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Fig. 2 | Genome Biology

Fig. 2

From: CTCF and cohesin promote focal detachment of DNA from the nuclear lamina

Fig. 2

CTCF and cohesin mediate local detachment at CTCF-marked LAD borders. A Bar plot showing the fraction of LAD borders within 20 kb from a loop anchor as defined by ref. [36] B LaminB1 z-scores along a representative chromosome for all AID-tagged mESC lines without protein depletion (0 h) and following 24 h of IAA-induced protein depletion. Data are binned at 10 kb and averages of n biological replicates. The colored arrows highlight example regions with deviating LaminB1 signal over time and are described in more detail in the main text. C Average LaminB1 z-scores around LAD borders for protein depletion time courses (0 h, 6 h, 24 h) for mESCs with AID-tagged RAD21, WAPL, and a combination of CTCF and WAPL, as described in Fig. 1E. D Quantification of the change in LaminB1 z-score outside LAD borders, as described in Fig. 1F. E Cumulative density profile of LAD border positioning relative to LAD borders defined in the PT clone, as described in Fig. 1G

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