Fig. 2

Gene expression dynamics in the germline. a Genes expressed at significantly lower levels in oocytes relative to meiosis (circled) or mitosis (Additional file 1: Figure S10) largely comprise ribosomal protein coding genes; rRNA and tRNA biosynthetic genes are also down-regulated in oocytes (Additional file 1: Figure S10). b Transcript abundance in oocytes relative to the meiotic region is inversely proportional to the density of UUGUU motifs in 3′UTRs. Oocyte vs. mitosis comparisons are similar (Additional file 1: Figure S11). c Expression from the X chromosome is dramatically lower in male germline and in the mitotic region of adult hermaphrodites. X-linked transcripts accumulate in oocytes, but to lower levels than autosomal transcripts, which show relatively even expression throughout the germline (Additional file 1: Figure S8). d Major 3′UTR isoforms of genes with significantly higher abundance in meiosis and oocytes relative to mitosis contain a greater number of predicted GLD-1 binding motifs. e Differential isoform abundance between oocytes and mitosis (this study) correlates with expression fold-changes of GLD-1 targets in a cgh-1 temperature-sensitive mutant vs. wild type (data from Scheckel et al. [23]). Our data revealed that fold-changes for GLD-1 targets (left), but not other transcripts (right), correlate with the level of stabilization by CGH-1 observed by Scheckel et al. when comparing expression levels in meiosis (shown here) or oocytes (Additional file 1: Figure S9) relative to mitosis