination of the relatively low read count for the individual bins and the merging of features into complexes, which could not be directly matched with the list of truly differential or non-differential genes. The same merging effect was seen for cuffdiff and for DEXSeq-default (which therefore showed a lower TPR than DEXSeq-noaggreg), albeit not as strong since DEXSeq and cuffdiff only merge genes if they are on the same strand, while casper merges also overlapping genes on different strands. The merging effect was particularly pronounced in the human data, where a larger fraction of the genes are overlapping to some extent and thereby were aggregated into complexes (Additional file 1: Table S1). An alternative could be to evaluate the methods for only the genes for which a true status indicator and a q value were available (that is, to subset the truth and result tables to only these genes before the performance evaluation). This approach is explored in Additional file 1: Fig. S12. As expected, it improved the power of casper and DEXSeq-default substantially.
In the fruit fly simulation, the count matrices generated by featureCounts-exon and TopHat-junctions performed relatively poorly, while their results in the human simulation were more on a par with the other methods. The performance difference between the organisms may be associated with the relatively low junction counts in the fruit fly and with the observed inability of featureCounts-exon to detect switches between genetically similar isoforms, where the critical regions (the regions that are unique to one of the isoforms) are very small (see Additional file 1: Fig. S13 for an illustration). In both organisms, the SplicingGraph, DEXSeq-noaggreg, and featureCounts-flat counts performed similarly. This was not surprising since the counting bins were similar between these methods (exons and introns). The transcript-level counts obtained by kallisto also appeared to give good results when combined with the DEXSeq inference engine, especially for relatively simple genomes such as the fruit fly. Similarly, MISO counts provided good results in the fruit fly simulation, but showed a relatively high FDR for the human data. In addition to its low power due to the inability to detect complex splicing events, rMATS showed a higher FDR than the other methods. A closer examination of the FP events revealed that these are enriched with rare events (inclusion levels close to 0) and relatively depleted of events with inclusion levels in the middle range between 0 and 1 (see Additional file 1: Fig. S14).
Performance stratified by gene characteristics
Number of features (genes plus gene complexes), the number of genes covered by any of the features (# incl. genes), the number of counting bins, and the sum of all bin counts for various counting methods and for the complete and reduced annotation catalog. Randomly excluding 20 % of the transcripts from the annotation catalog generally led to a slight increase in the average bin count, with the most pronounced effects seen for the methods defining the counting bins as (combinations of) transcripts (kallisto and MISO). MISO and SplicingGraph consider only genes with at least two isoforms, which explains the lower number of features for these methods
# incl. genes
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Effect of annotation catalog incompleteness on counting methods
All methods evaluated in this study rely on known annotations. While both organisms chosen here have well-characterized transcriptomes, this is far from true for many non-model species. Here, we studied the effect of an incomplete annotation catalog on the power of detecting differential isoform usage, by excluding 20 % of the known transcripts from the reference gtf file (randomly chosen, but proportionally split between differentially used and non-differentially used). We then reran the analysis of the simulated data, starting from the TopHat read alignment step. We restricted this analysis to the counting methods combined with DEXSeq, since they provided the best performance above.
Number of features (genes plus gene complexes), the number of genes covered by any of the features (# incl. genes), the number of counting bins, and the sum of all bin counts for various counting methods and for the complete and reduced annotation catalog. Randomly excluding 20 % of the transcripts from the annotation catalog generally led to a slight increase in the average bin count, with the most pronounced effects seen for the methods defining the counting bins as (combinations of) transcripts (kallisto and MISO). MISO and SplicingGraph consider only genes with at least two isoforms, which explains the lower number of features for these methods (Continued)
# incl. genes
Tot count/# bins
Isoform prefiltering improves the observed FDR
We noted above that the empirical FDR was often much higher than the imposed FDR threshold, especially for genes with one largely dominating isoform and that this effect was consistent across most counting methods. In an attempt to understand better the reasons behind this high FDR, we compared the characteristics of the genes falsely called differentially spliced (FPs) to those of the TPs (correctly called genes with true DTU), the FNs (non-significant genes with true underlying DTU), and TNs (genes correctly called non-differential). We focused on the counts obtained by DEXSeq-noaggreg, due to their intrinsic link to the DEXSeq framework and their overall good performance in the previous evaluations. The most notable observation was that the FP genes showed a larger variance among the dispersion estimates for their respective exon bins than the other gene categories (Additional file 1: Figs. S20 and S21). The effect was more pronounced for genes with many counting bins and genes with one highly dominant isoform. We hypothesized that reducing the number of bins by an initial filtering step, eliminating the non-expressed isoforms from the annotation catalog used to generate the counting bins, could improve the FDR control. Based on the RSEM-estimated isoform percentages from which the individual sample isoform percentages were derived, we thus generated four new annotation files for the DEXSeq counting by excluding all isoforms with relative abundance below (in turn) 5 %, 10 %, 15 %, and 25 % in both conditions. Then, we performed the counting and differential exon usage testing as before. Other filtering paradigms could be imagined, such as successively excluding the lowest expressed isoforms as long as their total relative abundance does not exceed a fixed threshold. For all the thresholds we evaluated, the reduction in the number of bins was substantially larger than the relative reduction in total bin counts (Additional file 1: Tables S2 and S3), which suggests a high degree of redundancy and/or that few reads were assigned to the excluded bins. The simulated human data contained a larger fraction of non-expressed isoforms (Additional file 1: Table S1) and thus, the reduction in the number of retained isoforms was larger than for the fruit fly simulation. It is worth highlighting that isoform-level filtering is not equivalent to filtering bins out of the count table (see Additional file 1: Figs. S22–S26 for a comparison to bin filtering approaches).
In our simulation study, we were able to use the underlying isoform abundances in the filtering. Of course, in experimental data sets, the true relative isoform abundances are not known and they may vary between samples and conditions. A possible approach with real data could be to estimate first the relative isoform abundance using, e.g., kallisto  or RSEM , and exclude isoforms where the relative abundance estimate does not exceed a certain fraction in any of the samples studied. This approach is evaluated in Additional file 1, along with several other filtering paradigms based on the observed exon bin counts (Additional file 1: Figs. S22–S26). There, we show that the isoform-guided filtering, based on estimated abundances, performs equally well. Another potential complication in experimental data is that lowly abundant isoforms can also be subject to differential usage and so a low filtering threshold may exclude differentially used isoforms. However, depending on the experiment, the reduction in FDR may compensate for this loss.
In this study, we used simulated data to evaluate nine counting methods in terms of their compatibility with the DEXSeq inference engine in the detection of DTU. The main motivation was to evaluate whether a non-standard counting approach could improve the performance of a well-established statistical inference method and to what extent the counting protocol influences the performance. Importantly, we did not attempt to quantify or compare the correctness of the counts obtained by the different methods, or their potential compatibility with other inference frameworks. We also compared the counting methods to one assembly-based method (cuffdiff) and one event-based method (rMATS). Our results show that the inference framework provided by DEXSeq, testing for the differential inclusion of particular counting bins, works well in conjunction with many different bin definitions, from sub-exonic bins quantified using data aligned to the genome (e.g., DEXSeq-noaggreg) to full-length transcript bins quantified using alignment-free approaches (e.g., kallisto). DEXSeq-noaggreg has the distinct advantage that some types of unannotated events (e.g., exon skipping) can still be detected with default settings, whereas kallisto has a definite speed advantage. We expect that, despite the recent excitement around ultrafast alignment-free quantification methods, many researchers will still elect to place reads in genomic context using alignment-based methods for visualization and interpretation. Ultimately, the choice of bin type should be guided by the level (exon or transcript) at which relevant inferences about the underlying biology can be made.
The characteristic that most strongly influenced the power to detect differential isoform usage was, unsurprisingly, the magnitude of the change in proportions between the differentially used isoforms. Also the complexity of the gene, that is, the number of isoforms, had a noticeable impact on the specificity and many complex but truly non-differentially spliced genes were falsely considered differential. Taken together, the count-based methods showed strong performance compared to the other methods. The best performance was obtained with the exon bin counts from DEXSeq-noaggreg (that, is the standard DEXSeq counting pipeline but without the default aggregation of overlapping genes) and featureCounts-flat. These methods provided a solid performance independently of the alternative splicing event type, the degree of similarity between the differentially used isoforms, the overall expression level and the number of isoforms for the genes, and the distribution of relative isoform abundances. The default DEXSeq counting, which aggregates overlapping genes into complexes, showed worse performance than counting without the aggregation step. This was mostly due to problems matching the identifiers of the differentially spliced genes detected (complexes) with the list of truly differentially spliced ones, which could also be a problem when interpreting results from an experimental data set.
SplicingGraph, MISO, and kallisto, which explicitly make use of the transcript structure to generate the counting bins, worked as well as the exon bin counts as long as the annotation catalog was complete, but the default exclusion of genes with a single annotated isoform (SplicingGraph and MISO), or the generation of only a single counting bin for those genes (kallisto) was detrimental with a relatively simple transcriptome (fruit fly) and an incomplete transcript catalog. The exon path counts from casper showed low TPRs, due to a combination of the low counts for each path and an aggregation of identifiers for overlapping genes, similar to that of DEXSeq-default. TopHat-junctions counts showed poor performance in the fruit fly simulation, especially for genes with few isoforms, which may be due to the relatively long fruit fly exons, implying a low number of reads spanning exon junctions. Using featureCounts on the original exon level (that is, without flattening the reference annotation) gave poor results when the counts were analyzed with DEXSeq, especially for the fruit fly simulation. These counts were not able to capture differential isoform usage when the differentially used isoforms were similar and were generally unable to detect most common simple alternative splicing event types.
By comparing the results from the human and fruit fly simulations, we have shown that the performance of the counting methods as well as the inference framework in general is dependent on the characteristics and annotation completeness of the underlying transcriptome. The higher complexity of the human transcriptome led to a higher overall FDR than for the fruit fly simulation, especially for genes with one dominant transcript. In the fruit fly genome, with long exons and few transcripts per gene, incomplete annotation had a detrimental effect on the power to detect differentially used isoforms while in the human simulation, where the exons are shorter and the number of transcripts per gene is much larger, the redundancy dampened the effect of missing annotations considerably. The negative impact of missing annotation entries could potentially be remedied by extending the annotation catalog using tools such as cufflinks , although it may not always be unambiguously clear if a newly assembled isoform represents a variant of a gene already existing in the annotation (and if so, which one) or if it should be considered separately.
Finally, we showed that the high FDR obtained especially for genes with one largely dominant isoform could be substantially improved by a simple prefiltering of the isoform catalog before the construction of the counting bins, with negligible loss of power. Excluding lowly abundant isoforms in this way led to a tangible reduction in the number of counting bins, with only a small reduction in the number of reads assigned to the genes. This type of filtering can also be biologically justified, given previous studies that have suggested that noisy or erroneous splicing is responsible for the majority of very low-abundance isoforms [30, 31]. Our evaluations suggest that the proposed isoform-guided filtering leads to better results than filtering of counting bins after the counting, which is the current standard procedure.
The analyses in this study are based on simulated data, due to the lack of publicly available, extensively validated experimental data sets for splicing analyses. By using experimental data to estimate the transcript abundances underlying the simulation, we capture aspects such as the number and relative expression of isoforms within each gene, and the correlation structure of expression levels between genes. The choice of RSEM as the simulator of transcript expression was motivated by its speed, ease of use, and flexibility, as well as the ability to estimate and incorporate sequencing biases and error structure from experimental data. However, experiments with other simulation engines (not shown) gave very similar results and we do not expect the choice of simulator to have a major impact on the presented results. All comparisons between methods are performed on the gene level, since this is the least common denominator of the different bin definition approaches and since, as discussed in the introduction, the choice of bin definition in a real scenario depends largely on the level at which relevant interpretation can be made. Also note that DTU, which is the topic of this paper, could also affect transcripts that are not themselves differentially expressed, since it focuses on the relative abundance of a gene’s isoforms.
The simulated data we used are made available in standard formats with accompanying metadata (see “Availability of supporting data” below), and thus our performance benchmark can be readily extended as new innovations are made to methods. The supplementary website also gives a link to a web application that facilitates the recreation of the performance comparisons shown in our study.
This section describes in detail the simulation procedure employed to generate the fastq files that are the basis for the evaluation of the various methods (see Additional file 1: Fig. S4 for a schematic illustration). We performed one simulation for the human and one for the fruit fly, following the same principles. For each organism, we simulated data from two conditions, each with three biological replicates. These two organisms were chosen since their transcriptomes show different characteristics, which could potentially have a large impact on the performance of the evaluated methods (Additional file 1: Fig. S3 and Table S1). Most strikingly, the fruit fly has considerably longer exons and transcripts than the human, while human genes typically have a much larger number of isoforms per gene. Moreover, they are model organisms for which the transcriptome catalog can be expected to be at least reasonably well characterized and, as such, the results translate to many real studies. However, we emphasize that the results are not expected to be restricted to these organisms, and we make our code available to facilitate extensions to other organisms.
The simulation was performed using RSEM (v1.2.21) , which, given transcripts per million (TPM) expression values for each isoform in a given reference annotation catalog, simulates the sequencing process and generates fastq files. To get realistic values for the expression levels and relative isoform abundances, as well as for the sequencing parameters, we used RSEM to estimate these values from real RNA-seq data sets (by means of the rsem-calculate-expression module). For the human simulation, we used the fastq files from the sample SRR493366 (http://www.ebi.ac.uk/ena/data/view/SRR493366) and for the fruit fly simulation, we used those from the sample SRR1501444 (http://www.ebi.ac.uk/ena/data/view/SRR1501444). These samples both represent paired-end sequencing experiments with a read length of 101 bp. The transcriptome catalogs used as the basis for the expression estimation and simulation were Ensembl GRCh37.71 for the human simulation (using only the chromosomes in the primary genome assembly) and BDGP5.70 for the fruit fly simulation. For both organisms, we restricted the simulation to protein-coding genes. Applying the RSEM expression estimation to the real data files provided us with a model file (detailing the sequencing settings and error model) and an isoform summary file (containing, among other things, the estimated TPM, expected count, and relative abundance for each isoform). We modified the model file slightly to avoid simulating reads where most bases are of very low base quality.
We used the isoform-level count and TPM estimates provided by RSEM as the basis for the generation of sample-wise TPM values for the six samples. First, we summed the estimated counts for all isoforms of each gene, scaled to the desired library size (40 million for the human and 25 million for the fruit fly) and used a mean-dispersion relationship derived from real data (see ) to generate a matching negative binomial dispersion value for each gene. The gene count for each sample was sampled from a negative binomial distribution with the estimated mean and dispersion parameters. We also calculated the gene-level read count per kilobase (RPK) by dividing the simulated read count by the effective gene length estimated by RSEM. Next, we simulated relative isoform abundances for each sample using a Dirichlet distribution with parameters set to the isoform fractions estimated by RSEM multiplied by a scale factor of 100. We selected 1000 genes to be affected by differential isoform usage between the two conditions. These genes were selected randomly among the genes with at least two expressed isoforms (with relative isoform abundance above 10 %) and a high enough expression level (expected gene count above 500). For each of these 1000 genes, the relative isoform abundances of the two most abundant isoforms were reversed in the second condition (samples 4–6). This type of switch event was studied in detail in a previous publication  where it was shown that many genes underwent this type of modification between conditions. From the isoform percentages and the previously estimated gene RPKs, we estimated the isoform RPKs by multiplication. Finally, the isoform TPM was obtained by scaling the RPK value. These transcript TPM values were used as the input to RSEM for simulating fastq files.
Generation of fastq files and mapping
Given the TPM estimates for the individual samples and the modified RSEM model file, we used the rsem-simulate-reads module of RSEM to generate paired fastq files for the six samples. We simulated 40 million read pairs for each human sample and 25 million pairs for each of the fruit fly samples. Of these reads, 5 % were simulated to come from a non-specific background (and, thus, not stem from any transcript). The fastq files were aligned to the reference genome and transcriptome using TopHat (v2.0.14) , provided with the reference gtf file.
To mimic the situation where the full transcriptome catalog of a studied organism is not known, we generated incomplete reference gtf files by excluding 20 % of the transcripts (proportionally distributed between differentially used and non-differentially used). The entire data analysis pipeline, starting from the TopHat alignment, was rerun with these incomplete gtf files (using the same set of simulated data files).
Detection of differential counting bin usage
Each of the counting methods applied in this study (see Additional file 1 for a more detailed description) generates a count matrix, where each row corresponds to a counting bin and each column corresponds to one of the six samples. They also contain information for grouping the counting bins together based on their gene of origin. Each count matrix is submitted to the DEXSeq R package (v1.14.0) to test for changes in relative usage of each counting bin between the two simulated conditions. A difference in relative counting bin usage is interpreted as a preferential inclusion or exclusion of that bin in one condition compared to the other, which we interpret as evidence in favor of differential isoform usage between the conditions. More technically, for each counting bin, given the number of reads assigned to the bin and the sum of the reads assigned to all other bins of the gene in each of the samples, DEXSeq fits generalized linear models to test for the presence of an interaction between the condition and the counting bin factor (this bin vs all others). The bin counts are assumed to follow a negative binomial distribution and the dispersion parameters are estimated from the data and subjected to shrinkage using the method implemented in the DESeq2 R package . Finally, a p value for each bin summarizes the evidence in favor of differential usage of the bin between the conditions.
Given the statistical test results for each counting bin, we use the perGeneQValue function from the DEXSeq package to summarize the results on the gene level. This function associates a q value with each gene by examining the number of genes for which the null hypothesis is rejected for at least one bin. DEXSeq applies an independent filtering step (adapted from DESeq2) and excludes counting bins with expression values that are too low from the testing. Genes for which all bins are filtered out are not assigned a q value and are, therefore, not included in our evaluations. Depending on the number and structure of the counting bins, the collections of genes for which DEXSeq can assign a q value differ between the counting methods. However, this does not affect the calculation of the observed TPR and FDR, since the excluded genes are not called differential.
Like DEXSeq, rMATS provides one p value per evaluated event (there may be multiple events for the same gene). These sub-gene structure p values were summarized into gene-level q values, quantifying the statistical evidence in favor of any differential usage of the counting bins of the gene, using the perGeneQValue function from DEXSeq. For cuffdiff, we used the gene-wise FDR estimates from the cds.diff output file. Importantly, this file records only differences in coding output between conditions, and genes where the coding sequences of the differentially used isoforms are identical will, therefore, not be detected with cuffdiff.
No approvals were required for the study.
Availability of supporting data
The data sets supporting the results of this article are available in the ArrayExpress repository with accession number E-MTAB-3766 [http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-3766/]. All code needed to reproduce the results are available from the project GitHub repository: https://github.com/markrobinsonuzh/diff_splice_paper. The list of truly differential genes and the final results (gene q values) for each method are available from the accompanying website: http://imlspenticton.uzh.ch/robinson_lab/splicing_comparison/. The web page also holds a link to a Shiny app that can take these summary files and reproduce the main figures of the manuscript.
The authors wish to thank Alejandro Reyes and members of the Robinson lab for helpful discussions and careful reading of the manuscript. We wish to acknowledge funding from an Swiss National Science Foundation (SNSF) Project Grant (143883), the European Commission through the 7th Framework Collaborative Project RADIANT (grant agreement number 305626), and the SNSF-funded National Centre of Competence in Research (NCCR) in RNA and Disease.
Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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