Fig. 5

Hemin alters chromatin modifications at promoters with decreased H3K27ac and H3K4me3 levels. A–C H3K4me1, H3K4me2, H3K4me3, and H3K27ac ChIP-Rx signals in HEK293T cells treated with or without hemin. Track examples at the NPM1 (A) and TFRC (B) loci are shown. As shown in metaplots (C), hemin treatment globally reduced H3K4me3 and H3K27ac levels at hemin-bound promoters. D, E Scatterplots of the log₂ fold changes of H3K4me3 signals versus hemin binding signals (D) or the log₂ fold changes of H3K27ac signals (E) after hemin treatment. The majority of hemin-bound promoters showed decreased H3K4me3 signals (D), and most of them were accompanied by decreased H3K27ac signals (E). F Heatmap and metaplot analyses of RBBP5 and P300 ChIP-Rx signals in the presence or absence of hemin. Hemin treatment impairs RBBP5 and P300 occupancy at hemin-bound promoters (N=5858)