- Protein family review
- Open Access
The WUS homeobox-containing (WOX) protein family
Genome Biology volume 10, Article number: 248 (2009)
The WOX genes form a plant-specific subclade of the eukaryotic homeobox transcription factor superfamily, which is characterized by the presence of a conserved DNA-binding homeodomain. The analysis of WOX gene expression and function shows that WOX family members fulfill specialized functions in key developmental processes in plants, such as embryonic patterning, stem-cell maintenance and organ formation. These functions can be related to either promotion of cell division activity and/or prevention of premature cell differentiation. The phylogenetic tree of the plant WOX proteins can be divided into three clades, termed the WUS, intermediate and ancient clade. WOX proteins of the WUS clade appear to some extent able to functionally complement other members. The specific function of individual WOX-family proteins is most probably determined by their spatiotemporal expression pattern and probably also by their interaction with other proteins, which may repress their transcriptional activity. The prototypic WOX-family member WUS has recently been shown to act as a bifunctional transcription factor, functioning as repressor in stem-cell regulation and as activator in floral patterning. Past research has mainly focused on part of the WOX protein family in some model flowering plants, such as Arabidopsis thaliana (thale cress) or Oryza sativa (rice). Future research, including so-far neglected clades and non-flowering plants, is expected to reveal how these master switches of plant differentiation and embryonic patterning evolved and how they fulfill their function.
Gene organization and evolutionary history
The eukaryotic superfamily of homeobox (HB) transcription factors is characterized by the presence of a short stretch of amino acids (60-66 residues) that folds into a DNA-binding domain termed the homeodomain, which is encoded by the HB DNA sequence [1, 2]. HB transcription factors are important regulators of developmental decisions in eukaryotes, as exemplified by the prototypic HB transcription factors, the animal HOX proteins. HOX genes were initially identified in Drosophila melanogaster by homeotic mutations that transform one body segment into another, which indicated the involvement of HOX proteins in patterning along the main body axis . HB transcription factors also occur in plants, where they have a wide variety of roles. The WUSCHEL (WUS) homeobox transcription factor is the prototypic member of the plant-specific WUS homeobox (WOX) protein family, one of a number of plant HB transcription factor families. WUS itself is expressed in the organizing-center cells of the shoot apical meristem and regulates shoot stem-cell maintenance. Families of HB transcription factors are generally distinguished by the phylogenetic relatedness of their homeodomains, and by the presence or absence of additional domains. The WOX family is distinguished by the phylogenetic relatedness of its homeodomains , as is the plant HB family Knotted related homeobox (KNOX). Other plant HB protein families are distinguished by the possession of additional domains, for example, the HD-Zip family have leucine zippers and the Zf-HD family zinc finger domains [5–7].
Phylogenetic reconstruction of protein sequences that contain the homeodomain as defined by the PFAM database [8, 9] (Figure 1a) reveals that this DNA-binding motif probably originated before the divergence of the eukaryotes . (The PFAM-defined homeodomain is the one referred to throughout this article.) The last common ancestor of all extant eukaryotes probably already harbored several HB proteins (see Figure 1a). These were subsequently subject to loss as well as expansion among different lineages and diversified in function. However, because of the short length of the homeodomain, convergent evolution (evolution leading to similar sequences that lack a common ancestor) due to structural constraints imposed by a requirement for DNA binding, for example, cannot be excluded. This might explain some surprising appearances of HB proteins from different taxonomic groups within families that otherwise are apparently specific to a certain lineage (see Figure 1a).
The phylogenetic tree of the plant WOX proteins (see Figure 1b) can be naturally divided into three clades. Arabidopsis thaliana WUS, as well as its orthologs from other flowering plant species, is located in a clade that also harbors the root apical meristem regulator WOX5 and the remainder of the WOX proteins 1-7 (Table 1); we will refer to this clade as the WUS clade. The sister clade of the WUS clade contains the A. thaliana WOX8, 9, 11 and 12 proteins; we will refer to this clade as the intermediate clade, as it is interspersed between the other two clades. Separated by the midpoint root from the two other clades is the ancient clade (probably representing the earliest diverging WOX genes), which harbors the A. thaliana WOX10, 13 and 14 proteins. It is noteworthy that only the ancient clade contains WOX sequences from green algae and from the non-vascular moss Physcomitrella patens (see Figure 1b). Therefore, at least one WOX gene must already have been present in the last common ancestor of the 'green' lineage (the lineage consisting of land plants and green algae). The longest internal branch separating the ancient clade from the remainder of the WOX family [7, 10], and the positioning of the root at this branch [7, 11], has been established before.
Whether the last common ancestor of the green lineage already possessed two WOX genes, and the gene that gave rise to the intermediate clade was subsequently lost from parts of the lineage, or whether a paralog that gave rise to these clades was established later, in the last common ancestor of vascular plants, cannot be resolved at present [7, 11, 12]. The intermediate clade contains, besides members from flowering plants, sequences from the vascular lycophyte Selaginella moellendorffii. The paralogs giving rise to this clade must therefore have already been present in the last common ancestor of vascular plants. The WUS clade contains protein sequences from flowering plants only. Analyses of organisms for which we have no genome sequence at present have demonstrated that the WUS clade is specific to seed plants and that WUS and WOX5 arose after the divergence of gymnosperms (plants bearing naked seeds) and angiosperms (plants bearing enclosed seeds) .
Characteristic structural features
The homeodomain binds DNA through a helix-turn-helix (HTH) structure. The HTH motif is characterized by two α-helices, which make intimate contacts with the DNA and are joined by a short turn. The second helix binds to DNA via a number of hydrogen bonds and hydrophobic interactions, which occur between specific side chains and the exposed bases and thymine methyl groups within the major groove of the DNA . The recognized DNA core motifs differ. Homology modeling of the plant WOX homeodomain reveals two extended loops within a generally highly conserved structure as compared with the animal HOX homeodomain (Figure 2). Such extensions are also known from other HB families, for example the ancient TALE class homeodomain family , which has a three-amino-acid loop extension between helices 1 and 2 and has important roles in plant, animal and fungal development, for example as cofactors of the HOX proteins. The position of the homeodomain within the protein varies in different members of the WOX family (Figure 3).
As well as the homeodomain, the WOX proteins contain the distinctive WUS-box motif (essentially of the form T-L-X-L-F-P-X-X, where X can be any amino acid)  that distinguishes them from other HB transcription factors. In its strict definition, this motif is present in the members of the WUS clade and is located carboxy-terminal to the homeodomain (see Figure 3). The WUS-box motif was shown to be essential for WUS function in both the regulation of the shoot stem-cell population (Box 1) and in floral patterning . The members of the WUS clade all possess the two-amino-acid motif T-L at the start of the WUS box, whereas the non-WUS WOX-family members show variation at this position. The WUS-box motif is found in the same relative position in all other members of the A. thaliana WOX family.
Several WOX proteins contain a stretch of acidic amino acids between the homeodomain and the WUS box that could potentially function as an activator domain, and/or contain a carboxy-terminal ERF-associated amphiphilic repression (EAR) domain that has been shown to be involved in transcriptional repression ( and references therein). As the EAR domain can mediate interaction with the co-repressor protein TOPLESS  (see Figure 3), the repressor activity of proteins containing EAR domains might depend on protein interaction. The recently described expanded Aux/IAA EAR domain [LI]-X-[LI]-[AG]-[LP]-[PGST]  has not been detected in any A. thaliana WOX protein. Only a relaxed form of the EAR motif, namely [LVI]-X- [LVI]-X- [LVI], can be detected in members of all three WOX subclades. This motif is not always present at the carboxyl terminus and in some cases overlaps with other domains and is present in multiple copies (for example, two in A. thaliana WOX4, 9 and WUS and three in WOX8). Simple L-X-L motifs are present in all A. thaliana WOX proteins except WOX8 and 10. Only WUS, WOX5 and WOX7 contain the carboxy-terminal EAR motif in its strict sense (see Figure 3).
Localization and function
Despite their function as transcription factors, no clear nuclear localization signal (NLS) can be predicted for any of the WOX family members (using PSORT  and PredictNLS ). Subcellular localization has been investigated so far only for some WOX-family members. WUS , WOX6/PFS2 (WOX6 is named PRETTY FEW SEEDS 2 in A. thaliana)  and WOX11  are localized to the nucleus. This nuclear localization might involve cryptic NLS motifs not detected by prediction algorithms and/or interactions with other proteins that themselves contain a NLS. The positively charged amino acids present as stretches of two or three residues throughout the homeodomain might represent such a cryptic NLS. Table 1 lists the expression domains and putative functions of WOX proteins in several plant species.
The WUS clade
The WUS and WOX5 genes are expressed in the organizing-center cells of the shoot and root apical meristem, respectively, where they are involved in the maintenance of stem-cell function (see Box 1) [17, 21, 22]. In addition to its expression in the quiescent center cells (see Box 1), WOX5 is expressed early during the initiation and outgrowth of lateral root primordia (which produce the lateral roots post-embryonically) and in the cotyledon primordia (which produce the cotyledons, flanking the shoot apical meristem) , suggesting that WOX5 also functions in these tissues. Interestingly, WOX5 and WUS were shown to be exchangeable in regulating stem-cell maintenance in shoot and root . The function of WUS and WOX5 in stem-cell maintenance was demonstrated by loss-of-function mutations. In wus loss-of-function mutants the stem cells that are maintained by signaling from the organizing center undergo differentiation, both in A. thaliana and Antirrhinum majus (snapdragon) [18, 24], Besides its role in stem-cell maintenance, WUS is involved in ovule and anther development in A. thaliana [25, 26] and fulfills a similar role in grasses . In the A. thaliana wox5 loss-of-function mutant, the root columella stem cells, which normally produce the gravity-sensing root cap cells, undergo differentiation .
WOX5 and WUS function is conserved in angiosperms, but only a single WOX5/WUS homolog is present in gymnosperms . The WOX3/PRS1 (PRESSED FLOWER 1) gene and the Zea mays (maize) orthologs NS1 and NS2 (NARROW SHEATH) regulate the recruitment of organ founder cells from the lateral domains of plant meristems and promote cell proliferation . A. thaliana WOX6/PFS2 prevents premature differentiation during formation of the integument (the structure enclosing the embryo sac) and the egg cell . An additional role for WOX6 in the response to cold stress was identified by the isolation of a mutant allele of WOX6 named hos9-1 . hos (high expression of osmotically responsive genes) mutants grow more slowly, flower later, and are more sensitive to freezing.
The WOX2 protein was shown to be required for apical patterning during A. thaliana embryo development (see Box 1) [30, 31] and is regulated by WOX8 and WOX9 (also known as STIMPY in A. thaliana). In Picea abies (Norway spruce) WOX2 expression is correlated with somatic embryogenesis [10, 32]. Interestingly, A. thaliana WOX1, WOX3 and WOX5 act redundantly with WOX2 during apical patterning . In Petunia hybrida (petunia), the WOX family member MAW (MAEWEST) combines the separate functions of A. thaliana WOX1 and WOX3 proteins in lateral organ development and prevention of organ fusion .
The ancient and the intermediate clades
The WOX8 and WOX9 genes are redundantly required for development of the basal lineage (giving rise to the hypophysis and suspensor) in the A. thaliana embryo and for regulation of WOX2 expression in the apical domain (the embryo proper) [30, 31]. Initially, WOX2 and WOX8 are coexpressed in the zygote, but during embryo development, WOX2 expression and WOX8 and WOX9 expression become restricted to the apical and basal domains, respectively . The identified WOX cascade sets up the main body axis in the embryo and regulates the localized auxin response through the auxin-transporter protein PIN1; PIN family members function as auxin-efflux carriers and are crucially involved in the establishment of directed auxin transport.
In addition to its role in embryonic patterning, WOX9 was shown to be required for shoot apical meristem maintenance  and for maintaining cell division activity during embryonic and post-embryonic development in A. thaliana , Solanum esculentum (tomato)  and P. hybrida . Transcript profiling in Brassica napus (rape) identified WOX2 and WOX9 as markers of embryogenesis , indicating that these genes serve as robust markers for (somatic) embryogenesis. The O. sativa WOX11 ortholog is expressed in the proliferating regions of both shoot and root meristems and functions in crown root development . In A. thaliana, the basal WOX-family members WOX13 and WOX14 are expressed in primary and lateral roots and floral organs, where they appear to prevent premature differentiation .
Mechanism of action
The function of most of the WOX genes studied so far can be related to either promotion of cell division and/or prevention of premature differentiation. In a number of cases, mutations in WOX genes cause non-cell-autonomous effects, suggesting that they trigger the production of intercellular signals. In A. thaliana, WUS and WOX5 function non-cell-autonomously in stem-cell maintenance , and WOX3 in organ initiation . In addition, WOX8 and WOX9 function non-cell-autonomously to regulate the apical domain during embryonic patterning . To explain the non-cell-autonomous functions, the transcriptional activity of the WOX proteins in the nucleus could trigger the production of mobile signals.
WUS activity in the apical meristem is regulated through a regulatory negative feedback loop between the WUS and CLAVATA (CLV) genes . The CLV3 peptide (which belongs to the CLE family) functions as a mobile signal and upon binding to its receptor CLV1, a receptor protein kinase, results in the repression of WUS transcription. A WUS-AGAMOUS negative feedback loop is involved in floral stem-cell maintenance. WUS activates transcription of the floral organ identity gene AGAMOUS, and in turn AGAMOUS (a MADS-box HB transcription factor) is able to repress WUS transcription [41, 42]. Recently, a putative negative feedback loop involving another member of the CLE family of peptide signals, CLE40, and the ACR4 receptor-like kinase was identified as regulating WOX5 activity in the A. thaliana root in a similar way to the regulation of WUS by CLV3 and CLV1 , suggesting that similar regulatory mechanisms are responsible for stem-cell maintenance in the shoot and root.
WUS has been shown to directly repress the transcription of several ARR-A genes, which encode negative regulators of signaling by the plant hormone cytokinin. AAR-A proteins probably act by competing for phosphorylation with the ARR-B positive regulators of cytokinin signaling - phosphorylation is required for activation of ARR-B transcription factors . Interestingly, WUS can act as both a repressor and an activator of gene expression, and the WUS-box motif is essential for both functions . In contrast, the carboxy-terminal EAR domain, found in WUS, WOX5 and WOX7 (as noted previously), is not essential for transcriptional repression of WUS and probably only enhances the repressor activity . The repressor activity of WUS could be partly mediated by the co-repressor protein TOPLESS , which interacts with WUS via the carboxy-terminal EAR motif . The EAR domain of WOX5 , however, was shown to act as a repressor in vitro . Despite not possessing the EAR repressor motif, WOX11 and WOX3 seem to function as repressors; O. sativa WOX11 directly represses transcription of RR2, which encodes an ARR-A-type negative regulator of the cytokinin response , and WOX3 was shown to repress the gene for the YABBY transcription factor (YAB3) during leaf development in O. sativa  and functions as repressor in vitro . Therefore, transcriptional repression appears to be a common mode of action for WOX proteins, but both the type of target gene and the functional domain(s) involved in repression appear to differ. Future research will need to unravel which of these motifs have a regulatory function.
The WOX proteins regulate key developmental processes in plants. However, only a subset of the family members has yet been characterized in detail in a small set of seed plants. It will be important to investigate the function of all family members, including the little studied ancient WOX clade, in a broad range of plant species to understand how the distinct functions have evolved. To understand the mechanism by which the WOX family members regulate the expression of their target genes, comprehensive expression analyses are required. While the WOX proteins seem to be able to directly repress transcription of their target genes, the role of the EAR repressor motif and/or interaction with TOPLESS-like co-repressors is still unclear.
In flowering plants, often only a small number of cells express a given WOX gene and these cells are relatively inaccessible inside surrounding tissue. Technological developments that enable 'omics' approaches with a limited amount of starting material should therefore prove beneficial. Current developments in fluorescence-activated cell sorting enable the isolation of cells expressing specific WOX genes  for transcriptome studies. The dominant gametophytic generation and the less complex morphology and easier accessibility of stem cells in non-seed plants will also enable future insights, especially into the function of members of the ancient and intermediate clade [48, 49].
Gehring WJ, Muller M, Affolter M, Percival-Smith A, Billeter M, Qian YQ, Otting G, Wuthrich K: The structure of the homeodomain and its functional implications. Trends Genet. 1990, 6: 323-329. 10.1016/0168-9525(90)90253-3.
Gehring WJ, Qian YQ, Billeter M, Furukubo-Tokunaga K, Schier AF, Resendez-Perez D, Affolter M, Otting G, Wuthrich K: Homeodomain-DNA recognition. Cell. 1994, 78: 211-223. 10.1016/0092-8674(94)90292-5.
Gehring WJ: Exploring the homeobox. Gene. 1993, 135: 215-221. 10.1016/0378-1119(93)90068-E.
Haecker A, Gross-Hardt R, Geiges B, Sarkar A, Breuninger H, Herrmann M, Laux T: Expression dynamics of WOX genes mark cell fate decisions during early embryonic patterning in Arabidopsis thaliana. Development. 2004, 131: 657-668. 10.1242/dev.00963.
Ariel FD, Manavella PA, Dezar CA, Chan RL: The true story of the HD-Zip family. Trends Plant Sci. 2007, 12: 419-426. 10.1016/j.tplants.2007.08.003.
Riano-Pachon DM, Ruzicic S, Dreyer I, Mueller-Roeber B: PlnTFDB: an integrative plant transcription factor database. BMC Bioinformatics. 2007, 8: 42-10.1186/1471-2105-8-42.
Richardt S, Lang D, Frank W, Reski R, Rensing SA: PlanTAPDB: a phylogeny-based resource of plant transcription associated proteins. Plant Physiol. 2007, 143: 1452-1466. 10.1104/pp.107.095760.
Finn RD, Tate J, Mistry J, Coggill PC, Sammut SJ, Hotz HR, Ceric G, Forslund K, Eddy SR, Sonnhammer EL, Bateman A: The Pfam protein families database. Nucleic Acids Res. 2008, 36: D281-D288. 10.1093/nar/gkm960.
Pfam: Family: Homeobox (PF00046). [http://pfam.sanger.ac.uk/family?Homeobox]
Palovaara J, Hakman I: Conifer WOX-related homeodomain transcription factors, developmental consideration and expression dynamic of WOX2 during Picea abies somatic embryogenesis. Plant Mol Biol. 2008, 66: 533-549. 10.1007/s11103-008-9289-5.
Deveaux Y, Toffano-Nioche C, Claisse G, Thareau V, Morin H, Laufs P, Moreau H, Kreis M, Lecharny A: Genes of the most conserved WOX clade in plants affect root and flower development in Arabidopsis. BMC Evol Biol. 2008, 8: 291-10.1186/1471-2148-8-291.
Nardmann J, Reisewitz P, Werr W: Discrete shoot and root stem cell-promoting WUS/WOX5 functions are an evolutionary innovation of angiosperms. Mol Biol Evol. 2009, 26: 1745-1755. 10.1093/molbev/msp084.
Mukherjee K, Burglin TR: Comprehensive analysis of animal TALE homeobox genes: new conserved motifs and cases of accelerated evolution. J Mol Evol. 2007, 65: 137-153. 10.1007/s00239-006-0023-0.
Ikeda M, Mitsuda N, Ohme-Takagi M: Arabidopsis WUSCHEL is a bifunctional transcription factor that acts as a repressor in stem cell regulation and as an activator in floral patterning. Plant Cell. 2009, 6: 6-
Paponov IA, Teale W, Lang D, Paponov M, Reski R, Rensing SA, Palme K: The evolution of nuclear auxin signalling. BMC Evol Biol. 2009, 9: 126-10.1186/1471-2148-9-126.
Nakai K, Horton P: Computational prediction of subcellular localization. Methods Mol Biol. 2007, 390: 429-466. full_text.
Nair R, Rost B: LOCnet and LOCtarget: sub-cellular localization for structural genomics targets. Nucleic Acids Res. 2004, 32: W517-W521. 10.1093/nar/gkh441.
Mayer KF, Schoof H, Haecker A, Lenhard M, Jurgens G, Laux T: Role of WUSCHEL in regulating stem cell fate in the Arabidopsis shoot meristem. Cell. 1998, 95: 805-815. 10.1016/S0092-8674(00)81703-1.
Park SO, Zheng Z, Oppenheimer DG, Hauser BA: The PRETTY FEW SEEDS2 gene encodes an Arabidopsis homeo domain protein that regulates ovule development. Development. 2005, 132: 841-849. 10.1242/dev.01654.
Zhao Y, Hu Y, Dai M, Huang L, Zhou DX: The WUSCHEL-related homeobox gene WOX11 is required to activate shoot-borne crown root development in rice. Plant Cell. 2009, 21: 736-748. 10.1105/tpc.108.061655.
Sarkar AK, Luijten M, Miyashima S, Lenhard M, Hashimoto T, Nakajima K, Scheres B, Heidstra R, Laux T: Conserved factors regulate signalling in Arabidopsis thaliana shoot and root stem cell organizers. Nature. 2007, 446: 811-814. 10.1038/nature05703.
Kamiya N, Nagasaki H, Morikami A, Sato Y, Matsuoka M: Isolation and characterization of a rice WUSCHEL-type homeobox gene that is specifically expressed in the central cells of a quiescent center in the root apical meristem. Plant J. 2003, 35: 429-441. 10.1046/j.1365-313X.2003.01816.x.
Ditengou FA, Teale WD, Kochersperger P, Flittner KA, Kneuper I, Graaff van der E, Nziengui H, Pinosa F, Li X, Nitschke R, Laux T, Palme K: Mechanical induction of lateral root initiation in Arabidopsis thaliana. Proc Natl Acad Sci USA. 2008, 105: 18818-18823. 10.1073/pnas.0807814105.
Kieffer M, Stern Y, Cook H, Clerici E, Maulbetsch C, Laux T, Davies B: Analysis of the transcription factor WUSCHEL and its functional homologue in Antirrhinum reveals a potential mechanism for their roles in meristem maintenance. Plant Cell. 2006, 18: 560-573. 10.1105/tpc.105.039107.
Gross-Hardt R, Lenhard M, Laux T: WUSCHEL signaling functions in interregional communication during Arabidopsis ovule development. Genes Dev. 2002, 16: 1129-1138. 10.1101/gad.225202.
Deyhle F, Sarkar AK, Tucker EJ, Laux T: WUSCHEL regulates cell differentiation during anther development. Dev Biol. 2007, 302: 154-159. 10.1016/j.ydbio.2006.09.013.
Nardmann J, Werr W: The shoot stem cell niche in angiosperms: expression patterns of WUS orthologues in rice and maize imply major modifications in the course of mono- and dicot evolution. Mol Biol Evol. 2006, 23: 2492-2504. 10.1093/molbev/msl125.
Shimizu R, Ji J, Kelsey E, Ohtsu K, Schnable PS, Scanlon MJ: Tissue specificity and evolution of meristematic WOX3 function. Plant Physiol. 2009, 149: 841-850. 10.1104/pp.108.130765.
Zhu J, Shi H, Lee BH, Damsz B, Cheng S, Stirm V, Zhu JK, Hasegawa PM, Bressan RA: An Arabidopsis homeodomain transcription factor gene, HOS9, mediates cold tolerance through a CBF-independent pathway. Proc Natl Acad Sci USA. 2004, 101: 9873-9878. 10.1073/pnas.0403166101.
Breuninger H, Rikirsch E, Hermann M, Ueda M, Laux T: Differential expression of WOX genes mediates apical-basal axis formation in the Arabidopsis embryo. Dev Cell. 2008, 14: 867-876. 10.1016/j.devcel.2008.03.008.
Wu X, Chory J, Weigel D: Combinations of WOX activities regulate tissue proliferation during Arabidopsis embryonic development. Dev Biol. 2007, 309: 306-316. 10.1016/j.ydbio.2007.07.019.
Palovaara J, Hakman I: WOX2 and polar auxin transport during spruce embryo pattern formation. Plant Signal Behav. 2009, 4: 153-155.
Vandenbussche M, Horstman A, Zethof J, Koes R, Rijpkema AS, Gerats T: Differential recruitment of WOX transcription factors for lateral development and organ fusion in Petunia and Arabidopsis. Plant Cell. 2009, 21: 2269-2283. 10.1105/tpc.109.065862.
Peret B, De Rybel B, Casimiro I, Benkova E, Swarup R, Laplaze L, Beeckman T, Bennett MJ: Arabidopsis lateral root development: an emerging story. Trends Plant Sci. 2009, 14: 399-408. 10.1016/j.tplants.2009.05.002.
Wu X, Dabi T, Weigel D: Requirement of homeobox gene STIMPY/WOX9 for Arabidopsis meristem growth and maintenance. Curr Biol. 2005, 15: 436-440. 10.1016/j.cub.2004.12.079.
Lippman ZB, Cohen O, Alvarez JP, Abu-Abied M, Pekker I, Paran I, Eshed Y, Zamir D: The making of a compound inflorescence in tomato and related nightshades. PLoS Biol. 2008, 6: e288-10.1371/journal.pbio.0060288.
Rebocho AB, Bliek M, Kusters E, Castel R, Procissi A, Roobeek I, Souer E, Koes R: Role of EVERGREEN in the development of the cymose petunia inflorescence. Dev Cell. 2008, 15: 437-447. 10.1016/j.devcel.2008.08.007.
Malik MR, Wang F, Dirpaul JM, Zhou N, Polowick PL, Ferrie AM, Krochko JE: Transcript profiling and identification of molecular markers for early microspore embryogenesis in Brassica napus. Plant Physiol. 2007, 144: 134-154. 10.1104/pp.106.092932.
Deveaux Y, Toffano-Nioche C, Claisse G, Thareau V, Morin H, Laufs P, Moreau H, Kreis M, Lecharny A: Genes of the most conserved WOX clade in plants affect root and flower development in Arabidopsis. BMC Evol Biol. 2008, 8: 291-10.1186/1471-2148-8-291.
Schoof H, Lenhard M, Haecker A, Mayer KF, Jurgens G, Laux T: The stem cell population of Arabidopsis shoot meristems in maintained by a regulatory loop between the CLAVATA and WUSCHEL genes. Cell. 2000, 100: 635-644. 10.1016/S0092-8674(00)80700-X.
Lenhard M, Bohnert A, Jurgens G, Laux T: Termination of stem cell maintenance in Arabidopsis floral meristems by interactions between WUSCHEL and AGAMOUS. Cell. 2001, 105: 805-814. 10.1016/S0092-8674(01)00390-7.
Lohmann JU, Hong RL, Hobe M, Busch MA, Parcy F, Simon R, Weigel D: A molecular link between stem cell regulation and floral patterning in Arabidopsis. Cell. 2001, 105: 793-803. 10.1016/S0092-8674(01)00384-1.
Stahl Y, Wink RH, Ingram GC, Simon R: A signaling module controlling the stem cell niche in Arabidopsis root meristems. Curr Biol. 2009, 19: 909-914. 10.1016/j.cub.2009.03.060.
Leibfried A, To JP, Busch W, Stehling S, Kehle A, Demar M, Kieber JJ, Lohmann JU: WUSCHEL controls meristem function by direct regulation of cytokinin-inducible response regulators. Nature. 2005, 438: 1172-1175. 10.1038/nature04270.
Long JA, Ohno C, Smith ZR, Meyerowitz EM: TOPLESS regulates apical embryonic fate in Arabidopsis. Science. 2006, 312: 1520-1523. 10.1126/science.1123841.
Dai M, Hu Y, Zhao Y, Liu H, Zhou DX: A WUSCHEL-LIKE HOMEOBOX gene represses a YABBY gene expression required for rice leaf development. Plant Physiol. 2007, 144: 380-390. 10.1104/pp.107.095737.
Yadav RK, Girke T, Pasala S, Xie M, Reddy GV: Gene expression map of the Arabidopsis shoot apical meristem stem cell niche. Proc Natl Acad Sci USA. 2009, 106: 4941-4946. 10.1073/pnas.0900843106.
Harrison CJ, Roeder AH, Meyerowitz EM, Langdale JA: Local cues and asymmetric cell divisions underpin body plan transitions in the moss Physcomitrella patens. Curr Biol. 2009, 19: 461-471. 10.1016/j.cub.2009.02.050.
Mosquna A, Katz A, Decker EL, Rensing SA, Reski R, Ohad N: Regulation of stem cell maintenance by the Polycomb protein FIE has been conserved during land plant evolution. Development. 2009, 136: 2433-2444. 10.1242/dev.035048.
Katoh K, Kuma K, Toh H, Miyata T: MAFFT version 5: improvement in accuracy of multiple sequence alignment. Nucleic Acids Res. 2005, 33: 511-518. 10.1093/nar/gki198.
Howe K, Bateman A, Durbin R: QuickTree: building huge neighbour-joining trees of protein sequences. Bioinformatics. 2002, 18: 1546-1547. 10.1093/bioinformatics/18.11.1546.
Frickenhaus S, Beszteri B: Quicktree-SD, Software Developed by AWI-Bioinformatics. 2008, [http://hdl.handle.net/10013/epic.33164]
Ronquist F, Huelsenbeck JP: MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics. 2003, 19: 1572-1574. 10.1093/bioinformatics/btg180.
Longo A, Guanga GP, Rose RB: Structural basis for induced fit mechanisms in DNA recognition by the Pdx1 homeodomain. Biochemistry. 2007, 46: 2948-2957. 10.1021/bi060969l.
Wang Y, Addess KJ, Chen J, Geer LY, He J, He S, Lu S, Madej T, Marchler-Bauer A, Thiessen PA, Zhang N, Bryant SH: MMDB: annotating protein sequences with Entrez's 3D-structure database. Nucleic Acids Res. 2007, 35: D298-D300. 10.1093/nar/gkl952.
PPG: the Protein Picture Generator. [http://bioserv.rpbs.jussieu.fr/PPG]
Arnold K, Bordoli L, Kopp J, Schwede T: The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling. Bioinformatics. 2006, 22: 195-201. 10.1093/bioinformatics/bti770.
The writing of this review was supported by the Federal Ministry of Education and Research (BMBF grant FRISYS 0313921 to TL and SAR), by the Excellence Initiative of the German Federal and State Governments (EXC 294 to TL and SAR) and by the German Research Foundation (DFG, SFB592 to TL).
About this article
Cite this article
van der Graaff, E., Laux, T. & Rensing, S.A. The WUS homeobox-containing (WOX) protein family. Genome Biol 10, 248 (2009). https://doi.org/10.1186/gb-2009-10-12-248
- Shoot Apical Meristem
- Main Body Axis
- Ancient Clade
- Intermediate Clade
- Root Apical Meristem Regulator