Skip to main content

Table 1 Genome assembly statistics for two Eucalyptus species and A. hypogaea. We calculated the assembly statistics using Quast. CLR-based assemblies were 3-cycle polished as detailed in “Methods”. Results are based on the purged assemblies (see “Methods”).

From: LeafGo: Leaf to Genome, a quick workflow to produce high-quality de novo plant genomes using long-read sequencing technology

Plants Type Sized (Mb), ≥ 1 Mb|total No. contigsd, ≥ 1 Mb|total N50 (Mb)/L50 N90 (Mb)/L90 Longest contig (Mb) Alternative size (Mb)
E. rudisa HiFi (~ 40×) 531|549 26|331 36.0/7 7.3/15 61.8 425
CLR (~ 50×) 506|518 44|138 16.3/11 5.2/30 33.7 399
E. camaldulensisb HiFi (~ 51×) 525|532 14|149 41.4/5 23.2/12 69.1 520
CLR (~ 230×) 516|523 28|77 29.3/7 8.5/19 58.1 570
A. hypogaeac HiFi (~ 74×) 2,564|2,623 114|1417 42.3/22 10.37/69 90.3 51
  1. aE. rudis unknown genome size. Coverage estimated based on assembly size
  2. bE. camaldulensis reference genome size, 558.6 Mb (AC: GCA_014182705.1)
  3. cA. hypogaea reference genome size, 2557 Mb (AC: GCF_003086295.2)
  4. N50 the smallest length contig at which the cumulative contig lengths equal to 50% of the assembled size, L50 N50 contig count, N90 the smallest length contig at which the cumulative contig lengths equal to 90% of the assembled size, L90 N90 contig count
  5. dMetric calculated based on (1) minimum contig length cut-off of 1Mb or (2) no cut-off