Fig. 5

Easi-CRISPR alleles perform as intended. Conditional alleles show the expected pattern of Cre-mediated deletion. a Genotyping of lymphocyte DNA isolated from a litter produced by mating the Ambra1 floxed founder 1 (Fig. 2g, lane 1) with a CD4 Cre strain. Offspring carrying both the floxed and Cre alleles (first three lanes) show the expected PCR amplicons. wt wild-type control sample, M 100-base pair marker. b Sequencing of a deletion allele showing Cre recombination (see Additional file 1: Figure S5 for comparing this sequence with the floxed allele sequence). c FGF8-P2A-FlpO activates a FLP-dependent tdTomato reporter in inner hair cells. A surface preparation of the cochlear epithelium isolated from a P1-P2 Fgf8 ^P2A-FlpO/+ ;Rosa26 ^RC::RFLG/+ animal was stained with Alexa488-phalloidin (green). Native tdTomato fluorescence (red) is evident in most inner hair cells (i), but not in outer hair cells (o)