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Open Access

Viral discovery

  • Jonathan B Weitzman
Genome Biology20012:spotlight-20010927-01

https://doi.org/10.1186/gb-spotlight-20010927-01

Published: 27 September 2001

The isolation of novel viral genomes from serum or plasma samples presents a significant technical challenge. In the September 25 Proceedings of the National Academy of Sciences, Tobias Allander and colleagues at the National Institute of Allergy and Infectious Diseases, Bethesda, USA, describe a sensitive method for identifying viruses in serum samples (Proc Natl Acad Sci USA 2001, 98:11609-11614). The method is based on the fact that viral genomes are generally protected from DNase degradation by protein capsids and lipid envelopes. Allander et al. developed a technique using DNase treatment of serum followed by nucleic acid extraction, restriction enzyme digestion and sequence-independent single primer amplification (SISPA). This methodology, that they named DNase-SISPA, can detect viruses at titres of less that 106 viral genome equivalents per millilitre. Allander et al. applied the DNase-SISPA technique to clone two new bovine parvoviruses from bovine serum.

References

  1. Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genomeGoogle Scholar
  2. Proceedings of the National Academy of Sciences , [http://www.pnas.org]
  3. National Institute of Allergy and Infectious Diseases , [http://www.niaid.nih.gov]
  4. Sequence-independent, single-primer amplification (SISPA) of complex DNA populations.Google Scholar

Copyright

© BioMed Central Ltd 2001

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