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Genome Biology volume 2, Article number: spotlight-20010322-01 (2001)
Members of the myconcogene family are often amplified or mutated in human tumours. In the March 15 EMBO Journal, Boon et al. described the use of a neuroblastoma cell line with an inducible N-myc allele to identify genes regulated by N-myc (EMBO Journal 2001, 20:1383-1393). They performed serial analysis of gene expression (SAGE) to detect over a hundred genes up-regulated upon N-myc expression. The target genes included over 50 encoding ribosomal proteins, as well as key genes in rRNA maturation and ribosome assembly. Boon et al. also found a 45% higher rRNA content in cells expressing N-myc. Many of the identified N-myc target genes are also up-regulated in neuroblastomas with N-myc amplifications, and about 40% are also upregulated by the related c-myc transcription factor. Induction of the protein synthesis machinery by myc oncogenes may explain their role in regulating cell size.
Function of the c-Myc oncogenic transcription factor.
EMBO Journal, [http://www.emboj.org]
Conditional expression of N-myc in human neuroblastoma cells increases expression of alpha-prothymosin and ornithine decarboxylase and accelerates progression into S-phase early after mitogenic stimulation of quiescent cells.
Serial analysis of gene expression.
c-Myc enhances protein synthesis and cell size during B lymphocyte development.
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Weitzman, J.B. N-myctargets. Genome Biol 2, spotlight-20010322-01 (2001). https://doi.org/10.1186/gb-spotlight-20010322-01