Volume 12 Supplement 1

Beyond the Genome 2011

Open Access

A novel functional variant in 8q24 is associated with regulation of prostate stem cell antigen (PSCA) gene expression and bladder cancer risk

  • Yi-Ping Fu1,
  • Indu Kohaar1,
  • Adam Mumy1,
  • Wei Tang1,
  • Brian Muchmore1,
  • Patricia Porter-Gill1,
  • Luyang Liu1,
  • Jonine Figueroa2,
  • Montserrat Garcia-Closas2,
  • Dalsu Baris2,
  • Mark Purdue2,
  • Michael Thun3,
  • Demetrius Albanes2,
  • Nuria Malats4,
  • Francisco X Real4,
  • Manolis Kogevinas5,
  • Alison Johnson6,
  • Molly Schwenn7,
  • Stephen Chanock1,
  • Nathaniel Rothman2,
  • Debra Silverman2 and
  • Ludmila Prokunina-Olsson1
Genome Biology201112(Suppl 1):P4

https://doi.org/10.1186/gb-2011-12-s1-p4

Published: 19 September 2011

Background

Recent genome-wide association studies (GWAS) have identified allele T of a single nucleotide polymorphism (SNP), rs2294008, in the prostate stem cell antigen (PSCA) gene as a risk factor for bladder cancer [1, 2]. In the present study, we aimed to find additional disease-associated SNPs in the PSCA region and to explore their possible molecular function.

Methods

Based on information from the 1000 Genomes and HapMap 3 projects, we performed imputation analysis on 3,532 bladder cancer cases and 5,120 healthy controls of European ancestry from the stage 1 bladder cancer GWAS, within ±100 kb of the region flanking the GWAS signal, rs2294008. The average allele dosage and best-guess genotypes were estimated and tested for association between SNP variants and bladder cancer risk by using unconditional logistic regression. Functional follow-up studies included RNA sequencing in normal and tumor bladder samples and electrophoretic mobility shift assays to examine the potentially altered DNA-protein interactions for SNPs of interest.

Results

A total of 639 imputed and 37 genotyped SNPs within ±100 kb of the region of the original GWAS signal were tested for genetic association with bladder cancer. In these stage 1 GWAS samples, the SNP rs2294008 had a per-allele odds ratio (OR) of 1.09 (95% confidence interval (CI) = 1.02 to 1.16, P = 6.93 × 10–4). Multivariable logistic regression analysis adjusted for the study center, age, gender, smoking status and rs2294008 genotype revealed a novel associated variant, rs2978974 (OR = 1.11, 95% CI = 1.04 to 1.19, P = 1.62 × 10–3). There was low linkage disequilibrium between rs2978974 and the original GWAS signal, rs2294008 (D = 0.19, r2 = 0.02). Only individuals carrying the risk variant of both SNPs had an increased risk of bladder cancer (OR = 1.24, 95% CI = 1.13 to 1.35, P = 4.69 × 10–6) and not individuals who carried a risk variant of only one of the SNPs (P > 0.05). Stratified analysis suggested that this compound effect of rs2294008 and rs2978974 was more significant in males (OR = 1.27, P = 2.80 × 10–6) than in females (OR = 1.08, P = 0.52).

rs2978974 resides 10 kb upstream of rs2294008, is marked by an H3K4me3 signal and is in the vicinity of an androgen-receptor-binding site. Using RNA sequencing of bladder samples, we showed that rs2978974 is located within an alternative, untranslated first exon of PSCA. Using the electrophoretic mobility shift assay with nuclear proteins from LNCaP and HeLa cells, we observed that the non-risk-associated allele (G) of rs2978974, but not the risk allele (A), could bind to ELK1, a protein belonging to the ETS family of transcription factors.

Conclusions

We identified a SNP, rs2978974, in the PSCA region as a novel marker for bladder cancer susceptibility. There was a compound effect in carriers of both the rs2294008 and rs2978974 risk variants. The functional relevance of rs2978974 might be related to the loss of ELK1 regulation by the risk allele (A) and differential regulation of PSCA mRNA expression.

Authors’ Affiliations

(1)
Laboratory of Translationa Genomics, Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health
(2)
Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health
(3)
Epidemiology Research Program, American Cancer Society Atlanta
(4)
Spanish National Cancer Research Center
(5)
Centre for Research in Environmental Epidemiology
(6)
Vermont Cancer Registry Burlington
(7)
Maine Cancer Registry Augusta

References

  1. Rothman N, Garcia-Closas M, Chatterjee N, Malats N, Wu X, Figueroa JD, Real FX, Van Den Berg D, Matullo G, Baris D, Thun M, Kiemeney LA, Vineis P, De Vivo I, Albanes D, Purdue MP, Rafnar T, Hildebrandt MA, Kiltie AE, Cussenot O, Golka K, Kumar R, Taylor JA, Mayordomo JI, Jacobs KB, Kogevinas M, Hutchinson A, Wang Z, Fu YP, Prokunina-Olsson L, et al: A multi-stage genome-wide association study of bladder cancer identifies multiple susceptibility loci. Nat Genet. 2010, 42: 978-984. 10.1038/ng.687.PubMedPubMed CentralView ArticleGoogle Scholar
  2. Wu X, Ye Y, Kiemeney LA, Sulem P, Rafnar T, Matullo G, Seminara D, Yoshida T, Saeki N, Andrew AS, Dinney CP, Czerniak B, Zhang ZF, Kiltie AE, Bishop DT, Vineis P, Porru S, Buntinx F, Kellen E, Zeegers MP, Kumar R, Rudnai P, Gurzau E, Koppova K, Mayordomo JI, Sanchez M, Saez B, Lindblom A, de Verdier P, Steineck G, et al: Genetic variation in the prostate stem cell antigen gene PSCA confers susceptibility to urinary bladder cancer. Nat Genet. 2009, 41: 991-995. 10.1038/ng.421.PubMedPubMed CentralView ArticleGoogle Scholar

Copyright

© Fu et al; licensee BioMed Central Ltd. 2011

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Advertisement