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Author Correction: CRISPRi enables isoform-specific loss-of-function screens and identification of gastric cancer-specific isoform dependencies

The Original Article was published on 26 January 2021

Correction to: Genome Biol (2021) 22:47

https://doi.org/10.1186/s13059-021-02266-6

Following publication of the original paper [1], the authors reported an error. In Fig. 1c we have mistakenly mislabeled the sgRNAs. The corrected Fig. 1 is given in this correction article.

Fig. 1
figure1

CRISPRi as a tool for inhibition of specific promoter-driven transcript isoforms. a Structure of the HNF4A gene. Isoforms P1 and P2 are marked. CAGE-Seq peaks from the FANTOM project [19] are shown in the bottom panel. b qRT-PCR quantification of HNF4A transcript P1 following CRISPRi-mediated suppression of transcript P1 or P2. Data is shown as mean ± SD, n = 2. pValue is calculated using two-tailed unpaired t test (***p ≤ 0.001). c qRT-PCR quantification of HNF4A transcript P2 following CRISPRi-mediated suppression of transcript P1 or P2. Data is shown as mean ± SD, n = 2. pValue is calculated using two-tailed unpaired t test (***p ≤ 0.001). d Structure of the IMP3 gene. Isoforms P1 and P2 are marked. CAGE-Seq peaks from the FANTOM project are shown in the bottom panel. e Violin plot showing IMP3 dependency following CRISPRi-mediated suppression of different isoforms in GC cell lines. Dots represent individual sgRNAs targeting the indicated IMP3 transcript isoform. pValue is calculated using two-tailed unpaired t test (****p ≤ 0.0001, *p ≤ 0.05). f Distribution of sgRNAs targeting different transcript isoforms of 55 pan cell-essential transcripts. Green, negative control sgRNAs. Purple, sgRNAs targeting the highest expressed (based on RNA-Seq) transcript isoform. Red, sgRNAs targeting the low expressed (based on RNA-Seq) transcript isoform

Reference

  1. 1.

    Davies R, Liu L, Taotao S, et al. CRISPRi enables isoform-specific loss-of-function screens and identification of gastric cancer-specific isoform dependencies. Genome Biol. 2021;22:47 https://doi.org/10.1186/s13059-021-02266-6.

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Correspondence to Patrick Tan or Joseph Rosenbluh.

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Davies, R., Liu, L., Taotao, S. et al. Author Correction: CRISPRi enables isoform-specific loss-of-function screens and identification of gastric cancer-specific isoform dependencies. Genome Biol 22, 88 (2021). https://doi.org/10.1186/s13059-021-02314-1

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