CTCF and cohesin promote focal detachment of DNA from the nuclear lamina
Genome Biology volume 23, Article number: 185 (2022)
Lamina-associated domains (LADs) are large genomic regions that are positioned at the nuclear lamina. It has remained largely unclear what drives the positioning and demarcation of LADs. Because the insulator protein CTCF is enriched at LAD borders, it was postulated that CTCF binding could position some LAD boundaries, possibly through its function in stalling cohesin and hence preventing cohesin invading into the LAD. To test this, we mapped genome–nuclear lamina interactions in mouse embryonic stem cells after rapid depletion of CTCF and other perturbations of cohesin dynamics.
CTCF and cohesin contribute to a sharp transition in lamina interactions at LAD borders, while LADs are maintained after depletion of these proteins, also at borders marked by CTCF. CTCF and cohesin may thus reinforce LAD borders, but do not position these. CTCF binding sites within LADs are locally detached from the lamina and enriched for accessible DNA and active histone modifications. Remarkably, despite lamina positioning being strongly correlated with genome inactivity, this DNA remains accessible after the local detachment is lost following CTCF depletion. At a chromosomal scale, cohesin depletion and cohesin stabilization by depletion of the unloading factor WAPL quantitatively affect lamina interactions, indicative of perturbed chromosomal positioning in the nucleus. Finally, while H3K27me3 is locally enriched at CTCF-marked LAD borders, we find no evidence for an interplay between CTCF and H3K27me3 on lamina interactions.
These findings illustrate that CTCF and cohesin are not primary determinants of LAD patterns. Rather, these proteins locally modulate NL interactions.
The nuclear lamina (NL) is positioned near the inner nuclear membrane and consists of a protein meshwork of A- and B-type lamins and a variety of other proteins. Peripherally positioned chromatin interacts with the NL and comprises approximately a thousand large genomic domains that are up to 10 Mb in size . These lamina-associated domains (LADs) are strongly depleted for active genes and are thought to provide a backbone for genome organization. LADs are highly conserved between cell types and species, but the mechanisms that underlie genome positioning at the NL remain poorly understood (reviewed in [2,3,4]).
LADs are strongly enriched for features of heterochromatin. Besides having a low gene density and typically low gene expression levels, LADs replicate late in S-phase, are visible by electron microscopy as densely packed chromatin, and are enriched for the histone modifications H3K9me2 and H3K9me3 [1, 5,6,7,8,9]. These heterochromatin characteristics are important because gene activation, chromatin decondensation, and H3K9me2/3 perturbation all result in a loss of DNA–NL interactions [7, 10,11,12,13,14]. LAD interactions with the NL are mediated by various proteins in a redundant manner, including but probably not limited to Lamin B receptor (LBR), Lamin A, and CEC-4 (the latter only in C. elegans) [13, 15]. Differences in NL interactions between cell types are likely caused by variable epigenetic landscapes and protein compositions of the NL.
One particular aspect of LADs that remains poorly understood is the sharp transition in NL interactions at LAD borders, which suggests the existence of specific mechanisms to define these borders. LAD borders are enriched for active promoters, CpG islands (often at active promoters), and binding of the insulator protein CTCF . While there is increasing evidence that gene activity results in local detachment from the NL [11, 14], which could explain a sharp transition at some borders, the role of CTCF on LAD border positioning is still unclear. Because few borders are marked by both CTCF and active promoters, it was postulated that CTCF may independently demarcate at least a subset of LAD borders [1, 12]. In support of this hypothesis, a deletion of a ~30-kb LAD border region at the Tcrb locus resulted in decreased NL interactions up to 100 kb inside the LAD, and this behavior was almost completely recapitulated by deletion of a ~4.5-kb region that included all three CTCF binding sites at this border . However, a genome-wide view of a possible causal role of CTCF in LAD border formation is still lacking.
CTCF is a broadly expressed and essential zinc-finger protein that interacts with the ring-shaped cohesin complex on the genome [17, 18]. Once loaded on DNA, cohesin is thought to establish long-range DNA contacts through a processive increase in chromatin loop size, a process known as extrusion ( [19, 20], reviewed in ). Extrusion is halted by the interaction of the SA1/2-RAD21 interface of the cohesin complex with the N-terminal regions of CTCF [22, 23]. Cohesin is actively loaded by the NIPBL/MAU2 complex and unloaded by the cohesin release factor WAPL to maintain a continuous cycle of loop extrusion [24, 25]. Together with other factors, such as cell-type-specific transcription factors, cohesin mediates a continuous cycle of loop extrusion to preserve distal gene regulation . Remarkably, besides having extended DNA loops, WAPL-knockout cells also have somewhat fragmented LADs . However, the mechanism by which WAPL loss affects NL interactions remains to be elucidated.
In addition to the genomic features discussed above, in some cell types, H3K27me3 is also enriched near LAD borders [1, 12]. H3K27me3 and CTCF could be involved in LAD demarcation together, given that both were reported to be involved in the peripheral positioning of ectopically integrated LAD fragments . However, the role of H3K27me3 may be cell-type specific, as this mark is not correlated with NL interactions in all cell types [10, 27]. Additionally, H3K27me3 is associated with dynamic LADs during the cell cycle , oncogene-induced senescence , and strain-induced genome repositioning .
Two recent developments now allowed us to investigate the interplay between LAD borders and CTCF and cohesin. First, the fusion of endogenous proteins with auxin-inducible degron (AID) tags mediates rapid and near-complete protein depletion, which is particularly useful for essential proteins or complexes such as CTCF and cohesin [31, 32]. Second, the application of proteinA-DamID (pA-DamID) results in the mapping of genome–NL interactions with a high temporal resolution, which is required to study the effects of rapid protein depletion . Here, using a combination of these two tools in mouse embryonic stem cells (mESCs), we show that CTCF and cohesin mediate focal detachment from the NL, but have limited effects on LAD border positioning. On a chromosomal scale, perturbation of cohesin dynamics induces quantitative changes of NL interactions, which signifies that cohesin affects the radial positioning of the genome. Finally, pharmacological depletion of H3K27me3 does not affect NL interactions in mESCs, even in combination with CTCF depletion.
LAD borders are enriched for CTCF binding in multiple cell lines
We first set out to explore the correlation between CTCF binding (as detected by ChIP-seq) and LAD borders in more detail by comparing these in two mouse and four human cell lines with available NL interaction maps (obtained by DamID). CTCF binding is consistently depleted within LADs and locally enriched near LAD borders, peaking 10 kb outside LADs (Fig. 1A). CTCF is enriched at cell-type-specific borders and at LAD borders shared between cell types (Additional file 1: Fig. S1A), although shared borders often have overall slightly elevated CTCF density. Thus, CTCF enrichment at LAD borders is not specific to either stable or variable borders.
CTCF binds the genome in an oriented manner due to a non-palindromic motif. Loops primarily occur between two convergent CTCF motifs [45,46,47]. To test whether LAD borders are enriched for a certain orientation, directionality of CTCF binding sites was assigned based on the orientation of its motif. We observe that LAD borders are enriched for CTCF binding sites with both motif orientations (Fig. 1A) and therefore are likely involved in DNA looping towards (inwards) and away from (outwards) LADs.
CTCF is thus positioned at a subset of LAD borders, which raises the question whether these borders are different from LAD borders without CTCF. We classified all LAD borders into two categories based on nearby CTCF binding (positioned within 20 kb outside borders, overlapping with the observed enrichment), which results in 42–62% being classified as “CTCF borders” for the different cell types (Fig. 1B). In accordance with previous observations , LAD borders are also enriched for transcription, indicated by a local enrichment of transcription start sites (TSS) and transcription end sites (TES) for genes transcribing from and towards the LAD, respectively (Additional file 1: Fig. S1B). Active genes are present at LAD borders with and without CTCF binding (Fig. 1B). Because transcription is known to induce NL detachment locally , we excluded borders near active genes from further analyses unless otherwise indicated, to avoid confounding effects from transcription on NL interactions and LAD border positioning.
Average profiles of NL interactions, CTCF, and cohesin binding and publicly available epigenetic data [26, 40,41,42,43,44] at mESC LAD borders reveal that borders with CTCF generally show a somewhat sharper transition between LAD and inter-LAD (iLAD), for example in NL interactions, ATAC-seq and H3K4me1 (Fig. 1C). As expected, LAD borders with CTCF binding are locally enriched for RAD21 binding (a subunit of the cohesin complex). Finally, LAD borders with CTCF binding are locally enriched for H3K27me3 and depleted for H3K9me2. All of these enrichments are irrespective of the orientation of the CTCF motif (Additional file 1: Fig. S1C-D) and are mostly conserved in human H1 and HCT116 cells, although H3K27me3 enrichment in HCT116 cells is not limited to LAD borders (Additional file 1: Fig. S1E-F). Together, these data show that CTCF enrichment at LAD borders is conserved between cell types and that CTCF binding correlates with a different epigenetic makeup, and raise the question whether CTCF is involved in genome positioning at the NL.
The majority of NL interactions are maintained upon depletion of CTCF
To test whether CTCF is directly involved in the demarcation of LAD borders, we used a mESC line with CTCF fused to an auxin-inducible degron (AID) tag . Addition of auxin (IAA) results in rapid and near-complete protein depletion within several hours, allowing us to distinguish direct effects of CTCF depletion from secondary effects such as perturbed growth and cell differentiation . We verified that IAA addition resulted in CTCF depletion (Additional file 1: Fig. S2A). Because the AID tag already resulted in a substantial decrease of CTCF levels even in the absence of IAA (Additional file 1: Fig. S2A), we used the parental clone (PT) expressing only OsTir1 as an additional control in these experiments.
We then used our recently developed pA-DamID procedure to map NL interactions at multiple time points after addition of IAA. Compared to conventional DamID, pA-DamID can map NL interactions with an increased temporal resolution . The pA-DamID tracks generated in the parental line are very similar to DamID tracks in wildtype mESCs except for a reduced dynamic range (Additional file 1: Fig. S2B-C) and are highly reproducible between biological replicates (Additional file 1: Fig. S2D-E) and LAD borders called from pA-DamID tracks are equally enriched for CTCF binding (Additional file 1: Fig. S2F-G). Thus, the tracks generated with pA-DamID are in accordance with the DamID tracks and can be used to generate “snapshots” of NL interactions after protein depletion. To account for differences in dynamic range present between individual replicates and conditions, we converted log2 ratios of LaminB1/Dam-only to z-scores (Additional file 1: Fig. S2H). On average, a differential z-score of 1 corresponds to a 2.1-fold difference in the linear pA-DamID score (Additional file 1: Fig. S2I). The z-scores were averaged between biological replicates for downstream analyses .
NL interaction patterns are largely maintained after depletion of CTCF, but there are some small differences that gradually increase over time (Fig. 1D). As prolonged CTCF depletion can induce secondary effects in gene regulation and genome organization, we focused the following analyses on the early time points (6 h and 24 h). We observed no change in the cell cycle distribution after 6 h of CTCF depletion, but after 24 h, there is a weak depletion of S phase cells (Additional file 1: Fig. S3). The average profile of NL interactions across all combined LAD borders does not reveal large differences between CTCF-marked and CTCF-unmarked LAD borders in NL interactions (Fig. 1E). However, compared to unmarked borders, after CTCF depletion, we do observe a small but noticeable gain in NL interactions in the iLAD flanking CTCF-marked LAD borders (Fig. 1E, red arrows), precisely coinciding with the location of the CTCF binding sites (Fig. 1A). This change is already present after 6 h, suggesting that this is a direct effect of CTCF depletion. Quantification at individual LAD borders suggests a global increase at most borders rather than a strong effect at a small subset of borders (Fig. 1F). Approximately 25% of the CTCF-marked LAD borders shift ~20 kb into the iLAD after CTCF depletion (Fig. 1G).
Next, we performed similar analyses with different LAD border segmentations. First, we obtained comparable results for both CTCF orientations, suggesting that the observations are not caused by a directional loop extrusion process originating in either the LAD or iLAD (Additional file 1: Fig. S4A-C). Second, at LAD borders near active genes, CTCF depletion still induces a local gain at the CTCF binding site but does not affect the positioning of CTCF-marked borders (Additional file 1: Fig. S4D-F). It is thus likely that the active genes maintain the positioning of these borders. Third, to test if LAD borders with multiple CTCF binding sites are more dependent on CTCF, we segmented borders based on the amount of CTCF peaks within 20 kb. Indeed, we find that the number of CTCF binding sites positively correlates with the local gain outside of the LAD border (Fig. 1H) and the shift in LAD border positioning (Additional file 1: Fig. S4G-I). These data indicate that CTCF contributes to the sharpening of LAD borders, by counteracting DNA–NL contacts just outside of the borders, and thereby assists in LAD border positioning. However, we observe no dramatic change in global LAD patterns, indicating that CTCF is primarily involved in the fine-tuning of NL interactions.
NL detachment at CTCF LAD borders is mediated by CTCF and cohesin
The looping function of CTCF is tightly linked to cohesin, a ring-shaped protein complex involved in the formation of long-range DNA contacts. Cohesin is stalled at CTCF binding sites in the genome and in turn is released from DNA by WAPL (reviewed in ). We observed that LAD borders with CTCF binding are enriched for RAD21 (Fig. 1C). Moreover, we find that CTCF-marked borders coincide more frequently with loop anchors than borders without CTCF  (Fig. 2A). These data together with the observation that WAPL knockout cells have fragmented LADs  suggest that cohesin may also be involved in LAD border organization. To directly test this, we mapped NL interactions in mESCs after rapid cohesin depletion (AID-tagged RAD21) , cohesin stabilization (AID-tagged WAPL) , and a combination of CTCF depletion and cohesin stabilization (AID-tagged CTCF and WAPL)  (Additional file 1: Fig. S2A). Similar to CTCF, AID-tagging of WAPL already leads to a substantial protein loss prior to addition of IAA, but WAPL is completely lost 6 h after IAA addition (Additional file 1: Fig. S2A). In accordance with a previous report, RAD21 depletion induces a strong enrichment of G2 cells  (Additional file 1: Fig. S3). The cell cycle distribution is virtually unaffected by WAPL depletion  or by a combined CTCF/WAPL depletion (Additional file 1: Fig. S3).
Similar to CTCF, these depletion experiments do not grossly alter the global patterns of NL interactions, although again various changes can be seen (Fig. 2B). Intriguingly, the changes differ strongly depending on the protein depleted. For example, RAD21 depletion results in changes that are sometimes opposite of those after CTCF depletion (orange and blue arrows). WAPL depletion rapidly fragments some LADs (red arrows), similar to a prolonged loss of WAPL . A double depletion of WAPL and CTCF prevents LAD fragmentation, but does result in perturbed NL interactions for some LADs (pink arrows). All these changes that can be seen by visual inspection are likely caused by a combination of various mechanisms that we will discuss individually below.
Average profiles of NL interactions at LAD borders show that RAD21 depletion results in a small decrease in NL interactions around LAD borders marked by CTCF, both outside and inside the LAD (Fig. 2C, left panels). This likely reflects a global change in NL interactions rather than a local effect of cohesin at the LAD border. We do not observe a specific shift of CTCF-marked LAD borders compared to unmarked borders (Fig. 2E). A slight loss of the local dip in the average NL interaction profile at the position of CTCF binding sites (Fig. 2C, red arrows, Fig. 2D) seems to occur after RAD21 depletion, but this is largely masked by the overall decrease in NL interactions at CTCF-marked LAD borders.
More pronounced effects are seen after WAPL depletion (Fig. 2C, middle panels). The local dip in NL interactions just outside of CTCF-marked borders becomes much more pronounced (Fig. 2D), while these borders shift on average about 10–50 kb inwards (Fig. 2E). Remarkably, double depletion of WAPL and CTCF almost completely reverts this effect, underscoring that CTCF is required for cohesin-mediated local detachment (Fig. 2D, right panels). Under this double depletion condition, approximately 20% of the LAD borders with CTCF binding sites tend to shift slightly (~10 kb) into the iLAD (Fig. 2E).
Similar results are obtained for LAD borders bound by CTCF in either orientation (Additional file 1: Fig. S5A-B). We note that all observed changes in pA-DamID signals discussed above are caused by a change in LaminB1 reads rather than Dam control reads, illustrating that the observed differences are not due to changes in DNA accessibility (Additional file 1: Fig. S5C-D). We conclude that CTCF and cohesin can promote local detachment of DNA from the NL near LAD borders, which may help to reinforce these borders.
Cohesin-mediated local detachment of CTCF-bound chromatin from the NL inside LADs
The data presented so far indicate that chromatin bound by CTCF can locally detach DNA from the NL at LAD borders. This raises the question whether this is specific for LAD borders, or also holds true for CTCF binding sites within LADs. Small euchromatin islands in large heterochromatin domains (typically overlapping with LADs ) are often devoid of a TSS and enriched for active marks (DNA accessibility and H3K9ac) and binding of CTCF . However, it is unclear whether CTCF is required to locally escape from the NL at these sites.
Consistent with previous work [11, 14, 52, 53], actively transcribed genes inside LADs locally detach from the NL in mESCs (Fig. 3A). Again, to avoid confounding effects from this expression-mediated detachment, we focused on CTCF binding sites that are positioned at least 100 kb away from active genes. We find that CTCF sites inside LADs moderately detach from the NL compared to the surrounding regions (Fig. 3A). Note that these detachments are not strong enough to cause our LAD-calling algorithm to mark these sites as iLADs.
Similar to LAD borders, CTCF binding in LADs is correlated with features of open chromatin (ATAC, H3K4me1, and H3K27ac), although to a much lesser extent than active genes. CTCF occupancy also coincides with a local decrease and increase of H3K9me2 and H3K27me3, respectively (Fig. 3B). These data are in accordance with our observations at LAD borders.
Average NL interaction profiles across active genes, CTCF sites, and random LAD locations show that CTCF and cohesin are required for local detachment of CTCF sites within LADs (Fig. 3C). This effect appears to be dose-dependent, as this detachment is slightly more pronounced in the PT line (with wild-type protein levels) compared to the clone with AID-tagged CTCF in the absence of IAA (which has a partial protein depletion) (Additional file 1: Fig. S6A). The changes in NL contacts are not observed upon treatment of PT cells with IAA and hence are not the result of an off-target effect of IAA (Additional file 1: Fig. S6B). Moreover, there is no change in NL interaction of active genes after any protein depletion (Fig. 3C), underscoring that the effects are specific for CTCF binding sites. WAPL depletion increases this local detachment of CTCF sites in a CTCF-dependent (Fig. 3C) and dose-dependent manner (Additional file 1: Fig. S6A). Together, these data indicate that CTCF- and cohesin-mediated detachment from the NL is not limited to LAD borders and that this involves a mechanism that is distinct from transcription-mediated detachment.
Next, we tried to better understand the local detachment of CTCF binding sites from the NL. We hypothesized that CTCF and cohesin together mediate long-range DNA interactions of CTCF binding sites with non-LAD regions, which then compete with NL contacts. Both within and outside LADs, CTCF binding sites frequently overlap with loop anchors (Fig. 3D). Loops are often formed within iLADs and within LADs, with limited cross-talk (Fig. 3E). We calculated a detachment score for every CTCF binding site in LADs, defined as the difference in NL interactions between the CTCF binding site and its flanking 100-kb regions (Fig. 3F). In accordance with our hypothesis, local detachment is correlated with the presence of a loop anchor (Fig. 3G), in particular when the looping partner is positioned outside of a LAD (Fig. 3G).
Chromatin accessibility at CTCF binding sites is independent of NL detachment
Local NL detachment at CTCF binding sites with active chromatin features is thus dependent on CTCF and cohesin. This raises the question whether the detachment is required for the active chromatin features. To address this question, we generated ATAC-seq data to determine DNA accessibility  — one of the features enriched at CTCF binding sites — after depletion of CTCF. The results show that CTCF-mediated detachment is not required to maintain DNA accessibility (Fig. 3H). We obtained similar results after depletion of RAD21 (Fig. 3H). Combined, we conclude that CTCF-bound chromatin locally detaches from the NL, but that this is not required to maintain an accessible chromatin state.
Architectural proteins control genome-wide patterns of NL interactions
Up to this point, we analyzed local changes of NL interactions at CTCF binding sites. Next, we searched for more global effects of CTCF and cohesin on NL interactions, for example involving entire LADs or large chromosomal regions. Towards this goal, we calculated mean pA-DamID scores on a consensus set of LADs across all conditions and then determined the changes in scores in each depletion experiment (see the “Methods” section, Additional file 1: Fig. S7). We observed the smallest changes in LAD scores upon CTCF depletion and the biggest changes upon the double depletion of WAPL and CTCF (Fig. 4A). To quantify the fraction of affected LADs, we counted LADs that changed beyond the PT distribution. This revealed that up to 25% of the LADs are affected by the CTCF/WAPL depletion (Fig. 4B). Consistent with visual observations of the chromosome profiles illustrated previously (Fig. 2C), LAD-wide changes in NL interactions are mostly uncorrelated between the depleted proteins, except for WAPL and the double combination of CTCF and WAPL (Fig. 4C).
We then compared LAD-wide changes in NL interactions with several features that describe LADs and their chromosomal context: LAD size, density of CTCF binding and active genes, mean signal for the repressive histone modifications H3K27me3 and H3K9me2, local LAD density, distance to centromeres and telomeres, and chromosome size. Except for chromosomal positioning, most of these features are largely independent (Additional file 1: Fig. S8). This analysis revealed multiple significant correlations that strongly differ depending on the depleted proteins (Fig. 4D). For example, depletion of CTCF preferentially results in decreased NL interactions of small LADs, while loss of WAPL preferentially reduces NL interactions of large LADs (Fig. 4E, top panels). Furthermore, the effects of RAD21 and WAPL on LAD-wide NL interactions both depend on the chromosomal position (i.e., distance to telomere and centromere), but in opposite orientation. This effect appears roughly linear with distance to the telomeres and is present across all chromosomes (Fig. 4E, bottom panels). Chromatin nearby telomeres is enriched at the NL in early G1 cells and slowly loses interactions with the NL in interphase . Possibly stabilized cohesin somehow prevents this normal maturation of NL interactions. It is unlikely that these observations are a direct consequence of a perturbed cell cycle, because the latter is only strongly affected by the RAD21 depletion (Additional file 1: Fig. S3).
We also found various correlations with local LAD density, CTCF density, and gene density (Fig. 4D). While these intriguing global links are currently difficult to interpret mechanistically, they illustrate that the CTCF/cohesin machinery affects NL interactions not only locally, but also at the scale of entire chromosomes. Interestingly, we found only few and very modest significant correlations with H3K9me2 and H3K27me3, suggesting that the effects of perturbed cohesin dynamics are largely independent of these histone modifications.
Genome-wide changes in NL interactions are not mediated by transcription
Altered genome positioning at the NL is strongly correlated with transcriptional changes during differentiation . This is particularly relevant here, as depletions of WAPL and RAD21 induce a differentiation-like phenotype . To test whether the genome-wide effects on NL interactions are mediated by transcriptional changes, we analyzed RNA-seq data after all protein depletion experiments.
Principal component analysis (PCA) (Additional file 1: Fig. S9A) and differential expression analysis (Fig. 5A, B) indicate that all four protein depletions have a partial overlap in their effect on gene expression, although the effects of RAD21 depletion and the double CTCF/WAPL depletion are more pronounced than those of individual depletion of CTCF or WAPL. We found that genes known to be differentially expressed genes during mESC differentiation into neural precursor cells  are significantly affected after all depletions (Additional file 1: Fig. S9B-C). This implies that both CTCF and dynamic cohesin are required to maintain a normal gene regulation program and prevent mESC differentiation . However, we found that LADs with up- and downregulated genes do not consistently exhibit decreased and increased NL interactions, respectively (Fig. 5C). This is particularly true for LADs with early changes in gene expression (after 24 h of RAD21 and CTCF/WAPL depletions). We obtain similar results with nascent transcription data following WAPL depletion (Additional file 1: Fig. S9D) . We conclude that the genome-wide changes in NL interactions are generally not mediated by transcriptional changes induced by the protein depletions.
We also asked whether LAD genes are specifically affected following cohesin perturbations. Most LAD genes are not expressed and their promoters are also not expressed when placed in a neutral chromatin environment . Such genes may simply lack critical transcription factors and are thus unlikely to be upregulated by changes in chromatin architecture alone. To remove such unresponsive genes, we required LAD genes to have a minimum expression level in at least one of the conditions and selected a matching set of iLAD genes with a similar distribution of expression levels as these LAD genes (Additional file 1: Fig. S9E). For all depletion experiments, differentially expressed genes are not significantly enriched in LADs (Additional file 1: Fig. S9F). We conclude that CTCF and cohesin can affect gene regulation, but not preferentially in LADs.
NL interactions are not dependent on H3K27me3
We find that H3K27me3 is locally enriched near CTCF binding sites positioned at LAD borders and within LADs. This is of interest, because in human fibroblasts CTCF and EZH2 (methyltransferase of H3K27me3) were both reported to be involved in the peripheral positioning of LAD fragments . Furthermore, CTCF binding sites that overlap with H3K27me3 domains show a local increase in H3K27me3 signal after CTCF depletion in mESCs cultured in serum conditions . Combined, these data may signify that a loss of CTCF is compensated by locally increased H3K27me3 and that a double depletion of CTCF and H3K27me3 is required to perturb LAD border positioning.
We first generated calibrated ChIP-seq data for H3K27me3 before and after CTCF depletion in mESCs cultured in 2i conditions. Alignment at LAD borders indicates that the enrichment of H3K27me3 is not affected by CTCF depletion; if anything, it becomes more pronounced (Fig. 6A). Next, to test the hypothesis that H3K27me3 and CTCF redundantly position LAD borders, we used two H3K27me3 methyltransferase inhibitors (GSK126 and EED226) that induce a near-complete loss of this mark (Additional file 1: Fig. S10B-C), and profiled NL interactions with and without CTCF depletion (Fig. 6B). H3K27me3 inhibition by itself does not affect global genome positioning at the NL (Additional file 1: Fig. S10D) or specifically at LAD borders with CTCF binding (Fig. 6C). Furthermore, a double depletion of H3K27me3 and CTCF has no additional effects besides those previously observed upon CTCF depletion alone (Fig. 6C–E).
We therefore conclude that there is no interplay between CTCF and H3K27me3 in genome positioning at the NL in mESCs. This result also suggests that the genome-wide correlation between NL interactions and H3K27me3 observed after CTCF depletion is not caused by the H3K27me3 marks (Fig. 4D), further supporting that the effects of perturbed cohesin dynamics on NL interactions are independent from histone modifications.
Combinations of chromosome conformation capture methods and rapid protein depletion experiments revealed how CTCF and cohesin organize the genome in self-associating domains [24, 25, 32, 57, 58]. However, it has remained mostly unclear how these processes affect nuclear positioning of the genome relative to the nuclear lamina. Here, we show that CTCF and cohesin reinforce LAD border positioning by mediating local detachment from the NL and affect genome positioning at the NL quantitatively. The observed effects of CTCF and cohesin on NL interactions appear to be relatively modest. Because the chromatin makeup of mESCs is quite unique compared to differentiated cells, the effects may be more or less pronounced in other cell types. It will be interesting to explore this in the future, using the combination of pA-DamID and degron-tagged proteins as described here.
CTCF mediates local detachment from the NL
We find CTCF and cohesin enrichment at LAD borders in all tested mouse and human cell lines, indicating that this is a conserved feature of LADs [1, 12]. Our data in mESCs indicate that these proteins are not required to maintain LAD border positioning, but rather contribute to a sharper transition in NL interactions. This result complements previous work indicating that CTCF loss does not trigger spreading of H3K27me3 heterochromatin . CTCF binding may still be a boundary for other types of heterochromatin, as H3K9me2 spreading was observed following CTCF depletion for a number of genes , but further studies are required to validate this. Furthermore, it remains to be elucidated whether there is a functional impact of a sharper transition in NL interactions.
Small euchromatin regions inside heterochromatin blocks can be divided in promoter elements and regulatory elements based on genomic features and chromatin marks, both of which are enriched for CTCF binding sites . This is in accordance with our results, which indicate that isolated CTCF sites in LADs are enriched for active marks and locally detach from the NL. We show that NL detachment at these sites requires both CTCF and cohesin. Thus, besides transcription [11, 14, 52, 53], DNA looping mediated by CTCF and cohesin can contribute to the local dissociation of DNA from the NL. Upon cohesin stabilization, this CTCF-mediated detachment can fully fracture LAD domains and thus establish new LAD borders . Our data reveal a stronger detachment for CTCF sites that loop with iLAD regions. This result suggests that extrusion can pass LAD borders and upon loop stabilization mediate focal dissociation of specific LAD sequences towards the nuclear interior.
Intriguingly, CTCF and cohesin are dispensable for DNA accessibility at CTCF binding sites in LADs. This is of interest, as artificial decondensation of LADs can induce repositioning from the NL, even without transcriptional activity [7, 11, 60]. While DNA accessibility does not directly mirror chromatin decondensation, it may indicate that this repositioning also depends on CTCF and cohesin. Alternatively, these observations could be explained by a mechanism independent from transcription and CTCF-mediated DNA looping to detach from the NL.
Cohesin dynamics affect genome-wide patterns of NL interactions
Besides local effects at LAD borders and CTCF binding sites, CTCF and cohesin affect the genome-wide pattern of NL positioning quantitatively. We observe the largest effects upon depletion of WAPL and combined depletion of CTCF and WAPL. While these genome-wide changes are difficult to interpret mechanistically, they reveal intriguing correlations. For example, cohesin stabilization disrupts positioning of telomeres and nearby DNA in the nuclear interior, which is a characteristic of genome reorganization in early interphase [33, 61]. While we do not find specific effects on gene expression in LADs, it remains unclear whether other heterochromatic phenotypes such as telomere maintenance and replication timing are affected by nuclear repositioning following perturbed cohesin dynamics [8, 62].
It was previously reported that CTCF is required for the peripheral positioning of integrated LAD fragments . This generally contradicts with the small effects that we observe upon CTCF depletion. We speculate that this strong effect is a consequence of the small size of the LAD fragment, which in our data correlates with decreasing NL interactions. Mixing of nearby chromatin — typically inhibited by CTCF [32, 63] — may drag small, isolated LADs into the nuclear interior.
A similar dependency for the peripheral position of LAD fragments was shown for EZH2, the methyltransferase responsible for H3K27me3 deposition . This differs from our results, which show that inhibition of EZH2 (with GSK126) does not affect NL interactions, alone or combined with CTCF depletion. Possibly, this discrepancy reflects cell type differences in NL affinity. H3K27me3 (and presumably also EZH2) is enriched inside LADs for some cell types, such as HCT116 cells, but not for mESCs (Fig. 1C, Additional file 1: S1F). In accordance with this reasoning, peripheral positioning of H3K27me3 is a transient phenotype during the cell cycle, development, and senescence [28, 29, 64].
Previous work illustrated that chromatin positioning at the NL is not required for genome compartmentalization . Vice-versa, our data suggests that genome compartmentalization, which is strongly and rapidly weakened upon WAPL and CTCF/WAPL depletion [26, 49], is also not required for NL positioning.
What then determines LAD border positioning?
Overall, our data indicate that CTCF and cohesin are not required for LAD border positioning in mESCs, but only contribute to sharpening of the borders. It thus remains to be elucidated what demarcates LADs. While we cannot rule out that an undiscovered factor is involved, it is also possible that there are no specific characteristics of LAD borders. Rather, the factors that mediate NL interactions may be tightly controlled and result in sharp LAD borders.
Constitutive LADs and their borders are conserved between human and mouse cells . While the nucleotide sequences are highly divergent, both species maintain a high AT-content in these LAD domains that presumably mediates interactions with specific NL components . This may involve H3K9me2, which is evolutionary conserved, is retained throughout mitosis, and quickly re-establishes peripheral positioning in daughter cells . It is, however, likely that additional mechanisms play a role as well. For example, similar to constitutive LADs, mESC-specific LADs have a high AT-content . This is in accordance with a model that LADs in stem cells reflect a “basal” state of chromosome organization, and suggests that AT-rich DNA somehow can be targeted to the NL. During differentiation, the basal organization could then be rearranged by a different NL composition that interacts with chromatin marks such as H3K27me3. In this model, CTCF, cohesin, and transcription are not integral to the general pattern of LAD domains. Instead, these factors can modulate NL interactions via forces, such as the formation of loops, that counteract peripheral positioning.
We systematically investigated how NL interactions are affected by proteins involved in the loop extrusion process. Remarkably, while cohesin perturbation drastically affects genome compartmentalization, NL interaction patterns are largely unaffected. Rather, CTCF and cohesin promote focal detachment from the NL and affect peripheral positioning of the genome quantitatively.
E14Tg2a mouse embryonic stem cells (mESCs) (129/Ola isogenic background) and derived clones were cultured in serum-free DMEM/F12 (Gibco) and Neurobasal (Gibco) medium (1:1), supplemented with N-2 (Gibco), B-27 (Gibco), BSA (0.05%; Gibco), 104 U of leukemia inhibitory factor (LIF; Millipore), MEK inhibitor PD0325901 (1 μM; Selleckchem), GSK-3β inhibitor CHIR99021 (3 μM; Cayman Chemical), and 1-thioglycerol (1.5 × 10−4 M; Sigma-Aldrich) on 0.1% gelatin-coated plates. Cells were passaged every 2 days. Cells were seeded and incubated overnight before starting protein depletion experiments, at the following densities: for a 96-h time course, 35,000 and 150,000 cells were seeded in 6-well and 10-cm plates, respectively; for a 24-h time course, 0.5 million and 1.5 million cells were seeded in 6-well and 10-cm plates, respectively. During these time courses, the medium was refreshed or cells were split 1:10 every 2 days.
Protein depletion was induced by treating cells with a final concentration of 500 μM auxin (IAA) (I5148-10G, Sigma-Aldrich). Time series experiments were performed by inducing protein degradation at different time points and collecting all samples at the end of the time course.
F121-9-CASTx129 mESC were cultured in 2i conditions according to the 4D Nucleome guidelines (https://data.4dnucleome.org/biosources/4DNSRMG5APUM/). These cells were differentiated into neural precursor cells (mNPCs) and cultured as described .
Cells were tested for mycoplasma every 3 months.
To verify protein depletion, AID-tagged mESCs were treated for 6 h with H2O or IAA and harvested. To obtain the nuclear soluble fraction, cells were resuspended in 500 μL of low salt buffer (final concentration: 10 mM HEPES, 50 mM NaCl, 1 mM EDTA, 1mM DTT, and 1X Protease Inhibitor Cocktail (11697498001, Sigma-Aldrich)) and incubated on ice for 15 min, followed by adding 500 μL of low salt/0.4% NP-40 buffer to reach a final concentration of NP-40 at 0.2%. These cells were then rotated at 4 °C for 15 min and centrifuged at 6000 g for 10 min at 4 °C. The obtained cell pellets were resuspended in high salt buffer (final concentration: 10 mM HEPES, 420 mM NaCl, 1 mM EDTA, 10% glycerol, and 1X Protease Inhibitor Cocktail) at a density of 5 million cells per 100 μl buffer and then rotated overnight at 4 °C. The supernatant was collected for subsequent experiments. For the whole cell lysates, the cells were lysed in RIPA lysis buffer (150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, and 25 mM Tris (pH 7.4)).
The nuclear extracts were used for western blot analysis of CTCF, WAPL, and ACTB, and the whole cell lysates were used for western blot analysis of RAD21 and HSP90. Precast gradient SDS-PAGE gels (NuPAGETM 4 to 12%, Bis-Tris 1.0 mm, Mini Protein Gel, 15-well, NP0323BOX, ThermoFisher SCIENTIFIC) were used to separate the proteins and transferred to preactivated PVDF membranes on a Trans-Blot Turbo Transfer System (Bio-Rad). The blots were incubated with primary antibodies against the following proteins overnight at 4 °C: (1) CTCF (1:1000; 07-729, Merck Millipore), (2) WAPL (1:1000; 16370-1-AP, Proteintech), (3) ACTB (1:5000; ab8227, Abcam), (4) RAD21 (1:1000; ab154769, Abcam), and (5) HSP90 (1:2000; 13171-1-AP). After incubation, the blots were washed three times with 0.1% Tween-20 in TBS (TBST) and then incubated with secondary antibody against rabbit IgG at room temperature for 1 h, again followed by three washes with TBST.
For H3K27me3 detection, mESCs were collected and lysed in lysis buffer (Tris pH 8.0 50 mM, EDTA 1mM, SDS 1 %) and sonicated. A total of 25 μg of total protein extracts was run on a gradient (15-4%) Polyacrylamide gel (MINIi-PROTEAN TGX Precast Gels, Biorad). Proteins were transferred on the nitro-cellulose membrane and checked with Red Ponceau staining for efficient transfer. The membranes were incubated with blocking buffer (5% milk powder in TBST) for 1 h at room temperature and afterwards incubated with primary antibody (anti-H3K27me3, Diagenode # C15410195, 1:1000) in blocking buffer overnight at 4 °C. Membranes were washed with TBST and secondary antibody incubation was performed for 2 h at room temperature in blocking buffer.
Protein detection was performed using Biorad Clarity Max ECL substrates and a ChemiDoc MP Imaging System (Bio-Rad).
Cell cycle analysis
Cell cycle analysis was performed with the Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Invitrogen). Briefly, for 1.5 h, mESCs were incubated with 10 μM Click-iT EdU and fixed and permeabilized. Cells were incubated with the Click-iT Plus reaction cocktail for 30 min at room temperature (protected from light) to detect EdU and stained with 4′,6-diamidino-2-phenylindole (DAPI). DAPI and EdU signals were quantified on a BD LSRFortessa analyzer and processed with FlowJo 10.3.
LaminB1 DamID and pA-DamID
LaminB1 DamID was performed as described previously [56, 67]. Briefly, F121-9-CASTx129 mESCs and mNPCs were lentivirally transduced in a 6-well plate with Dam control or (mouse) Dam-LaminB1 constructs. Dam was under the control of the human PGK1 promoter and fused to a destabilization domain. Genomic DNA was isolated 3 days after transduction (ISOLATE II Genomic DNA kit, Bioline BIO-52067), without stabilization of the Dam fusions to keep expression at low levels. m6A-marked DNA was enriched using a sequence of DpnI digestion, PCR adapter ligation, DpnII digestion, and PCR amplification. The resulting amplified material was processed for high-throughput sequencing using an in-house library preparation procedure and sequenced for single-end 140-bp reads on an Illumina HiSeq 2500. Approximately 10 million reads were sequenced for every condition.
pA-DamID LaminB1 maps were generated as described . One million E14Tg2a mESCs were collected by centrifugation (500 g, 3 min) and washed sequentially in ice-cold PBS and digitonin wash buffer (DigWash) (20 mM HEPES-KOH pH 7.5, 150 mM NaCl, 0.5 mM spermidine, 0.02% digitonin, cOmplete Protease Inhibitor Cocktail). Cells were rotated for 2 h at 4 °C in 200 μL DigWash with 1:400 Lamin B1 antibody (Abcam, ab16048, rabbit), followed by a wash step with DigWash. This was repeated with a 1:200 pA-Dam solution (~60 NEB Dam units), followed by 2 wash steps. Dam activity was induced by an incubation for 30 min at 37 °C in 100 μL DigWash supplemented with 80 μM SAM while gently shaking (500 rpm). Genomic DNA was isolated and DNA was processed similar to DamID, except that the DpnII digestion was omitted and 65-bp reads were sequenced. For every condition, another 1 million cells were processed in only DigWash and during Dam activation incubated with 4 units of Dam enzyme (NEB, M0222L). This Dam control sample serves to account for DNA accessibility and amplification biases.
ATAC-seq libraries were prepared as previously described . Cells were permeabilized and tagmented with in-house-generated Tn5 transposase, after which DNA fragments were amplified with two sequential nine-cycle PCR runs. Fragments smaller than 700 bp were selected with SPRI beads. ATAC-seq libraries were sequenced with paired-end mode using a 75-cycle kit on an Illumina NextSeq 550.
ChIP-seq was performed as described but with small modifications . mESCs and mNPCs were mixed with 10% HEK293T cells (as internal reference) and crosslinked for 10 min with 1% FA. The reaction was quenched with 2.0 M glycine. After cell lysis, a Bioruptor Plus sonication device (Diagenode) was used to fragment chromatin to approximately 300-bp fragments. Antibodies against CTCF (07-729, Merck Millipore; 5 μL per ChIP) or H3K27me3 (PAB-195-050, Diagenode; 5 μL per ChIP) were coupled with Protein G beads (Thermo Fisher Scientific) and incubated with fragmented chromatin overnight at 4 °C. After washing, chromatin was eluted from the beads and crosslinking was reversed. Released DNA fragments were purified with the MinElute PCR Purification kit (Qiagen). The purified DNA fragments were prepared with the KAPA HTP Library Preparation kit (Roche) and single-end sequenced with a 65-cycle kit on an Illumina HiSeq 2500 (CTCF) or a 75-cycle kit on a NextSeq 550 (H3K27me3).
RNA was isolated using a standard TRIzol RNA isolation protocol (Ambion). Cells were lysed with 1 mL of TRIzol reagent, after which 200 μL chloroform was added and the mixture was vortexed. Following centrifugation at 12,000 g at for 15 min at 4 °C, the upper phase was homogenized with 0.5 mL of 100% isopropanol. Samples were incubated at room temperature for 10 min and centrifuged at 4 °C for another 10 min. The resulting RNA pellet was washed with ice-cold 75% ethanol, dried at room temperature, and resuspended in RNase-free water. The isolated RNA was treated with DNase using the RNeasy Mini kit (Qiagen). RNA-seq libraries were prepared using a TruSeq Stranded RNA LT Kit (Illumina). The libraries were sequenced for single-end 65-bp reads on an Illumina HiSeq 2500.
DamID and pA-DamID
DamID and pA-DamID data was processed as described . Briefly, the adapter sequence was trimmed with cutadapt 1.11 before mapping the remaining genomic DNA sequence to mm10 with bwa mem 0.7.17. The following steps were performed with custom R scripts. Reads with a mapping quality of at least 10 and overlapping the ends of a GATC fragment were counted in 10-kb genomic bins. Counts were normalized to 1 million reads per sample and a log2-ratio over the Dam control sample was calculated with a pseudo count of 1. At least 2 biological replicates were generated for every experimental condition and the average score was used for downstream analyses. Log2 ratios were converted to z-scores to correct for differences in dynamic range between experiments (Additional file 1: Fig. S2H-I).
LADs were determined using hidden Markov modeling on the average NL interaction profile between biological replicates (https://github.com/gui11aume/HMMt). For Fig. 1A–C, the LAD definition based on wildtype mESC (F121-9-CASTx129 strain) LaminB1 DamID was used. For the remaining analyses, the LAD definition was based on PT mESC (E14Tg2a strain) LaminB1 pA-DamID, except when otherwise stated. In order to capture newly formed LADs, a consensus LAD model between all experimental conditions was used for Figs. 4, 5, and 6 (a union set of LADs called in PT and AID-tagged cell lines up to 24 h of IAA addition).
LAD border classification
LAD borders were classified as CTCF borders if a CTCF binding site was within 20 kb outside the LAD (overlapping with the enriched CTCF density). Borders within 10 kb of an active gene (FPKM > 1) were flagged and not used in downstream analyses unless otherwise indicated. LAD borders were assigned to a loop anchor if this was positioned within 20 kb. To determine CTCF orientation at LAD borders, the CTCF motif (JASPAR MA0139.1 ) was used to infer the orientation of CTCF binding sites with FIMO (MEME suite) . LAD borders with a single CTCF orientation were assigned this orientation, while LAD borders near multiple CTCF orientations or unassigned CTCF binding sites were assigned “ambiguous.” Deeptools 3.5.0  and custom R scripts were used to calculate and visualize scores relative to LAD borders and LAD features (i.e. active genes, CTCF binding sites).
Nuclear lamina detachment score
The detachment score was defined as the difference in mean NL interaction scores between the flanking region (distances between 50 and 100 kb, both sides) and the CTCF binding site (up to 10 kb). To prevent confounding factors for transcription and border positioning, CTCF binding sites were filtered to be at least 100 kb from active genes and LAD borders.
RNA-seq and TT-seq
RNA-seq reads were mapped against the mm10 reference genome with TopHat2 2.1.1  and filtered for a mapping quality of at least 10. Read counts for Ensembl genes (GRCm38.92; exons only) were determined with HTSeq 0.9.1. DESeq2 1.30.1  was used to call differentially expressed genes by testing for a log2-fold difference of 0.5 with a false discovery rate of 0.05. Principal component analysis was performed with “plotPCA()” from DESeq2, using the top 5000 most variable genes. FPKM values were calculated with “fpkm()” from DESeq2, using the combined exon length as gene length. Similar downstream processing was applied to call differential expression in the TT-seq data .
ATAC-seq reads were mapped to mm10 with bwa mem 0.7.15-r1140. SAMtools 1.9  was used to filter reads for a mapping quality of at least 15 and discard optimal PCR duplicates. Genomic coverage was determined with deeptools 3.0 .
ChIP-seq data were analyzed as previously reported . Reads were mapped to a concatenated reference genome (mm10 and hg19) using bowtie2 220.127.116.11 . Reads were filtered for a mapping quality of at least 15 and optical PCR duplicates were removed with SAMtools 1.9. The normalization factor to scale the spike-in HEK293T reads (mapping to hg19) to 1 million reads was used as the scaling factor in deeptools 3.0 to determine genomic coverage. Peak calling was performed with MACS2 18.104.22.16860309  at a q-value cutoff of 0.01.
The external datasets that have been used in this study have been referred to in the main text and figure legends. Data identifiers are listed in Additional file 2: Table S1.
Availability of data and materials
The genomic datasets used in this article [77,78,79,80,81,82,83,84,85,86,87,88,89,90,91,92,93,94,95,96,97,98,99,100,101,102,103,104,105,106,107,108,109,110,111,112,113,114,115,116,117,118,119,120,121,122,123,124,125,126,127] are publicly available, as specified in Additional file 2: Table S1. The computational code used to analyze the data are available at https://doi.org/10.5281/zenodo.6977460 . Laboratory notes of most laboratory experiments are available at https://osf.io/jw74g/ .
Guelen L, Pagie L, Brasset E, Meuleman W, Faza MB, Talhout W, et al. Domain organization of human chromosomes revealed by mapping of nuclear lamina interactions. Nature. 2008;453:948–51.
van Steensel B, Belmont AS. Lamina-associated domains: links with chromosome architecture, heterochromatin, and gene repression. Cell. 2017;169:780–91.
Lochs SJA, Kefalopoulou S, Kind J. Lamina associated domains and gene regulation in development and cancer. Cells. 2019;8:271.
Briand N, Collas P. Lamina-associated domains: peripheral matters and internal affairs. Genome Biol. 2020;21:85.
Wen B, Wu H, Shinkai Y, Irizarry RA, Feinberg AP. Large histone H3 lysine 9 dimethylated chromatin blocks distinguish differentiated from embryonic stem cells. Nat Genet. 2009;41:246–50.
Peric-Hupkes D, Meuleman W, Pagie L, Bruggeman SW, Solovei I, Brugman W, et al. Molecular maps of the reorganization of genome-nuclear lamina interactions during differentiation. Mol Cell. 2010;38:603–13.
Kind J, Pagie L, Ortabozkoyun H, Boyle S, de Vries SS, Janssen H, et al. Single-cell dynamics of genome-nuclear lamina interactions. Cell. 2013;153:178–92.
Pope BD, Ryba T, Dileep V, Yue F, Wu W, Denas O, et al. Topologically associating domains are stable units of replication-timing regulation. Nature. 2014;515:402–5.
Poleshko A, Smith CL, Nguyen SC, Sivaramakrishnan P, Wong KG, Murray JI, et al. H3K9me2 orchestrates inheritance of spatial positioning of peripheral heterochromatin through mitosis. Elife. 2019;8:e49278.
Bian Q, Khanna N, Alvikas J, Belmont AS. beta-Globin cis-elements determine differential nuclear targeting through epigenetic modifications. J Cell Biol. 2013;203:767–83.
Therizols P, Illingworth RS, Courilleau C, Boyle S, Wood AJ, Bickmore WA. Chromatin decondensation is sufficient to alter nuclear organization in embryonic stem cells. Science. 2014;346:1238–42.
Harr JC, Luperchio TR, Wong X, Cohen E, Wheelan SJ, Reddy KL. Directed targeting of chromatin to the nuclear lamina is mediated by chromatin state and A-type lamins. J Cell Biol. 2015;208:33–52.
Gonzalez-Sandoval A, Towbin BD, Kalck V, Cabianca DS, Gaidatzis D, Hauer MH, et al. Perinuclear anchoring of H3K9-methylated chromatin stabilizes induced cell fate in C. elegans embryos. Cell. 2015;163:1333–47.
Brueckner L, Zhao PA, van Schaik T, Leemans C, Sima J, Peric-Hupkes D, et al. Local rewiring of genome-nuclear lamina interactions by transcription. EMBO J. 2020;39:e103159.
Solovei I, Wang AS, Thanisch K, Schmidt CS, Krebs S, Zwerger M, et al. LBR and lamin A/C sequentially tether peripheral heterochromatin and inversely regulate differentiation. Cell. 2013;152:584–98.
Chen S, Luperchio TR, Wong X, Doan EB, Byrd AT, Roy Choudhury K, et al. A lamina-associated domain border governs nuclear lamina interactions, transcription, and recombination of the Tcrb locus. Cell Rep. 2018;25:1729–40 e1726.
Wendt KS, Yoshida K, Itoh T, Bando M, Koch B, Schirghuber E, et al. Cohesin mediates transcriptional insulation by CCCTC-binding factor. Nature. 2008;451:796–801.
Hansen AS, Pustova I, Cattoglio C, Tjian R, Darzacq X. CTCF and cohesin regulate chromatin loop stability with distinct dynamics. Elife. 2017;6:e25776.
Fudenberg G, Imakaev M, Lu C, Goloborodko A, Abdennur N, Mirny LA. Formation of chromosomal domains by loop extrusion. Cell Rep. 2016;15:2038–49.
Davidson IF, Bauer B, Goetz D, Tang W, Wutz G, Peters JM. DNA loop extrusion by human cohesin. Science. 2019;366:1338–45.
Kim Y, Yu H. Shaping of the 3D genome by the ATPase machine cohesin. Exp Mol Med. 2020;52:1891–7.
Li Y, Haarhuis JHI, Sedeno Cacciatore A, Oldenkamp R, van Ruiten MS, Willems L, et al. The structural basis for cohesin-CTCF-anchored loops. Nature. 2020;578:472–6.
Nora EP, Caccianini L, Fudenberg G, So K, Kameswaran V, Nagle A, et al. Molecular basis of CTCF binding polarity in genome folding. Nat Commun. 2020;11:5612.
Schwarzer W, Abdennur N, Goloborodko A, Pekowska A, Fudenberg G, Loe-Mie Y, et al. Two independent modes of chromatin organization revealed by cohesin removal. Nature. 2017;551:51–6.
Haarhuis JHI, van der Weide RH, Blomen VA, Yanez-Cuna JO, Amendola M, van Ruiten MS, et al. The Cohesin release factor WAPL restricts chromatin loop extension. Cell. 2017;169:693–707 e614.
Liu NQ, Maresca M, van den Brand T, Braccioli L, Schijns M, Teunissen H, et al. WAPL maintains a cohesin loading cycle to preserve cell-type-specific distal gene regulation. Nat Genet. 2021;53:100–9.
Kind J, Pagie L, de Vries SS, Nahidiazar L, Dey SS, Bienko M, et al. Genome-wide maps of nuclear lamina interactions in single human cells. Cell. 2015;163:134–47.
Tran JR, Paulson DI, Moresco JJ, Adam SA, Yates JR, Goldman RD, et al. An APEX2 proximity ligation method for mapping interactions with the nuclear lamina. J Cell Biol. 2021;220:e202002129.
Sadaie M, Salama R, Carroll T, Tomimatsu K, Chandra T, Young AR, et al. Redistribution of the Lamin B1 genomic binding profile affects rearrangement of heterochromatic domains and SAHF formation during senescence. Genes Dev. 2013;27:1800–8.
Le HQ, Ghatak S, Yeung CY, Tellkamp F, Gunschmann C, Dieterich C, et al. Mechanical regulation of transcription controls Polycomb-mediated gene silencing during lineage commitment. Nat Cell Biol. 2016;18:864–75.
Nishimura K, Fukagawa T, Takisawa H, Kakimoto T, Kanemaki M. An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nat Methods. 2009;6:917–22.
Nora EP, Goloborodko A, Valton AL, Gibcus JH, Uebersohn A, Abdennur N, et al. Targeted degradation of CTCF decouples local insulation of chromosome domains from genomic compartmentalization. Cell. 2017;169:930–44 e922.
van Schaik T, Vos M, Peric-Hupkes D, Hn Celie P, van Steensel B. Cell cycle dynamics of lamina-associated DNA. EMBO Rep. 2020;21:e50636.
Schmidl C, Rendeiro AF, Sheffield NC, Bock C. ChIPmentation: fast, robust, low-input ChIP-seq for histones and transcription factors. Nat Methods. 2015;12:963–5.
ENCODE Consortium Project. An integrated encyclopedia of DNA elements in the human genome. Nature. 2012;489:57–74.
Bonev B, Mendelson Cohen N, Szabo Q, Fritsch L, Papadopoulos GL, Lubling Y, et al. Multiscale 3D genome rewiring during mouse neural development. Cell. 2017;171:557–72 e524.
Kelso TWR, Porter DK, Amaral ML, Shokhirev MN, Benner C, Hargreaves DC. Chromatin accessibility underlies synthetic lethality of SWI/SNF subunits in ARID1A-mutant cancers. Elife. 2017;6:e30506.
Dai Z, Mentch SJ, Gao X, Nichenametla SN, Locasale JW. Methionine metabolism influences genomic architecture and gene expression through H3K4me3 peak width. Nat Commun. 2018;9:1955.
Essletzbichler P, Konopka T, Santoro F, Chen D, Gapp BV, Kralovics R, et al. Megabase-scale deletion using CRISPR/Cas9 to generate a fully haploid human cell line. Genome Res. 2014;24:2059–65.
Hojfeldt JW, Laugesen A, Willumsen BM, Damhofer H, Hedehus L, Tvardovskiy A, et al. Accurate H3K27 methylation can be established de novo by SUZ12-directed PRC2. Nat Struct Mol Biol. 2018;25:225–32.
Tastemel M, Gogate AA, Malladi VS, Nguyen K, Mitchell C, Banaszynski LA, et al. Transcription pausing regulates mouse embryonic stem cell differentiation. Stem Cell Res. 2017;25:250–5.
Joshi O, Wang SY, Kuznetsova T, Atlasi Y, Peng T, Fabre PJ, et al. Dynamic reorganization of extremely long-range promoter-promoter interactions between two states of pluripotency. Cell Stem Cell. 2015;17:748–57.
Marks H, Kalkan T, Menafra R, Denissov S, Jones K, Hofemeister H, et al. The transcriptional and epigenomic foundations of ground state pluripotency. Cell. 2012;149:590–604.
von Meyenn F, Iurlaro M, Habibi E, Liu NQ, Salehzadeh-Yazdi A, Santos F, et al. Impairment of DNA methylation maintenance is the main cause of global demethylation in naive embryonic stem cells. Mol Cell. 2016;62:848–61.
de Wit E, Vos ES, Holwerda SJ, Valdes-Quezada C, Verstegen MJ, Teunissen H, et al. CTCF binding polarity determines chromatin looping. Mol Cell. 2015;60:676–84.
Guo Y, Xu Q, Canzio D, Shou J, Li J, Gorkin DU, et al. CRISPR inversion of CTCF sites alters genome topology and enhancer/promoter eunction. Cell. 2015;162:900–10.
Sanborn AL, Rao SS, Huang SC, Durand NC, Huntley MH, Jewett AI, et al. Chromatin extrusion explains key features of loop and domain formation in wild-type and engineered genomes. Proc Natl Acad Sci U S A. 2015;112:E6456–65.
Rowley MJ, Corces VG. Organizational principles of 3D genome architecture. Nat Rev Genet. 2018;19:789–800.
Liu NQ, Magnitov M, Schijns M, van Schaik T, van der Weide RH, Teunissen H, et al. Rapid depletion of CTCF and cohesin proteins reveals dynamic features of chromosome architecture. bioRxiv. 2021. https://doi.org/10.1101/2021.08.27.457977.
Rhodes JDP, Feldmann A, Hernandez-Rodriguez B, Diaz N, Brown JM, Fursova NA, et al. Cohesin disrupts polycomb-dependent chromosome interactions in embryonic stem cells. Cell Rep. 2020;30:820–35 e810.
Wen B, Wu H, Loh YH, Briem E, Daley GQ, Feinberg AP. Euchromatin islands in large heterochromatin domains are enriched for CTCF binding and differentially DNA-methylated regions. BMC Genomics. 2012;13:566.
Tumbar T, Belmont AS. Interphase movements of a DNA chromosome region modulated by VP16 transcriptional activator. Nat Cell Biol. 2001;3:134–9.
Isoda T, Moore AJ, He Z, Chandra V, Aida M, Denholtz M, et al. Non-coding transcription instructs chromatin folding and compartmentalization to dictate enhancer-promoter communication and T cell fate. Cell. 2017;171:103–19 e118.
Buenrostro JD, Giresi PG, Zaba LC, Chang HY, Greenleaf WJ. Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position. Nat Methods. 2013;10:1213–8.
Riising EM, Comet I, Leblanc B, Wu X, Johansen JV, Helin K. Gene silencing triggers polycomb repressive complex 2 recruitment to CpG islands genome wide. Mol Cell. 2014;55:347–60.
Leemans C, van der Zwalm MCH, Brueckner L, Comoglio F, van Schaik T, Pagie L, et al. Promoter-intrinsic and local chromatin features determine gene repression in LADs. Cell. 2019;177:852–64 e814.
Rao SSP, Huang SC, Glenn St Hilaire B, Engreitz JM, Perez EM, Kieffer-Kwon KR, et al. Cohesin loss eliminates all loop domains. Cell. 2017;171:305–20 e324.
Wutz G, Varnai C, Nagasaka K, Cisneros DA, Stocsits RR, Tang W, et al. Topologically associating domains and chromatin loops depend on cohesin and are regulated by CTCF, WAPL, and PDS5 proteins. EMBO J. 2017;36:3573–99.
Jose CC, Xu B, Jagannathan L, Trac C, Mallela RK, Hattori T, et al. Epigenetic dysregulation by nickel through repressive chromatin domain disruption. Proc Natl Acad Sci U S A. 2014;111:14631–6.
Chuang CH, Carpenter AE, Fuchsova B, Johnson T, de Lanerolle P, Belmont AS. Long-range directional movement of an interphase chromosome site. Curr Biol. 2006;16:825–31.
Crabbe L, Cesare AJ, Kasuboski JM, Fitzpatrick JA, Karlseder J. Human telomeres are tethered to the nuclear envelope during postmitotic nuclear assembly. Cell Rep. 2012;2:1521–9.
Burla R, La Torre M, Saggio I. Mammalian telomeres and their partnership with lamins. Nucleus. 2016;7:187–202.
Luppino JM, Park DS, Nguyen SC, Lan Y, Xu Z, Yunker R, et al. Cohesin promotes stochastic domain intermingling to ensure proper regulation of boundary-proximal genes. Nat Genet. 2020;52:840–8.
Prokopuk L, Stringer JM, Hogg K, Elgass KD, Western PS. PRC2 is required for extensive reorganization of H3K27me3 during epigenetic reprogramming in mouse fetal germ cells. Epigenetics Chromatin. 2017;10:7.
Falk M, Feodorova Y, Naumova N, Imakaev M, Lajoie BR, Leonhardt H, et al. Heterochromatin drives compartmentalization of inverted and conventional nuclei. Nature. 2019;570:395–9.
Meuleman W, Peric-Hupkes D, Kind J, Beaudry JB, Pagie L, Kellis M, et al. Constitutive nuclear lamina-genome interactions are highly conserved and associated with A/T-rich sequence. Genome Res. 2013;23:270–80.
Vogel MJ, Peric-Hupkes D, van Steensel B. Detection of in vivo protein-DNA interactions using DamID in mammalian cells. Nat Protoc. 2007;2:1467–78.
Liu NQ, Ter Huurne M, Nguyen LN, Peng T, Wang SY, Studd JB, et al. The non-coding variant rs1800734 enhances DCLK3 expression through long-range interaction and promotes colorectal cancer progression. Nat Commun. 2017;8:14418.
Fornes O, Castro-Mondragon JA, Khan A, van der Lee R, Zhang X, Richmond PA, et al. JASPAR 2020: update of the open-access database of transcription factor binding profiles. Nucleic Acids Res. 2020;48:D87–92.
Grant CE, Bailey TL, Noble WS. FIMO: scanning for occurrences of a given motif. Bioinformatics. 2011;27:1017–8.
Ramirez F, Dundar F, Diehl S, Gruning BA, Manke T. deepTools: a flexible platform for exploring deep-sequencing data. Nucleic Acids Res. 2014;42:W187–91.
Kim D, Pertea G, Trapnell C, Pimentel H, Kelley R, Salzberg SL. TopHat2: accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions. Genome Biol. 2013;14:R36.
Love MI, Huber W, Anders S. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol. 2014;15:550.
Danecek P, Bonfield JK, Liddle J, Marshall J, Ohan V, Pollard MO, et al. Twelve years of SAMtools and BCFtools. Gigascience. 2021;10:giab008.
Langmead B, Salzberg SL. Fast gapped-read alignment with Bowtie 2. Nat Methods. 2012;9:357–9.
Zhang Y, Liu T, Meyer CA, Eeckhoute J, Johnson DS, Bernstein BE, et al. Model-based analysis of ChIP-Seq (MACS). Genome Biol. 2008;9:R137.
van Steensel B. LaminB1 DamID of f1219castx129T2 Tier 2 cells. Dataset 4DNESYPMROEJ. 4DNucleome. 2018. https://data.4dnucleome.org/experiment-set-replicates/4DNESYPMROEJ.
van Schaik T, Liu NQ, de Wit E, van Steensel B. Rapid depletion of the cohesin subunits and CTCF reveals their role in maintaining high-order genome architecture [DamID and ChIP]. Dataset GSE183958. Gene Expression Omnibus. 2022. https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE183958.
van Steensel B. LaminB1 DamID of H1-hESC Tier 1 cells. Dataset 4DNESXKBPZKQ. 4DNucleome. 2017. https://data.4dnucleome.org/experiment-set-replicates/4DNESXKBPZKQ.
van Steensel B. LaminB1 DamID of Hap1 Tier 2 cells. Dataset 4DNESUK5H9Y8. 4DNucleome. 2018. https://data.4dnucleome.org/experiment-set-replicates/4DNESUK5H9Y8.
van Steensel B. LaminB1 DamID of K562 Tier 2 cells. Dataset 4DNESTAJJM3X. 4DNucleome. 2018. https://data.4dnucleome.org/experiment-set-replicates/4DNESTAJJM3X.
van Steensel B. LaminB1 DamID of HCT116 Tier 2 cells. Dataset 4DNES24XA7U8. 4DNucleome. 2018. https://data.4dnucleome.org/experiment-set-replicates/4DNES24XA7U8.
Liu N, de Wit E. Dynamic cohesin is required for lineage specific distal gene regulation. Dataset GSM3992899. Gene Expression Omnibus. 2021. https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM3992899.
Myers R. CTCF ChIP-seq protocol v041610.2 on human H1-hESC. Dataset ENCSR000BNH. ENCODE. 2011. https://www.encodeproject.org/experiments/ENCSR000BNH.
van der Weide RH, de Wit E. The cohesin release factor WAPL restricts chromatin loop extension. Dataset GSM2493878. Gene Expression Omnibus. 2017. https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM2493878.
Schmidl C, Rendeiro AF, Sheffield NC, Bock C. ChIPmentation: fast, cheap, low-input ChIP-seq for histones and transcription factors. Dataset SRR2085872. Sequence Read Archive. 2015. https://www.ncbi.nlm.nih.gov/sra/?term=SRR2085872.
Bernstein B. CTCF in HCT116 cell line. Dataset ENCSR240PRQ. ENCODE. 2017. https://www.encodeproject.org/experiments/ENCSR240PRQ.
van Schaik T, Liu NQ, van Steensel B. Rapid depletion of the cohesin subunits and CTCF reveals their role in maintaining high-order genome architecture [DamID and ChIP]. Dataset GSE181693. Gene Expression Omnibus. 2022. https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE181693.
van Steensel B. pADamID for LaminB1 on Hap1 wildtype cells. Dataset 4DNESFWILAC9. 4DNucleome. 2019. https://data.4dnucleome.org/experiment-set-replicates/4DNESFWILAC9.
van Steensel B. pADamID for LaminB1 on K562 wildtype cells. Dataset 4DNESUMP6SS1. 4DNucleome. 2019. https://data.4dnucleome.org/experiment-set-replicates/4DNESUMP6SS1.
van Steensel B. pADamID for LaminB1 on HCT116 wildtype cells. Dataset 4DNESWB729QB. 4DNucleome. 2019. https://data.4dnucleome.org/experiment-set-replicates/4DNESWB729QB.
Liu N, de Wit E. Dynamic cohesin is required for lineage specific distal gene regulation. Dataset GSE135180. Gene Expression Omnibus. 2021. https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE135180.
Liu N, de Wit E. Rapid depletion of the cohesin subunits and CTCF reveals their role in maintaining high-order genome architecture. Dataset GSE181849. Gene Expression Omnibus. 2022. https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE181849.
Liu N, de Wit E. Rapid depletion of the cohesin subunits and CTCF reveals their role in maintaining high-order genome architecture. Dataset GSE181846. Gene Expression Omnibus. 2022. https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE181846.
Bonev B, Mendelson Cohen N, Szabo Q, Fritsch L, Papadopoulos G, Lubling Y, Xu X, Lv X, Hugnot J, Tanay A, Cavalli G. Multi-scale 3D genome rewiring during mouse neural development. Dataset GSE96107. Gene Expression Omnibus. 2017. https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE96107.
Liu N, de Wit E. Dynamic cohesin is required for lineage specific distal gene regulation. Dataset GSM3992901. Gene Expression Omnibus. 2021. https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM3992901.
Tastemel M, Gogate AA, Malladi V, Mitchell C, Banaszynski LA, Bai X. Transcription pausing regulates mouse embryonic stem cell differentiation [ATAC-seq]. Dataset GSM2651155. Gene Expression Omnibus. 2017. https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM2651155.
Joshi O, Wang SY, Atlasi Y, Peng T, Saeed S, Handoko L, Kuznetsova T. Dynamic reorganization of extremely long-range promoter-promoter interactions between two states of pluripotency. Dataset GSM1856424. Gene Expression Omnibus. 2015. https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1856424.
Marks H, Menafra R, Kalkan T, Denissov S, Jones K, Hofemeister H, Nichols J, Kranz A, Stewart AF, Smith A, Stunnenberg HG. Epigenome and transcriptome of naive pluripotent mouse embryonic stem (ES) cells cultured in 2i serum-free medium. Dataset GSM590112. Gene Expression Omnibus. 2012. https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM590112.
von Meyenn F, Iurlaro M, Petrini E, Reik W. Molecular mechanisms of global DNA demethylation to naïve pluripotency in ESC. Dataset GSM2051618. Gene Expression Omnibus. 2016. https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM2051618.
Joshi O, Wang SY, Atlasi Y, Peng T, Saeed S, Handoko L, Kuznetsova T. Dynamic reorganization of extremely long-range promoter-promoter interactions between two states of pluripotency. Dataset GSM1856426. Gene Expression Omnibus. 2015. https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM1856426.
Hojfeldt JW, Laugesen A, Willumsen BM, Damhofer H, Hedehus L, Helin K. Accurate H3K27 methylation patterns can be established de novo by SUZ12-directed PRC2. Dataset GSM2779214. Gene Expression Omnibus. 2018. https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM2779214.
Marks H, Menafra R, Kalkan T, Denissov S, Jones K, Hofemeister H, Nichols J, Kranz A, Stewart AF, Smith A, Stunnenberg HG. Epigenome and transcriptome of naive pluripotent mouse embryonic stem (ES) cells cultured in 2i serum-free medium. Dataset GSM590120. Gene Expression Omnibus. 2012. https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM590120.
Myers R. RAD21 ChIP-seq protocol v041610.2 on human H1-hESC. Dataset ENCSR000BLD. ENCODE. https://www.encodeproject.org/experiments/ENCSR000BLD.
Stamatoyannopoulos J. Dnase-seq of H1 cells. Dataset ENCSR794OFW. ENCODE. 2013. https://www.encodeproject.org/experiments/ENCSR794OFW.
Bernstein B. ChIP-seq of H3K4me1 in H1 cells. Dataset ENCSR631RJR. ENCODE. 2013. https://www.encodeproject.org/experiments/ENCSR631RJR.
Bernstein B. ChIP-seq of H3K4me3 in H1 cells. Dataset ENCSR019SQX. ENCODE. 2013. https://www.encodeproject.org/experiments/ENCSR019SQX.
Ren B. ChIP-seq of H3K9me3 in H1 cells. Dataset ENCSR883AQJ. ENCODE. 2013. https://www.encodeproject.org/experiments/ENCSR883AQJ.
Bernstein B. ChIP-seq of H3K27ac in H1 cells. Dataset ENCSR000ANP. ENCODE. 2011. https://www.encodeproject.org/experiments/ENCSR000ANP.
Bernstein B. ChIP-seq of H3K27me3 in H1 cells. Dataset ENCSR216OGD. ENCODE. 2013. https://www.encodeproject.org/experiments/ENCSR216OGD.
Ren B. ChIP-seq of H3K36me3 in H1 cells. Dataset ENCSR476KTK. ENCODE. 2013. https://www.encodeproject.org/experiments/ENCSR476KTK.
Myers R. RAD21 ChIP-seq protocol v042211.1 on human HCT-116. Dataset ENCSR000BSB. ENCODE. 2012. https://www.encodeproject.org/experiments/ENCSR000BSB.
Stamatoyannopoulos J. DNase-seq on human HCT-116. Dataset ENCSR000ENM. ENCODE. 2010. https://www.encodeproject.org/experiments/ENCSR000ENM.
Bernstein B. ChIP-seq of H3K4me1 in HCT116 cells. Dataset ENCSR161MXP. ENCODE. 2017. https://www.encodeproject.org/experiments/ENCSR161MXP.
Bernstein B. ChIP-seq of H3K4me3 in HCT116 cells. Dataset ENCSR333OPW. ENCODE. 2016. https://www.encodeproject.org/experiments/ENCSR333OPW.
Bernstein B. ChIP-seq of H3K9me3 in HCT116 cells. Dataset ENCSR179BUC. ENCODE. 2017. https://www.encodeproject.org/experiments/ENCSR179BUC.
Farnham P. ChIP-seq of H3K27ac in HCT116 cells. Dataset ENCSR000EUT. ENCODE. 2012. https://www.encodeproject.org/experiments/ENCSR000EUT.
Bernstein B. ChIP-seq of H3K27me3 in HCT116 cells. Dataset ENCSR810BDB. ENCODE. 2016. https://www.encodeproject.org/experiments/ENCSR810BDB.
Bernstein B. ChIP-seq of H3K36me3 in HCT116 cells. Dataset ENCSR091QXP. ENCODE. 2016. https://www.encodeproject.org/experiments/ENCSR091QXP.
Gingeras T. polyA plus RNA-seq of H1 cells. Dataset ENCFF000FET, ENCFF000FEU, ENCFF000DJM, ENCFF000DJN, ENCFF565ZQD, ENCFF953ZDW, ENCFF589VNC, ENCFF608OLY, ENCFF247WDK, ENCFF350HDB, ENCFF199CUO, ENCFF687WLZ, ENCFF199CUO, ENCFF567PCA. ENCODE. 2011. https://www.encodeproject.org/experiments/ENCSR000COU.
Gingeras T. polyA plus RNA-seq of K562 cells. Dataset ENCFF001RED, ENCFF001REG, ENCFF001RWD, ENCFF001RVV, ENCFF001RWE, ENCFF001RWF, ENCFF001RDD, ENCFF001RDE, ENCFF000HFF, ENCFF000HFH. ENCODE. 2014. https://www.encodeproject.org/experiments/ENCSR000AEM.
Wold B. RNA-seq on human HCT-116. Dataset ENCFF000DKT, ENCFF000DKW, ENCFF000DKV, ENCFF000DKX, ENCFF000DKY, ENCFF000DKU. ENCODE. 2012. https://www.encodeproject.org/experiments/ENCSR000CWM.
Kelso TW, Porter D, Hargreaves DC. ARID1A and ARID1B loss in HCT116 cells. Dataset GSM2719768, GSM2719769. Gene Expression Omnibus. 2017. https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE101966.
Dai Z, Locasale JW. Genome-wide H3K4me3 and gene expression before and after methionine restriction in human cancer cells and mouse liver. Dataset GSM2775145, GSM2775146. Gene Expression Omnibus. 2018. https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE103602.
van der Weide RH, de Wit E. The cohesin release factor WAPL restricts chromatin loop extension. Dataset GSM2493886, GSM2493887, GSM2493888, GSM2493898, GSM2493899, GSM2493900. Gene Expression Omnibus. 2017. https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE94993.
Essletzbichler P. RNA-seq of KBM7, HAP1, and eHAP cells. Dataset SRX655511, SRX655512. Sequence Read Archive. 2014. https://www.ncbi.nlm.nih.gov/sra/?term=SRX655511.
Liu N, de Wit E. Rapid depletion of the cohesin subunits and CTCF reveals their role in maintaining high-order genome architecture [ChIP-seq]. Dataset GSE181847. Gene Expression Omnibus. 2022. https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE181847.
van Schaik T. LADs CTCF cohesin analysis: Github; 2022. https://doi.org/10.5281/zenodo.6977460.
van Schaik T. CTCF and cohesin promote focal detachment of DNA from the nuclear lamina: lab notes, data and code. Open Sci Framework. 2022; https://osf.io/jw74g/.
We thank the NKI Digital Microscopy, Flow Cytometry, Genomics, Protein Production, and RHPC core facilities for technical assistance and members of our laboratories for helpful comments.
Peer review information
Andrew Cosgrove was the primary editor of this article and managed its editorial process and peer review in collaboration with the rest of the editorial team.
The review history is available as Additional file 3.
Supported by the NIH Common Fund “4D Nucleome” Program grant U54DK107965 (BvS), European Research Council Advanced Grant 694466 (“GoCADiSC”) (BvS), Marie Curie Fellowship 838555 (SGM), ERC Starting Grant 637587 (“HAP-PHEN”) (EdW), ERC Consolidator Grant 865459 (“FuncDis3D”) (EdW), Vidi grant from the Netherlands Scientific Organization (NWO, 016.16.316) (EdW), and Veni grant from the Netherlands Scientific Organization (NWO, 016.Veni.181.014) (NQL). The Oncode Institute is partly supported by the KWF Dutch Cancer Society.
Ethics approval and consent to participate
Consent for publication
The current affiliation of Daan Peric-Hupkes (Annogen B.V.) has had no influence on this research. The authors declare no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Additional file 1: Fig S1. LAD border enrichment of epigenetic marks is independent of CTCF orientation and is conserved between cell types. Fig S2. LaminB1 pA-DamID tracks recapitulate DamID data and can be used to profile NL interactions after rapid protein depletion. Fig S3. Effect of protein depletions on the cell cycle. Fig S4. Further analysis of the effect of CTCF depletion on LAD border positioning. Fig S5. Perturbed NL interactions are limited for outwards oriented LAD borders and caused by changes in LaminB1 reads. Fig S6. Protein depletion by AID-tagging induces a partial effect on CTCF detachment within LADs. Fig S7. Quantification of LAD differences after CTCF depletion and cohesin perturbation. Fig S8. Correlation between LAD features. Fig S9. CTCF depletion and cohesin perturbation affect an overlapping gene set enriched for differentiation genes. Fig S10. H3K27me3 depletion does not affect genome-wide NL interactions.
About this article
Cite this article
van Schaik, T., Liu, N.Q., Manzo, S.G. et al. CTCF and cohesin promote focal detachment of DNA from the nuclear lamina. Genome Biol 23, 185 (2022). https://doi.org/10.1186/s13059-022-02754-3
- Nuclear lamina
- Lamina-associated domains
- Acute protein depletion
- Local detachment