- Open Access
Loss of LIN-35, the Caenorhabditis elegansortholog of the tumor suppressor p105Rb, results in enhanced RNA interference
© Lehner et al.; licensee BioMed Central Ltd. 2006
Received: 7 September 2005
Accepted: 16 December 2005
Published: 20 January 2006
Genome-wide RNA interference (RNAi) screening is a very powerful tool for analyzing gene function in vivo in Caenorhabditis elegans. The effectiveness of RNAi varies from gene to gene, however, and neuronally expressed genes are largely refractive to RNAi in wild-type worms.
We found that C. elegans strains carrying mutations in lin-35, the worm ortholog of the tumor suppressor gene p105Rb, or a subset of the genetically related synMuv B family of chromatin-modifying genes, show increased strength and penetrance for many germline, embryonic, and post-embryonic RNAi phenotypes, including neuronal RNAi phenotypes. Mutations in these same genes also enhance somatic transgene silencing via an RNAi-dependent mechanism. Two genes, mes-4 and zfp-1, are required both for the vulval lineage defects resulting from mutations in synMuv B genes and for RNAi, suggesting a common mechanism for the function of synMuv B genes in vulval development and in regulating RNAi. Enhanced RNAi in the germline of lin-35 worms suggests that misexpression of germline genes in somatic cells cannot alone account for the enhanced RNAi observed in this strain.
A worm strain with a null mutation in lin-35 is more sensitive to RNAi than any other previously described single mutant strain, and so will prove very useful for future genome-wide RNAi screens, particularly for identifying genes with neuronal functions. As lin-35 is the worm ortholog of the mammalian tumor suppressor gene p105Rb, misregulation of RNAi may be important during human oncogenesis.
Introduction of double-stranded RNA (dsRNA) into metazoan cells results in the sequence specific degradation of messenger RNA in a process known as RNA interference (RNAi) . Components of the RNAi machinery are also involved in the regulation of endogenous gene expression, for example, in the silencing of repetitive DNA sequences and in the processing of microRNAs . RNAi has proved a very powerful tool to examine gene function . In Caenorhabditis elegans, RNAi is now routinely used to systematically examine in vivo loss of function phenotypes on a genome-wide scale. The efficiency of RNAi in C. elegans varies from gene to gene, however, such that the observed RNAi phenotype often does not represent the true null phenotype of a gene. In particular, genes expressed in neurons appear largely refractory to RNAi, which has precluded the use of RNAi screens to identify genes with neuronal functions . For this reason there has been great interest in identifying worm strains that display an enhanced sensitivity to RNAi. Previously, mutations in two genes have been shown to enhance RNAi sensitivity in C. elegans. These genes are predicted to function in dsRNA synthesis or turnover, and encode a putative RNA-dependent RNA polymerase (rrf-3 ) and a ribonuclease (eri-1 ). A genome-wide RNAi screen in an rrf-3 mutant strain identified 393 additional genes with RNAi phenotypes because of its increased sensitivity to RNAi . Here we report that inactivation of a subset of genes that function in the LIN-35/p105Rb chromatin-remodelling pathway also result in RNAi hypersensitivity in C. elegans. Indeed, we found that a straincarrying a null allele in lin-35 is more sensitive to RNAi than either rrf-3 or eri-1 mutant animals, making this strain an invaluable resource for future genome-wide RNAi screens.
Results and discussion
Loss of LIN-35 results in enhanced RNAi
The loss of function phenotypes generated by RNAi, like those generated by classic genetics, are highly dependent on the genetic background - many genes have very different RNAi phenotypes in a wild-type worms from those seen in animals mutant for a specific gene. Such genetic interactions can provide insight into how genes are organised into pathways. To begin to map out genetic interactions in the signal transduction and transcriptional networks that underpin C. elegans development, we used RNAi to individually target approximately 1,700 genes and compare the phenotypes generated in wild-type animals with the phenotypes in each of about 40 mutant strains; each strain carries a mutation in a key signalling component or chromatin regulator (B.L, C.C, J.T, A.F and A.G.F, manuscript submitted). The approximately 1,700 genes targeted encode the great majority of genes involved in signal transduction and transcriptional regulation, as annotated in Kamath et al.  (Additional data file 1). During this screening, we noticed that the RNAi phenotypes of many genes that had weak phenotypes in wild-type animals were greatly enhanced in the strain lin-35(n745); this carries a putative null mutation in the p105Rb ortholog, lin-35 . In this strain, the sterility and/or embryonic lethality of approximately 30% of all genes that had a weak phenotype in wild-type worms were enhanced (78 genes; Additional data file 2). Furthermore, 35 genes that had no detectable phenotype in wild-type worms had strong phenotypes in lin-35 mutants (Additional data file 2). In particular, many RNAi clones that result in partial F1 embryonic lethality in wild-type worms have complete P0 sterility or growth arrest in lin-35(n745) worms, suggesting a more rapid and complete inhibition of gene expression in the absence of lin-35 function.
The difference in RNAi phenotype for any gene that we observe in lin-35(n745) compared with wild-type could formally result either from an increase in RNAi sensitivity in the mutant or through some more complex genetic interactions (for example, through genetic buffering between lin-35 and a target gene). We believe the principal effect is through an increase in RNAi sensitivity for four reasons.
First, for genes that have a nonviable RNAi phenotype in lin-35(n745), the genetic null allele is also always nonviable, when known (35 genes) (Additional data file 3), suggesting that the stronger phenotype represents a near-null state.
Enhanced post-embryonic RNAi phenotypes observed in lin-35(n745) worms
RNAi induced silencing of lin-35 or lin-15B enhances the dsRNA-induced silencing of a GFP transgene
GFP expression partial‡
% Complete expression
Identification of genes that genetically interact with the lin-35 pathway
Enhanced RNAi phenotype
lin-35animals are more sensitive to RNAi than previously described RNAi hypersensitive strains
We compared the RNAi sensitivity of animals carrying strong loss-of-function mutations in the two previously described genes that are known to negatively regulate RNAi in C. elegans, rrf-3 or eri-1, to that of lin-35(n745) animals. rrf-3(pk1426) and eri-1(mg366) enhanced the RNAi phenotypes of 70 and 69 of 1,749 genes tested, respectively, compared to 113 genes enhanced by lin-35(n745) (Figure 1a; Additional data file 2). Every gene displaying an increased phenotype with rrf-3(pk1426) or eri-1(mg366) also has an increased RNAi phenotype with lin-35(n745). In addition, many genes that have enhanced RNAi phenotypes in rrf-3(pk1426) or eri-1(mg366) have even stronger phenotypes in lin-35(n745).
Although the RNAi clones that we tested in each of the four strains represented a functionally biased set of genes, we also found very similar results when using random RNAi clones targeting genes with many diverse functions. In addition to the approximately 1,800 RNAi clones originally screened, we also screened the first 682 RNAi clones targeting genes on C. elegans chromosome III. These genes have very diverse molecular functions (Additional data file 4) and we found that 42 of these clones also had RNAi phenotypes that were stronger in lin-35(n745) than in rrf-3(pk1426) worms (Additional data file 5). In addition, it is not just the number of genes with enhanced RNAi phenotypes that is greater in lin-35 than in the other strains; the strengths of the RNAi phenotypes are also enhanced. For example, 11 of the genes we tested from chromosome III had an RNAi phenotype in rrf-3 worms that was further enhanced in lin-35 worms (Additional data file 5).
These results show that lin-35(n745) worms are more sensitive to RNAi than any previously described single mutant strain and are an ideal strain for new RNAi-based screens. This is a key finding - merely finding another hypersensitive strain is not a particularly useful research tool unless it is an improvement on the previously identified strains. Our ranking of the three strains is based on the use of a large set of test genes, and thus our conclusion is robust and not a curiosity of a few atypical RNAi phenotypes. We note, however, that Wang et al.  also provide evidence that a lin-35(n745); eri-1(mg366) double mutant strain may display a further enhancement in RNAi sensitivity to lin-35(n745), suggesting that these two genes may partially function in parallel.
lin-35(n745)animals display increased sensitivity to RNAi in the nervous system
For unknown reasons, many neuronally expressed genes appear largely refractory to RNAi in wild-type worms, precluding reverse genetic analyses . We generated strong phenotypes for several neuronally expressed genes in lin-35(n745) animals (Table 1), suggesting RNAi-based screens for neuronal functions might be feasible in this strain. To test further for enhanced RNAi sensitivity in the nervous system of lin-35(n745) animals, we focused on genes expressed in the six touch receptor neurons of C. elegans. These neurons sense gentle touch to the body, and several mechanosensory abnormal (mec) genes have been identified that are needed for their development or function [17, 18]. Although RNAi has been detected in these neurons when dsRNA is injected into animals , it is not seen when dsRNA is delivered by feeding in wild-type animals (AC, C Keller, and MC, unpublished data), rendering high-throughput RNAi screens impractical.
We tested the touch sensitivity of wild-type and lin-35(n745) animals fed on bacteria targeting eight mec genes (mec-2, mec-3, mec-4, mec-8, mec-9, mec-10, mec-12 and mec-18) and two unrelated genes (gfp and sym-1). In wild-type worms, none of the bacterial strains caused touch insensitivity - that is, the Mec phenotype - either in adults that had fed on the bacteria throughout their entire larval development or in their progeny (n > 30 for each). Thus, if bacterial-mediated RNAi is having an effect in the touch neurons of wild-type animals, the effect is too small to generate a detectable phenotype. In contrast, in parallel experiments, lin-35 adults that had been fed with bacteria targeting mec-2, mec-3, mec-4, mec-9 and mec-18 throughout their larval development were touch insensitive, although the animals displayed the Mec phenotype with differences in penetrance and expressivity. Penetrance ranged from 47% (mec-9) to 83% (mec-2). Bacteria expressing mec-2, mec-3, and mec-4 dsRNA consistently gave a highly penetrant phenotype with strong expressivity (that is, the animals had a touch insensitivity similar to animals with null alleles). Bacteria making dsRNA for mec-12 produced a highly penetrant phenotype (63%) with intermediate strength (the animals responded to a few touches). mec-18 bacteria produced less consistent but easily detectable results; in some experiments the penetrance was high (60%) and expressivity strong, whereas in others the penetrance was lower (45%) and the expressivity intermediate. Bacteria producing mec-9 dsRNA gave the weakest positive results with penetrance of 47% and intermediate expressivity. These weaker effects seen with mec-9, mec-12 and mec-18 may be a consequence of the high expression of these genes in the touch neurons , which might overwhelm the RNAi machinery. Animals fed on bacteria targeting mec-8 or mec-10 were indistinguishable from those fed on bacteria for the gfp and sym-1 controls. Although negative RNAi results are difficult to interpret, genetic experiments  indicate that the amount of mec-8 activity produced in the embryo is sufficient for subsequent adult touch sensitivity, and elimination of mec-10 has only a slight effect on touch sensitivity (R O'Hagan, M Goodman, and MC, unpublished data).
These data indicate that neuronally expressed genes are effectively targeted by bacterial-mediated RNAi in the lin-35(n745) strain, thus providing a very useful tool to study gene function in these cells. These results also point to the expression and function of lin-35 in post-mitotic neurons.
A subset of synMuv B genes negatively regulate RNAi and somatic transgene silencing
A subset of synMuv B genes negatively regulate RNAi, somatic transgene silencing and expression of lag-2::gfp
Somatic transgene silencing†
Ectopic expression of lag-2::gfp ‡
lin-35 and lin-15B enhance somatic transgene silencing by an RNAi dependent mechanism
% Complete expression
Additional evidence suggests this subclassification of synMuv B genes is functionally relevant. Inactivation of a subset of synMuv B genes results in ectopic expression of a lag-2::gfp reporter gene . Strikingly, all of the synMuv B genes that we found to be negative regulators of the RNAi pathway and negative regulators of somatic transgene silencing also negatively regulate lag-2::gfp expression  (Table 4). This result suggests a similar synMuv B(R) pathway may regulate both the RNAi pathway and correct expression of this transgene, and supports the classification of synMuv B genes into at least two distinct functional subsets.
Genes required for RNAi can suppress the lineage defects of synMuvA;B strains
mes-4 and zfp-1 suppress the multivulval phenotype of lin-15A;B(n765) worms
100% (n = 300)
5% (n = 172)
46% (n = 96)
An increase in RNAi efficiency does not cause the lineage defects of synMuv B mutants
The precise molecular functions of the synMuv B(R) genes and of mes-4 and zfp-1 in vulva development and in RNAi are unknown; however many of these genes are predicted by sequence homology to regulate chromatin structure. One intriguing possibility is that a key function of the synMuv B(R) genes during vulva development may be to repress RNAi. The Muv phenotype might thus be due in part to alterations in RNAi-related processes. We investigated this in two complementary ways. Firstly, if the sole effect of synMuv B(R) genes on vulval development was through their effect on RNAi sensitivity, then other genes that similarly increase RNAi sensitivity should act as synMuv B genes. However, while targeting lin-35 by RNAi produces a strong Muv phenotype in a lin-15A mutant animal (as expected given its synMuv B activity), targeting eri-1 has no similar effect. Secondly, inactivation of the synMuv B(R) genes enhances RNAi and in the absence of synMuv A activity leads to multivulval development; to determine if these two functions were causally related, we asked whether inactivation of other genes that are essential for RNAi (rde-1, rde-4, rde-5, mut-7 or mut-16) suppresses the Muv phenotype of lin-15A;B(n765) - they do not. Hence, we find that genes that enhance RNAi do not all act as synMuv B genes and, conversely, that the RNAi machinery is not necessary for the synMuv phenotype. Thus, alterations in the efficacy of RNAi cannot alone account for the action of lin-35 in vulval development, although it may contribute to lin-35's role.
Wang et al.  suggest that the enhanced RNAi seen in synMuv B mutants may result from the misexpression of germline genes in somatic cells. Although this may contribute to the enhanced somatic RNAi seen in synMuv B strains, we found that lin-35(n745) animals also showed enhanced germline RNAi phenotypes (>50 genes gave strong sterility in lin-35(n745) but not in wild-type worms) (Additional data file 2). Although some of these sterile phenotypes may result from defects in somatic cells, a subset of these genes has been previously shown to function within the germline itself. In C. elegans, the Notch and MAP kinase pathways are both required within the germline for correct germline development [29, 30], and we found that four genes that function in these pathways also show strongly enhanced RNAi-induced sterility in lin-35(n745) worms (the genes glp-1, lag-1, let-60 and lin-45; Additional data file 3). Since the enhanced sterility seen with these genes must result from enhanced gene silencing within the germline itself, these data demonstrate that RNAi is also enhanced in the germline of lin-35(n745) worms, and that somatic misexpression of germline genes does not alone account for the enhanced RNAi seen in synMuv B mutants. We favour a model in which the synMuv B(R) genes and mes-4/zfp-1 act antagonistically to regulate the expression of a common set of target genes. These targets could include genes that are required for vulval development and genes required for RNAi, or the genes targeted by RNAi themselves. The antagonism may involve the direct repression of mes-4 and zfp-1 by the synMuv B(R) genes, or the antagonistic action of mes-4/zfp-1 and the synMuv B(R) genes on a common set of target genes (Figure 3). Alternatively, MES-4/ZFP-1 and the synMuv B(R) gene products may antagonise each other's functions by competing for a common set of co-factors.
We have found that lin-35 and a subset of synMuv B pathway genes negatively regulate RNAi in C. elegans, probably via a mechanism involving chromatin remodelling. The efficiency of RNAi is enhanced within both somatic and germline cells of lin-35 animals, demonstrating that misexpression of germline genes in somatic cells cannot alone account for the enhanced RNAi seen in this strain. lin-35(n745) is the most RNAi-sensitive single mutant strain identified to date and, therefore, should prove very useful for genome-wide RNAi screens. We note that the availability of five strains with varying RNAi-sensitivities (lin-35(n745) > lin-15B(n744) > eri-1(mg366) approximately = rrf-3(pk1426) > N2; Figure 1a) opens the possibility of studying an 'allelic series' of RNAi phenotypes for many genes (for example, when L1 wild-type or lin-15B(n744) worms are fed on bacteria targeting the gene ftt-2, they reach adulthood, at which point wild-type worms have a reduced brood size while the lin-15B(n744) worms are completely sterile; the RNAi phenotype is so severe in lin-35(n745) worms, however, that the L1 worms never reach adulthood, and instead show a completely penetrant larval growth arrest). We have also identified two genes (mes-4 and zfp-1) that are both required for RNAi and can suppress the vulval lineage defects resulting from inactivation of synMuv genes, suggesting a common mechanism for the action of synMuv B(R) genes in both of these processes. However, the increased efficiency of RNAi in synMuv B mutants does not alone explain the lineage defects of synMuv B strains.
Finally, it is possible that the human ortholog of LIN-35, p105Rb, may also negatively regulate RNAi, and its effect on the RNAi pathway may be important for its function as a tumour suppressor. In addition, inactivation of the human orthologs of mes-4 or zfp-1 may reverse some of the phenotypic consequences of mutations in p105Rb.
Materials and methods
RNAi screens by bacterial feeding
All of the RNAi feeding experiments described in this manuscript were performed in liquid culture by adding synchronised L1 stage worms, unless otherwise indicated. A total of 1,868 bacterial RNAi feeding strains from the Ahringer library  targeting 1,749 genes were tested with each worm strain. The vast majority of these genes are those annotated as 'signalling', 'chromatin', or 'transcription factors' in reference  (Additional data file 1); feeding approximately 75% of these bacterial strains gave no visible RNAi phenotypes in wild-type worms . Bacterial RNAi feeding strains were grown overnight at 37°C in 400 μl 2TY plus 100 μg/ml ampicillin, induced with 4 mM IPTG (isopropyl-beta-D-thiogalactopyranoside) at 37°C for 1 hour, and resuspended in 400 μl NGM(Nematode Growth Medium) plus 4 mM IPTG plus 100 μg/mlampicillin. Approximately 10 L1 stage worms were dispensed to each well of a 96-well flat bottomed tissue culture plate together with 40 μl of resuspended bacterial culture. The plates were incubated with shaking at 20°C for four days, and embryonic lethal, sterile, growth defect and post-embryonic phenotypes were scored on a dissecting microscope. All RNAi feeding experiments were performed in quadruplicate and the phenotypes observed in each strain were directly compared to those seen in N2 worms grown in parallel.
Transgene silencing assays
Worm strain JR667 expresses GFP specifically in the hypodermal seam cells from an integrated tandemly repeated array of the construct wIs51, which contains the scm::GFP reporter. Worm strain GR1401 expresses the same integrated GFP transgene, as well as a dsRNA that targets GFP mRNA for degradation, also specifically in the hypodermal seam cells of the worm . RNAi experiments were performed on six-well plates seeded with the indicated bacterial RNAi feeding strain (grown overnight as described above). Approximately 10 L1 stage worms were added to each well and incubated at 25°C for four days, unless otherwise indicated. The progeny worms were washed off the plates, paralysed in 100 mM levimasole and GFP expression was visualised using an Olympus IX81 microscope. Control experiments used a feeding strain that does not target any C. elegans gene (constructed using primers sjjY95B8A_84.g, defined in ). All other RNAi assays were performed exactly as described in .
Additional data files
The following additional data are available with the online version of this paper. Additional data file 1 provides the complete set of genes screened by RNAi feeding. Additional data file 2 lists genes with an enhanced RNAi phenotype in each of four RNAi hypersensitive strains, and in efl-1(se1). Additional data file 3 lists genes with nonviable RNAi phenotypes in lin-35(n745) that also have nonviable null phenotypes. Additional data file 4 lists genes from chromosome III tested for enhanced RNAi phenotypes in the strains lin-35(n745) and rrf-3(pk1426). Additional data file 5 lists genes from the start of chromosome III with an enhanced RNAi phenotype in lin-35(n745) compared to rrf-3(pk1426). Additional data file 6 is a figure showing that loss of dcr-1 does not suppress somatic transgene silencing resulting from inactivation of tam-1.
We thank the C. elegans Genetics Center for providing worm strains, Gary Ruvkun for providing worm strains and sharing prepublication data, and Irini Topalidou for assistance with the touch neuron screen. B.L. is supported by a Sanger Institute Postdoctoral Fellowship, C.C., J.T., A.F., and A.G.F. is supported by the Wellcome Trust, and M.C. is funded by NIH grant GC30997.
- Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE, Mello CC: Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature. 1998, 391: 806-811. 10.1038/35888.PubMedView ArticleGoogle Scholar
- Mello CC, Conte D: Revealing the world of RNA interference. Nature. 2004, 431: 338-342. 10.1038/nature02872.PubMedView ArticleGoogle Scholar
- Lehner B, Fraser AG, Sanderson CM: Technique review: how to use RNA interference. Brief Funct Genomic Proteomic. 2004, 3: 68-83. 10.1093/bfgp/3.1.68.PubMedView ArticleGoogle Scholar
- Kamath RS, Fraser AG, Dong Y, Poulin G, Durbin R, Gotta M, Kanapin A, Le Bot N, Moreno S, Sohrmann M, et al: Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature. 2003, 421: 231-237. 10.1038/nature01278.PubMedView ArticleGoogle Scholar
- Simmer F, Tijsterman M, Parrish S, Koushika SP, Nonet ML, Fire A, Ahringer J, Plasterk RH: Loss of the putative RNA-directed RNA polymerase RRF-3 makes C. elegans hypersensitive to RNAi. Curr Biol. 2002, 12: 1317-1319. 10.1016/S0960-9822(02)01041-2.PubMedView ArticleGoogle Scholar
- Kennedy S, Wang D, Ruvkun G: A conserved siRNA-degrading RNase negatively regulates RNA interference in C. elegans. Nature. 2004, 427: 645-649. 10.1038/nature02302.PubMedView ArticleGoogle Scholar
- Simmer F, Moorman C, van der Linden AM, Kuijk E, van den Berghe PV, Kamath RS, Fraser AG, Ahringer J, Plasterk RH: Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals novel gene functions. PLoS Biol. 2003, 1: E12-10.1371/journal.pbio.0000012.PubMedPubMed CentralView ArticleGoogle Scholar
- Lu X, Horvitz HR: lin-35 and lin-53, two genes that antagonize a C. elegans Ras pathway, encode proteins similar to Rb and its binding protein RbAp48. Cell. 1998, 95: 981-991. 10.1016/S0092-8674(00)81722-5.PubMedView ArticleGoogle Scholar
- Kim JK, Gabel HW, Kamath RS, Tewari M, Pasquinelli A, Rual JF, Kennedy S, Dybbs M, Bertin N, Kaplan JM, et al: Functional genomic analysis of RNA interference in C. elegans. Science. 2005, 308: 1164-1167. 10.1126/science.1109267.PubMedView ArticleGoogle Scholar
- Wang D, Kennedy S, Conte D, Kim JK, Gabel HW, Kamath RS, Mello CC, Ruvkun G: Somatic misexpression of germline P granules and enhanced RNA interference in retinoblastoma pathway mutants. Nature. 2005, 436: 593-597. 10.1038/nature04010.PubMedView ArticleGoogle Scholar
- Frolov MV, Dyson NJ: Molecular mechanisms of E2F-dependent activation and pRB-mediated repression. J Cell Sci. 2004, 117: 2173-2181. 10.1242/jcs.01227.PubMedView ArticleGoogle Scholar
- Ceol CJ, Horvitz HR: dpl-1 DP and efl-1 E2F act with lin-35 Rb to antagonize Ras signaling in C. elegans vulval development. Mol Cell. 2001, 7: 461-473. 10.1016/S1097-2765(01)00194-0.PubMedView ArticleGoogle Scholar
- Page BD, Guedes S, Waring D, Priess JR: The C. elegans E2F- and DP-related proteins are required for embryonic asymmetry and negatively regulate Ras/MAPK signaling. Mol Cell. 2001, 7: 451-460. 10.1016/S1097-2765(01)00193-9.PubMedView ArticleGoogle Scholar
- Boxem M, van den Heuvel S: C. elegans class B synthetic multivulva genes act in G(1) regulation. Curr Biol. 2002, 12: 906-911. 10.1016/S0960-9822(02)00844-8.PubMedView ArticleGoogle Scholar
- Fay DS, Qiu X, Large E, Smith CP, Mango S, Johanson BL: The coordinate regulation of pharyngeal development in C. elegans by lin-35/Rb, pha-1, and ubc-18. Dev Biol. 2004, 271: 11-25. 10.1016/j.ydbio.2004.03.022.PubMedView ArticleGoogle Scholar
- Moghal N, Sternberg PW: A component of the transcriptional mediator complex inhibits RAS-dependent vulval fate specification in C. elegans. Development. 2003, 130: 57-69. 10.1242/dev.00189.PubMedView ArticleGoogle Scholar
- Chalfie M, Sulston J: Developmental genetics of the mechanosensory neurons of Caenorhabditis elegans. Dev Biol. 1981, 82: 358-370. 10.1016/0012-1606(81)90459-0.PubMedView ArticleGoogle Scholar
- Chalfie M, Au M: Genetic control of differentiation of the Caenorhabditis elegans touch receptor neurons. Science. 1989, 243: 1027-1033.PubMedView ArticleGoogle Scholar
- Zhang Y, Chalfie M: MTD-1, a touch-cell-specific membrane protein with a subtle effect on touch sensitivity. Mech Dev. 2002, 119: 3-7. 10.1016/S0925-4773(02)00293-9.PubMedView ArticleGoogle Scholar
- Zhang Y, Ma C, Delohery T, Nasipak B, Foat BC, Bounoutas A, Bussemaker HJ, Kim SK, Chalfie M: Identification of genes expressed in C. elegans touch receptor neurons. Nature. 2002, 418: 331-335. 10.1038/nature00891.PubMedView ArticleGoogle Scholar
- Lipsick JS: synMuv verite - Myb comes into focus. Genes Dev. 2004, 18: 2837-2844. 10.1101/gad.1274804.PubMedView ArticleGoogle Scholar
- Lewis PW, Beall EL, Fleischer TC, Georlette D, Link AJ, Botchan MR: Identification of a Drosophila Myb-E2F2/RBF transcriptional repressor complex. Genes Dev. 2004, 18: 2929-2940. 10.1101/gad.1255204.PubMedPubMed CentralView ArticleGoogle Scholar
- Korenjak M, Taylor-Harding B, Binne UK, Satterlee JS, Stevaux O, Aasland R, White-Cooper H, Dyson N, Brehm A: Native E2F/RBF complexes contain Myb-interacting proteins and repress transcription of developmentally controlled E2F target genes. Cell. 2004, 119: 181-193. 10.1016/j.cell.2004.09.034.PubMedView ArticleGoogle Scholar
- Sieburth D, Ch'ng Q, Dybbs M, Tavazoie M, Kennedy S, Wang D, Dupuy D, Rual JF, Hill DE, Vidal M, et al: Systematic analysis of genes required for synapse structure and function. Nature. 2005, 436: 510-517. 10.1038/nature03809.PubMedView ArticleGoogle Scholar
- Hsieh J, Liu J, Kostas SA, Chang C, Sternberg PW, Fire A: The RING finger/B-box factor TAM-1 and a retinoblastoma-like protein LIN-35 modulate context-dependent gene silencing in Caenorhabditis elegans. Genes Dev. 1999, 13: 2958-2970. 10.1101/gad.13.22.2958.PubMedPubMed CentralView ArticleGoogle Scholar
- Poulin G, Dong Y, Fraser AG, Hopper NA, Ahringer J: Chromatin regulation and sumoylation in the inhibition of Ras-induced vulval development in Caenorhabditis elegans. EMBO J. 2005, 24: 2613-23. 10.1038/sj.emboj.7600726.PubMedPubMed CentralView ArticleGoogle Scholar
- Dudley NR, Labbe JC, Goldstein B: Using RNA interference to identify genes required for RNA interference. Proc Natl Acad Sci USA. 2002, 99: 4191-4196. 10.1073/pnas.062605199.PubMedPubMed CentralView ArticleGoogle Scholar
- Chaplin T, Ayton P, Bernard OA, Saha V, Della Valle V, Hillion J, Gregorini A, Lillington D, Berger R, Young BD: A novel class of zinc finger/leucine zipper genes identified from the molecular cloning of the t(10;11) translocation in acute leukemia. Blood. 1995, 85: 1435-1441.PubMedGoogle Scholar
- Church DL, Guan KL, Lambie EJ: Three genes of the MAP kinase cascade, mek-2, mpk-1/sur-1 and let-60 ras, are required for meiotic cell cycle progression in Caenorhabditis elegans. Development. 1995, 121: 2525-2535.PubMedGoogle Scholar
- Austin J, Kimble J: glp-1 is required in the germ line for regulation of the decision between mitosis and meiosis in C. elegans. Cell. 1987, 51: 589-599. 10.1016/0092-8674(87)90128-0.PubMedView ArticleGoogle Scholar
- Wormbase. [http://www.wormbase.org]
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