- Open Access
Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins
- Rolen M. Quadros†1,
- Hiromi Miura†2, 3,
- Donald W. Harms1,
- Hisako Akatsuka2, 4,
- Takehito Sato4,
- Tomomi Aida5, 6, 7,
- Ronald Redder8,
- Guy P. Richardson9,
- Yutaka Inagaki3, 10, 11,
- Daisuke Sakai10, 12,
- Shannon M. Buckley13, 15,
- Parthasarathy Seshacharyulu14,
- Surinder K. Batra14, 15,
- Mark A. Behlke16,
- Sarah A. Zeiner16,
- Ashley M. Jacobi16,
- Yayoi Izu17,
- Wallace B. Thoreson18,
- Lisa D. Urness19,
- Suzanne L. Mansour19Email author,
- Masato Ohtsuka2, 3, 10Email author and
- Channabasavaiah B. Gurumurthy1, 20Email author
© The Author(s). 2017
- Received: 3 April 2017
- Accepted: 24 April 2017
- Published: 17 May 2017
Conditional knockout mice and transgenic mice expressing recombinases, reporters, and inducible transcriptional activators are key for many genetic studies and comprise over 90% of mouse models created. Conditional knockout mice are generated using labor-intensive methods of homologous recombination in embryonic stem cells and are available for only ~25% of all mouse genes. Transgenic mice generated by random genomic insertion approaches pose problems of unreliable expression, and thus there is a need for targeted-insertion models. Although CRISPR-based strategies were reported to create conditional and targeted-insertion alleles via one-step delivery of targeting components directly to zygotes, these strategies are quite inefficient.
Here we describe Easi-CRISPR (Efficient additions with ssDNA inserts-CRISPR), a targeting strategy in which long single-stranded DNA donors are injected with pre-assembled crRNA + tracrRNA + Cas9 ribonucleoprotein (ctRNP) complexes into mouse zygotes. We show for over a dozen loci that Easi-CRISPR generates correctly targeted conditional and insertion alleles in 8.5–100% of the resulting live offspring.
Easi-CRISPR solves the major problem of animal genome engineering, namely the inefficiency of targeted DNA cassette insertion. The approach is robust, succeeding for all tested loci. It is versatile, generating both conditional and targeted insertion alleles. Finally, it is highly efficient, as treating an average of only 50 zygotes is sufficient to produce a correctly targeted allele in up to 100% of live offspring. Thus, Easi-CRISPR offers a comprehensive means of building large-scale Cre-LoxP animal resources.
- Homology directed repair
- long ssDNA donors
- CRISPR ribonucleoproteins
- Conditional knockout
- Reporter and recombinase knock-in
Conditional knockout mouse models, in which one or more critical coding exons of a gene are flanked by similarly oriented LoxP sites (i.e., floxed), are among the most useful genetically engineered models in biomedical research. They provide the opportunity to define essential gene functions in both global and tissue-specific contexts [1, 2] and are particularly critical for analyzing genes that have essential functions early in development. Indeed, several large-scale global projects pursued under the umbrella of the International Mouse Phenotyping Consortium (IMPC) set a collective goal of generating a floxed or deletion allele for each mouse gene and to make these alleles readily available to the research community [3, 4]. To date, this goal has been pursued using traditional strategies that rely on homologous recombination (HR) in embryonic stem (ES) cells to deliver targeting cassettes flanked by long regions of homology (~3–10 kb) to the gene of interest , followed by appropriate selection techniques. Correctly targeted ES cells are then introduced into mouse embryos, and the resulting chimeric mice are used to transfer the floxed allele to subsequent generations. The time required to generate floxed mice by the standard method is at least 6 months, even when starting with an ES cell line procured from one of the repositories. Furthermore, only about 25% of mouse genes have been targeted in this way, and the genetic background of ES cells used by the consortium is limited to the C57BL/6 strain, which, though a reference strain, is not ideal for all purposes [6, 7].
CRISPR/Cas9-directed genome editing should, in theory, allow for the more rapid generation of floxed alleles in any chosen genetic background, because the editing components can be delivered directly to single-cell mouse zygotes of any strain. Indeed, within months of the first demonstration of CRISPR/Cas9 genome editing to produce small gene disruptions in mammalian cells [8, 9], a proof-of-concept study showed that conditional knockout mice could be generated by homology-directed repair (HDR) following injection of mouse zygotes with five components: two separate single guide RNAs (sgRNAs) targeted to sequences flanking an exon of interest; two single-stranded oligodeoxynucleotide (ssODN) donors, each containing a LoxP site flanked by short (40–80 bases) arms homologous to the desired insertion site; and Cas9 mRNA. Using this method, the authors found that Mecp2 was correctly floxed in 16% of the embryos/mice derived after transfer of injected zygotes . To date, however, only two other reports have been published showing that floxed mice can be generated using this approach. Bishop et al.  and Miano et al.  reported efficiencies of LoxP integration of 2–5% and identified some of the reasons for its poor success. A recent news article in Science reported anecdotal evidence that this method has been unsuccessful at many loci, and that cases of successful CRISPR-directed floxing had efficiencies of only 1 or 2% . A major factor limiting the targeting efficiency of this approach is the complex set of modifications that the targeting components can generate in addition to the desired insertion of two LoxP sites located in cis. These include single LoxP insertions, double LoxP insertions located in trans, and a variety of deletions resulting from non-homologous end-joining (NHEJ), all of which may vary in a locus-dependent manner [10, 11]. Thus, this appealingly simple and rapid two-ssODN donor approach is not robust enough for routine generation of floxed alleles.
As an alternative to short ssODN donors, insertions of longer sequences (floxed exons or coding sequences) have been attempted using double-stranded DNA (dsDNA) donors with homology arms of at least 0.5–1 kb. Compared with ssODN donors, the insertion efficiency of dsDNA donors is often poor [10, 14–16]. For example, an IMPC study showed that classic HR-mediated cassette insertion could be achieved directly in zygotes by creating two nicks near the target site using Cas9 nickase and co-injecting a floxed donor cassette of dsDNA with homology arms of ~1.9 kb . However, only one out of thirteen pups born contained the desired allele, and this approach has not been used routinely.
Other strategies for increasing the efficiency of CRISPR/Cas9 genome editing include inhibition of NHEJ or enhancement of HDR through chemical treatments [18, 19]. These approaches, however, are based on perturbation of DNA repair processes and may be toxic . Additional strategies include the use of circular donors with built-in artificial guide sequences that are linearized inside the cell/embryo, wherein donors are inserted at the genomic Cas9 cleavage site by cellular ligases [21–23]. These targeting designs include either micro-homology ends between the cleaved ends of the genomic DNA and donor DNA, or ssODNs that bind to the two free ends so that precise fusion occurs between the donor and genomic DNAs. Although these latter strategies offer better alternatives to those that perturb DNA repair, they too have limitations, including low-to-moderate efficiencies and the need for custom design of donor plasmids for each target site. Due to the poor efficiency of direct zygote injections, some groups have also tried to develop CRISPR/Cas9-based strategies for creating knock-ins via ES cell targeting [20, 24, 25]. Although these proved feasible, they are neither efficient nor robust enough for routine application.
Because short ssODN donors are inserted efficiently at Cas9 cleavage sites through an HDR pathway, we reasoned that this repair mechanism might be exploited to deliver longer cargo if the length of the single-stranded DNA (ssDNA) could be extended. Based on our experience with using ssDNA donors and an sgRNA to insert ~400-base fragments into the mouse genome with high efficiency when assayed at embryonic stages , we asked whether longer ssDNA donors and two guide RNAs could be used to generate mice with floxed exons. Here, we demonstrate that long ssDNA donors with short homology arms generate conditional knockout mice at high efficiency when using pre-assembled crRNA + tracrRNA + Cas9 ribonucleoprotein (ctRNP) complexes containing two guide RNAs. We also show that knock-ins of reporter, recombinase, and transcriptional effector genes can be generated at similar efficiencies, by providing long ssDNA donors with ctRNPs that contain one guide RNA. Our method, called Easi-CRISPR (Efficient additions with ssDNA inserts-CRISPR), is robust and, having been tested at more than a dozen loci (creating seven floxed and six knock-in alleles), is also highly generalizable. Easi-CRISPR thus provides a comprehensive solution to the challenges of generating both necessary components (floxed and Cre alleles) for conditional gene ablation in mice, as well as enabling rapid development of numerous other desired alleles.
Efficient generation of floxed alleles using long ssDNA donors
As a test case, we selected Pitx1 and generated a 1046-base ssDNA donor containing a floxed version of exon 2, flanked by 93- and 91-base left and right homology arms, respectively. Two guide RNAs (sgRNAs) were designed to cut the genome immediately adjacent to each homology arm (Additional file 1: Figure S1a). We injected the ssDNA donor with Cas9 mRNA and the two sgRNAs into mouse zygotes following standard CRISPR genome engineering protocols . Genotyping of the resulting live offspring, using three sets of PCR reactions specific for targeted insertion of each LoxP site and for the entire floxed exon, revealed that one out of eight (13%) carried a correctly floxed allele (Additional file 1: Figure S1b–g). Three other pups had partial insertions of the donor cassette: two contained only a single targeted LoxP site and one contained both LoxP insertions, but they were located on separate alleles (in trans).
Microinjection data for floxed allele generation at seven loci
ssDNA length Left Arm-Cassette-Right Arm (bases) [source of ssDNA]
Live-born animals (percentage of transferred zygotes)
Targeted animals (%)a
Pitx1-exon 2 floxed
93 + 862 + 91
Ambra1-exon 4 floxed
96 + 589 + 103
Col12a1-exon 2 floxed
55 + 527 + 55
Ubr5-exon 58 floxed
78 + 535 + 86
Syt1-exon 6 floxed
75 + 635 + 75
Syt9-exon 3 floxed
87 + 893 + 68
PPP2r2a-exon 3 floxed
95 + 619 + 84
To directly compare Easi-CRISPR with the previously described method for generating floxed alleles , we targeted the same Pitx1 exon using two guides and two short ssODN donors containing the LoxP sites (Additional file 1: Figure S3a). We prepared the Pitx1 ctRNP exactly as described above and injected it, together with the two ssODN donors, into 66 zygotes, from which 18 animals were born. Genotyping showed that many animals carried a single LoxP site (three had only the 5′ LoxP and three had only the 3′ LoxP). Only one of the 18 animals contained both LoxP sites on the same allele (in cis; Additional file 1: Figures S3b, c, lane 2). However, the sequence of the distal LoxP site contained a mutation (Additional file 1: Figure S3d), and therefore this animal would not be useful for conditional deletion of Pitx1; similar unwanted mutations, in LoxP sites, were reported previously for another locus . Of note, even the genomes that had single LoxP insertions also contained various types of deletions (evident by differently sized PCR products; Additional file 1: Figure S3c, lanes 5, 8, 14, and16). These results clearly confirm that although various types of insertion events can occur when using the two-ssODN donor method, it is quite challenging to identify and/or obtain correctly targeted animals. These observations are similar to those made by others [11, 12].
In summary, for all seven genes combined, genotyping of 46 G0 pups showed that 20 (43%) contained at least one correctly floxed allele, with an efficiency ranging from 8.5–100% at different loci. The fidelity of the insertions and correct fusions was confirmed by sequencing (Additional file 1: Figures S4–S10). Of the 20 founders with correctly floxed exons, two contained point mutations in the inserted regions (one each for Pitx1 and Ambra1) that may have derived from enzymatic misincorporation during preparation of the ssDNA donor templates. Nevertheless, such mutations did not affect the overall goal of generating floxed mice because we obtained at least one founder with a correct insertion for each gene. Moreover, even the founders with mutations are potentially useful because the mutations were located in intronic sites that are less likely to affect gene function.
Efficient generation of knock-in alleles using long ssDNA donors
Microinjection data for knock-in allele generation at six loci
ssDNA length Left Arm-Cassette-Right Arm (bases) [source of ssDNA]
Live-born animals (percentage of transferred zygotes)
Targeted animals (%)a
105 + 1368 + 98
99 + 1368 + 72
85 + 1368 + 96
96 + 1220 + 98
60 + 782 + 60
60 + 779 + 60
Easi-CRISPR founders transmit their modified alleles to offspring, which show the expected phenotypes
Development of a high efficiency method for creating conditional and insertion alleles
Even though the CRISPR system has led to many paradigm shifts in animal transgenesis [31–33] and is routinely used to generate mice with small insertions and deletions, until now there have been no simple strategies for efficient and targeted insertion of long sequences via direct zygote microinjection of CRISPR components. There has been an intensive effort made by the community during the past 3 years to use CRISPR-based strategies for developing floxed models through zygote injections [11, 12, 17], and also through ES cell targeting strategies [20, 24, 25]. The primary objective of this work was to develop a CRISPR targeting strategy suitable for both high- and low-throughput generation of floxed animal models. The criteria we set for the new strategy were that it should be: (1) easy to design and build floxed-donor DNA cassettes, (2) compatible with zygote injections, (3) very efficient, and (4) generalizable to many loci.
To this end, we extended our previous finding that ~400-base ssDNAs serve as efficient donors for HDR at single Cas9 cleavage sites , by first showing that long ssDNA donors with short homology arms can be used to replace a gene segment between two Cas9 cleavage sites, a critical technical necessity for generating floxed alleles. Given the multitude of potential undesired products that are possible from the NHEJ repair pathway acting at two Cas9 cleavage sites, the high frequency of recovering correctly floxed alleles at seven different loci by using Easi-CRISPR (8.5–100%) was surprising, as previously described strategies reached a maximum of 16% efficiency [10, 11]. Of note, a floxed allele for Col12a1, one of the loci targeted here by Easi-CRISPR, was recently developed by using a strategy called PITCh (Precise Integration into Target Chromosome) . The targeting of Col12a1 by PITCh used the same set of ctRNP complexes that were used in Easi-CRISPR (described above) but the difference between the two methods was in the donor DNA format, unlike the ssDNA donor used in Easi-CRISPR, the PITCh system used a dsDNA donor. The PITCh approach required 265 zygotes whereas Easi-CRISPR used only 105 zygotes, and the PITCh approach produced 33% correctly targeted pups, whereas Easi-CRISPR (using ssDNA donors) produced 100% correctly targeted pups. Of note, the PITCh experiment included the Exo1 nuclease, an enhancer of targeted insertion . In its absence, the efficiency would likely have been lower than 33%.
While developing our method, an IMPC study investigated whether floxed gene-targeting vectors that had already been created for ES cell targeting could be modified and used as dsDNA donors for zygote injections . Although this study showed the feasibility of the approach for one gene (six targeted out of 17 live born, 35% efficiency; or one targeted out of two live born, 50% efficiency), when the same strategy was applied to two other genes, only one of the two genes yielded a floxed allele (one targeted out of nine pups for one gene, and zero out of nine pups for the second gene: 11 and 0% efficiency, respectively). In comparison, Easi-CRISPR offers better options for several reasons. First, the complex gene-targeting vectors must be modified in the IMPC approach before they can be used for zygote injections, whereas Easi-CRISPR donor designs are simple and cassettes can be synthesized rapidly by commercial custom gene synthesis services. Second, the efficiency of the IMPC method is lower for creating conditional alleles (0 to 50%), compared to Easi-CRISPR, which has an efficiency of 8.5–100%.
In addition to floxed alleles, knock-ins of coding sequences for recombinases and reporter genes are also critical tools for Cre-LoxP-based mouse molecular genetics, and they serve many additional purposes in biomedical research. Furthermore, in some studies, gene expression or site-specific recombination is achieved by using inducible systems, such as those involving the tetracycline-induced transactivators and repressors . By successfully targeting insertions to six loci we demonstrate that Easi-CRISPR is suitable for generating all such knock-in models. Therefore, Easi-CRISPR offers a comprehensive solution to the Cre-LoxP mouse genetics system because it also allows creation of reporter/recombinase/transcriptional effector knock-ins, not just conditional alleles.
Some potential limitations of our method are: (1) Targeting single exon genes or genes containing unusually repetitive sequences. This limitation, however, is applicable to any gene targeting approach. (2) Targeting insertions longer than 2 kb. Although many types of commonly used mouse models such as floxed, Cre-, rtTA-, and reporter- knock-in alleles can be created using ssDNA donors of 1 to 2 kb long, expanding the synthesis capability of donors to several kilobases long would enable creation of a wider range of animal models. This will require technical improvements that extend the lengths of ssDNA donors. (3) Variability of cleavage efficiencies of guides (discussed below) can influence insertion efficiencies at different loci. This issue is inherent to any CRISPR-based targeting approach. We are confident that future advances to, or further modifications of, Easi-CRISPR will address such potential limitations.
Easi-CRISPR is highly robust and generalizable
Easi-CRISPR is robust because one or more correctly targeted animals can be generated by injecting as few as 50 zygotes per gene-targeting project (13 targeting projects were completed using 639 zygotes). The method is also highly generalizable because it has worked for over a dozen loci. We noted a wide range in the frequency of insertions for different loci (8.5–100%). There are several possible explanations for this finding. First, we think that a major factor contributing to the differences in insertion frequencies may arise from the differences in cleavage efficiency of guides. A recent report described a systematic analysis of guide features and identified the parameters that contribute to variability in cleavage efficiency. They found that cleavage depended on many factors, including nucleotide sequences at both PAM-distal and PAM-proximal regions of the sgRNA, the genomic context of the targeted DNA, the GC percentage, and the secondary structure of sgRNA [37, 38]. It is possible, therefore, that some guides may not work and trying alternative guides for those loci may be necessary . Finding a suitable, high efficiency, guide can be a critical factor for targeting experiments in which insertion of a fusion cassette at a specific codon in the genome is required and good guides are not available for the target site. In contrast, guide location is not a major factor in designs for floxed alleles because the position of LoxP sites in introns is flexible. For successful floxing, however, both guides need to be equally efficient at directing cleavage; if one site is cleaved less efficiently than the other, the overall targeting efficiency may be lower. This situation may have occurred with our Pitx1 and Syt1 targeting in which only one of the two LoxP sites was inserted in some animals (40% of Pitx1 founders; 25% of Syt1 founders). We suggest that these partial insertions occurred because the second guide may not have cleaved the genome in those zygotes. A second explanation for the variability in insertion efficiencies may be differences in the genomic loci themselves. It was proposed previously that HDR varies widely from locus to locus [40–42]. A third explanation could be the inherent variability in the experimental steps of animal transgenesis, such as embryo isolation and ex vivo handling for microinjection, variability in the embryo transfer procedure after microinjection, etc. Despite these potential limitations, the method presented here efficiently generates at least one correctly targeted animal for each locus and, frequently, most of the animals born contain the targeted allele.
Easi-CRISPR will be simple to adapt for both low-throughput and high-throughput labs
Microinjection of mouse zygotes is a standard technique and with the exception of one locus (Col12a1; 3.8%), the birth rates of Easi-CRISPR manipulated zygotes were 9–31%, which is similar to that observed with conventional pronuclear injections . Although these birth rates may seem low for some loci, transgenic experiments involve many complicated steps, variation in which affects birth rates. These include the quality of microinjection reagents, the many steps of assisted reproduction, animal husbandry conditions, and, finally, the mothering ability of the recipient females. Very importantly, the proficiency of technicians involved can be another major factor. For example, the extent of trauma caused to the embryos by the volume of liquid injected and the success of embryo transfers to the oviducts can vary from technician to technician. Consequently, most transgenic core labs that rely on currently available HDR strategies typically inject about 200–300 or more zygotes to generate knock-in models. Even so, in many cases, these projects are unsuccessful. Despite the factors discussed above, Easi-CRISPR clearly stands out as we have thus far successfully created founders for 13 loci by injecting only 639 zygotes. The majority of these Easi-CRISPR targeting projects were completed by injecting only about 40–60 zygotes. Of note, the founders for these alleles were created at three independent facilities by different technical staff and all projects were successful. Thus, Easi-CRISPR should be easily adaptable to most low- and high-throughput applications.
Mechanistic thoughts about the high efficiency of Easi-CRISPR
Our results suggest that long ssDNAs are key to achieving high HDR efficiency in CRISPR genome editing. The other most significant factor contributing to the high efficiency of Easi-CRISPR could be the ctRNP delivery of the targeting components. Our experiment directly comparing Cas9-mRNA/sgRNA injection versus ctRNP (crRNA + tracrRNA + Cas9 protein) for floxing Pitx1 showed that ctRNP delivery was about three-fold more efficient than when all components were delivered as RNA. A similar observation was reported by Aida et al. , who compared the sgRNA/Cas9 mRNA, sgRNP, and ctRNP platforms programmed with dsDNA donors and concluded that ctRNP (referred to as cloning-free CRISPR/Cas) was the most efficient. A recent study of sgRNP electroporation also indicates its superior performance over sgRNA/mRNA delivery . Taken together, we conclude that crRNA + tracrRNA (instead of sgRNA), Cas9 protein (instead of Cas9 mRNA), and long ssDNA donors (instead of dsDNAs) are central to obtaining consistently higher success in CRISPR animal genome engineering. The high efficiency of Easi-CRISPR could also be combined with electroporation-based delivery methods such as GONAD [45, 46], TAKE , and SLENDR  to generate floxed or knock-in mice.
The unexpected observation that long ssDNA donors drive high insertion efficiencies leads to the question of why there is such a large difference between the targeting efficiencies of ssDNA versus dsDNA donors. Among different HDR types, classic homologous recombination (HR) uses dsDNA as a donor, while some recently identified processes, including single strand annealing (SSA)  or micro-homology mediated end joining (MMEJ; also known as Alt-EJ) , rely on the availability of annealable-partner sequences within the non-recessed ends themselves. Considering the properties of our donors, we speculate that the proteins responsible for either SSA or MMEJ may be involved in ssDNA donor-mediated repair. Of these two, MMEJ factors are less likely because this mechanism relies on very short homologies [50, 51]; whereas SSA operates with arms typically longer than 30 bases (the arms in our ssDNA donors are 55–105 bases) . It was recently proposed that the MMEJ mechanism applies when the donors for CRISPR editing contain 5–25 base homology arms, although the protein factors involved in this repair process need to be validated. Many protein factors involved in various types of HDR pathways have been characterized [49, 51, 52] and a systematic analysis of Easi-CRISPR frequencies, in the absence of some of those factors in mouse embryos, will help delineate the molecular mechanisms involved.
Other potential applications of Easi-CRISPR
Our results suggest that it is possible to create gene-replacement models, as demonstrated by our finding that two cleavages can be used to take out a target exon and replace it with a floxed exon cassette. Thus, Easi-CRISPR will also be suitable for generating other types of DNA replacements, such as (1) a set of point mutations spread across a region (e.g., up to 1–2 kb long that can be efficiently inserted), (2) testing regulatory sequences, and (3) replacing short stretches of gene segments or coding sequences from other species (e.g., creating humanized mice). In addition, Easi-CRISPR could be used to modify existing knock-in alleles, for example, by inserting an frt-stop-frt cassette into an existing lox-stop-lox-controlled gene to enable dual recombinase control of that gene without having to start from a wild-type allele.
Because of the availability of numerous genome engineering tools developed during the past four decades, the mouse has become the main species used to model human genetic pathophysiology. However, there are many cases in which mouse models do not recapitulate human disease and other species are preferred. Easi-CRISPR, with its simple design requirements and high efficiency, may provide the solution to engineering the genomes of medically relevant laboratory animals as well as livestock species for which zygote injections can be performed successfully. For example, there is a particular need for rat models [53, 54]. The community has begun exploring CRISPR strategies for generating Cre-LoxP rat models [15, 55] and many commercial service providers have initiated rat genome modification services . We anticipate that because of its numerous benefits, including simplicity of design, high efficiency, effectiveness for many genes, and suitability for both low- and high-throughput laboratories, Easi-CRISPR will serve as an effective means of rapidly building mouse Cre-LoxP resources, and for building similar resources for rat and other models in the future.
Conditional knockout and transgenic/knock-in models expressing reporters or recombinases together constitute over 90% of genetically engineered mouse models created routinely. Although it was previously claimed that the CRISPR/Cas9 system could be readily used for developing such models, it has proven to be highly challenging because the insertion of foreign DNA cassettes at Cas9 cleavage sites is inefficient. The Easi-CRISPR strategy we describe here uses simplified CRISPR tools; long ssDNA donors and ctRNPs, and allows the insertion of DNA cassettes into genomes with a very high efficiency. The method has been used at over a dozen loci revealing robustness, high efficiency and, moreover, versatility as it can create conditional as well as recombinase, reporter, and transcriptional effector knock-in alleles. The method is also easily adaptable to both low- and high-throughput genome engineering applications. Easi-CRISPR therefore solves a major challenge in the CRISPR animal genome engineering field and offers a comprehensive system for building large-scale Cre-LoxP animal resources.
CRISPR guide RNAs were designed using CRISPR.mit.edu, or CHOPCHOP, and were used as annealed two-part synthetic crRNA and tracrRNA molecules for all genes (Alt-RTM CRISPR guide RNAs, Integrated DNA Technologies, Inc. (IDT), Coralville, IA, USA and Genome Craft Type CT, FASMAC, Kanagawa, Japan), and as sgRNAs for Pitx1. Cas9 mRNA (used for the Pitx1 floxing experiment; Additional file 1: Figure S1) was prepared using the pBGK plasmid as described previously . The sgRNAs (used for the Pitx1 floxing experiment; Additional file 1: Figure S1) were synthesized as described previously . The plasmid was linearized with XbaI and used as the template for in vitro transcription using the mMESSAGE mMACHINE T7 ULTRA kit (Ambion, AM 1345). Recombinant Cas9 protein employed for RNP injections was the Alt-R™ S.p. Cas9 Nuclease 3NLS (IDT), or from New England Biolabs, or FASMAC. dsDNA templates for floxing experiments (containing the homology arms and the floxed exon sequences) for producing ssDNA donors were custom synthetic genes made by Life Technologies or IDT (for floxing experiments) and knock-in cassettes were amplified using long primers to add homology arms. The ssDNA HDR donors were prepared from these cloned dsDNA templates either using the IvTRT method as described previously  or obtained from IDT (Megamer™ single-stranded Gene Fragments). Both IvTRT and IDT Megamer™ ssDNA preps showed comparable HDR efficiencies. Although the two different versions of ssDNAs have not been tested on the same genetic locus, we do not anticipate any performance differences between the two sources (Tables 1 and 2).
Preparation of CRISPR injection mixes
The ctRNP mixes were prepared as follows. Lyophilized crRNA and tracrRNA (commercially procured) were re-suspended in microinjection buffer (TrisHCl 10 mM, pH 7.5, EDTA 0.1 mM). Five micrograms of crRNA (5 μl of 1 μg/μl) and 10 μg of tracrRNA (10 μl of 1 μg/μl) were combined in a PCR tube and were annealed in a thermocycler (95 °C for 5 min followed by ramp down to 25 °C at 5 °C/min). The annealed crRNA and tracrRNA (also known as guide RNA) were diluted in microinjection buffer and mixed with Cas9 protein to obtain ctRNP complexes . The final concentrations of components in ctRNP preparations were 5–20 ng/μl of guide RNA (if two guides were used, each guide was at 5–20 ng/μl) and 5–50 ng/μl of Cas9 protein. The ssDNA donors were mixed with ctRNP complexes at 5–10 ng/mix and the final injection mixes were passed through Millipore Centrifugal Filter units (UFC30VV25, EMD Millipore, Billerica, MA, USA) and spun at 21,000 g for 5 min at room temperature.
Microinjection of one-cell embryos
All animal experiments performed were approved by the respective institutional IACUC protocols. C57BL/6 mice at 3–4 weeks of age (Charles River Laboratories, Wilmington, MA, USA or CLEA, Tokyo, Japan) were superovulated by intraperitoneal injection of 5 IU pregnant mare serum gonadotropin, followed 48 h later by injection of 5 IU human chorionic gonadotropin (both hormones from National Hormone & Peptide Program, Torrance, CA, USA). Mouse zygotes were obtained by mating C57BL/6 stud males with superovulated C57BL/6 females. One-cell stage fertilized mouse embryos were injected with 5–50 ng/μl Cas9 protein (or 10 ng/μl of Cas9 mRNA; for Pitx1 locus), 5-20 ng/μl of annealed crRNA and tracrRNA (or 10 ng/μl of each sgRNA; for Pitx1 locus) and 5–10 ng/μl of ssDNA. Microinjections and mouse transgenesis experiments were performed as described previously .
Mouse genomic DNA extraction, genotyping, and sequencing
Mouse genomic DNA was extracted from toe or ear samples using the Qiagen Gentra Puregene Tissue Kit (Qiagen Sciences, Maryland, USA) or Allele-In-One Mouse Tail Direct Lysis Buffer (KURABO, Osaka, Japan). Primers were designed to amplify the correctly targeted junctions. Genomic DNA was subjected to flanking primer PCR and internal (donor oligo-specific) and external primer PCR. The primer sequences for all 13 genes are listed in Additional file 1: Table S1. PCR reactions were performed using the Go Taq Promega Hot Start green mix (Promega, Madison, WI, USA) or PrimeSTAR HS DNA Polymerase (TaKaRa, Shiga, Japan). The amplicons were separated on a 1–3% agarose gel. The gel-purified amplicons were subjected to sequencing using one of the PCR primers and/or internal primers. In some cases, PCR products were cloned into TA (Life Technologies, catalog number K2020-20) vectors before sequencing.
FlpO activity assay
A homozygous FLP reporter mouse, B6.Cg-Gt(ROSA)26Sor tm1.3(CAG-tdTomato,-EGFP)Pjen /J (JAX Stock #026932) , was crossed with the Fgf8 -P2A-FlpO #4. Whole cochleae were dissected from P1–P2 pups, cut along Reissner’s membrane to expose the surface of the sensory epithelium, and fixed overnight at 4 °C in 4% paraformaldehyde in PBS. The cochleae were stained with Alexa488-phalloidin (Invitrogen) diluted 1:1500 in PBS containing 0.1% Triton X-100 for 15 min, and then mounted in Fluoromount-G (SouthernBiotech) on microscope slides. Cochleae were imaged on an Axioskop (Zeiss) with epifluorescent illumination and photographed with an Infinity 3-6UR (Lumenera) digital camera. Green and red channels were overlaid using Photoshop CS6 (Adobe).
Quantification and statistical analysis
The robustness of the genome-editing method developed in this work was tested at 13 independent genomic loci. Each locus-specific experiment was performed by injecting zygotes to generate founders until at least one correctly targeted founder animal was obtained. Based on this criterion, all the 13 loci tried yielded targeted animals (i.e., 100% success rate). The number of zygotes injected ranged from 19 to 105 per locus with an average of 50 zygotes injected per locus to successfully complete a project (to obtain at least one correctly targeted animal). The overall efficiency of individual projects was calculated by the percentage of correctly targeted animals among the total number of live born animals, which ranged from 8.5 to 100%. The possible reasons of variability across different genomic loci are included in the discussion section.
We thank Harumi Ishikubo, Takako Usami, and the genome editing facility at laboratory of recombinant animals, MRI, TMDU, and Y. Wada (FASMAC) for providing technical assistance and materials. We thank Adrian Koesters of UNMC for her editorial contributions and Joseph M. Miano, University of Rochester for his helpful comments on the manuscript. We also gratefully acknowledge the contribution of the staff of the Support Center for Medical Research and Education, Tokai University, for sequencing and microinjection.
This work was supported in part by an Institutional Development Award (PI: Shelley Smith) P20GM103471 (to CBG, RMQ, DWH, and RR), by NIGMS 1P30GM110768-01 and P30CA036727 to RR (as part of support to University of Nebraska DNA Sequencing Core), by NIH R21DC014779 (to SLM) and by KAKENHI (26830131, 16 K07085, Comprehensive Brain Science Network and Adaptive Circuit Shift) from JSPS and MEXT, grants from Nakatani Foundation, SENSHIN Medical Research Foundation, Mochida Memorial Foundation, Takeda Science Foundation, MRI, and CNSI/NINS (BS281001) to TA, KAKENHI 15 K19988 from JSPS and grants from the Nakatomi Foundation, the Ichiro, Kanehara Foundation, and Takeda Science Foundation to Y. Izu, and by Research and Study Project of Tokai University General Research Organization, 2016–2017 Tokai University School of Medicine Project Research to MO, and by Grant-in-Aid for Young Scientists (B) (16 K18821) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) to HM, and by MEXT-Supported Program for the Strategic Research Foundation at Private Universities 2015-2019 to Tokai University, and the Wellcome Trust (grant 087377) to GPR, Fred and Pamela Buffet Cancer Center’s ACS Institutional Research Grant to SMB, by NIH grant EY10542 and Senior Scientific Investigator Award Research from Research to Prevent Blindness (to WBT).
Availability of data and materials
CBG, GPR, MO, and SM conceived this study, CBG, RMQ, GPR, MO, HM, TA, SMB, PS, SKB, MAB, Y. Inagaki , TS, WBT, DS, and SLM designed the experiments, CBG, RMQ, HM, DWH, TA, SAZ, RR, HA, AMJ, Y. Izu, and LDU performed experiments. CBG, RMQ, MO, GPR, and SLM wrote the manuscript with input from other authors. All authors read and approved the final manuscript.
CBG, MO, and HM have filed patent application relating to the work described in this manuscript on international application number PCT/US2016/035660 filed June 3, 2016 (DNA editing using single stranded DNA). MAB, AMJ, and SAZ are employed by Integrated DNA Technologies, Inc. (IDT), which offers oligonucleotides for sale similar to some of the compounds described in the manuscript. IDT is, however, not a publicly traded company and these authors do not personally own any shares/equity in IDT.
All animal experiments performed were approved by the respective institutional IACUC protocols (University of Nebraska Medical center; 13–097, Tokyo Medical and Dental University; 0160341A, Tokai University School of Medicine; 165009, University of Utah; 14–04018).
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