Fig. 4
From: Retrotransposons as pathogenicity factors of the plant pathogenic fungus Botrytis cinerea
BcGypsy3 promotes host plant infection. a BcGypsy3 mRNA expression in single strand (ss)BcGypsy3 (#4, #49, #50, #51), double strand (ds)BcGypsy3 (#5, #6, #56, #57) or empty vector (EV) (#18) transformants with the BcGypsy3 transgene inserted in the BcniaD locus of D08_H24. Further, randomly inserted ssBcGypsy3 (randBcGypsy3) tranformants #1, #3 into the D08_H24 genome, as well as the wild type strains D08_H24 and B05.10 are shown. Data points represent three biological replicates. A significant difference as indicated by letters was tested by one-way ANOVA using Tukey HSD test with p < 0.05. b Pathogenicity assay with dropped spore suspension of BcGypsy3 transformants on tomato leaves quantifying lesion area of > 20 infection sites at 48 hpi. A significant difference as indicated by letters was tested by one-way ANOVA using Tukey HSD test with p < 0.05. c Pathogenicity assay with agar plugs of randBcGypsy3 transformants on tomato leaves quantifying lesion area of minimum 8 infection sites at 48 hpi. Significant difference was tested by two-sided Student’s t test. For b and c, similar results were obtained in two independent infection experiments. d BcsRNA-seq analysis at the BcGypsy3 transgene locus representing ssBcGypsy3 #51, dsBcGypsy3 #56, randBcGypsy3 #1 or EV #18 transformants with blue bars indicate sense and red bars antisense reads. Table shows normalized read counts (RPM) of BcGypsy3 BcsRNAs. e On top, stem-loop RT-PCR showed BcsRNA3.1, BcsRNA3.2 and BcsRNA20 expression in the randBcGypsy3 #1. BcGypsy3 mRNA expression and BcTub were used as controls. On the bottom, qRT-PCR of S. lycopersicum target mRNA expression after water treatment (mock), infection with D08_H24 wt or randBcGypsy3 #1. Each data point represents a biological replicate. f On the top, scheme of RNA-seq experiment infecting A. thaliana either with D08_H24 wt or randBcGypsy3 #1 comprising 12 biological replicates for each treatment. On the bottom, heat map showing differentially expressed A. thaliana genes comparing infection with D08_H24 wt or randBcGypsy3 #1