- Open Access
Intron retention enhances gene regulatory complexity in vertebrates
- Ulf Schmitz1, 2View ORCID ID profile,
- Natalia Pinello1, 2, 3,
- Fangzhi Jia1, 2,
- Sultan Alasmari4,
- William Ritchie5,
- Maria-Cristina Keightley4,
- Shaniko Shini6,
- Graham J. Lieschke4,
- Justin J-L Wong†1, 2, 3 and
- John E. J. Rasko†1, 2, 7, 8Email author
© The Author(s). 2017
Received: 9 October 2017
Accepted: 13 October 2017
Published: 16 November 2017
While intron retention (IR) is now widely accepted as an important mechanism of mammalian gene expression control, it remains the least studied form of alternative splicing. To delineate conserved features of IR, we performed an exhaustive phylogenetic analysis in a highly purified and functionally defined cell type comprising neutrophilic granulocytes from five vertebrate species spanning 430 million years of evolution.
Our RNA-sequencing-based analysis suggests that IR increases gene regulatory complexity, which is indicated by a strong anti-correlation between the number of genes affected by IR and the number of protein-coding genes in the genome of individual species. Our results confirm that IR affects many orthologous or functionally related genes in granulocytes. Further analysis uncovers new and unanticipated conserved characteristics of intron-retaining transcripts. We find that intron-retaining genes are transcriptionally co-regulated from bidirectional promoters. Intron-retaining genes have significantly longer 3′ UTR sequences, with a corresponding increase in microRNA binding sites, some of which include highly conserved sequence motifs. This suggests that intron-retaining genes are highly regulated post-transcriptionally.
Our study provides unique insights concerning the role of IR as a robust and evolutionarily conserved mechanism of gene expression regulation. Our findings enhance our understanding of gene regulatory complexity by adding another contributor to evolutionary adaptation.
Most multi-exon genes (~95%) have more than one alternative splice form due to exon skipping/inclusion, alternative 3′ and 5′ splice site selection, or through intron retention (IR) . Of these modes of alternative splicing, IR is unique as it does typically not contribute to proteomic diversity. IR affects transcripts from up to three-quarters of multi-exonic genes in mammals, yet remains the least understood mode of alternative splicing [2–5].
IR occurs physiologically when the splicing machinery fails to excise introns from primary messenger RNA (mRNA) transcripts leading to the inclusion of premature termination codons (PTCs) in most intron-retaining transcripts . As a consequence, intron-retaining mRNA transcripts are susceptible to degradation via nonsense-mediated decay (NMD) [6, 7]. Thus, IR can reduce gene expression at the post-transcriptional level and thereby imposes an additional level of gene regulation. Indeed, we have shown previously that IR-triggered NMD in differentiating myeloid cells reduces the abundance of cognate RNA and proteins . In this context, several studies have subsequently reported transcripts with included introns detained in the nucleus and not susceptible to NMD [2, 8–12]. Additionally, there are several alternative hypotheses concerning the biological functions of IR [6, 13]. Intron-retaining transcripts may act as sentinel RNAs ready to be spliced and translated on demand, thereby inducing more rapid protein production than de novo transcription and translation [5, 10, 14]. Other downstream effects of IR include the synthesis of novel peptides or protein isoforms, the suppression of protein and/or non-coding RNA (ncRNA) synthesis and the regulation of nuclear mRNA export [2, 3, 15–17]. Normal regulation of IR is essential for physiological cell functions including differentiation capacity and aberrant IR leads to human diseases including cancer [18–21].
Retained introns are known to contain, on average, a higher density of GC nucleotides and are shorter in length compared to non-retained introns [2, 3]. We and others have demonstrated that IR is a conserved mechanism that affects functionally related genes in humans and mice [2, 3, 8]. Although IR has been shown to be conserved across several vertebrate species at a tissue level, such as in the nervous system and brain , a thorough analysis of IR in a highly purified and functionally defined cell type has been lacking.
Genomic characteristics of intron-retaining mammalian and vertebrate species
Genome size (GB)
Investigation of IR in a cell type with conserved function across diverse species can potentially reveal unique characteristics, which would otherwise be masked when studying mixed populations of cells . Neutrophilic granulocytes offer a discrete, well-defined cell type with phylogenetically conserved functions that serve as an excellent exemplar to study mechanisms of gene expression control. They are the most abundant cells of the innate immune system and consistently exhibit potent anti-microbial defenses since before the evolutionary divergence of teleost fish . Our experimental design provides a tightly controlled model that allowed us to examine the relationship between IR and transcriptomic complexity.
We demonstrate that IR affects many orthologous or functionally related genes and that intron-retaining transcripts have very similar characteristics in all species investigated. For example, we found that intron-retaining genes are transcriptionally regulated from bidirectional promoters. The strong anti-correlation between the number of genes affected by IR and the number of protein-coding genes in the genome of individual species suggests that IR provides a mechanism of enhancing transcriptomic complexity.
It is unknown whether IR acts independently of other post-transcriptional mechanisms of gene expression control. In examining the relationship between IR and microRNA (miRNA)-mediated gene regulation we found that intron-retaining genes have significantly longer 3′ UTR sequences that are enriched for miRNA binding sites. Our results suggest that IR is an evolutionary well-conserved form of alternative splicing that orchestrates post-transcriptional gene expression control.
The function of IR is conserved over 430 million years
We have shown previously that IR affects similar biological processes during hematopoietic differentiation in human and mouse . To study the functional conservation of IR events in species spanning 430 million years, we identified IR in terminally differentiated granulocytes using the IRFinder algorithm  (see “Methods”). For every intron the algorithm computes the IR ratio (in the range of 0–1), which is an approximation of the proportion of total transcripts that retained the given intron. More specifically, the IR ratio is the ratio of the median read coverage of the intron to that of its flanking exons. We defined a threshold for the IR ratio (IR_ratio = 0.1) in order to consider only biologically meaningful cases of IR for further analysis.
Next, we clustered all known orthologous genes based on their IR pattern in the five species under investigation. We found gene clusters in which IR events are exclusive to one particular species. While these species-specific clusters represent distinct sets of genes, annotation enrichment analysis revealed that similar terms are over-represented in association with all of these gene sets, as well as those in which the IR pattern is similar in several or all species (Additional file 2: Figure S1). This suggests that IR is a global control mechanism affecting functionally conserved biological processes independent of specific effector genes. Although we note that mutually enriched terms are rather general, they differ from terms that were found enriched in other studies of IR in purified cells (Additional file 2: Table S1).
Interestingly, functionally enriched classes of genes include phosphoproteins, kinases, and genes involved in alternative splicing, i.e. groups of intron-retaining genes that realize a multitude of gene expression and protein activity control mechanisms. Consistent with this functional enrichment, Theilgaard-Mönch et al. observed an overall decline of proliferative and general cellular activity during terminal granulocytic differentiation . This is plausible given that IR typically induces NMD and thereby negatively affects widespread mRNA and protein expression [3, 28]. Analogous to these observations is a recent finding that shows decreasing IR ratios associated with increasing levels of fully spliced mRNAs during T cell activation, to facilitate a prompt cellular response to extracellular stimuli .
In all species there are large numbers of intron-retaining genes with functions that are relevant to organelle lumina, most commonly the nuclear lumen. This result confirms our previous observations concerning the importance of IR in the control of granulocyte nuclear morphology . The organelle lumina group includes genes that we also identified in our previous study in mouse and human granulocytes , e.g. Ddx5 (all species), Ddx3x, Lbr, Atf4, Hspa5, Ing4 (human, mouse, dog, chicken), Dnmt3a (mouse, dog, chicken), Hnrnpa2b1 (human, dog), Lmnb2 (mouse, chicken), and Lmnb1 (mouse).
Other well-conserved classes of intron-retaining gene orthologs include: kinases (e.g. Cdkn1a, Cdk9/10/13, Map2k2, Sik1), RNA-binding proteins (e.g. Upf1, Dhx58, Ddx17, Upf3b); ATP binding proteins (e.g. Ddx5, Ddx3x, Hspa5, Eif4a1, Ddx39, Atp2a3, Adrbk1); and protein transporters (Hspa5, Hsp90aa1, Hsp90ab1) (see Additional file 3).
We conclude that although specific intron-retaining genes may vary between species, they are conserved in functional clusters. Our results suggest that IR is a function-centric rather than gene-centric mechanism of coordinating gene expression (Fig. 1c). Nonetheless, there are surprisingly large numbers of gene orthologs (n = 86) in which IR-mediated gene regulation is conserved in the granulocytes of all five species under investigation, i.e. species that shared a common ancestral genome sequence 430 million years ago (Fig. 1b). The number of conserved intron-retaining genes is even tenfold higher among the placental mammalian species (human, mouse, dog), which share as many as 873 intron-retaining gene orthologs. The non-mammalian vertebrates share 706 intron-retaining gene orthologs (Fig. 1b). We also analyzed conservation of IR on a per-intron basis by determining orthologs of retained introns in the other species. The results suggest that although IR on a per-intron basis is less conserved, conservation is still remarkable among the mammalian species (Additional file 2: Figure S2b).
In summary, our data indicate that IR is a well-conserved mechanism of process- or function-centric gene regulation in mammalian and vertebrate species, affecting a large number of orthologous and functionally related genes.
IR preserves functional complexity in species with fewer genes
It has previously been shown that the frequency of alternative splicing events reduces with evolutionary distance from primates . However, our observations of IR events in the investigated vertebrates contradict this observed trend (Fig. 1a). To determine whether retained introns, acting as gene expression control elements, preserve complexity in vertebrate species, we compared the fractions of intron-retaining genes in each species with the number of protein-coding genes in their genome. A strong anti-correlation exists between these two variables (r = –0.95, Pearson correlation; Fig. 1c). In contrast to other forms of alternative splicing that introduce proteomic complexity to the cell [29–32], our data indicate that IR introduces transcriptomic complexity in species with lower numbers of protein-coding genes. The most extreme example in our study is chicken in which > 40% of the 8911 expressed genes (fragments per kilobase of transcript per million mapped reads [FPKM] ≥ 1) in granulocytes possess retained introns, the largest fraction in all species investigated. Chicken has by far the smallest number of protein-coding genes among the five species, with a total of 15,508. On the other end of the spectrum is zebrafish, in which 7.8% of the 768 expressed genes contain retained introns, which is a relatively small fraction in a genome that has evolved complexity via a large number of protein-coding genes (n = 25,642). While IR anti-correlates with the number of protein-coding genes in a genome, the correlation does not hold when we instead compare IR to the number of expressed genes. Our observations indicate transcriptional control is the dominant mechanism of gene expression control in cases where few genes are expressed (zebrafish). Post-transcriptional control of gene expression including IR is more dominant in cases where many genes are expressed (chicken). In order to reinforce this thesis, we extracted the number of transcriptional regulators (transcription factors, transcription co-factors, and chromatin remodeling factors) for each species from the AnimalTFDB database  and found that this number anti-correlates (r = –0.73, Pearson correlation) with the number of expressed genes in our samples. In zebrafish, where there are abundant transcriptional regulators, the number of expressed genes is low. We argue therefore in zebrafish that IR is not as important comparatively as it is in organisms like avian species where IR is a dominant mechanism of post-transcriptional gene expression control. Of note, intra-sample comparisons of gene expression values resulted in no consistent picture, showing lower median expression levels (FPKM values) of intron-retaining genes only in dog, chicken, and zebrafish (Additional file 2: Figure S3).
Despite the number of intron-retaining genes, it is also important to take into account the density of retained introns. Eukaryotic species differ substantially in their intronic density . Some species have less than 100 introns in their total genome but through spliceosomal intron evolution higher developed organisms have, on average, up to eight introns per gene . In our present study humans exhibit the highest density of introns with 9.6 introns per kilobase-pair (kbp) of exonic sequences. However, chicken has the highest density of retained introns (0.53 retained introns/kbp exon), which is almost three times more than the species with the second highest density (dog, 0.18 retained introns/kbp exon; Fig. 1e).
Our observations suggest that IR is a phenomenon, among others, implemented to preserve transcriptomic complexity in genomes with fewer protein-coding genes. Here, complexity is effected by realizing fine-tuning of gene expression control and thereby allowing a cell to adapt to environmental changes . Therefore, we propose that IR enhances gene regulatory complexity in vertebrate species.
Characteristics of retained introns are well-conserved
Others have shown previously that weak splice sites favor IR . Using maximum entropy modeling , we confirmed this characteristic of retained introns in our data and thus demonstrate that this feature is well-conserved in all species investigated (Fig. 2c). The pattern of splice site pair entropies shown in the bivariate histograms suggests that often both splice sites of retained introns are weak, thereby predisposing them for retention.
Retained introns are known to have, on average, a higher GC content compared to non-retained introns [2, 3]. We confirmed this trend in this wider spectrum of species, where the highest GC content was found in retained introns of the mammalian representatives (Fig. 2d). This reinforces our previous observations in human and mouse granulocytes as well as in murine megakaryocytes and erythrocytes [3, 4].
Intron-flanking domains expanded faster during proteomic evolution than other protein domains . These mobile domains have a strong preference for phase 1 introns. Although retained introns also exhibit a phase 0 excess as observed in all introns , our data indicate a slight but consistent shift of the intron phase distribution away from phase 0 in retained introns in all five species (Additional file 2: Figure S6).
We have shown previously that many retained introns harbor PTCs . By comparing the density of PTCs in retained vs non-retained introns, we found that retained introns incorporate slightly but significantly lower PTC densities (Additional file 2: Figure S7). The underrepresentation of PTCs in retained introns might be due to the contribution of intron-retaining isoforms that are not destined for NMD.
We also observed that transcripts with a larger number of introns are more prone to be affected by IR (Fig. 3b). This observation is consistent with previous studies that have identified the increase of the number and length of introns in higher developed organisms as a way to induce genomic complexity [41, 42].
In examining diverse species in a well-defined cell, our study corroborates previously observed characteristics of retained introns (Additional file 2: Table S3). Additionally, we demonstrated that these characteristics are well-conserved. Patterns of IR at the gene level suggest that often several different introns are retained and that transcripts with a larger number of introns have a higher probability of IR events (Additional file 2: Figure S11). These observations can be linked to previous hypotheses that the ratio of non-coding to protein-coding DNA rises as a function of transcriptomic complexity  and that introns fulfill many essential functions, including the regulation of gene expression, in intron-rich species .
Intron retention complements miRNA-mediated gene regulation
Interestingly, in human and mouse many retained introns are located in the 3′ UTR (Additional file 2: Figure S12), i.e. they reside between untranslated exons and are typically spliced out during the mRNA maturation process . This observation suggests the existence of novel yet unannotated 3′ UTR isoforms. Because longer 3′ UTR sequences may harbor additional miRNA binding sites , we wondered whether intron-retaining transcripts might act as competing endogenous RNAs or miRNA sponges [6, 52]. To further investigate this possibility, we compared the 3′ UTR lengths of genes with and without retained introns in their cognate mature transcripts. Surprisingly, we found that the mean 3′ UTR length of intron-retaining genes is significantly longer than that of non-intron-retaining genes in all species (Fig. 5b). The difference increases in species that are evolutionarily closer to human, while in humans the median 3′ UTR sequence of intron retaining genes is more than twice as long compared to the median in non-intron-retaining genes (Fig. 5b). Our evidence suggests that IR preferentially occurs in longer, more complex genes.
We predicted miRNA binding sites in the 3′ UTR sequences of genes with retained introns and all other genes (not incorporating any 3′ UTR intron sequences). Surprisingly, in all species the number of putative miRNA binding sites is drastically increased in genes with retained introns (Fig. 5c). The difference in miRNA binding sites in intron-retaining genes relative to non-intron-retaining genes increases with species that are evolutionarily closer to human (zebrafish: 46%; chicken: 61%; dog: 90%; mouse: 126%; and human: 256% increase). With retained introns in the 3′ UTR, the number of predicted miRNA binding sites increases further by 186.3, 124.1, 18.1, 70.4, and 28.9 additional sites, on average, in human, mouse, dog, chicken, and zebrafish, respectively. This leads us to hypothesize that the expression of genes subject to IR is also controlled by miRNAs. MiRNA target prediction also revealed a significantly higher density of putative miRNA binding sites in sequences of retained introns compared to non-retained introns (Additional file 2: Figure S13). IR transcripts could therefore function as miRNA sponges to indirectly regulate other transcripts by modulating the available pool of miRNAs (Fig. 5e). Six examples in which retained introns may facilitate a miRNA sponge effect were derived from our previous data comparing promyelocyte against granulocyte transcriptomes in mice (Additional file 2: Figure S14; GEO accession numbers: GSE48307 , GSE57624 ). Moreover, we show based on public Ago2 HITS-CLIP data that Argonaute and possibly associated miRNAs can potentially bind to the predicted miRNA binding sites in introns (Additional file 2: Figure S15). We have also illustrated the putative effects of different miRNA expression and intron-retaining transcript levels on the expression of endogenous miRNA targets using a kinetic model based on ordinary differential equations (Additional file 2: Figure S16).
In addition, we identified significantly over-represented sequence motifs (putative miRNA seed sites) in the 3′ UTR sequences of intron-retaining genes using Sylamer . Interestingly, we found strong similarities among the enriched sequence motifs in mammalian species (Fig. 5d; Additional file 2: Figure S17 and Table S4) and the motifs are, on average, more conserved than their flanking regions (Additional file 2: Figure S18). Taken together, these results indicate that IR is a mechanism of post-transcriptional gene regulation that complements miRNA-mediated target repression. However, given that many 3′ UTR introns are retained and that intron-retaining transcripts have more miRNA binding sites, they may also act as miRNA sponges as we previously proposed  (Fig. 5e). In support of this notion is our observation that the enriched sequence motifs are also over-represented in the 3′ UTRs of genes upregulated in hematopoietic differentiation, putatively benefiting a relief from miRNA-induced expression control through intron-retaining miRNA sponge genes (Additional file 2: Figure S20).
Of all forms of alternative splicing, least is known about IR. One reason is that IR events are difficult to detect because cellular surveillance mechanisms like NMD can rapidly degrade transcripts with retained introns, although under certain circumstances primary transcripts can evade NMD by detention in the nucleus [2, 8, 9, 11, 12]. Such differences can be separated experimentally by performing nuclear:cytoplasmic fractionation, as we have previously described . Furthermore, read mapping is challenging as introns are long and abundant in low-complexity regions . Therefore, most transcriptomic studies focusing on alternative splicing have overlooked this type of regulation, despite its potential impact and the multitude of possible downstream effects .
In this in-depth phylogenetic exploration of IR in three mammalian and two non-mammalian vertebrate species, which shared a common ancestral genome 430 million years ago, we have shown that IR provides a conserved and orchestrated mechanism of post-transcriptional gene regulation. Since different subtypes of cells have vastly different mRNA splicing patterns associated with different functions, in this study we investigated IR in a highly purified and functionally defined cell type. By adopting this meticulous approach to benchmarking IR, we have extracted unique insights regarding the conservation and function of IR across species, which would otherwise likely be obscured when performing whole-tissue analysis [2, 3, 57, 58].
A surprising outcome arising from this study was the strong anti-correlation between the number of intron-retaining genes and the number of protein-coding genes in a genome (Fig. 1d). This contrasts with the known increase in intron quantity with genome size, thereby facilitating increased transcriptomic complexity . The false assumption that developmental complexity would be reflected by the number of protein-coding genes in a genome, referred to as the G-value paradox , was partially explained by alternative splicing phenomena that introduce proteomic complexity through novel protein isoforms [22, 29–31, 42]. An alternative measure suggested to account for genomic complexity is the intron density in eukaryotic organisms , which relates to the capacity in realizing alternative splicing events [5, 34]. By studying IR in detail, we have observed how alternative splicing utilizes introns as cis-acting regulatory gene elements to post-transcriptionally fine-tune gene expression. Therefore, we suggest that IR increases gene regulatory complexity and refer to: (1) the increased number of mRNA isoforms detectable due to IR; (2) the increased sophistication in gene expression fine-tuning (possible benefits illustrated in Additional file 2: Figure S19a/b) ; and (3) IR-induced complexity on a molecular network level (i.e. gene regulatory networks, metabolic networks, signaling networks) by introducing dose-dependent non-linear dynamics (Additional file 2: Figure S19b/c) . However, the ultimate function of IR can only be evaluated when the fates of intron-retaining transcripts are determined . Therefore, we cannot rule out other explanations for the observed anti-correlation between the number of intron-retaining genes and the number of protein-coding genes in a genome. For example, the relative absence of IR transcripts in zebrafish granulocytes, which also express a comparatively small number of genes, could either indicate an absence of IR, or it could reflect more efficient degradation of IR transcripts than in chicken with both a high number of expressed genes and high incidence of detected IR.
Another important observation from this analysis is that intron-retaining genes harbor a larger number of miRNA binding sites (Fig. 5c), mainly generated by the presence of longer 3′ UTR sequences than occur in other genes (Fig. 5b). It has been shown that the length of 3′ UTRs is correlated with morphological complexity in metazoan species  and that gene regulation by multiple and cooperating miRNAs mediates enhanced target repression [63–65]. This indicates that IR-mediated decay and miRNA-induced translational repression may be complementary mechanisms orchestrating post-transcriptional gene expression control.
Our analysis reveals that IR does not just affect many gene orthologs but also encompasses other functionally related genes, suggesting that IR is a function-centric form of gene regulation. Many of the intron-retaining genes are downregulated in differentiated granulocytes. We found, for example, 674 human intron-retaining genes to be more than twofold downregulated in the study of Theilgaard-Mönch et al., in which the authors compare gene expression profiles of human promyelocytes, myelocytes, and neutrophils . The authors describe a general decline of proliferative and general cellular activity during terminal granulocytic differentiation. Here, IR seems to be a crucial regulatory factor. Downregulated genes that show IR are enriched in acetylation-related genes and splicing factors, as well as phosphoproteins and kinases and thus affect general gene expression and activity on the transcriptional, post-transcriptional, and post-translational levels, respectively (Additional file 2: Figure S21). This may also explain the G0/G1 arrest and downregulation of kinase expression observed in most end-stage differentiated neutrophil granulocytes in the study by Klausen et al. .
The exact mechanisms leading to IR remain to be elucidated; however, it is known that certain features increase the likelihood of an intron to be retained [36, 67]. These features are mainly composed of cis-regulatory elements marking characteristics of retained introns and their host genes [68, 69]. In our phylogenetic study of IR in five species we confirmed conservation of such features including weaker splice sites, a higher GC content, and a shorter length of retained introns. Furthermore, we found that retained introns are predominantly located near the 3′ termini of transcripts and have lower PTC densities than non-retained introns.
Most of the observations that we found conserved in all species are particularly pronounced in the avian representative. Chicken granulocytes contained the most intron-retaining genes both in number and in relation to the total number of expressed genes (~40%). Chicken also has the highest density of retained introns; however, their median length is larger than that observed in the other species (Fig. 2a). This is surprising because intron size correlates with genome size and bird introns are typically shorter than that of mammals . This apparent paradox may have two explanations: (1) retained introns in chicken have other functions beyond regulating the expression of their host genes; or (2) they may contain more cis-regulatory elements that interact with splicing factors. Moreover, many of the chicken genes are presumably co-regulated (15% of genes and 20% of the intron-retaining genes share a mutual promoter region) and thus are more dependent on post-transcriptional gene regulation (Fig. 4, Additional file 2: Figure S10). This observation contradicts an earlier statement made by Koyanag et al. who studied the evolution of bidirectional gene pairs that share a mutual promoter region and found that enrichment in bidirectional gene pairs is only detectable in mammals and not in other eukaryotes .
However, the variation in IR abundance may also be explained by cell biological nuances in each species. Chicken heterophils for example are exceptional in not having a myeloperoxidase-like activity whereas this is the most abundant protein in neutrophils of the other species . In general, IR calling is influenced by sequencing depth and annotation quality, i.e. the more reads and annotated genes/introns the more IR events should be detected. However, this has a negligible impact on our analysis, as the sequencing depth is comparable in all experiments. The fact that the number of retained introns detected in chicken exceeds that of all other species (including human, with the highest number of annotated introns) suggests that our results and conclusions are not biased by annotation quality.
In summary, our study has provided a definitive documentation of the conserved characteristics exhibited by IR in vertebrate granulocytes, including humans. We have provided new insights that support the notion of IR as an independent mechanism of gene regulation that may interfere with or complement other forms of post-transcriptional gene regulation. In IR we see a form of alternative splicing that realizes a feature contributing to gene regulatory complexity thereby facilitating organismal propensity for adaptation.
A whole blood sample was obtained from a healthy male individual. Peripheral blood leukocytes were separated from red cells and platelets using dextran sedimentation and Ficoll density separation. Human granulocytes (CD11b + CD15+) were isolated using fluorescence-activated cell sorting (FACS) as previously described .
Peripheral blood from ten male dogs (Beagles aged 1–8 years) were collected from Novartis Animal Health. Dog granulocytes were purified using Percoll density separation as previously described , followed by FACS using a monoclonal antibody against canine neutrophils (CAD048A, Monoclonal Antibody Center, Washington State University). Purity of granulocytes was > 95% based on morphological assessment.
Chicken granulocytes were isolated from peripheral blood of six male Ross breed chickens (Gallus gallus domesticus) aged eight weeks. Following blood collection, heterophil-granulocytes were isolated as previously described . Briefly, whole blood was diluted 1:1 with RPMI 1640 media (Sigma) containing 1% methylcellulose (25 centipoises; Sigma) and centrifuged (25 g, 30 min at 4 °C). The supernatant was transferred to a new tube, washed with calcium and magnesium-free Hank’s balanced salt solution (HBSS, 1:1; Sigma), and layered onto discontinuous Ficoll-Histopaque (Sigma) gradient (specific gravity 1.077 over specific gravity 1.119). The gradients were centrifuged at 250 g for 60 min at 4 °C. After centrifugation, the Histopaque layer containing the granulocytes was collected at the second interface 1.077/1.119 and transferred to a new Falcon tube. Cells were washed three times in RPMI 1640 media and pelleted by centrifugation at 4 °C, 10 min, 200 g. The last cell pellet was resuspended in calcium and magnesium-free Hank’s balanced salt solution with fetal bovine serum. Cells were further purified based on low forward and high side scatter using FACS to achieve a purity of > 95% based on morphological assessment.
Kidneys from zebrafish aged 3–6 months were dissected as previously described , pooled in HBSS (Sigma), homogenized, pelleted by centrifugation (250 g, 15 min), and gently resuspended in 6 mL HBSS. The suspension was gently layered on 2 mL of lymphocyte separation medium 1078 (Mediatech; CellGro, AK, USA) in a 15-mL Falcon tube and centrifuged (400 g, 30 min). The resulting layer of leukocytes was removed with a 1-mL sterile pipette and transferred to a 15-mL tube. HBSS was added to a total volume of 4 mL and leukocytes collected by centrifugation (400 g, 15 min). Yield assessed by hemocytometer cell counts was 1.1 ± 0.6 × 106 cells/kidney (n = 15 independent preparations) and samples were 88.7 ± 6.2% (mean ± SD) granulocytes (n = 9 random fields).
Morphological confirmation of granulocytes was performed following Giemsa (human, mouse, dog, and zebrafish) or Wright staining (chicken) of cells smeared or spun onto poly-L-lysine slides.
RNA isolation and mRNA-seq
Total RNA was isolated from granulocytes using Trizol (Invitrogen). The RNA quality was assessed using RNA 6000 Nano Chips on an Agilent Bioanalyzer (Agilent Technologies) to confirm an RNA integrity score of > 7.0. mRNA-seq was performed by Macrogen (Korea) using the Illumina Hi-Seq 2000 platform. RNA-seq libraries were prepared from > 1 μg of total RNA using TruSeq RNA sample prep kit (Illumina) according to the manufacturers’ instructions.
Genome/gene sequences and gene structure annotations
Whole-genome assemblies of human, mouse, dog, chicken, and zebrafish (GRCh37.75, GRCm38.78, CanFam3.1.78, Galgal4.78, Zv9.78) were downloaded from Ensembl (Release 75). Intron sequences and gene structure information were retrieved from the UCSC Table browser . Data on exon phases were retrieved via the ENSEMBL BioMart interface .
RNA-seq data analysis and identification of IR events
Reference genome files were built and mRNA-seq reads were mapped to the respective reference genomes using STAR (Version 2.4) . Details on sequencing depth and read mapping statistics for each sample are provided in Additional file 2: Table S5 and Figure S22. Gene body coverage was determined using the geneBody coverage module from the RSeQC package (v2.6.3) .
We used the IRFinder algorithm  for the detection of IR events in all known introns. IRFinder estimates the abundance of IR by computing the ratio between gene transcripts retaining an intron and the sum of all transcripts of the respective gene. We refer to this measure as the “IR ratio,” while others have also used the term “percent IR” (PIR) . The IR ratio is in the range of 0–1; however, we only considered introns with an IR ratio ≥ 0.1. We excluded introns with insufficient splicing depth (<4 reads correctly crossing the splice junction) and insufficient coverage (splicing depth + trimmed mean intron depth < 10). IRFinder has a built-in routine to handle confounding factors. For example, partial IR resulting from splicing inside the intron is a distinct process and was not considered. On a per-gene basis, we considered the highest observed IR ratio for any of the retained introns as the gene’s IR ratio.
Gene expression estimation
Gene expression levels specified as FPKM were determined using Cufflinks . FPKM values for the genes in all species are specified in Additional file 4. Ratios of intron-retaining genes to all expressed genes were determined for all genes with an FPKM value ≥ 1.
Annotation enrichment analysis
Gene annotation enrichment was performed using the Database for Annotation, Visualization and Integrated Discovery (DAVID 6.7) . In order to make the analyses comparable across species, we used the set of orthologous genes shared among all species as background. Enriched terms with a p value < 0.05 were considered significant. Most commonly enriched terms are SwissProt and Protein Information Resource keywords (SP_PIR_KEYWORDS). All enriched functional annotations for each cluster of intron-retaining orthologs illustrated in Additional file 2: Figure S1 can be found in Additional file 3. The Circos plot in Fig. 1c was generated using the R package GOplot . We have additionally performed a functional enrichment analysis of all intron-retaining genes using expressed genes as background in each species. The results are attached in Additional file 3.
Orthologs of intron-retaining genes were determined based on orthology relationships extracted from the Ensembl BioMart interface. Venn diagrams were drawn with the VennDiagram R package.
miRNA target site analysis
miRNA sequences and genomic coordinates were downloaded from the miRBase database (release 21) . miRNA target site predictions were performed using miRanda v3.3a  with the energy threshold set to –14 kcal/mol and requiring strict alignment in the seed region (offset positions 2–8). Seed site enrichment analysis was performed using Sylamer , which calculates cumulative hypergeometric p values associated with small word occurrences in a sequence repository. 3′ UTR sequences of intron retaining genes were tested for 6mer seed site enrichment using the 3′ UTR sequences of all genes as background.
Splice-site strength analysis
We used the maximum entropy model prosed in Yeo and Burge  to estimate the strengths of donor and acceptor sites in retained and non-retained introns.
3′ UTR introns
GTF files for all species under investigation were retrieved from the ENSEMBL ftp server (ftp.ensembl.org). Introns were flagged as 3′ UTR-based when located in the 3′ UTR of any of the transcripts of a gene.
All statistical tests were performed using the statistical programming language R and are specified in the main text. In Fig. 2b, we used the geom_smooth function of the R ggplot2 package, which uses a generalized additive model with integrated smoothness estimation to fit a curve and standard error bounds to the intron length to IR ratio relationship.
IR profiles of orthologous genes in Additional file 2: Figure S1 were quantile normalized and grouped based on k-means clustering (with Euclidian distance). Species-based profiles (columns) were grouped using a hierarchical clustering approach (1-pearson average linkage). Clustering and heat map visualizations were performed using GENE-E (http://www.broadinstitute.org/cancer/software/GENE-E/).
We thank Kim Baker and Elanco (formerly Novartis Animal Health) for providing the peripheral blood from dogs. We thank Steven Allen, Frank Kao, and Suat Dervish for FACS. The authors acknowledge the Sydney Informatics Hub and the University of Sydney’s high-performance computing cluster, Artemis, for providing the high-performance computing resources that have contributed to the research results reported within this paper.
This work was supported by the National Health and Medical Research Council (Grant #1061906 to JEJR and WR, #1080530 to JEJR, WR, and JJW, #1044754 and #1086020 to GJL, #1126306 to JJW, JEJR), Cure the Future (JEJR and US), and an anonymous foundation (JEJR). JJW and WR hold Fellowships from the Cancer Institute of NSW. US was supported by the Sydney Research Excellence Initiative. SA was supported by the King Khalid University, ABHA, KSA and the Saudi Arabian Cultural Attache in Australia. The Australian Regenerative Medicine Institute is supported by funds from the State Government of Victoria and the Australian Government.
Availability of data and materials
All RNA-seq data are available at the NCBI Sequence Read Archive (SRA project accession number: SRP092128 ). All other data generated during this study are included in this published article and its supplementary information files.
JEJR conceived the overall project, and supervised its execution. US, JJW, and JEJR designed the experiments. US, WR, and FJ performed statistical and bioinformatics analyses. NP, SA, SS, MCK, GJL, and JJW purified granulocytes from human, mice, dogs, chicken, and zebrafish and prepared RNA for RNA-seq. US, JJW, and JEJR wrote and revised the manuscript. All authors reviewed the results and approved the manuscript.
Ethics approval and consent to participate
The human whole blood sample was obtained with ethics approval from the Human Research Ethics Committee of the Royal Prince Alfred Hospital (HREC/08/RPAH/222). The human participant has given written informed consent. Experimental methods comply with the Helsinki Declaration. Ethics approval for the primary mouse granulocytes from bone marrow of male C57BL/6J mice (8–10 weeks) was obtained from RPAH AEC Protocol no. 2013/045. Dog peripheral blood samples were collected from Novartis Animal Health with ethics approval (Protocol no. YAR-13-001 and RPAH AEC Protocol no. 2013/045). Chicken peripheral blood was collected with ethics approval (UQ AEC no. SVS/365/13/ANNANDALE and RPAH AEC Protocol no. 2013/045). The zebrafish experiments were approved by the Monash University Animal Ethics Committee (protocol MAS-2010-18).
Consent for publication
The authors declare that they have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Barash Y, Calarco JA, Gao W, Pan Q, Wang X, Shai O, Blencowe BJ, Frey BJ. Deciphering the splicing code. Nature. 2010;465:53–9.View ArticlePubMedGoogle Scholar
- Braunschweig U, Barbosa-Morais NL, Pan Q, Nachman EN, Alipanahi B, Gonatopoulos-Pournatzis T, et al. Widespread intron retention in mammals functionally tunes transcriptomes. Genome Res. 2014;24:1774–86.View ArticlePubMedPubMed CentralGoogle Scholar
- Wong JJ, Ritchie W, Ebner OA, Selbach M, Wong JW, Huang Y, et al. Orchestrated intron retention regulates normal granulocyte differentiation. Cell. 2013;154:583–95.View ArticlePubMedGoogle Scholar
- Edwards CR, Ritchie W, Wong JJ, Schmitz U, Middleton R, An X, et al. A dynamic intron retention program in the mammalian megakaryocyte and erythrocyte lineages. Blood. 2016;127:e24–34.View ArticlePubMed CentralGoogle Scholar
- Ni T, Yang W, Han M, Zhang Y, Shen T, Nie H, et al. Global intron retention mediated gene regulation during CD4+ T cell activation. Nucleic Acids Res. 2016;44:6817–29.View ArticlePubMedPubMed CentralGoogle Scholar
- Wong JJ, Au AY, Ritchie W, Rasko JE. Intron retention in mRNA: No longer nonsense: Known and putative roles of intron retention in normal and disease biology. Bioessays. 2015;38:41–9.View ArticlePubMedGoogle Scholar
- Kurosaki T, Maquat LE. Nonsense-mediated mRNA decay in humans at a glance. J Cell Sci. 2016;129:461–7.View ArticlePubMedPubMed CentralGoogle Scholar
- Boutz PL, Bhutkar A, Sharp PA. Detained introns are a novel, widespread class of post-transcriptionally spliced introns. Genes Dev. 2015;29:63–80.View ArticlePubMedPubMed CentralGoogle Scholar
- Pirnie SP, Osman A, Zhu Y, Carmichael GG. An Ultraconserved Element (UCE) controls homeostatic splicing of ARGLU1 mRNA. Nucleic Acids Res. 2016;45:3473–86.View ArticlePubMed CentralGoogle Scholar
- Mauger O, Lemoine F, Scheiffele P. Targeted intron retention and excision for rapid gene regulation in response to neuronal activity. Neuron. 2016;92:1266–78.View ArticlePubMedGoogle Scholar
- Pimentel H, Parra M, Gee SL, Mohandas N, Pachter L, Conboy JG. A dynamic intron retention program enriched in RNA processing genes regulates gene expression during terminal erythropoiesis. Nucleic Acids Res. 2016;44:838–51.View ArticlePubMedGoogle Scholar
- Naro C, Jolly A, Di Persio S, Bielli P, Setterblad N, Alberdi AJ, et al. An orchestrated intron retention program in meiosis controls timely usage of transcripts during germ cell differentiation. Dev Cell. 2017;41:82–93.View ArticlePubMedPubMed CentralGoogle Scholar
- Jacob AG, Smith CW. Intron retention as a component of regulated gene expression programs. Hum Genet. 2017. doi: 10.1007/s00439-017-1791-xGoogle Scholar
- Boothby TC, Zipper RS, van der Weele CM, Wolniak SM. Removal of retained introns regulates translation in the rapidly developing gametophyte of Marsilea vestita. Dev Cell. 2013;24:517–29.View ArticlePubMedGoogle Scholar
- Bell TJ, Miyashiro KY, Sul JY, Buckley PT, Lee MT, McCullough R, et al. Intron retention facilitates splice variant diversity in calcium-activated big potassium channel populations. Proc Natl Acad Sci U S A. 2010;107:21152–7.View ArticlePubMedPubMed CentralGoogle Scholar
- Cuenca-Bono B, Garcia-Molinero V, Pascual-Garcia P, Dopazo H, Llopis A, Vilardell J, et al. SUS1 introns are required for efficient mRNA nuclear export in yeast. Nucleic Acids Res. 2011;39:8599–611.View ArticlePubMedPubMed CentralGoogle Scholar
- Gontijo AM, Miguela V, Whiting MF, Woodruff RC, Dominguez M. Intron retention in the Drosophila melanogaster Rieske Iron Sulphur Protein gene generated a new protein. Nat Commun. 2011;2:323.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhang Q, Li H, Jin H, Tan H, Zhang J, Sheng S. The global landscape of intron retentions in lung adenocarcinoma. BMC Med Genomics. 2014;7:15.View ArticlePubMedPubMed CentralGoogle Scholar
- Jung H, Lee D, Lee J, Park D, Kim YJ, Park WY, et al. Intron retention is a widespread mechanism of tumor-suppressor inactivation. Nat Genet. 2015;47:1242–8.View ArticlePubMedGoogle Scholar
- Buckley PT, Lee MT, Sul JY, Miyashiro KY, Bell TJ, Fisher SA, et al. Cytoplasmic intron sequence-retaining transcripts can be dendritically targeted via ID element retrotransposons. Neuron. 2011;69:877–84.View ArticlePubMedPubMed CentralGoogle Scholar
- Dvinge H, Bradley RK. Widespread intron retention diversifies most cancer transcriptomes. Genome Med. 2015;7:45.View ArticlePubMedPubMed CentralGoogle Scholar
- Chen L, Bush SJ, Tovar-Corona JM, Castillo-Morales A, Urrutia AO. Correcting for differential transcript coverage reveals a strong relationship between alternative splicing and organism complexity. Mol Biol Evol. 2014;31:1402–13.View ArticlePubMedPubMed CentralGoogle Scholar
- Barbosa-Morais NL, Irimia M, Pan Q, Xiong HY, Gueroussov S, Lee LJ, et al. The evolutionary landscape of alternative splicing in vertebrate species. Science. 2012;338:1587–93.View ArticlePubMedGoogle Scholar
- Amores A, Catchen J, Ferrara A, Fontenot Q, Postlethwait JH. Genome evolution and meiotic maps by massively parallel DNA sequencing: spotted gar, an outgroup for the teleost genome duplication. Genetics. 2011;188:799–808.View ArticlePubMedPubMed CentralGoogle Scholar
- Havixbeck JJ, Barreda DR. Neutrophil development, migration, and function in teleost fish. Biology. 2015;4:715–34.View ArticlePubMedPubMed CentralGoogle Scholar
- Middleton R, Gao D, Thomas A, Singh B, Au A, Wong JJ-L, et al. IRFinder: assessing the impact of intron retention on mammalian gene expression. Genome Biol. 2017;18:51.View ArticlePubMedPubMed CentralGoogle Scholar
- Theilgaard-Monch K, Jacobsen LC, Borup R, Rasmussen T, Bjerregaard MD, Nielsen FC, et al. The transcriptional program of terminal granulocytic differentiation. Blood. 2005;105:1785–96.View ArticlePubMedGoogle Scholar
- Jaillon O, Bouhouche K, Gout JF, Aury JM, Noel B, Saudemont B, et al. Translational control of intron splicing in eukaryotes. Nature. 2008;451:359–62.View ArticlePubMedGoogle Scholar
- Blencowe BJ. Alternative splicing: new insights from global analyses. Cell. 2006;126:37–47.View ArticlePubMedGoogle Scholar
- Black DL. Protein diversity from alternative splicing: a challenge for bioinformatics and post-genome biology. Cell. 2000;103:367–70.View ArticlePubMedGoogle Scholar
- Kriventseva EV, Koch I, Apweiler R, Vingron M, Bork P, Gelfand MS, et al. Increase of functional diversity by alternative splicing. Trends Genet. 2003;19:124–8.View ArticlePubMedGoogle Scholar
- Resch A, Xing Y, Modrek B, Gorlick M, Riley R, Lee C. Assessing the impact of alternative splicing on domain interactions in the human proteome. J Proteome Res. 2004;3:76–83.View ArticlePubMedGoogle Scholar
- Zhang HM, Chen H, Liu W, Liu H, Gong J, Wang H, et al. AnimalTFDB: a comprehensive animal transcription factor database. Nucleic Acids Res. 2012;40:D144–149.View ArticlePubMedGoogle Scholar
- Rogozin IB, Carmel L, Csuros M, Koonin EV. Origin and evolution of spliceosomal introns. Biol Direct. 2012;7:11.View ArticlePubMedPubMed CentralGoogle Scholar
- Roy SW, Gilbert W. The evolution of spliceosomal introns: patterns, puzzles and progress. Nat Rev Genet. 2006;7:211–21.PubMedGoogle Scholar
- Ge Y, Porse BT. The functional consequences of intron retention: alternative splicing coupled to NMD as a regulator of gene expression. Bioessays. 2014;36:236–43.View ArticlePubMedGoogle Scholar
- Yeo G, Burge CB. Maximum entropy modeling of short sequence motifs with applications to RNA splicing signals. J Comput Biol. 2004;11:377–94.View ArticlePubMedGoogle Scholar
- Liu M, Walch H, Wu S, Grigoriev A. Significant expansion of exon-bordering protein domains during animal proteome evolution. Nucleic Acids Res. 2005;33:95–105.View ArticlePubMedPubMed CentralGoogle Scholar
- Nguyen HD, Yoshihama M, Kenmochi N. Phase distribution of spliceosomal introns: implications for intron origin. BMC Evol Biol. 2006;6:69.View ArticlePubMedPubMed CentralGoogle Scholar
- Lykke-Andersen S, Jensen TH. Nonsense-mediated mRNA decay: an intricate machinery that shapes transcriptomes. Nat Rev Mol Cell Biol. 2015;16:665–77.View ArticlePubMedGoogle Scholar
- Lynch M, Conery JS. The origins of genome complexity. Science. 2003;302:1401–4.View ArticlePubMedGoogle Scholar
- Taft RJ, Pheasant M, Mattick JS. The relationship between non-protein-coding DNA and eukaryotic complexity. Bioessays. 2007;29:288–99.View ArticlePubMedGoogle Scholar
- Adachi N, Lieber MR. Bidirectional gene organization: a common architectural feature of the human genome. Cell. 2002;109:807–9.View ArticlePubMedGoogle Scholar
- Takai D, Jones PA. Origins of bidirectional promoters: computational analyses of intergenic distance in the human genome. Mol Biol Evol. 2004;21:463–7.View ArticlePubMedGoogle Scholar
- Trinklein ND, Aldred SF, Hartman SJ, Schroeder DI, Otillar RP, Myers RM. An abundance of bidirectional promoters in the human genome. Genome Res. 2004;14:62–6.View ArticlePubMedPubMed CentralGoogle Scholar
- Koyanagi KO, Hagiwara M, Itoh T, Gojobori T, Imanishi T. Comparative genomics of bidirectional gene pairs and its implications for the evolution of a transcriptional regulation system. Gene. 2005;353:169–76.View ArticlePubMedGoogle Scholar
- Mattick JS. RNA regulation: a new genetics? Nat Rev Genet. 2004;5:316–23.View ArticlePubMedGoogle Scholar
- Chorev M, Carmel L. The function of introns. Front Genet. 2012;3:55.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhang Q, Edwards SV. The evolution of intron size in amniotes: a role for powered flight? Genome Biol Evol. 2012;4:1033–43.View ArticlePubMedPubMed CentralGoogle Scholar
- Bicknell AA, Cenik C, Chua HN, Roth FP, Moore MJ. Introns in UTRs: why we should stop ignoring them. Bioessays. 2012;34:1025–34.View ArticlePubMedGoogle Scholar
- Tan S, Guo J, Huang Q, Chen X, Li-Ling J, Li Q, et al. Retained introns increase putative microRNA targets within 3′ UTRs of human mRNA. FEBS Lett. 2007;581:1081–6.View ArticlePubMedGoogle Scholar
- Salmena L, Poliseno L, Tay Y, Kats L, Pandolfi PP. A ceRNA hypothesis: the Rosetta Stone of a hidden RNA language? Cell. 2011;146:353–8.View ArticlePubMedPubMed CentralGoogle Scholar
- Wong JJ, Ritchie W, Ebner OA, Selbach M, Wong JW, Huang Y, et al. Orchestrated intron retention regulates normal granulocyte differentiation. Gene Expression Omnibus. 2013. https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE48307. Accessed 18 July 2016.
- Wong JJ, Ritchie W, Gao D, Lau KA, Gonzalez M, Choudhary A, et al. Identification of nuclear-enriched miRNAs during mouse granulopoiesis. Gene Expression Omnibus. 2014. https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE57624. Accessed 1 Mar 2017.
- van Dongen S, Abreu-Goodger C, Enright AJ. Detecting microRNA binding and siRNA off-target effects from expression data. Nat Methods. 2008;5:1023–5.View ArticlePubMedPubMed CentralGoogle Scholar
- Vanichkina DP, Schmitz U, Wong JJ, Rasko JEJ. Challenges in defining the role of intron retention in normal biology and disease. Semin Cell Dev Biol. 2017. 10.1016/j.semcdb.2017.07.030Google Scholar
- Yap K, Lim ZQ, Khandelia P, Friedman B, Makeyev EV. Coordinated regulation of neuronal mRNA steady-state levels through developmentally controlled intron retention. Genes Dev. 2012;26:1209–23.View ArticlePubMedPubMed CentralGoogle Scholar
- Welch JD, Williams LA, DiSalvo M, Brandt AT, Marayati R, Sims CE, et al. Selective single cell isolation for genomics using microraft arrays. Nucleic Acids Res. 2016;44:8292–301.View ArticlePubMedPubMed CentralGoogle Scholar
- Hahn MW, Wray GA. The g-value paradox. Evol Dev. 2002;4:73–5.View ArticlePubMedGoogle Scholar
- Brawand D, Soumillon M, Necsulea A, Julien P, Csardi G, Harrigan P, et al. The evolution of gene expression levels in mammalian organs. Nature. 2011;478:343–8.View ArticlePubMedGoogle Scholar
- Tyson JJ, Chen KC, Novak B. Sniffers, buzzers, toggles and blinkers: dynamics of regulatory and signaling pathways in the cell. Curr Opin Cell Biol. 2003;15:221–31.View ArticlePubMedGoogle Scholar
- Chen CY, Chen ST, Juan HF, Huang HC. Lengthening of 3′UTR increases with morphological complexity in animal evolution. Bioinformatics. 2012;28:3178–81.View ArticlePubMedGoogle Scholar
- Schmitz U, Lai X, Winter F, Wolkenhauer O, Vera J, Gupta SK. Cooperative gene regulation by microRNA pairs and their identification using a computational workflow. Nucleic Acids Res. 2014;42:7539–52.View ArticlePubMedPubMed CentralGoogle Scholar
- Lai X, Bhattacharya A, Schmitz U, Kunz M, Vera J, Wolkenhauer O. A systems’ biology approach to study microRNA-mediated gene regulatory networks. Biomed Res Int. 2013;2013:703849.PubMedPubMed CentralGoogle Scholar
- Lai X, Schmitz U, Gupta SK, Bhattacharya A, Kunz M, Wolkenhauer O, et al. Computational analysis of target hub gene repression regulated by multiple and cooperative miRNAs. Nucleic Acids Res. 2012;40:8818–34.View ArticlePubMedPubMed CentralGoogle Scholar
- Klausen P, Bjerregaard MD, Borregaard N, Cowland JB. End-stage differentiation of neutrophil granulocytes in vivo is accompanied by up-regulation of p27kip1 and down-regulation of CDK2, CDK4, and CDK6. J Leukoc Biol. 2004;75:569–78.View ArticlePubMedGoogle Scholar
- Wong JJ, Gao D, Nguyen TV, Kwok CT, van Geldermalsen M, Middleton R, et al. Intron retention is regulated by altered MeCP2-mediated splicing factor recruitment. Nat Commun. 2017;8:15134.View ArticlePubMedPubMed CentralGoogle Scholar
- Sakabe NJ, de Souza SJ. Sequence features responsible for intron retention in human. BMC Genomics. 2007;8:59.View ArticlePubMedPubMed CentralGoogle Scholar
- Wang Z, Burge CB. Splicing regulation: from a parts list of regulatory elements to an integrated splicing code. RNA. 2008;14:802–13.View ArticlePubMedPubMed CentralGoogle Scholar
- Genovese KJ, He H, Swaggerty CL, Kogut MH. The avian heterophil. Dev Comp Immunol. 2013;41:334–40.View ArticlePubMedGoogle Scholar
- Wong JJ, Ritchie W, Gao D, Lau KA, Gonzalez M, Choudhary A, et al. Identification of nuclear-enriched miRNAs during mouse granulopoiesis. J Hematol Oncol. 2014;7:42.View ArticlePubMedPubMed CentralGoogle Scholar
- Weiss DJ, Kraemer R, Schmit K. Isolation of granulocytes and mononuclear cells from the blood of dogs, cats, horses and cattle. Vet Clin Pathol. 1989;18:33–6.View ArticlePubMedGoogle Scholar
- Shini S, Shini A, Kaiser P. Cytokine and chemokine gene expression profiles in heterophils from chickens treated with corticosterone. Stress. 2010;13:185–94.View ArticlePubMedGoogle Scholar
- Lieschke GJ, Oates AC, Crowhurst MO, Ward AC, Layton JE. Morphologic and functional characterization of granulocytes and macrophages in embryonic and adult zebrafish. Blood. 2001;98:3087–96.View ArticlePubMedGoogle Scholar
- Karolchik D, Hinrichs AS, Furey TS, Roskin KM, Sugnet CW, Haussler D, et al. The UCSC Table Browser data retrieval tool. Nucleic Acids Res. 2004;32:D493–496.View ArticlePubMedPubMed CentralGoogle Scholar
- Flicek P, Amode MR, Barrell D, Beal K, Billis K, Brent S, et al. Ensembl 2014. Nucleic Acids Res. 2014;42:D749–755.View ArticlePubMedGoogle Scholar
- Dobin A, Davis CA, Schlesinger F, Drenkow J, Zaleski C, Jha S, et al. STAR: ultrafast universal RNA-seq aligner. Bioinformatics. 2013;29:15–21.View ArticlePubMedGoogle Scholar
- Wang L, Wang S, Li W. RSeQC: quality control of RNA-seq experiments. Bioinformatics. 2012;28:2184–5.View ArticlePubMedGoogle Scholar
- Trapnell C, Williams BA, Pertea G, Mortazavi A, Kwan G, van Baren MJ, et al. Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation. Nat Biotechnol. 2010;28:511–5.View ArticlePubMedPubMed CentralGoogle Scholar
- da Huang W, Sherman BT, Lempicki RA. Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc. 2009;4:44–57.View ArticleGoogle Scholar
- Walter W, Sanchez-Cabo F, Ricote M. GOplot: an R package for visually combining expression data with functional analysis. Bioinformatics. 2015;31:2912–4.View ArticlePubMedGoogle Scholar
- Kozomara A, Griffiths-Jones S. miRBase: annotating high confidence microRNAs using deep sequencing data. Nucleic Acids Res. 2014;42:D68–73.View ArticlePubMedGoogle Scholar
- Enright AJ, John B, Gaul U, Tuschl T, Sander C, Marks DS. MicroRNA targets in Drosophila. Genome Biol. 2003;5:R1.View ArticlePubMedPubMed CentralGoogle Scholar
- Schmitz U, Pinello N, Jia F, Alasmari S, Ritchie W, Keightley M-C, et al. Intron retention enhances gene regulatory complexity in vertebrates. NCBI Sequence Read Archive. 2017. https://www.ncbi.nlm.nih.gov/sra/?term=SRP092128.Google Scholar