- Open Access
m:Explorer: multinomial regression models reveal positive and negative regulators of longevity in yeast quiescence
© Reimand et al.; licensee BioMed Central Ltd. 2012
Received: 24 February 2012
Accepted: 29 May 2012
Published: 21 June 2012
We developed m:Explorer for identifying process-specific transcription factors (TFs) from multiple genome-wide sources, including transcriptome, DNA-binding and chromatin data. m:Explorer robustly outperforms similar techniques in finding cell cycle TFs in Saccharomyces cerevisiae. We predicted and experimentally tested regulators of quiescence (G0), a model of ageing, over a six-week time-course. We validated nine of top-12 predictions as novel G0 TFs, including Δmga2, Δcst6, Δbas1 with higher viability and G0-essential TFs Tup1, Swi3. Pathway analysis associates longevity to reduced growth, reprogrammed metabolism and cell wall remodeling. m:Explorer (http://biit.cs.ut.ee/mexplorer/) is instrumental in interrogating eukaryotic regulatory systems using heterogeneous data.
Eukaryotic transcriptional regulation is a core cellular process that governs the expression of genes. Understanding gene expression is crucial in explaining complex biological processes including development, disease and cancer. Transcription factors (TF) are key proteins that activate or repress transcription by binding sequence-specifically to DNA in promoter regions of target genes. Mapping such regulatory networks and TF functions is therefore an important goal of current biomedical research. In complex vertebrate organisms like human, this task is hindered by enormous genomic space, numerous cell types, and distinct experimental procedures with data that is often unsuitable for direct comparison. The relatively simple unicellular model organism budding yeast (S. cerevisiae) serves as a platform for regulatory genomics. Multiple types of global-scale data of yeast gene regulation are available to date, including microarrays with TF deletion (ΔTF) strains [1, 2], predictions of TF binding sites (TFBS) [3–5], and measurements of chromatin state such as nucleosome positioning . These data appear to be complete, however the agreement between transcript expression and TF binding events remains modest [2, 7]. While part of this controversy can be attributed to experimental and statistical noise, we may still lack significant details regarding the biological relationships among such heterogeneous information. Consequently high-throughput data constitute less reliable evidence and much functional knowledge is extracted from careful and expensive focused studies. Most TFs and their exact roles in cellular processes remain poorly understood. Therefore biologically meaningful computational analysis is an important challenge in deciphering cellular regulatory networks.
Computational prediction of TF function from gene expression and DNA binding data is an active area of research. Numerous algorithms have been published elsewhere, albeit few have been validated experimentally. Earliest approaches focused on a specific class of data and used alternative types of evidence for computational validation. For instance, microarray clustering followed by DNA motif discovery in gene promoters helped establish the genome-scale link between mRNA expression profiles and TF binding [8, 9]. Similarly, analysis of cell cycle expression patterns of TF-bound genes led to recovery of cell cycle TFs . More recent methods use statistical modeling to integrate multiple types of evidence. For example, ARACNE extracts transcriptional networks from numeric microarray data using mutual information , and MARINA is a down-stream method that identifies master regulators of these networks through association tests with TF binding target genes . The SAMBA biclustering algorithm studies matrices of regulators and target genes, and highlights regulatory relationships between genes and TFs that co-occur in clusters . The linear regression method REDUCE integrates numeric microarray data, DNA sequence and TF affinity matrices by modeling the linear relationship between gene expression levels and TF-DNA interactions . The GeneClass algorithm additionally integrates information about gene function, as it constructs decision trees of discrete microarray profiles and TF binding sites to select predictors of process-specific genes . While this method provides direct modeling of gene function, TFs and gene expression data are studied as independent predictors. Notably, none of the above methods take advantage of recent ΔTF microarrays that reveal regulator target genes [1, 2]. Nested effects models are designed to extract regulatory networks from perturbation data , although integration of TFBS and gene annotations is not supported. Nucleosome positioning measurements also remain unexplored in all above approaches. In summary, additional computational efforts are required for meaningful integration of versatile biological data.
Here we propose a method m:Explorer that uses multinomial logistic regression models to predict process-specific transcription factors. We aim to provide the following improvements in comparison to earlier methods. First, our method allows simultaneous analysis of four classes of data: (i) gene expression data, including perturbation screens, (ii) TF binding sites, (iii) chromatin state in gene promoters, and (iv) functional gene classification. The model is based on the assumption that TF target genes from perturbation screens and TF binding assays are equally informative about TF process specificity. Second, we reduce noise by including only high-confidence regulatory relationships, and do not assume linear relationships between regulators and target genes. Third, we integrate detailed information to better reflect underlying biology: multiple subprocesses may be studied in a single model, and chromatin state data are incorporated into TF binding site analysis. TF target genes with simultaneous evidence from gene expression and TFBS data are highlighted separately. Fourth, our analysis is robust to highly redundant biological networks, as statistical independence is not required. We use univariate models to study all TFs independently and avoid over-fitting that is characteristic to many model-based approaches. This is statistically valid under the assumption that a complex model may be understood by examining its components.
To test our method, we compiled a comprehensive dataset covering most TFs of the budding yeast. We benchmarked m:Explorer in a well-studied biological system and establish its improved performance in comparison to several similar methods. Then we used the tool to discover regulators of quiescence (G0, stationary phase), a cellular resting state that serves as a model of chronological ageing. Experimental validations of our predictions revealed nine TFs with significant impact on G0 viability. Besides demonstrating the applicability of our computational method, these findings are of great potential interest to yeast biologists and researchers of G0-related processes like ageing, development and cancer.
m:Explorer - multinomial logistic regression for inferring process-specific gene regulation
The first stage of our analysis involves data preprocessing and discretization in which high-confidence TF target genes are identified from multiple sources (Figure 1A). We assumed that genes responding to TF perturbation are likely targets of the regulator. We previously analyzed a large collection of ΔTF microarrays, extracted genes with significant up or down-regulation (moderated t-test, FDR p ≤ 0.05), and assigned these to perturbed regulators (Step 1, methods described in ). We also followed the assumption that TF binding in promoters is likely to indicate regulation of downstream genes, and binding sites in low nucleosome occupancy regions are more likely targets of TFs. We collected TF-DNA interactions from multiple datasets and classified genes as TF-bound if at least one dataset showed significant binding in 600 bp promoters (Step 2). We further categorized our TFBS collection into nucleosome-depleted TFBS (onesided t-test, FDR p ≤ 0.05) and sites with no nucleosome depletion. Next we integrated TF target genes into a genome-wide matrix, by assigning non-related genes to a baseline class and creating extra classes for genes with multiple evidence (Steps 3, 5).
Besides regulatory targets of transcription factors, our method requires a list of process-specific genes for which potential regulators are predicted. These may originate from literature, additional microarray datasets, pathway databases or biomedical ontologies. Several non-overlapping lists of genes may be provided to integrate further information about sub-process specificity, sample treatment or differential expression. These genes are organized similarly to TF targets (Steps 4, 5).
where ν corresponds to degrees of freedom and reflects number of model parameters. To predict all regulators to a process of interest, we test all TFs independently, correct for multiple testing and find TFs with significant chi-square p-values (Benjamini-Yekutieli FDR, p ≤ 0.05).
In summary, m:Explorer uses the multinomial regression framework to associate process genes with TF regulatory targets from TFBS maps, gene expression patterns and nucleosome positioning data. Our method finds candidate TFs whose targets are especially informative of process genes, and thus may regulate their expression.
Yeast TF dataset with perturbation targets, DNA binding sites and nucleosome positioning
We used m:Explorer to study transcriptional regulation and TF function in yeast, as it has the widest collection of relevant genome-wide evidence. First we compiled a dataset of 285 regulators that contains carefully selected target genes for nearly all yeast TFs from microarrays, DNA-binding assays and nucleosome positioning measurements. Statistically significant target genes from regulator deletion experiments originate from our recent reanalysis  of an earlier study . High-confidence TFBS targets were assembled from earlier chromatin immunoprecipitation (ChIP) assays by Harbison et al. , in silico TFBS predictions [4, 17], and recent refinements with protein-binding microarrays by Zhu et al. . The data were further processed with in vivo nucleosome positioning measurements  to distinguish binding sites where lower nucleosome occupancy reflects open chromatin structure.
In conclusion, the yeast TF dataset is a useful resource for studying gene regulation.
High-confidence recovery of cell cycle regulators
First we tested m:Explorer in a well-defined biological context. Cell cycle is a thoroughly described regulatory system with four consecutive phases: gap-1 (G1), synthesis (S), gap-2 (G2) and mitosis (M). Some of the earliest microarray experiments identified cell cycle-regulated yeast genes [18, 19], and a computational analysis organized these into phase-specific groups . Several focused studies have investigated the roles of individual cell cycle TFs [21–25], and a genome-wide experiment outlined the underlying regulatory network in its interconnected, circular nature . Altogether, the core cell cycle network comprises nine transcriptional regulators (Swi4, Swi6, Mbp1, Ndd1, Fkh1, Fkh2, Swi5, Ace2, Mcm1, Additional file 1, Table s1).
Our method reveals additional details about cell cycle regulation. First, as we model all cell cycle phases in one run, relative TF phase activities can be quantified through regression coefficients (Figure 3B). For instance Swi4, Swi6 and Mbp1 make up the G1-S specific TF complexes MBF and SBF , and m:Explorer correctly highlights the phases with the strongest signal of regulatory activity. Second, we can assess the relative contribution of different kinds of regulatory evidence, and show that combined TFBS and ΔTF evidence are most informative of cell cycle regulation (Figure 3C). Third, simultaneous analysis of multiple sub-processes in a single multinomial model is advantageous to separate logistic models for each related subprocess, since the latter approach is more prone to false positive predictions (Additional file 1, Figure s1). We performed m:Explorer analysis for four cell cycle phases and two checkpoints separately and recovered all cell cycle TFs found by the multinomial model, however also retrieved a large number (28) of additional false positive TFs not associated to cell cycle. Despite the above, analysis of sub-processes showed that m:Explorer is applicable to relatively small gene lists, for instance Mcm1 and Yox1 are correctly recovered as regulators of M-phase through only 55 informative genes.
Summary of comparison with similar methods
Univariate multinomial regression
Multivariate multinomial regression
Kolmogorov Smirnov test
Fisher's exact test
Univariate linear regression
Multivariate linear regression
In conclusion, the cell cycle analysis showed that our approach successfully recovers a well-characterized regulatory system from multiple lines of high-throughput data. m:Explorer greatly outperformed several similar methods and showed robustness to incomplete data.
Computational prediction of TFs for quiescence entry and maintenance
Next, we applied m:Explorer in a less familiar biological context to create experimentally verifiable hypotheses about TF function. We focused on the transcriptional mechanisms that govern cell quiescence (G0, reviewed by Gray  and Kaeberlein ). G0 is a cellular resting state with no proliferation, silenced genomes, reduced metabolism and translation, and greater stress resistance. Studying G0 has proven difficult and related regulatory programs remain elusive.
Here we studied two public microarray datasets and executed m:Explorer in two independent rounds. First, we retrieved 207 diauxic shift genes in three distinct subgroups of early, transient and late expression from the dataset by Radonjic et al. . Second, we used 594 genes and 676 genes characteristic of quiescent and non-quiescent cells from the study by Aragon et al.  (Figure 4B). We identified 29 and 82 statistically significant candidate TFs in the two runs, log-transformed the scores and produced a final list of 97 G0 regulators (Figure 4C). A large number of regulators is expected, as G0 entry is thought to comprise large-scale cellular reprogramming . Several top-ranking TFs have high scores in both m:Explorer predictions. This ranking is not an artifact of the overlap between diauxic shift and quiescence genes. Although the two lists comprise a considerable number of common genes (n = 62, p = 0.0005, Fisher's exact test), these were not sufficient for predicting a similar collection of G0 TFs, as m:Explorer analysis with the 62 genes only provided in a single significant TF (Mga2, p = 0.0005, LR test from m:Explorer).
In summary, the result of this analysis is an inclusive, prioritized list of candidate G0 TFs that serves as a resource for hypothesis generation and experimental testing.
Experimental validation reveals super-wildtype and essential G 0 TFs
All tested strains showed significant deviances from background viability at different stages of the quiescence time-course (Figure 5A). The deletion strains of Bas1, Sds3, cst6, Mga2, and Spt10 show consistently greater viability in G0, indicating that their normal presence in wildtype cells suppresses viability and hastens cell ageing (Figure 5C). We refer to these knockout phenotypes as super-wildtypes (WT+). In particular, Δbas1 strains are on average 1.7-4.5 times more viable than wildtype in weeks 3-6 of quiescence (all FDR p ≤ 10−4, LR test from error model). The transcription factor Bas1 is involved in the regulation of amino acid and nucleic acid metabolic pathways , and cst6 is related to chromosome stability and non-optimal carbon source regulation [38, 39]. Spt10 and Sds3 are chromatin modifiers involved in genome silencing [40, 41], and Mga2 regulates fatty acid metabolism, transcriptional silencing and response to low oxygen [42–44]. Deletion of Sds3 of the Sin3-Rpd3 histone deacetylase complex has been associated to increased chronological cell ageing .
The deletion strains Δtup1, Δswi3, Δhaa1 are significantly less viable than wildtype in quiescence (Figure 5A). In particular, Δtup1 and Δswi3 strains become inviable in later stages of G0 (viability ≤ 0.005) and can be considered essential for survival in this cell state (Figure 5D). Two further strains Δspt20 and Δsnf2 are less viable in early quiescence, while Δsin3 shows later deviations. With the exceptions of Sin3 and Haa1, corresponding null mutants are previously known for decreased or absent respiratory growth. Tup1 is a general inhibitor of transcription that establishes repressive chromatin structure . Other factors are also involved in regulation of chromatin, transcription and genome stability, such as Swi3 and Snf2 of the SWI-SNF complex , Sin3 of Sin3-Rpd3 complex  and Spt20 of the SAGA complex . While the factors have not been specifically described in the context of quiescence, disruption of their global functions is likely to affect this cellular state. Besides the above, the reduced G0 viability of Δ haa1 potentially relates to its role in regulating cell wall proteins .
Before entering quiescence, most tested TF strains have similar viability to wildtype strains, suggesting that their function in regulating viability is specific to G0 (Figure 5B). During exponential growth at seven hours after inoculation, only three strains including the positive control Δ ard1 are significantly less viable. Ard1 encodes an N-terminal acetyltransferase subunit that guides genome silencing, and Δard1 fails to enter G0 as observed previously . In contrast, the other positive control Δmip1 is as viable as wildtype in exponential phase, and more viable in post-diauxic phase. Mip1 encodes a mitochondrial DNA polymerase subunit required for cell respiration , and Δmip1 loses viability in a similar manner to Δ tup1 (Figure 5E). Curiously, Δspt10 is less viable in exponential growth phase and early quiescence, while its viability exceeds wildtype after week three of our time-course. The negative control strains Δgal3 and Δpdr3 expectedly show no major deviations from wildtype viability (Figure 5F). The TFs are related to alternative carbon metabolism and drug resistance, respectively [53, 54], and show non-significant scores in m:Explorer predictions of G0 TFs. Finally, our glycerol growth assays confirm the respiratory properties of tested strains (Additional file 1, Table s3) and mostly agree with previous studies [55, 56]. However, in contrast to those reports, our data indicate that Δcst6 is viable on glycerol and indeed displays increased G0 viability.
According to our knowledge, most of our predicted TFs are not recognized as quiescence regulators. However previous functional evidence refers to processes important in quiescence, and hence lends confidence to our experimental observations. Besides uncovering novel regulators of viability in G0, our experiments show that m:Explorer provides biologically meaningful prediction of regulator function.
Functional enrichment analysis explains roles of G 0 TFs
To gain insight into G0 gene regulation of validated TFs, we performed a functional enrichment analysis of their G0 target genes. We focused on quiescence genes defined by Aragon et al.  and identified the subset of genes that were bound by at least one WT+ TF or showed differential gene expression in at least one WT+ ΔTF microarray . Target genes were then scored by product of differential expression p-values across all WT+ ΔTF microarrays and ranked such that genes with most dramatic transcriptional changes were prioritized. The target gene list for viability-deficient TF strains was complied in a similar fashion. We expect that ΔTF differential expression is informative of regulatory relationships in quiescence. The strains underlying microarray profiling are genetically identical to the strains in our G0 experiments, although the former assays were performed with exponentially growing cells. Intersection of known quiescence genes with target genes of validated G0 TFs, and subsequent prioritization according to differential expression, is therefore likely to highlight high-confidence TF targets and functional relationships. To investigate this in detail, we then used the ordered gene list analysis of g:Profiler  to study the functional importance of significance-ranked target genes of WT+ and viability-deficient TFs.
Taken together, the above results associate to known mechanisms of quiescence and provide clues of the regulatory programs of predicted G0 TFs. Inhibition of growth through down-regulation of ribosome genes has been linked to increased replicative lifespan . Efficient cell wall remodeling and response to increased oxidative stress are essential prerequisites of quiescence entry and survival . Expectedly, increased viability appears to correlate with reduced metabolism, as related genes show opposite expression patterns in corresponding strains. Further discussion on G0 TFs and related pathways can be found below.
Function of G 0 regulators
It is tempting to speculate about the role of identified quiescence TFs in modulating quiescence signalling, as links between the factors and global G0-related pathways are apparent in our dataset. Our findings of WT+ regulators are especially intriguing, since their normal presence in wildtype cells reduces viability in quiescence and causes increased chronological ageing. From the perspective of evolutionary maintenance, WT+ regulators should engage in significant cellular functions that compensate for such negative properties.
As an example of G0 regulation, protein kinase A (PKA) mediates nutritional signals to the cell and is known as an inhibitor of quiescence . Its primary regulatory subunit Bcy1 acts as an inhibitor of the pathway, and mutations in Bcy1 cause viability loss and death in G0 [59, 60]. This double negative regulatory mechanism provides a potential explanation to observed viability phenotypes. In our TF dataset, Δmga2 has significantly higher levels of Bcy1, potentially allowing more starving cells to pass into quiescence. The G0-essential Tup1 and Swi3 knockout strains have depleted levels of Bcy1 and as a possible consequence, we observe reduction and loss of viability. As another example, protein kinase C (PKC) guides cell wall remodeling in response to starvation and its activity is required for G0 entry . The cell wall biosynthesis enzyme Gsc2 is a downstream target of PKC  and part of the gene expression signature of quiescent cells . In ΔTF microarrays, Δmga2 and Δcst6 strains have elevated levels of Gsc2, while Δswi3 and Δtup1 show inhibition of PKC upstream of Gsc2 (Additional file 1, Figure s5). Other genes with known function in G0 appear to be regulated by WT+ and viability-deficient TFs. Notably, the conserved superoxide dismutase (SOD) genes are responsible for neutralizing oxidative damage of mitochondrial respiration. In yeast, SOD genes are required for G0 survival and extend chronological lifespan when over-expressed [63, 64]. Induced levels of Sod2 expression in Δcst6 may explain our observations of increased G0 viability.
Several confirmed G0 TFs are also associated to mammalian gene regulation.Cst6 carries the DNA-binding domain of CREB, an extensively studied TF that regulates a variety of processes, including cell survival and proliferation, cellular metabolism, and synaptic plasticity of long-term memory . Bas1 is homologous to the MYB TF that regulates stem and progenitor cells and appears as an oncogene in multiple tumour types . Chromatin modifier complexes Swi/Snf, Sin3/Rpd3 and SAGA are also broadly conserved, for instance Swi3 homolog SMARCC1 is involved in versatile functions, including neural stem cell renewal and differentiation . As the yeast quiescence model associates to hallmark cancer properties of cell cycle control, proliferation and differentiation, further analysis of our Findings may reveal intriguing links to cancer biology.
Applicability and validity of m:Explorer
Here we present the robust computational method m:Explorer for predicting functions of gene regulators from high-throughput data. We applied a model that probabilistically accounts for multiple types of regulatory signals and functional gene annotations. To take advantage of abundant genome-wide data and powerful experimental approaches, we present a case study for predicting transcription factors (TF) in the unicellular budding yeast. However, our method is not restricted to yeast and even not to these classes of data and regulators, being easily scalable to more complex regulatory systems of vertebrate organisms. Our method is also applicable to data such as protein-protein and genetic interactions that are categorical in nature. As shown here, m:Explorer is particularly useful in investigating sparse, high-confidence sets of data that may be controversial and not entirely comparable. For instance, we envisage large-scale characterization of human pathways in the context of heterogeneous tumours, utilizing sequence mutations, gene expression and chromatin modification data that are collected in cancer genomics projects.
In our model benchmarks, we demonstrate the advantage of univariate multinomial models in m:Explorer over similar multivariate models (AUC = 0.83 vs AUC = 0.67 for recovering cell cycle TFs). Briefly, the former models treat each TF independently in process gene classification, while the latter models include a non redundant collection of TFs as predictors. However, TF redundancy is an inherent property of robust biological networks that have evolved through gene and genome duplication . In our case, the core cell cycle system involves three pairs of homologous TFs (Swi4 and Swi6; Fkh1 and Fkh2; Ace2 and Swi5) that have strikingly similar TFBS and expression patterns. Due to redundancy, such TFs are not treated as significant predictors in the multivariate setting. This is evident in our simulations: none of the tested multivariate models included both TFs of homologous pairs as significant predictors.
This analysis provides multiple lines of evidence to establish m:Explorer among other methods with similar goals. First, we carried out a highly detailed reconstruction of the known cell cycle regulatory system and proved the validity of our approach through existing knowledge. Second, we repeated the same analysis using eight alternative computational methods and random samples of input data, and provided quantitative proof to the robustness and better performance of our method. Third, we predicted regulators to the enigmatic cellular state of quiescence and validated our top-ranking candidate TFs in follow-up experiments. Nine of twelve tested TFs were confirmed to have consistent and significant G0 viability deviations in gene knockout screens, while the remaining three factors showed differences in subsections of our time-course. Thus we proved a high success rate given our relatively simple experimental assays. Besides demonstrating the biological validity of our method, our findings reveal novel, previously unrecognized regulators of quiescence.
m:Explorer web server and data availability
m:Explorer is available as an R package on our web site  and elsewhere. The yeast TF dataset may prove to be a useful resource for the community and is also provided. We have established a web server at , allowing online prediction of regulator function using the yeast TF dataset.
m:Explorer is a generally applicable method for inferring transcription factor function from heterogeneous high-throughput datasets. Our approach outperforms similar state-of-the art tools in recovering regulatory relationships in a well-studied eukaryotic system. Furthermore, the algorithm helps explore uncharacterized regulatory networks and propose valuable hypotheses for detailed assays. Our case study of quiescence G0and subsequent experimental validations revealed nine novel regulators that enhance or reduce cellular longevity, providing insights to investigators of this cryptic cellular state. In conclusion, our computational and experimental analyses provide strong support to the validity and usefulness of m:Explorer.
Materials and methods
The yeast transcription factor dataset of 6253 genes and 285 transcription factors was compiled from gene expression, TF binding and nucleosome positioning data. Perturbation microarrays for 269 regulators were originally produced by Hu et al. , while our recently reanalyzed dataset  was used here for discretized, high-confidence values of up- and down-regulation (moderated t-test, FDR p ≤ 0.05). Further details on microarray preprocessing are available in the related publication . TF binding site data for 178 TFs were compiled from multiple datasets of ChIP-chip , protein-binding microarrays  and computational predictions [4, 17], using custom filtering and significance cutoffs proposed by the authors. Each promoter of 600 bp was considered to be bound by a TF if at least one binding site occurred in the dataset, and the TFBS was considered nucleosome-depleted (NDTFBS) if nucleosome occupancy  at the site was considerably below normalized genome-wide average (t-test, FDR p ≤ 0.05). Finally, gene expression and TF binding targets for each regulator were integrated and split into eight classes (up, down, TFBS, NDTFBS, up+TFBS, down+TFBS, up+NDTFBS, down+NDTFBS). All other genes except TF targets were assigned to the baseline class (not regulated).
Process-specific gene lists originate from previous high-throughput gene expression experiments. 600 cell cycle specific genes were retrieved from the tiling array experiment by Granovskaia et al.  and split into six sublists (G1, S, G2, G2/M, M, M/G1) according to authors' instructions. Three classes of diauxic shift genes (early, transient and late expression) originate from the G0 time series , and genes specific to quiescent and non-quiescent cell cultures were first mapped in the analysis by Aragon et al. .
m:Explorer is based on univariate multinomial regression and implements the functionality of the R NNET package  for model fitting. We use a list of process-specific genes as categorical model response, and TF target genes as predictors. Briefly, m:Explorer compares two models: the null intercept-only model classifies process gene through their frequency, and the alternative univariate model additionally incorporates TF regulatory targets as predictors. We apply the log likelihood ratio test with null and alternative models to decide if TF target genes are significantly informative of process-related genes. Detailed description of the model is available in Additional file 1.
Yeast cell cycle TFs were predicted from a single structured gene list and directly ranked according to log p-values from m:Explorer. G0 TFs were predicted in two independent m:Explorer runs using genes from two datasets. TF p-values from LR tests were log-transformed, scaled to unit range and summed across the two runs to create unbiased composite scores for final ranking. Unit-scaled positive regression coefficients were used to assess the relative phase specificity of cell cycle TFs, since these indicate over-represented regulatory targets in contrast to baseline genes. Relative contribution of regulatory evidence was computed in a similar way.
Linear regression was used to assess the significance of mutant strain viability deviations from control and wildtype strains. With viability as model response v, three types of variance were included as model predictors for assessing each mutant/time-point combination across all related replicas, as the alternative model H1 : v ~ i + c + b + m. The above reflect global variance i, variance of negative controls c, variance between two batches of independent time-courses b, and additional variance of the tested strain m. Significance of viability deviation was assessed with a LR test, similarly to the m:Explorer algorithm. Specifically, the null model comprised only global variance, negative control variance and batch variance as H0 : v ~ i + c + b, and null and alternative models were compared using the chi-square distribution. Resulting p-values were corrected for multiple testing with FDR.
where g denotes the number of genes in a particular set, C indicates cell cycle genes, T indicates TF targets, c shows genes unrelated to cell cycle, t shows genes not regulated by the particular TF, and n = g CT +g Ct +g cT +g ct reflects the number of all yeast genes. As Fisher's test does not support large contingency tables of multi-level variables, different types of TF regulatory targets were treated as the first category and non-regulated genes were assigned to second category, and cell cycle phase-specific genes were similarly merged into a bivariate discrete variable. A similar analysis was carried out to compare the overlap between diauxic shift genes and quiescence genes, using the set of all yeast genes as statistical background.
Gene Ontology (GO) and pathway enrichment analysis for G0 TFs was carried out with with g:Profiler software . We defined two ranked gene lists: G0 genes  that were differentially expressed in WT+ TF knockout strains (Mga2, Cst6, Sds3, Spt10, Bas1), and G0 genes that were differentially expressed in viability-deficient TF strains (Swi3, Sin3, Snf2, Spt20, Tup1, Haa1), according to TF knockout microarrays . The gene lists were ordered according to statistical significance in TF knockout data , computed as products of p-values across WT+ and RD strains for every gene. We used the ordered enrichment analysis of g:Profiler to find GO functions and pathways in ranked gene lists and applied statistical filtering to find significant enrichments (FDR p ≤ 0.05).
given that there are N genes in total and K of which are part of the functional category. As ordered enrichment analysis assumes that genes with stronger signals are ranked first, it consequently tests different subsets of the top list and returns the portion of top genes with the strongest p-value for a particular functional category . Resulting G0 functional categories were grouped into three classes: enriched G0 categories associating to WT+ TF targets, categories of viability-deficient TF targets, and categories with statistical enrichment in both groups of targets. Enrichment p-values were corrected for multiple testing with the FDR procedure. To rank the third class of common functional categories, we multiplied corresponding p-values of WT+ target genes and viability-deficient TF target genes. After functional enrichment analysis, redundant categories whose genes formed a subset of some other category were removed. To quantify each GO category and function, we also counted up-regulated and down-regulated G0 genes across all related TF strains.
Regulator knockout strains were selected as 12 top-ranking candidates from m:Explorer results. S. cerevisiae deletion strains originate from the EUROSCARF deletion collection in the BY4741 strain (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0). Liquid cultures were grown in triplicate at 30°C with aeration in YPD (1% yeast extract, 2% peptone, 2% glucose) for 28 days and subsequently shifted to room temperature without aeration. Viability measurements of the six-week time-course were taken in eight time-points: 7h after colony initiation, 48h after colony initiation, followed by six weekly measurements on days 7, 14, 21, 28, 35 and 42. Two independent batches involved distinct sets of tested strains, while wildtypes and controls were covered in both batches. A shorter, independent time-course covered the first three days of growth and involved viability measurements at 7h, 11h, 24h, 48h, and 72h. Cell density was measured at 600 nm. Colony forming units (CFU/ml) were determined by plating cells on YPD agar and counting colonies after three days of growth at 30°C. Culture viability was determined by dividing CFU/ml with total cell number per milliliter in corresponding culture (OD600 units ×107). Growth on glycerol was determined by streaking strains onto YPG plates (1% yeast extract, 2% peptone, 3% glycerol, 2% agar). Glucose concentration was determined by measuring NADPH production in hexokinase and glucose-6-phosphate dehydrogenase coupled reactions provided by Roche.
We are grateful to Gary Bader, Richard Bourgon, John Marioni, Gabriella Rustici, and Annabel Todd for their valuable advice, and to Tambet Arak for his work on the m:Explorer website. JR would like to acknowledge financial support from IKTDK, EXCS, ENFIN, EITSA, Marie Curie Biostar, the Ustus Agur Foundation, and the Artur Lind Foundation. Computational resources were provided by the University of Tartu HPC Centre.
- Hu Z, Killion PJ, Iyer VR: Genetic reconstruction of a functional transcriptional regulatory network. Nat Genet. 2007, 39: 683-687. 10.1038/ng2012.PubMedView ArticleGoogle Scholar
- Reimand J, Vaquerizas JM, Todd AE, Vilo J, Luscombe NM: Comprehensive reanalysis of transcription factor knockout expression data in Saccharomyces cerevisiae reveals many new targets. Nucleic Acids Res. 2010, 38: 4768-4777. 10.1093/nar/gkq232.PubMedPubMed CentralView ArticleGoogle Scholar
- Harbison CT, Gordon DB, Lee TI, Rinaldi NJ, Macisaac KD, Danford TW, Hannett NM, Tagne JB, Reynolds DB, Yoo J, Jennings EG, Zeitlinger J, Pokholok DK, Kellis M, Rolfe PA, Takusagawa KT, Lander ES, Gifford DK, Fraenkel E, Young RA: Transcriptional regulatory code of a eukaryotic genome. Nature. 2004, 431: 99-104. 10.1038/nature02800.PubMedPubMed CentralView ArticleGoogle Scholar
- MacIsaac KD, Wang T, Gordon DB, Gifford DK, Stormo GD, Fraenkel E: An improved map of conserved regulatory sites for Saccharomyces cerevisiae. BMC Bioinformatics. 2006, 7: 113-10.1186/1471-2105-7-113.PubMedPubMed CentralView ArticleGoogle Scholar
- Zhu C, Byers KJ, McCord RP, Shi Z, Berger MF, Newburger DE, Saulrieta K, Smith Z, Shah MV, Radhakrishnan M, Philippakis AA, Hu Y, De Masi F, Pacek M, Rolfs A, Murthy T, Labaer J, Bulyk ML: High-resolution DNA-binding specificity analysis of yeast transcription factors. Genome Res. 2009, 19: 556-566. 10.1101/gr.090233.108.PubMedPubMed CentralView ArticleGoogle Scholar
- Kaplan N, Moore IK, Fondufe-Mittendorf Y, Gossett AJ, Tillo D, Field Y, LeProust EM, Hughes TR, Lieb JD, Widom J, Segal E: The DNA-encoded nucleosome organization of a eukaryotic genome. Nature. 2009, 458: 362-366. 10.1038/nature07667.PubMedPubMed CentralView ArticleGoogle Scholar
- Balaji S, Iyer LM, Babu MM, Aravind L: Comparison of transcription regulatory interactions inferred from high-throughput methods: what do they reveal?. Trends Genet. 2008, 24: 319-323. 10.1016/j.tig.2008.04.006.PubMedPubMed CentralView ArticleGoogle Scholar
- Eisen MB, Spellman PT, Brown PO, Botstein D: Cluster analysis and display of genome-wide expression patterns. Proc Natl Acad Sci USA. 1998, 95: 14863-14868. 10.1073/pnas.95.25.14863.PubMedPubMed CentralView ArticleGoogle Scholar
- Brazma A, Jonassen I, Vilo J, Ukkonen E: Predicting gene regulatory elements in silico on a genomic scale. Genome Res. 1998, 8: 1202-1215.PubMedPubMed CentralGoogle Scholar
- Tsai HK, Lu HH, Li WH: Statistical methods for identifying yeast cell cycle transcription factors. Proc Natl Acad Sci USA. 2005, 102: 13532-13537. 10.1073/pnas.0505874102.PubMedPubMed CentralView ArticleGoogle Scholar
- Margolin AA, Nemenman I, Basso K, Wiggins C, Stolovitzky G, Dalla Favera R, Califano A: ARACNE: an algorithm for the reconstruction of gene regulatory networks in a mammalian cellular context. BMC Bioinformatics. 2006, 7 (Suppl 1): S7-10.1186/1471-2105-7-S1-S7.PubMedPubMed CentralView ArticleGoogle Scholar
- Lefebvre C, Rajbhandari P, Alvarez MJ, Bandaru P, Lim WK, Sato M, Wang K, Sumazin P, Kustagi M, Bisikirska BC, Basso K, Beltrao P, Krogan N, Gautier J, Dalla-Favera R, Califano A: A human B-cell interactome identifies MYB and FOXM1 as master regulators of proliferation in germinal centers. Mol Syst Biol. 2010, 6: 377-PubMedPubMed CentralView ArticleGoogle Scholar
- Tanay A, Sharan R, Kupiec M, Shamir R: Revealing modularity and organization in the yeast molecular network by integrated analysis of highly heterogeneous genomewide data. Proc Natl Acad Sci USA. 2004, 101 (9): 2981-2986. 10.1073/pnas.0308661100.PubMedPubMed CentralView ArticleGoogle Scholar
- Bussemaker HJ, Li H, Siggia ED: Regulatory element detection using correlation with expression. Nat Genet. 2001, 27: 167-171. 10.1038/84792.PubMedView ArticleGoogle Scholar
- Middendorf M, Kundaje A, Wiggins C, Freund Y, Leslie C: Predicting genetic regulatory response using classification. Bioinformatics. 2004, 20 (Suppl 1): i232-240. 10.1093/bioinformatics/bth923.PubMedView ArticleGoogle Scholar
- Markowetz F, Bloch J, Spang R: Non-transcriptional pathway features reconstructed from secondary effects of RNA interference. Bioinformatics. 2005, 21: 4026-4032. 10.1093/bioinformatics/bti662.PubMedView ArticleGoogle Scholar
- Erb I, van Nimwegen E: Statistical features of yeast's transcriptional regulatory code. IEEE Proceedings ICCSB. 2006Google Scholar
- Cho RJ, Campbell MJ, Winzeler EA, Steinmetz L, Conway A, Wodicka L, Wolfsberg TG, Gabrielian AE, Landsman D, Lockhart DJ, Davis RW: A genome-wide transcriptional analysis of the mitotic cell cycle. Mol Cell. 1998, 2: 65-73. 10.1016/S1097-2765(00)80114-8.PubMedView ArticleGoogle Scholar
- Spellman PT, Sherlock G, Zhang MQ, Iyer VR, Anders K, Eisen MB, Brown PO, Botstein D, Futcher B: Comprehensive identification of cell cycle-regulated genes of the yeast Saccharomyces cerevisiae by microarray hybridization. Mol Biol Cell. 1998, 9: 3273-3297.PubMedPubMed CentralView ArticleGoogle Scholar
- de Lichtenberg U, Jensen LJ, Fausbøll A, Jensen TS, Bork P, Brunak S: Comparison of computational methods for the identification of cell cycle-regulated genes. Bioinformatics. 2005, 21: 1164-1171. 10.1093/bioinformatics/bti093.PubMedView ArticleGoogle Scholar
- Koch C, Moll T, Neuberg M, Ahorn H, Nasmyth K: A role for the transcription factors Mbp1 and Swi4 in progression from G1 to S phase. Science. 1993, 261: 1551-1557. 10.1126/science.8372350.PubMedView ArticleGoogle Scholar
- Kumar R, Reynolds DM, Shevchenko A, Shevchenko A, Goldstone SD, Dalton S: Forkhead transcription factors, Fkh1p and Fkh2p, collaborate with Mcm1p to control transcription required for M-phase. Curr Biol. 2000, 10: 896-906. 10.1016/S0960-9822(00)00618-7.PubMedView ArticleGoogle Scholar
- McBride HJ, Yu Y, Stillman DJ: Distinct regions of the Swi5 and Ace2 transcription factors are required for specific gene activation. J Biol Chem. 1999, 274: 21029-21036. 10.1074/jbc.274.30.21029.PubMedView ArticleGoogle Scholar
- Loy CJ, Lydall D, Surana U: NDD1, a high-dosage suppressor of cdc28-1N, is essential for expression of a subset of late-S-phase-specific genes in Saccharomyces cerevisiae. Mol Cell Biol. 1999, 19: 3312-3327.PubMedPubMed CentralView ArticleGoogle Scholar
- Althoefer H, Schleiffer A, Wassmann K, Nordheim A, Ammerer G: Mcm1 is required to coordinate G2-specific transcription in Saccharomyces cerevisiae. Mol Cell Biol. 1995, 15: 5917-5928.PubMedPubMed CentralView ArticleGoogle Scholar
- Simon I, Barnett J, Hannett N, Harbison CT, Rinaldi NJ, Volkert TL, Wyrick JJ, Zeitlinger J, Gifford DK, Jaakkola TS, Young RA: Serial regulation of transcriptional regulators in the yeast cell cycle. Cell. 2001, 106: 697-708. 10.1016/S0092-8674(01)00494-9.PubMedView ArticleGoogle Scholar
- Granovskaia MV, Jensen LJ, Ritchie ME, Toedling J, Ning Y, Bork P, Huber W, Steinmetz LM: High-resolution transcription atlas of the mitotic cell cycle in budding yeast. Genome Biol. 2010, 11: R24-10.1186/gb-2010-11-3-r24.PubMedPubMed CentralView ArticleGoogle Scholar
- Costanzo M, Schub O, Andrews B: G1 transcription factors are differentially regulated in Saccharomyces cerevisiae by the Swi6-binding protein Stb1. Mol Cell Biol. 2003, 23: 5064-5077. 10.1128/MCB.23.14.5064-5077.2003.PubMedPubMed CentralView ArticleGoogle Scholar
- Pramila T, Miles S, GuhaThakurta D, Jemiolo D, Breeden LL: Conserved homeodomain proteins interact with MADS box protein Mcm1 to restrict ECB-dependent transcription to the M/G1 phase of the cell cycle. Genes Dev. 2002, 16: 3034-3045. 10.1101/gad.1034302.PubMedPubMed CentralView ArticleGoogle Scholar
- Oehlen LJ, McKinney JD, Cross FR: Ste12 and Mcm1 regulate cell cycle-dependent transcription of FAR1. Mol Cell Biol. 1996, 16: 2830-2837.PubMedPubMed CentralView ArticleGoogle Scholar
- Kaeberlein M, McVey M, Guarente L: The SIR2/3/4 complex and SIR2 alone promote longevity in Saccharomyces cerevisiae by two different mechanisms. Genes Dev. 1999, 13: 2570-2580. 10.1101/gad.13.19.2570.PubMedPubMed CentralView ArticleGoogle Scholar
- Gray JV, Petsko GA, Johnston GC, Ringe D, Singer RA, Werner-Washburne M: Sleeping beauty":quiescence in Saccharomyces cerevisiae. Microbiol Mol Biol Rev. 2004, 68: 187-206. 10.1128/MMBR.68.2.187-206.2004.PubMedPubMed CentralView ArticleGoogle Scholar
- Kaeberlein M, Burtner CR, Kennedy BK: Recent developments in yeast aging. PLoS Genet. 2007, 3: e84-10.1371/journal.pgen.0030084.PubMedPubMed CentralView ArticleGoogle Scholar
- Allen C, Buttner S, Aragon AD, Thomas JA, Meirelles O, Jaetao JE, Benn D, Ruby SW, Veenhuis M, Madeo F, Werner-Washburne M: Isolation of quiescent and nonquiescent cells from yeast stationary-phase cultures. J Cell Biol. 2006, 174: 89-100. 10.1083/jcb.200604072.PubMedPubMed CentralView ArticleGoogle Scholar
- Radonjic M, Andrau JC, Lijnzaad P, Kemmeren P, Kockelkorn TT, van Leenen D, van Berkum NL, Holstege FC: Genome-wide analyses reveal RNA polymerase II located upstream of genes poised for rapid response upon S. cerevisiae stationary phase exit. Mol Cell. 2005, 18: 171-183. 10.1016/j.molcel.2005.03.010.PubMedView ArticleGoogle Scholar
- Aragon AD, Rodriguez AL, Meirelles O, Roy S, Davidson GS, Tapia PH, Allen C, Joe R, Benn D, Werner-Washburne M: Characterization of differentiated quiescent and nonquiescent cells in yeast stationary-phase cultures. Mol Biol Cell. 2008, 19: 1271-1280.PubMedPubMed CentralView ArticleGoogle Scholar
- Denis V, Boucherie H, Monribot C, Daignan-Fornier B: Role of the myb-like protein bas1p in Saccharomyces cerevisiae: a proteome analysis. Mol Microbiol. 1998, 30: 557-566. 10.1046/j.1365-2958.1998.01087.x.PubMedView ArticleGoogle Scholar
- Ouspenski II, Elledge SJ, Brinkley BR: New yeast genes important for chromosome integrity and segregation identified by dosage effects on genome stability. Nucleic Acids Res. 1999, 27: 3001-3008. 10.1093/nar/27.15.3001.PubMedPubMed CentralView ArticleGoogle Scholar
- Garcia-Gimeno MA, Struhl K: Aca1 and Aca2, ATF/CREB activators in Saccharomyces cerevisiae, are important for carbon source utilization but not the response to stress. Mol Cell Biol. 2000, 20: 4340-4349. 10.1128/MCB.20.12.4340-4349.2000.PubMedPubMed CentralView ArticleGoogle Scholar
- riksson PR, Mendiratta G, McLaughlin NB, Wolfsberg TG, Marino-Ramirez L, Pompa TA, Jainerin M, Landsman D, Shen CH, Clark DJ: Global regulation by the yeast Spt10 protein is mediated through chromatin structure and the histone upstream activating sequence elements. Mol Cell Biol. 2005, 25: 9127-9137. 10.1128/MCB.25.20.9127-9137.2005.View ArticleGoogle Scholar
- Vannier D, Balderes D, Shore D: Evidence that the transcriptional regulators SIN3 and RPD3, and a novel gene (SDS3) with similar functions, are involved in transcriptional silencing in S. cerevisiae. Genetics. 1996, 144: 1343-1353.PubMedPubMed CentralGoogle Scholar
- Dula ML, Holmes SG: MGA2 and SPT23 are modifiers of transcriptional silencing in yeast. Genetics. 2000, 156: 933-941.PubMedPubMed CentralGoogle Scholar
- Chellappa R, Kandasamy P, Oh CS, Jiang Y, Vemula M, Martin CE: The membrane proteins, Spt23p and Mga2p, play distinct roles in the activation of Saccharomyces cerevisiae OLE1 gene expression. Fatty acid-mediated regulation of Mga2p activity is independent of its proteolytic processing into a soluble transcription activator. J Biol Chem. 2001, 276: 43548-43556. 10.1074/jbc.M107845200.PubMedView ArticleGoogle Scholar
- Jiang Y, Vasconcelles MJ, Wretzel S, Light A, Martin CE, Goldberg MA: MGA2 is involved in the low-oxygen response element-dependent hypoxic induction of genes in Saccharomyces cerevisiae. Mol Cell Biol. 2001, 21: 6161-6169. 10.1128/MCB.21.18.6161-6169.2001.PubMedPubMed CentralView ArticleGoogle Scholar
- Eisenberg T, Knauer H, Schauer A, Buttner S, Ruckenstuhl C, Carmona-Gutierrez D, Ring J, Schroeder S, Magnes C, Antonacci L, Fussi H, Deszcz L, Hartl R, Schraml E, Criollo A, Megalou E, Weiskopf D, Laun P, Heeren G, Breitenbach M, Grubeck-Loebenstein B, Herker E, Fahrenkrog B, Frohlich KU, Sinner F, Tavernarakis N, Minois N, Kroemer G, Madeo F: Induction of autophagy by spermidine promotes longevity. Nat Cell Biol. 2009, 11: 1305-1314. 10.1038/ncb1975.PubMedView ArticleGoogle Scholar
- Courey AJ, Jia S: Transcriptional repression: the long and the short of it. Genes Dev. 2001, 15: 2786-2796.PubMedGoogle Scholar
- Sudarsanam P, Winston F: The Swi/Snf family nucleosome-remodeling complexes and transcriptional control. Trends Genet. 2000, 16: 345-351. 10.1016/S0168-9525(00)02060-6.PubMedView ArticleGoogle Scholar
- Silverstein RA, Ekwall K: Sin3: a flexible regulator of global gene expression and genome stability. Curr Genet. 2005, 47: 1-17. 10.1007/s00294-004-0541-5.PubMedView ArticleGoogle Scholar
- Sterner DE, Grant PA, Roberts SM, Duggan LJ, Belotserkovskaya R, Pacella LA, Winston F, Workman JL, Berger SL: Functional organization of the yeast SAGA complex: distinct components involved in structural integrity, nucleosome acetylation, and TATA-binding protein interaction. Mol Cell Biol. 1999, 19: 86-98.PubMedPubMed CentralView ArticleGoogle Scholar
- Simoes T, Mira NP, Fernandes AR, Sa-Correia I: The SPI1 gene, encoding a glycosylphosphatidylinositol-anchored cell wall protein, plays a prominent role in the development of yeast resistance to lipophilic weak-acid food preservatives. Appl Environ Microbiol. 2006, 72: 7168-7175. 10.1128/AEM.01476-06.PubMedPubMed CentralView ArticleGoogle Scholar
- Mullen JR, Kayne PS, Moerschell RP, Tsunasawa S, Gribskov M, Colavito-Shepanski M, Grunstein M, Sherman F, Sternglanz R: Identification and characterization of genes and mutants for an N-terminal acetyltransferase from yeast. EMBO J. 1989, 8: 2067-2075.PubMedPubMed CentralGoogle Scholar
- Foury F: Cloning and sequencing of the nuclear gene MIP1 encoding the catalytic subunit of the yeast mitochondrial DNA polymerase. J Biol Chem. 1989, 264: 20552-20560.PubMedGoogle Scholar
- Torchia TE, Hopper JE: Genetic and molecular analysis of the GAL3 gene in the expression of the galactose/melibiose regulon of Saccharomyces cerevisiae. Genetics. 1986, 113: 229-246.PubMedPubMed CentralGoogle Scholar
- Delaveau T, Delahodde A, Carvajal E, Subik J, Jacq C: PDR3, a new yeast regulatory gene, is homologous to PDR1 and controls the multidrug resistance phenomenon. Mol Gen Genet. 1994, 244: 501-511. 10.1007/BF00583901.PubMedView ArticleGoogle Scholar
- Steinmetz LM, Scharfe C, Deutschbauer AM, Mokranjac D, Herman ZS, Jones T, Chu AM, Giaever G, Prokisch H, Oefner PJ, Davis RW: Systematic screen for human disease genes in yeast. Nat Genet. 2002, 31: 400-404.PubMedGoogle Scholar
- Dimmer KS, Fritz S, Fuchs F, Messerschmitt M, Weinbach N, Neupert W, Westermann B: Genetic basis of mitochondrial function and morphology in Saccharomyces cerevisiae. Mol Biol Cell. 2002, 13: 847-853. 10.1091/mbc.01-12-0588.PubMedPubMed CentralView ArticleGoogle Scholar
- Reimand J, Arak T, Vilo J: g:Profiler-a web server for functional interpretation of gene lists (2011 update). Nucleic Acids Res. 2011, 39: W307-315. 10.1093/nar/gkr378.PubMedPubMed CentralView ArticleGoogle Scholar
- Kaeberlein M, Powers RW, Steffen KK, Westman EA, Hu D, Dang N, Kerr EO, Kirkland KT, Fields S, Kennedy BK: Regulation of yeast replicative life span by TOR and Sch9 in response to nutrients. Science. 2005, 310: 1193-1196. 10.1126/science.1115535.PubMedView ArticleGoogle Scholar
- Santangelo GM: Glucose signaling in Saccharomyces cerevisiae. Microbiol Mol Biol Rev. 2006, 70: 253-282. 10.1128/MMBR.70.1.253-282.2006.PubMedPubMed CentralView ArticleGoogle Scholar
- Peck VM, Fuge EK, Padilla PA, Gomez MA, Werner-Washburne M: Yeast bcy1 mutants with stationary phase-specific defects. Curr Genet. 1997, 32: 83-92. 10.1007/s002940050251.PubMedView ArticleGoogle Scholar
- Krause SA, Gray JV: The protein kinase C pathway is required for viability in quiescence in Saccharomyces cerevisiae. Curr Biol. 2002, 12: 588-593. 10.1016/S0960-9822(02)00760-1.PubMedView ArticleGoogle Scholar
- Zhao C, Jung US, Garrett-Engele P, Roe T, Cyert MS, Levin DE: Temperature-induced expression of yeast FKS2 is under the dual control of protein kinase C and calcineurin. Mol Cell Biol. 1998, 18: 1013-1022.PubMedPubMed CentralView ArticleGoogle Scholar
- Longo VD, Gralla EB, Valentine JS: Superoxide dismutase activity is essential for stationary phase survival in Saccharomyces cerevisiae. Mitochondrial production of toxic oxygen species in vivo. J Biol Chem. 1996, 271: 12275-12280. 10.1074/jbc.271.21.12275.PubMedView ArticleGoogle Scholar
- Fabrizio P, Liou LL, Moy VN, Diaspro A, Valentine JS, Gralla EB, Longo VD: SOD2 functions downstream of Sch9 to extend longevity in yeast. Genetics. 2003, 163: 35-46.PubMedPubMed CentralGoogle Scholar
- Mayr B, Montminy M: Transcriptional regulation by the phosphorylation-dependent factor CREB. Nat Rev Mol Cell Biol. 2001, 2: 599-609. 10.1038/35085068.PubMedView ArticleGoogle Scholar
- Ramsay RG, Gonda TJ: MYB function in normal and cancer cells. Nat Rev Cancer. 2008, 8: 523-534. 10.1038/nrc2439.PubMedView ArticleGoogle Scholar
- Lessard J, Wu JI, Ranish JA, Wan M, Winslow MM, Staahl BT, Wu H, Aebersold R, Graef IA, Crabtree GR: An essential switch in subunit composition of a chromatin remodeling complex during neural development. Neuron. 2007, 55: 201-215. 10.1016/j.neuron.2007.06.019.PubMedPubMed CentralView ArticleGoogle Scholar
- Kitano H: Biological robustness. Nat Rev Genet. 2004, 5: 826-837.PubMedView ArticleGoogle Scholar
- m:Explorer website. [http://biit.cs.ut.ee/mexplorer/]
- Venables WN, Ripley BD: Modern Applied Statistics with S. 2003, New York, USA: Springer, 4Google Scholar
- Reimand J, Kull M, Peterson H, Hansen J, Vilo J: g:Profiler-a web-based toolset for functional profiling of gene lists from large-scale experiments. Nucleic Acids Res. 2007, 35: 193-200.View ArticleGoogle Scholar
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