Skip to main content

Green flies

In the December 18 Proceedings of the National Academy of Sciences, Morin et al. describe a gene-trap strategy that generates green fluorescent protein (GFP) fusions and allows the study of protein distribution and subcellular localization in living flies (Proc Natl Acad Sci USA 2001, 98:15050-15055). They created a protein-trap transposon (PTT), a P element containing an artificial exon encoding GFP and flanked by splice acceptor and donor sequences. They derived over 600 fluorescent Drosophila lines and observed fusion proteins localized in a range of cellular organelles. Characterization of several of these revealed that in most cases splicing occurred correctly and fusions recapitulated endogenous expression of the trapped gene. Over 40% of characterized lines correspond to genes that were not predicted by the Drosophila Genome Project.

References

  1. 1.

    Proceedings of the National Academy of Sciences, [http://www.pnas.org]

  2. 2.

    Green fluorescent protein as a marker for gene expression.

Download references

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Weitzman, J.B. Green flies. Genome Biol 2, spotlight-20011219-01 (2001). https://doi.org/10.1186/gb-spotlight-20011219-01

Download citation

Keywords

  • Fusion Protein
  • Green Fluorescent Protein
  • Fluorescent Protein
  • Subcellular Localization
  • Genome Project