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Fig. 4 | Genome Biology

Fig. 4

From: Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins

Fig. 4

Germ line transmission of founder alleles generated using Easi-CRISPR. a–f Genotyping of offspring from two founders each for the Pitx1 and Ambra1 conditional alleles (a, b) and one founder each of the Fgf8, Slc26a5, Mmp9, and Mmp13 knock-in alleles (c–f) showing germ line transmission from all of these founders. As expected, all the pups from the Fgf8 founder contain a targeted allele because the founder is biallelic (c)

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Genome Biology

ISSN: 1474-760X

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