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Breeding a better vector
Genome Biology volume 1, Article number: spotlight-20000807-01 (2000)
DNA shuffling (also called molecular breeding) generates variation by random fragmentation of a cloned gene followed by reassembly of the fragments in a self-priming polymerase reaction. The result is a recombination of overlapping fragments that have different mutations or come from different, naturally occurring homologous genes. In the August Nature Genetics Soong et al. apply this technique to a pool of six different murine leukemia virus envelope sequences to derive a new virus that can, unlike its parents, infect Chinese Hamster Ovary (CHOK1) cells (Nat. Gen. 2000, 25:436-439). Similar selections on clinically relevant cell types may yield improved vectors for gene therapy.
Rapid evolution of a protein in vitro by DNA shuffling.
DNA shuffling of a family of genes from diverse species accelerates directed evolution.
Nature genetics, [http://www.nature.com/ng/]
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Wells, W. Breeding a better vector. Genome Biol 1, spotlight-20000807-01 (2000). https://doi.org/10.1186/gb-spotlight-20000807-01
- Gene Therapy
- Homologous Gene
- Chinese Hamster Ovary
- Leukemia Virus