Skip to main content

Advertisement

We’d like to understand how you use our websites in order to improve them. Register your interest.

Green flies

In the December 18 Proceedings of the National Academy of Sciences, Morin et al. describe a gene-trap strategy that generates green fluorescent protein (GFP) fusions and allows the study of protein distribution and subcellular localization in living flies (Proc Natl Acad Sci USA 2001, 98:15050-15055). They created a protein-trap transposon (PTT), a P element containing an artificial exon encoding GFP and flanked by splice acceptor and donor sequences. They derived over 600 fluorescent Drosophila lines and observed fusion proteins localized in a range of cellular organelles. Characterization of several of these revealed that in most cases splicing occurred correctly and fusions recapitulated endogenous expression of the trapped gene. Over 40% of characterized lines correspond to genes that were not predicted by the Drosophila Genome Project.

References

  1. 1.

    Proceedings of the National Academy of Sciences, [http://www.pnas.org]

  2. 2.

    Green fluorescent protein as a marker for gene expression.

Download references

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Weitzman, J.B. Green flies. Genome Biol 2, spotlight-20011219-01 (2001). https://doi.org/10.1186/gb-spotlight-20011219-01

Download citation

Keywords

  • Fusion Protein
  • Green Fluorescent Protein
  • Fluorescent Protein
  • Subcellular Localization
  • Genome Project