- Open Access
Topological organization and dynamic regulation of human tRNA genes during macrophage differentiation
© The Author(s). 2017
- Received: 10 April 2017
- Accepted: 25 August 2017
- Published: 20 September 2017
The human genome is hierarchically organized into local and long-range structures that help shape cell-type-specific transcription patterns. Transfer RNA (tRNA) genes (tDNAs), which are transcribed by RNA polymerase III (RNAPIII) and encode RNA molecules responsible for translation, are dispersed throughout the genome and, in many cases, linearly organized into genomic clusters with other tDNAs. Whether the location and three-dimensional organization of tDNAs contribute to the activity of these genes has remained difficult to address, due in part to unique challenges related to tRNA sequencing. We therefore devised integrated tDNA expression profiling, a method that combines RNAPIII mapping with biotin-capture of nascent tRNAs. We apply this method to the study of dynamic tRNA gene regulation during macrophage development and further integrate these data with high-resolution maps of 3D chromatin structure.
Integrated tDNA expression profiling reveals domain-level and loop-based organization of tRNA gene transcription during cellular differentiation. tRNA genes connected by DNA loops, which are proximal to CTCF binding sites and expressed at elevated levels compared to non-loop tDNAs, change coordinately with tDNAs and protein-coding genes at distal ends of interactions mapped by in situ Hi-C. We find that downregulated tRNA genes are specifically marked by enhanced promoter-proximal binding of MAF1, a transcriptional repressor of RNAPIII activity, altogether revealing multiple levels of tDNA regulation during cellular differentiation.
We present evidence of both local and coordinated long-range regulation of human tDNA expression, suggesting the location and organization of tRNA genes contribute to dynamic tDNA activity during macrophage development.
- Topologically associating domains
The role of transfer RNAs (tRNAs) in deciphering the genetic code is universal to cell biology. The trinucleotide anticodon sequence of each tRNA-type decodes specific codons employed by messenger RNAs (mRNAs). Overall, the number of genes encoding each tRNA-type and the relative cellular abundance of each tRNA-type have been shown to correlate with the frequency of codon usage in species-specific and tissue-specific contexts, respectively [1–3]. In eukaryotes, changes in tRNA abundance have been reported across proliferative and senescent cell types and in response to specific perturbations, such as exposure to oxidation and alkylation-related stress [3–5]. Several important extra-translational functions for tRNA and tRNA-derived fragments have also become apparent, such as interfering with transposon reactivation and antagonizing the stability of oncogenic transcripts in breast cancer cells [6–8]. Thus, adjusting the level of cellular tRNA molecules, through both transcriptional and post-transcriptional mechanisms, may be important for modulating translation and potential ancillary activities.
Deciphering the mechanisms by which nascent tRNA levels are dynamically regulated, however, remains difficult to address, due in part to the unique challenges related to tRNA sequencing and alignment, as well as the unique complexity of tRNA biology . In regard to sequencing, cellular tRNAs are heavily modified and consequently difficult to reverse transcribe during library preparation. In recent years, studies have tackled specific challenges associated with tRNA sequencing, or utilized alternate means, such as mapping RNA polymerase III as readout for tDNA expression [10–12]. The use of a dealkylating enzyme, ALKB, improves the fraction of full-length tRNA reads by demethylating sites that block reverse transcription [13–16]. However, sequencing of cellular tRNA levels alone provides little information about the transcriptional activity of tRNA genes, as nascent tRNAs undergo a complex maturation process . Mapping of RNA polymerase III, meanwhile, represents an imperfect measure of tRNA gene activity that does not directly assay the level of nascently transcribed RNA. To this end, biotin-capture based genomic run-on experiments, such as BioGRO and precision nuclear run-on sequencing (PRO-seq), allow quantitative transcriptional profiling and mapping of RNA polymerases [18–21]. Thus, leveraging both RNA polymerase III occupancy with biotin-capture of nascent, demethylated tRNAs may provide a more accurate measure of tRNA gene expression in growing cells.
Interaction-based studies profiling the structure of eukaryotic chromosomes have identified highly self-interacting topological domains, a unit of three-dimensional (3D) organization that divides the genome into local neighborhoods of similar gene activity and restricts the ability of enhancers to influence non-target genes [22–29]. Recent studies mapping global interaction frequencies by in situ high-throughput chromosome conformation capture (in situ Hi-C) have further improved the resolution of physical domain identification and suggest that these contact domains are largely stable across cell types [30–32]. These short-range structures are often established within loops connected by inward oriented CTCF binding sites, an architectural protein originally described by its ability to function as an insulator, and by the cohesin complex and factors that control its association with DNA [30, 33–40]. tRNA genes, which are also enriched at the boundaries of topological domains and, in certain contexts, have been shown to function as insulator elements in the classical sense, have also been reported to play a role in the organization of eukaryotic chromosomes [28, 41–44]. However, to what degree tRNA genes are involved in long-range interactions in humans, and whether the 3D organization of tRNA genes contributes to the activity of these genes themselves remains unknown.
We have recently profiled the 3D organization and long-range interactome of human THP-1 monocytes and THP-1-derived macrophages through deeply sequenced in situ Hi-C experiments. High-resolution mapping of DNA loops identified both static and dynamic loop-based regulation of key macrophage genes during cellular differentiation . THP-1 monocytes were differentiated into macrophages by treating with phorbol myristate acetate (PMA), which induces significant changes in cellular morphology and expression of cell surface markers characteristic of macrophages [46–48]. THP-1 cells, which typically grow in suspension, become adherent within 72 h post PMA treatment, providing a straightforward method for isolating relatively pure populations of non-differentiated monocytes and THP-1-derived macrophages . Isolation of homogeneous cell populations is particularly appealing for the study of tRNA gene dynamics during cellular differentiation, as tDNAs, which are essential for biosynthesis, are likely to exhibit comparatively subtle changes in transcription.
Here we present integrated tDNA expression profiling, a method that combines RNAPIII occupancy mapping with biotin-capture of nascent, demethylated tRNAs. We apply this method to the study of dynamic tRNA gene regulation during macrophage development and further integrate these data with our recently described maps of 3D chromatin structures in the same cell types. Integrated tDNA expression profiling reveals domain-level and loop-based organization of transcription during cellular differentiation, as well as dynamic transcription factor (TF) binding coincident with changes in tDNA transcription, altogether revealing novel features of tRNA gene regulation.
Integrated tDNA expression profiling in THP-1 monocytes
In total, we estimate the expression for 610 tRNA genes currently present in the human genomic tRNA database (gtRNAdb, hg19) [52, 53]. Integrated tDNA expression levels show a bimodal distribution consistent with previous reports suggesting that nearly half of all tRNA genes are not occupied by RNA polymerase III, resulting in little or no transcription (Additional file 1: Figure S1b) [54–56]. To better characterize the environmental context of tRNA gene transcription, we further profiled chromatin accessibility at tRNA genes using an assay for transposase accessible chromatin (ATAC-seq), as well as the level of histone H3K27 acetylation (H3K27ac), a histone modification positively associated with transcription levels at both RNA polymerase II and RNA polymerase III genes (Fig. 1b) [3, 57–60]. Correlation analysis reveals statistically significant relationships for both H3K27ac and chromatin accessibility with integrated tDNA expression levels at individual tRNA genes (Fig. 1c). The strong relationship observed between tDNA transcription and ATAC-seq (Spearman’s rank correlation coefficient = 0.79, p < 10^-16) suggests that DNA accessibility may uniquely capture the activity of short, non-coding tRNA genes, which are depleted of nucleosomes within the gene body and promoter regions . Together, measures of tDNA accessibility and H3K27ac appropriately reflect the transcriptional activity of human tRNA genes and indicate that integrated tDNA expression profiling accurately captures nascent tRNA gene transcription levels.
Domain-level organization of human tRNA genes
We next considered whether the linear arrangement of tRNA genes into clusters and physical contact domains plays a role in the organization of tRNA gene transcription. Contact domains were annotated in THP-1 cells using the arrowhead algorithm as previously reported , which in total identifies more than 12,000 domains with enhanced contact frequency . Altogether, we identify 256 physical contact domains containing one or more tRNA genes, with an average size of 2.3 resident tRNA genes per domain and a maximum size of 33 tRNA genes per domain (Fig. 1d). However, domain mapping alone does not encompass the entire human genome and thus we performed parallel analysis of tDNA clustering to comprehensively compare the expression of all human tRNA genes (Additional file 1: Figure S1c). Clusters were analogously defined as regions of DNA with one or more tDNAs, using a maximum tDNA-tDNA distance threshold of 20 Kb. In total, we identify 277 individual tDNA clusters with an average size of 2.2 tRNA genes per cluster and a maximum size of 29 tRNA genes per cluster (Fig. 1d).
We find that the median expression level for tRNA genes increases with the number of neighboring tDNAs, leveling off at a cluster size of approximately four tRNA genes (Additional file 1: Figure S1d). Inspection of individual multi-tDNA clusters and physical contact domains further suggests that proximal tRNA genes may be expressed at similar levels (Additional file 1: Figure S1e, f). To systematically determine whether tRNA genes present in the same cluster or domain do share similar gene activity, we compared the range and interquartile range (IQR) of tDNA transcription with a model in which tRNA genes are randomly assigned with respect to cluster and domain occupancy. Indeed, we find significantly lower spread in tRNA gene expression values across all cluster and domain sizes (Fig. 1e, f, Additional file 1: Figure S1g), suggesting cluster- and domain-level organization of human tDNAs group genes with similar transcriptional activity. We further find that tRNA gene expression positively associates with the proximity of tDNAs to genes transcribed by RNA polymerase II (Fig. 1g). These results agree with previous observations indicating correlative proximity and activity between neighboring RNA polymerase II and RNA polymerase III genes [60, 62, 63], and altogether argue that the surrounding context is an important aspect of tRNA gene transcription.
tDNA organization, transcription, and codon usage in THP-1 cells
We observe a similar correlation between aggregate tRNA levels and the codon usage of the THP-1 transcriptome (Fig. 2d), further serving to validate the accuracy and quality of our integrated tDNA expression profiles. Transcription of tRNA genes is particularly better adjusted towards codon usage than gene count with respect to high copy number tDNAs (Additional file 1: Figure S2e). For example, tRNAAla-AGC and tRNAAsn-GTT represent two of the highest anticodon families in terms of estimated tRNA gene count, yet they decode moderately employed codons in THP-1 cells (Fig. 2c). The aggregate levels of nascent tRNAAla-AGC and tRNAAsn-GTT are instead better adjusted to codon usage (Fig. 2d), suggesting transcription of human tRNA genes is regulated beyond gene count.
Visualization of tRNA gene coordinates with respect to tRNA-type illustrates the level of overlap between distinct anticodon families across all human chromosomes (Fig. 2a). Hierarchical clustering of tRNA families by overlap frequencies, that is the number of times genes encoding two distinct tRNA-types are located within the same tDNA cluster, reveals preferential proximity between specific pairs of anticodon tRNA families (Additional file 1: Figure S2f). The tDNAs located within the VNTR on chromosome 1, for example, exhibit strong overlap frequencies and together represent several of the highest expressed tRNA species in THP-1 monocytes (Fig. 2b–d). Genes encoding tRNA-types with strong overlap frequencies tend to segregate by tDNA expression levels, reaffirming the important relationship between tRNA gene organization and transcription (Additional file 1: Figure S2f).
Dynamic transcription of tRNA genes during macrophage differentiation
The collective decrease in non-mitochondrial tDNA expression is consistent with previous comparisons of RNA polymerase III occupancy in human embryonic stem cells and induced pluripotent stem cells, which suggest that differentiation leads to a constricted RNAPIII repertoire . We find that downregulation of nascent tRNA levels is most pronounced for the highest expressed tRNA-types, suggesting macrophage differentiation decreases the dynamic range of tRNA availability in THP-1 cells (Fig. 3c, d). This decrease in the most abundant tRNA anticodon families correlates with a decline in codon usage for several of the most frequently employed codons (Fig. 3d, Additional file 1: Figure S3h), suggesting a potentially coordinated decrease in the dynamic range for both tRNA supply and mRNA demand. Thus, we speculate that cell-type-specific tRNA levels may be adjusted in a manner that complements the dynamic range of codon usage rather than specific codon frequency optimization.
tDNA dynamics mirror the surrounding transcriptional environment of tRNA genes
Given the relationship between tDNA activity and proximity to RNA polymerase II genes, we next asked whether changes in tRNA gene expression coincide with the transcriptional environment surrounding differential tRNA genes. Indeed, tDNAs that decrease or increase significantly in THP-1-derived macrophages show enrichment for similar changes in nearby protein-coding genes (Fig. 3e, Additional file 1: Figure S3e). Beyond RNAPII transcribed genes, tDNAs also share similar transcriptional dynamics with tRNA genes that reside within the same cluster or topological domain. When tDNA transcription is compared with the median fold change across tDNA clusters and domains, increasing and decreasing tRNA genes again are biased towards genes the behave similarly, both for tDNA clusters (Fig. 3f) and for physical contact domains containing tRNA genes (Fig. 3g, Additional file 1: Figure S3f, g). Visual inspection of transcriptional and chromatin dynamics within specific tRNA domains and clusters illustrates that dynamic tDNA expression corresponds to changes in the surrounding environment. tRNA genes co-residing in clusters and contact domains within 200 Kb on chromosome 5, for example, exhibit similar transcriptional dynamics, both with nearby tRNA genes encoding distinct tRNA-types and with RNAPII-transcribed genes (Fig. 3a). A general decline in chromatin accessibility, H3K27 acetylation, and occupancy by RNA polymerase III is also observed across this locus, together suggesting that the topological organization of tDNAs within these physical contact domains may contribute to their expression and dynamic regulation during cellular differentiation.
Coordinated long-range regulation of tRNA genes during cellular differentiation
We next asked what features are connected to tRNA genes by DNA loops and whether dynamic tDNA expression levels are coordinated by long-range interactions. Loop anchors identified by Hi-C often interact with more than one distant locus, forming multi-interaction networks or “hubs” that, in certain cases, connect multiple enhancers to target genes . Analysis of all loop-associated downregulated tDNAs reveals a multi-interaction network that connects tRNA genes to other tDNAs as well as RNAPII-transcribed genes and intergenic enhancers marked by H3K27 acetylation (Fig. 4b). Features connected to tRNA genes by DNA loops show strikingly similar changes in transcription, suggesting tDNA dynamics are coordinated with other genes through long-range interactions during macrophage development (Fig. 4b). Visualization of individual tDNA interaction communities illustrates this phenomenon more clearly. For example, tRNA genes located at the boundary of adjacent tDNA contact domains on chromosome 5 (Fig. 3a) are directly located at a loop anchor that connects these tRNA genes to ZFP62, an RNA polymerase II-transcribed gene approximately 300 Kb upstream that concomitantly decreases in transcription in differentiating THP-1 cells (Fig. 4c, left and right signal track). DNA loops also bring ZFP62 in close spatial proximity to additional tDNAs that decrease in transcription and RNA polymerase III occupancy (Fig. 4c, middle signal track), altogether illustrating coordinated long-range transcriptional downregulation after treatment with PMA.
In contrast to downregulated tRNA genes, we find that tDNAs that increase in transcription during cellular differentiation form interaction networks with comparatively few genes and with genes and regulatory elements that do not show significant changes after treatment with PMA. However, we find that nuclear-encoded mitochondrial tRNA genes, which generally increase in expression in developing macrophages, are proximal to DNA loops that connect genes and enhancers that behave similarly after differentiation, further reaffirming an important relationship between long-range interactions and coordinated dynamic transcription of human tRNA genes (Additional file 1: Figure S4f, g).
Loop-based tRNA genes are connected by CTCF binding sites
Previous reports have identified enrichment for tRNA genes at the boundaries of topological domains, as well as a proximity relationship between specific tRNA genes and CTCF binding sites in eukaryotes [28, 62, 68–70]. Analysis of CTCF proximity with respect to tDNAs demonstrates that loop-associated tRNA genes are located near CTCF binding sites in THP-1 cells, consistent with recent models suggesting most long-range interactions are established by a loop extrusion complex that requires convergent CTCF binding sites (Fig. 4d) [36, 37, 40]. Inspection of DNA loops that bridge together tRNA genes clearly identifies CTCF binding at individual loop anchors, further suggesting that distal tRNA genes are brought together by CTCF (Fig. 4c). Supporting evidence for a functional role of CTCF in connecting tRNA genes is illustrated by an example in which two distinct clusters of tRNA genes, separated by more than 1.2 Mb on chromosome 6, interact via a DNA loop that is lost during macrophage differentiation, coincident with a loss of CTCF binding (Additional file 1: Figure S5). The tDNA clusters that are released by this long-range interaction are marked by decreasing tRNA gene transcription in THP-1 macrophages (Fig. 4b, asterisk), suggesting loss of DNA looping may be related to dynamic transcription regulation of tRNA genes.
Despite the observed relationship between tDNA transcription and CTCF-mediated long-range interactions, we do not identify any enrichment for differential tRNA genes at loop ends and, overall, changes in tDNA-associated long-range interactions are no more dynamic than non-tDNA loops (Additional file 1: Figure S4b, e). This suggests that while looping may be an important feature underlying tDNA expression levels, differentiation of THP-1 monocytes does not induce any widespread perturbation of the tDNA interactome in these cells. Indeed, regulation of tRNA genes is likely predominantly controlled by dynamic binding of TFs proximal to tDNAs within the framework of chromatin architecture. We therefore sought to further identify factors that might directly regulate tRNA gene expression during macrophage development.
Downregulation of tRNA genes coincides with enhanced MAF1 occupancy
Transcription factor footprinting uncovers candidate regulators of tDNA expression
The ends of read fragments generated by ATAC-seq can be used to identify regions of DNA that are directly bound by TFs and protected against fragmentation [73, 74]. To this end, we applied the Protein Interaction Quantification (PIQ) footprinting algorithm on ATAC-seq data generated in THP-1 cells, identifying in total more than 2 million footprints for 516 distinct TF motifs (Additional file 1: Figure S6b) [75, 76]. TF binding analysis identifies enrichment for specific regulatory elements within tDNA domains and tDNA clusters. As expected, both the A-BOX and B-BOX motifs, which represent internal tDNA regulatory elements bound by the TF complex TFIIIC , are substantially more enriched in tDNA clusters and tDNA contact domains than any other regulatory motif (Fig. 5e). Beyond TFIIIC binding sites, we also identify enrichment for several non-tDNA related regulatory elements. We therefore sought to further compare changes in TF binding during cellular differentiation with dynamic transcription of tRNA genes.
We observe a strong correlation between dynamic H3K27ac, a mark of active enhancers and promoters, with changes in chromatin accessibility over all TF footprints identified by PIQ (Fig. 5f) [78, 79]. ChIP-seq measurements of CTCF binding at intersecting motifs further validates the ability of this approach to capture dynamic binding of specific TFs (Additional file 1: Figure S6c). Analysis of dynamic chromatin accessibility for footprints enriched within tDNA domains and clusters uncovers strong correlations between TF occupancy and changes in nearby tRNA genes for specific motifs. For example, differential accessibility at ARNT::HIF1A footprints correlates with changes in tDNA transcription at proximal tRNA genes (Fig. 5g). Bound ARNT::HIF1A elements are strongly enriched in both tDNA clusters and contact domains, supporting a potential role for these factors in tDNA transcription regulation (Fig. 5e). We additionally observe correlations between tDNA transcription and footprint dynamics at HINFP and EGR1 regulatory elements (Fig. 5h, i), which are also enriched within tDNA clusters and domains. The expression of HIF1A and EGR1, both of which have been shown to significantly increase in PMA-treated THP-1 cells [80, 81], are also upregulated in our system after cellular differentiation (Fig. 5a), further validating the dynamic binding captured at these specific regulatory elements and supporting a possible role for these factors in dynamic tRNA gene regulation.
At the local level, downregulation of specific tRNA genes coincides with enhanced binding by MAF1, a transcriptional repressor that actively targets the promoter of RNAPIII genes [71, 82–88]. Though ectopic knock-down of MAF1 in human IMR90 fibroblasts leads to enhanced RNAPIII occupancy at all expressed tRNA genes, we show that MAF1 shows preferential enrichment after THP-1 differentiation at specific, downregulated tDNAs, consistent with differential sensitivity of tRNA genes to Maf1 in yeast [71, 72]. TF footprinting analyses in THP-1 monocytes and macrophages also reveal enrichment for specific DNA motifs located near tRNA genes (Fig. 5e). Furthermore, dynamic binding at certain motifs correlates with differential tDNA expression patterns during differentiation, suggesting a potential role in tRNA gene activity. Regulatory elements targeted by ARNT::HIF1A, for example, are enriched within tDNA clusters and domains and binding dynamics at these elements correlate with tRNA gene dynamics in THP-1 cells. Hypoxic stress, which induces the accumulation and nuclear translocation of ARNT and HIF1A, has been shown to suppress RNAPIII recruitment and tDNA expression in cardiomyocytes and increase levels of tRNA-derived fragments in breast cancer and mammary epithelial cells [7, 89–91]. Thus, whether and to what degree these factors directly and actively influence the level of nascent cellular tRNAs remains an intriguing subject for future research .
Our study suggests that the 3D organization of the human genome is an important underlying feature of tRNA gene expression. A subset of tDNAs participates in long-range interactions and is expressed at significantly elevated levels compared to non-loop tDNAs. Importantly, tRNA genes that are proximal or intersect DNA loop anchors are strongly enriched for CTCF and inspection of several tDNA-associated interaction sites reveals that CTCF, rather than the tRNA genes themselves, are centrally enriched at DNA loop anchors. This result suggests that while tRNA genes and sites bound by the TFIIIC TF complex may contribute to long-range chromosome organization and subnuclear localization in eukaryotes, in humans, loop-based cis-interactions appear to be predominantly determined by CTCF and factors controlling cohesin association with DNA [35, 41, 42, 44, 92–97].
Overall, DNA loops bridging tRNA genes to distal tDNAs and RNAPII transcribed genes are relatively stable after PMA-induced differentiation. We find that downregulated tDNAs interact with genomic features that are similarly downregulated, suggesting tDNAs and non-tRNA genes are coordinately regulated within the framework of a stable chromatin architecture established by CTCF. This result is consistent with a minimal change in CTCF binding observed during THP-1 differentiation and the predominant role of static rather than dynamic loops in bridging dynamic enhancers to key regulatory genes during macrophage development . Nevertheless, we do observe specific examples of dynamic tDNA-associated loops, such as a tDNA–tDNA interaction on chromosome 6 that is concomitantly lost and marked by decreasing tRNA gene expression during differentiation (Addition file 5: Figure S5). Loss of this long-range interaction coincides with diminished CTCF binding, reaffirming the important role of CTCF in establishing DNA loops connecting tRNA genes. This result is consistent with a recent manuscript suggesting that knockdown of either CTCF or cohesin subunit Rad21 leads to dynamic expression of human tRNA genes in mouse tail fibroblasts .
The dynamic expression of individual tRNA genes during cellular differentiation supports a more complicated structure governing nascent tRNA levels than simply tDNA copy number. Instead, differential transcription of human tRNA genes may provide a system for refining cellular tRNA pools towards cell-type-specific demands, consistent with recent examples of tRNA dynamics in response to specific perturbations [4, 5, 99]. In THP-1 cells, the decrease in nascent tRNA levels is likely harmonized with a slow-down in biosynthesis, as differentiation is accompanied by a loss in cellular proliferation and decreasing levels of regulatory kinases that control entry and progression through the cell cycle [100, 101]. It is worth noting that we do not observe any significant correlation between changes in the tRNA-type abundance and codon usage, as might be expected if tDNA dynamics were specially tuned toward cell-type-specific codon usage (Additional file 1: Figure S7a). This result is perhaps consistent with a recent report suggesting translational efficiency is stable across diverse cell types, regardless of cellular tRNA pools . Instead, we find that decreasing tDNA expression most significantly diminishes the level of highly expressed tRNAs, a phenomenon that appears to be coordinated with a decrease in codon usage for the most frequently employed codons (Additional file 1: Figure S7b, c). We therefore speculate that the range of tDNA expression may be coupled to the dynamic range in mRNA levels and resulting codon usage frequencies in different cell types, rather than specific anticodon-codon optimization. While the present study addresses dynamics of tRNA synthesis at the transcriptional level, to what degree these changes impact the cellular levels of mature, aminoacyl-tRNAs available for translation remains an important question for future research.
The transcription of tRNA genes, which are linearly organized into clusters and domains that share similar gene activity, generally decreases in developing macrophages, coincident with a decline in the dynamic range of transcriptomic codon usage. We find that downregulation of tRNA genes often occurs across topological domains and coordinately with other tRNA genes and RNA polymerase II-transcribed genes connected by DNA loops. We also show that MAF1, a negative effector of RNA polymerase III activity, increases at significantly downregulated tRNA genes during cellular differentiation, altogether revealing multiple levels of tDNA regulation during macrophage development.
THP-1 cell culture and differentiation
THP-1 cells were obtained from ATCC (Lot # 62454382) and grown for multiple passages in T-75 flasks of 2–8 × 105 cells/mL in growth medium containing RPMI-1640 (Corning), 10% fetal bovine serum, and 1% penicillin streptomycin. For differentiation of THP-1 cells, non-adherent cells were diluted to 2 × 105 cells/mL and grown overnight; a final concentration of 100 nM PMA was added the following morning. THP-1-derived macrophages were collected after 72-h exposure to PMA by aspirating media and any non-adherent cells and incubating adherent cells with TrypLE (ThermoFisher) for 15 min followed by cell wash in phosphate buffer saline (PBS) buffer.
Chromatin immunoprecipitation (ChIP)
Equal numbers of THP-1 monocytes and THP-1-derived macrophages were collected (~10 million cells per ChIP experiment) and resuspended in growth media at 1 × 106 cells/mL and cross-linked with rotation at room temperature in 1% formaldehyde for 10 min. Cross-linking was quenched with the addition of 200 mM glycine and an additional 5 min of rotation at room temperature. Cross-linked cells were then spun down and resuspended in 1× RIPA lysis buffer, followed by chromatin shearing via sonication (three cycles using a Branson sonicator: 30 s on, 60 s off; 15 additional cycles on a Bioruptor sonicator: 30 s on, 30 s off). Individual ChIP experiments were performed on pre-cleared chromatin using antibody-coupled Dynabead protein G (ThermoFisher) magnetic beads. Anti-histone H3 (acetyl K27) antibody was obtained from Abcam (ab4729), POLR3D antibody was obtained from abcam (ab86786; Lot# GR267691-1), MAF1 antibody was obtained from Santa Cruz Biotechnology (sc-365312 lot # G1411), and CTCF antibody was obtained from Millipore (07-729). A total of 3–5 ug of antibody per ChIP was coupled to 18-uL beads and rotated overnight with sheared chromatin at 4 °C. Beads were then washed 5× in ChIP wash buffer (Santa Cruz), 1× in TE, and chromatin eluted in TE + 1% SDS. Cross-linking was then reversed by incubation at 65 °C overnight, followed by digestion of RNA (30 min RNase incubation at 37 °C) and digestion of protein (30 min proteinase K incubation at 45 °C). ChIP DNA was then purified on a minElute column (Qiagen), followed by DNA library preparation and size selection of 350–550 bp fragments via gel extraction (Qiagen).
Assay for transposase accessible chromatin (ATAC-seq)
Equal numbers of THP-1 monocytes and THP-1-derived macrophages were collected (50,000 cells per ATAC-seq experiment) and washed with 1× ice-cold PBS. Cells were pelleted via centrifugation (500 × g, 5 min, 4 °C) and resuspended in cell lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630), and immediately spun down (500 × g, 10 min, 4 °C). The supernatant was then discarded, and transposition reaction carried out for 30 min at 37 °C with Tn5 transposase in transposition buffer (Illumina, cat#FC-121-1030). DNA was immediately purified on a minElute column (Qiagen), followed by PCR amplification using the NEBNext high-fidelity master mix (NEB cat#M0541) with nextera PCR primers and barcodes. PCR amplification was monitored as described , and gel purified to remove contaminating primer-dimer species.
Biotin-capture of nascent RNA
Equal numbers of THP-1 monocytes and THP-1-derived macrophages were collected (~5 million cells per experiment) and washed 3× in ice-cold PBS, followed by resuspension and 5 min incubation in 10 mL ice-cold swelling buffer (10 mM Tris-Cl pH 7.5, 2 mM MgCl2, 3 mM CaCl2). Cells were pelleted at 4 °C and resuspended in 1 mL lysis buffer (swelling buffer + 0.5% Igepal, 10% glycerol, 2 u/mL SUPERase In (Ambion), and gently mixed 20× by pipetting with p1000 (pipette tip cutoff to reduce shearing). Nuclei were then pelleted (1000 × g) and washed once with lysis buffer, pelleted (1000 × g), and resuspended in 1 mL nuclear storage buffer (50 mM Tris-Cl pH 8.3, 40% glycerol, 5 mM MgCl2, 100 nM EDTA). Nuclei were again pelleted and resuspended in 100 uL nuclear storage buffer. Nuclei were mixed with an equal volume of run-on reaction buffer (10 mM Tris-Cl pH 8.0, 5 mM MgCl2, 1 mM DTT, 300 mM KCl, 20 u SUPERase In (Ambion), 1% Sarkosyl, + 0.375 mM biotin-11-C/UTP (Perkin-Elmer)) + 0.0375 mM biotin-11-A/GTP (Perkin-Elmer)), and incubated for 3 min at 30 °C. RNA was then extracted and isolated using the mirVana small RNA isolation kit (AM1560; Lot# 1412093). A total of 1 ug of purified small RNA was then incubated for 2 h at room temperature in 100 uL demethylation reaction buffer (600 mM KCl, 4 mM MgCl2, 100 uM NH4FeSO4, 600 uM α-ketoglutarate, 4 mM L-ascorbic acid, 100 ug/mL bovine serum albumin [BSA], and 100 mM MES buffer [pH 5]) with 80 pmol ALKB, 160 pmol ALKB D135S. Expression and purification of tag-free ALKB (Lot# 716634S01) and ALKB D135S (Lot# 711466S04) was carried out by GenScript (Piscataway, NJ, USA) and stored at ~ 0.2–0.5 mg/mL in 50 mM Tris-HCl, 150 mM NaCl, 10% Glycerol, pH 8.0. Demethylation experiments were quenched in 10 mM EDTA, followed by pull-down of nascent biotinylated RNA via streptavidin magnetic beads (NEB #S1420S). Beads were washed in high salt buffer (2 M NaCl, 50 mM Tris-Cl pH 7.4, 0.5% Triton X-100), medium salt buffer (300 mM NaCl, 10 mM Tris-Cl pH 7.4, 0.1% Triton X-100), and low salt buffer (5 mM Tris-Cl pH 7.4, 0.1% Triton X-100), and RNA library preparation was subsequently carried out on beads, using the NEBnext small RNA library preparation kit (NEB #E7330S/L) with the minor modifications that RNA was denatured at 80 °C prior to adaptor ligation, and reverse transcription was carried out at increasing temperatures (50 °C for 1 h, 60 °C for 30 min, 70 °C for 15 min). Following PCR amplification (12 cycles), DNA library was purified on a minElute column and subsequently size selected to remove primer dimer contamination.
DNA sequencing and pre-processing
Biological replicates and experimental conditions (– PMA; + PMA) were sequenced together on an Illumina HiSeq2500 (paired-end, 100) for each individual experiment type (RNA-seq, ChIP-seq, biotin-capture) and sequencing reads trimmed using trim galore v. 0.4.0 (https://www.bioinformatics.babraham.ac.uk/projects/trim_galore/), before downstream sequence alignment and analyses.
Integrated tDNA expression profiling
tRNA gene annotation and coordinates were collected from the genomic tRNA database (gtRNAdb) for Homo sapiens (hg19 – NCBI Build 37.1 Feb 2009) by downloading the tRNAscan-SE results bed file http://gtrnadb.ucsc.edu/genomes/eukaryota/Hsapi19/ [52, 53]. Mapping of tRNA fragments to tDNA coordinates nevertheless remains an imperfect process due to the multi-copy nature of many tRNA genes. Multiple alignment and analysis strategies have been proposed with distinct decision-making trees [50, 51]. For our specific interest in tDNA transcription profiling, we chose to map nascent RNA reads to the entire genome space to avoid false positives arising from sequence reads that are unrelated to tRNAs, which may occur when aligned to a limited reference set of tRNA genes . In addition, the biotin-capture protocol ensures that tRNA fragments represent nascently transcribed RNA and thus special consideration of pre-tRNA and processed tRNA fragments was unnecessary. The presence of non-templated “CCA” at the 3′ terminus of mature tRNAs was not considered within the context of tRNA gene transcription. We further chose to limit multi-mapping reads to a single “best” alignment location to avoid erroneously increasing tDNA transcription estimation (as would occur with multi-mapping) or decreasing tDNA transcription estimation (as would occur if all multi-mapping reads were discarded).
Specifically, sequencing reads were filtered to a minimum size of 18 bp and the first sequencing read aligned to the hg19 reference genome with bowtie version 1.1.1 using options –k 1 “best” . Aligned nascent RNA sequence reads were extracted over the coordinates of all tRNA genes, ± 100 bp, in addition to all annotated genes. Normalized read counts for each condition replicate were determined with the DESeq package for differential expression, using the estimateSizeFactors function on count datasets and counts function with option normalized = TRUE . For integrated tDNA expression measurement, the mean normalized counts over tRNA genes, determined independently by biotin-capture RNA-seq and POLR3D ChIP-seq experiments, were taken for each condition and replicate. Integration of these two independent measures, which shows strong agreement (Fig. 1a), was chosen to benefit from the unique advantages of each assay. Importantly, inspection of integrated tDNA expression estimates demonstrates high correlation and reproducibility across biological replicate both before and after treatment with PMA (Additional file 1: Figure S1a). Nevertheless, in some cases, discrepancies in RNAPIII occupancy and nascent tRNA levels are observed for a subset of tDNAs (Fig. 1a). These differences potentially arise from differences in mappability, technical challenges specific to tRNA-seq, or to the indirect nature between POLR3D occupancy mapping, which alone may not equate to productive or efficient transcription of a given (tRNA) gene. We believe these differences give merit to the need for integrated tDNA expression profiling. Changes in tRNA gene expression levels before and after PMA treatment were determined as the mean fold change of integrated tDNA transcription across two biological replicates. tRNA genes that were considered downregulated and upregulated after 72 h PMA treatment show congruent changes in independent biotin-capture and RNAPIII mapping experiments with a false discovery rate (FDR) threshold of 0.15 (FDR determined using the Benjamini and Hochberg corrected exact test p value against integrated tDNA expression estimates).
Trimmed paired-end ChIP sequencing reads were mapped to the hg19 genome using bowtie version 1.1.1. with settings “bowtie –q –phred33-quals –X 2000 –fr –p 9 –S –chunkmbs 400” . Mitochondrial reads were filtered and duplicate reads removed using Picard tools v. 1.92 (http://broadinstitute.github.io/picard). ChIP-seq peaks were identified for each condition using MACS2 v. 2.1.0 with options “macs2 callpeak –bdg –t –g hs” . POLR3D ChIP-seq reads were extracted over tRNA genes, ± 100 bp, as well as all non-tRNA genes before normalization and integration with biotin-capture experiments for tDNA expression profiling. MAF1 binding dynamics were determined by differential count analysis over a merged MAF1 peak list using the exactTest function in the edgeR package for differential expression analysis [106, 107].
ATAC-seq and TF footprinting analysis
Trimmed ATAC sequencing reads were mapped to the hg19 genome using Bowtie v 2.2.4 with settings “bowtie2 –t –sensitive” . Mapped reads were merged across several sequencing replicates, before filtering mitochondrial reads and removing duplicate reads with Picard tools v. 1.92 (http://broadinstitute.github.io/picard). ATAC-seq peaks were identified for each condition using MACS2 v. 2.1.0 with options “macs2 callpeak –bdg –nomdel –t –g hs” . Changes in chromatin accessibility were determined by differential read count analysis using the glmTreat function in edgeR over a merged list of peaks identified in each condition and biological replicate. TF footprinting on ATAC-seq data was broken into two steps: identifying bound TF motifs and determining the differential binding score at motifs bound in either or both conditions. Bound TF motifs were identified using the PIQ pipeline against motifs annotated in the Jaspar Core Vertebrate Database (http://jaspar.genereg.net) [75, 76]. Motif matches against the hg19 reference genome were identified using the PIQ package pwmmatch.exact.r script. TF footprint scores were then determined for each motif using the PIQ package pertf.bg.r and common.r scripts with default settings. Motifs that were considered bound were filtered at a minimum positive prediction value (PPV) of 0.7, as previously described for bound motif calling . Differential TF binding scores for each motif were then determined using the Wellington-bootstrap algorithm for differential footprinting , using the bootstrap.py script for differential footprinting with command-line option “-A” for ATAC-seq input, followed by the pyDNase dnase_ddhs_scorer.py script for differential score calling over footprints identified by PIQ. Differential accessibility scores were median normalized and subsequently binned by standard deviation.
Signal track and data visualization
Signal track data were generated from post-filtering read alignment bam files using the deeptools bamCompare tool [109, 110]. For individual sample conditions, normalized read per genomic count (RPGC) signal tracks were created by taking the mean ratio between biological replicates with bamCompare options “--ratio mean --normalizeTo1x 2451960000 --binSize 20 --smoothLength 60”. For ± PMA treatment comparison tracks, signal files were generated with bamCompare options “--pseudocount 30 --normalizeTo1x 2451960000 --binSize 20 --smoothLength 60”. Read density plots were generated using the deeptools computeMatrix tool with specified distances from strand-oriented tRNA genes [109, 110]. Signal track visualization and in situ Hi-C integration plots were generated using the Sushi package for genomic visualization . Genome-wide tRNA gene circle visualization plots were generated using the RCircos package for Circos 2D track plots . tDNA interactome network maps were generated using the R package iGraph for network analysis and visualization .
tDNA cluster, domain, and loop calling
Clusters of tRNA genes were defined as a stretch of tRNA genes separated by a maximum tDNA-tDNA distance of 20 Kb. In other words, tDNAs within 20 Kb of another tRNA gene were grouped, with cluster size increasing until no remaining tRNA genes were within the specified distance. In situ Hi-C contact domains were defined in THP-1 monocytes using the previously described arrowhead algorithm at 5-Kb resolution with default Juicer parameters . In total, 12,272 contact domains were identified . tDNA domains were defined as any Hi-C contact domain, profiled in THP-1 cells by high-throughput chromosome conformation capture , that contains any tRNA gene(s). tRNA clusters and domains were scored by the number of resident tRNA genes. In some cases, contact domains are located within a larger overlapping contact domain. Thus, certain analyses avoid redundant tDNA-domain calling by assigning individual tRNA genes to the smallest resident tDNA contact domain. On the other hand, several tRNA genes are not within an identifiable contact domain and thus parallel analysis of tDNA clustering presents an analogous means of determining the role of proximal tDNA gene regulation. Loop-associated tRNA genes were defined as tRNA genes that intersect either end of a loop (10-Kb resolution), and comparisons of distance between tRNA genes and long-range interactions calculated as the shortest distance between individual tDNAs and loop ends (bedtools).
Intra-cluster, intra-domain, and tDNA-interactome expression analyses
Intra-cluster range and IQR of integrated tDNA expression levels were determined for each unique tDNA cluster. tRNA genes were then randomly shuffled with respect to tDNA cluster assignment and the range and IQR permuted 100,000 times. Observed and expected ranges were compared across all clusters and domains (Additional file 1: Figure S1g) and with respect to cluster and domain size (Fig. 1e, f). In situ Hi-C contact domains containing tRNA genes were analyzed for intra-domain tDNA expression range and IQR in the same manner, with the exception that in cases where tRNA genes are present in multiple overlapping contact domains, these tDNAs were assigned to the single, smallest intersecting domain to avoid redundancy. tDNA interactome network analysis was generated using the iGraph R package for network analysis and visualization . A graph object was created for all DNA loops mapped in THP-1 cells  with vertices representing loop anchors connected by edges (loops). All sub-network looping communities were detected using the fast greedy algorithm; communities that contain tRNA genes of interest (i.e. downregulated, upregulated, nmt-tDNA, etc.) were extracted for further analysis. All extracted community vertices were then characterized by intersecting or proximal features assigned by a ranking system: (1) intersecting tRNA genes; (2) proximal tRNA genes within four 10-Kb bins of a vertex; (3) non-tRNA genes that intersect a vertex; (4) non-tRNA genes that are proximal within two 10-Kb bins of a vertex; and (5) intergenic H3K27 acetylation peaks (putative enhancers) that directly intersect a vertex. Vertex shapes were determined by the highest ranked feature (tRNA genes = squares; non-tRNA genes = circles; putative enhancers = triangles), and the vertex shape and color scaled by the median log2(fold change) of the highest ranked feature(s).
Calculation of median haploid tRNA gene copy number
We utilized a read depth approach to estimate the median tRNA gene copy number in humans. Specifically, whole-genome sequencing data for 15 individuals in the 1000 Genomes Project (six high-coverage genomes: NA12878, NA12891, NA12892, NA19240, NA19239, NA19238; nine moderate-coverage genomes: NA10847, NA12890, NA18489, NA18499, NA18504, NA18505, NA18519, NA19098, and NA19099) were mapped to the hg19 reference genome. Read coverage over individual tRNA genes were compared to 1000 randomly shuffled blocks of the same length to determine background coverage. Read coverage was then collapsed by anticodon tRNA families and tRNA gene count estimated by comparison to collapsed randomized background coverage (Additional file 1: Figure S2a).
Illumina sequencing services were performed by the Stanford Center for Genomics and Personalized Medicine. We thank members of the Snyder lab for insightful discussion and feedback.
KVB is supported by National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) grant F32DK107112. DHP is supported by National Institutes of Health, National Human Genome Research Institute (NHGRI) grant R00HG008662. This research was supported by NIH Centers of Excellence in Genomic Science (CEGS) grant 5P50HG00773502, NIH NHGRI grant 5U01HG007919-03, and NIH grant 3U54HG00699604S1.
Availability of data and materials
The sequencing data from this study are publicly available under GEO series number GSE96800 through the NCBI Gene Expression Omnibus (GEO; http://www.ncbi.nlm.nih.gov/geo/). Previously reported in situ Hi-C data  is publicly available through Sequencing Read Archive (SRA) bioproject number PRJNA385337. Processed Hi-C data are available through the visualization software tool Juicebox (http://www.aidenlab.org/juicebox/). TF footprinting scripts were obtained from https://bitbucket.org/thashim/piq-single (PIQ R scripts pwmmatch.exact.r, pairedbam2rdata.r, pertf.bg.r and common.r) and http://pythonhosted.org/pyDNase/ (Wellington-bootstrap python scripts wellington-bootstrap.py and dnase_ddhs_scorer.py).
KVB, DHP, and MPS conceived the research project. KVB and DHP carried out molecular experiments and bioinformatic analyses. KVB and MPS wrote the manuscript. All authors read and approved the final manuscript.
Ethics approval and consent to participate
MPS is a founder and member of the science advisory board of Personalis and Qbio and a science advisory board member of Genapsys and Epinomics.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Qian W, Yang JR, Pearson NM, Maclean C, Zhang J. Balanced codon usage optimizes eukaryotic translational efficiency. PLoS Genet. 2012;8:e1002603.PubMedPubMed CentralView ArticleGoogle Scholar
- Novoa EM, Ribas de Pouplana L. Speeding with control: codon usage, tRNAs, and ribosomes. Trends Genet. 2012;28:574–81.PubMedView ArticleGoogle Scholar
- Gingold H, Tehler D, Christoffersen NR, Nielsen MM, Asmar F, Kooistra SM, et al. A dual program for translation regulation in cellular proliferation and differentiation. Cell. 2014;158:1281–92.PubMedView ArticleGoogle Scholar
- Pelechano V, Wei W, Steinmetz LM. Widespread co-translational RNA decay reveals ribosome dynamics. Cell. 2015;161:1400–12.PubMedPubMed CentralView ArticleGoogle Scholar
- Pang YL, Abo R, Levine SS, Dedon PC. Diverse cell stresses induce unique patterns of tRNA up- and down-regulation: tRNA-seq for quantifying changes in tRNA copy number. Nucleic Acids Res. 2014;42:e170.PubMedPubMed CentralView ArticleGoogle Scholar
- Schorn AJ, Gutbrod MJ, LeBlanc C, Martienssen R. LTR-Retrotransposon control by tRNA-derived small RNAs. Cell. 2017;170:61–71. e11.PubMedView ArticleGoogle Scholar
- Goodarzi H, Liu X, Nguyen HC, Zhang S, Fish L, Tavazoie SF. Endogenous tRNA-derived fragments suppress breast cancer progression via YBX1 displacement. Cell. 2015;161:790–802.PubMedPubMed CentralView ArticleGoogle Scholar
- Goodarzi H, Nguyen HCB, Zhang S, Dill BD, Molina H, Tavazoie SF. Modulated expression of specific tRNAs drives gene expression and cancer progression. Cell. 2016;165:1416–27.PubMedPubMed CentralView ArticleGoogle Scholar
- Phizicky EM, Hopper AK. tRNA biology charges to the front. Genes Dev. 2010;24:1832–60.PubMedPubMed CentralView ArticleGoogle Scholar
- Kutter C, Brown GD, Goncalves A, Wilson MD, Watt S, Brazma A, et al. Pol III binding in six mammals shows conservation among amino acid isotypes despite divergence among tRNA genes. Nat Genet. 2011;43:948–55.PubMedPubMed CentralView ArticleGoogle Scholar
- Schmitt BM, Rudolph KL, Karagianni P, Fonseca NA, White RJ, Talianidis I, et al. High-resolution mapping of transcriptional dynamics across tissue development reveals a stable mRNA-tRNA interface. Genome Res. 2014;24:1797–807.PubMedPubMed CentralView ArticleGoogle Scholar
- Roberts DN, Stewart AJ, Huff JT, Cairns BR. The RNA polymerase III transcriptome revealed by genome-wide localization and activity-occupancy relationships. Proc Natl Acad Sci U S A. 2003;100:14695–700.PubMedPubMed CentralView ArticleGoogle Scholar
- Cozen AE, Quartley E, Holmes AD, Hrabeta-Robinson E, Phizicky EM, Lowe TM. ARM-seq: AlkB-facilitated RNA methylation sequencing reveals a complex landscape of modified tRNA fragments. Nat Methods. 2015;12:879–84.PubMedPubMed CentralView ArticleGoogle Scholar
- Zheng G, Qin Y, Clark WC, Dai Q, Yi C, He C, et al. Efficient and quantitative high-throughput tRNA sequencing. Nat Methods. 2015;12:835–7.PubMedPubMed CentralView ArticleGoogle Scholar
- Liu F, Clark W, Luo G, Wang X, Fu Y, Wei J, et al. ALKBH1-mediated tRNA demethylation regulates translation. Cell. 2016;167:1897.PubMedView ArticleGoogle Scholar
- Hrabeta-Robinson E, Marcus E, Cozen AE, Phizicky EM, Lowe TM. High-throughput small RNA sequencing enhanced by AlkB-facilitated RNA de-methylation (ARM-Seq). Methods Mol Biol. 2017;1562:231–43.PubMedPubMed CentralView ArticleGoogle Scholar
- Phizicky EM, Hopper AK. tRNA processing, modification, and subcellular dynamics: past, present, and future. RNA. 2015;21:483–5.PubMedPubMed CentralView ArticleGoogle Scholar
- Mahat DB, Kwak H, Booth GT, Jonkers IH, Danko CG, Patel RK, et al. Base-pair-resolution genome-wide mapping of active RNA polymerases using precision nuclear run-on (PRO-seq). Nat Protoc. 2016;11:1455–76.PubMedPubMed CentralView ArticleGoogle Scholar
- Jordan-Pla A, Gupta I, de Miguel-Jimenez L, Steinmetz LM, Chavez S, Pelechano V, et al. Chromatin-dependent regulation of RNA polymerases II and III activity throughout the transcription cycle. Nucleic Acids Res. 2015;43:787–802.PubMedView ArticleGoogle Scholar
- Jordan-Pla A, Miguel A, Serna E, Pelechano V, Perez-Ortin JE. Biotin-Genomic Run-On (Bio-GRO): A high-resolution method for the analysis of nascent transcription in yeast. Methods Mol Biol. 2016;1361:125–39.PubMedView ArticleGoogle Scholar
- James Faresse N, Canella D, Praz V, Michaud J, Romascano D, Hernandez N. Genomic study of RNA polymerase II and III SNAPc-bound promoters reveals a gene transcribed by both enzymes and a broad use of common activators. PLoS Genet. 2012;8:e1003028.PubMedPubMed CentralView ArticleGoogle Scholar
- Symmons O, Uslu VV, Tsujimura T, Ruf S, Nassari S, Schwarzer W, et al. Functional and topological characteristics of mammalian regulatory domains. Genome Res. 2014;24:390–400.PubMedPubMed CentralView ArticleGoogle Scholar
- Lupianez DG, Kraft K, Heinrich V, Krawitz P, Brancati F, Klopocki E, et al. Disruptions of topological chromatin domains cause pathogenic rewiring of gene-enhancer interactions. Cell. 2015;161:1012–25.PubMedPubMed CentralView ArticleGoogle Scholar
- Hnisz D, Day DS, Young RA. Insulated neighborhoods: structural and functional units of mammalian gene control. Cell. 2016;167:1188–200.PubMedView ArticleGoogle Scholar
- Dowen JM, Fan ZP, Hnisz D, Ren G, Abraham BJ, Zhang LN, et al. Control of cell identity genes occurs in insulated neighborhoods in mammalian chromosomes. Cell. 2014;159:374–87.PubMedPubMed CentralView ArticleGoogle Scholar
- Symmons O, Pan L, Remeseiro S, Aktas T, Klein F, Huber W, et al. The Shh topological domain facilitates the action of remote enhancers by reducing the effects of genomic distances. Dev Cell. 2016;39:529–43.PubMedPubMed CentralView ArticleGoogle Scholar
- Andrey G, Montavon T, Mascrez B, Gonzalez F, Noordermeer D, Leleu M, et al. A switch between topological domains underlies HoxD genes collinearity in mouse limbs. Science. 2013;340:1234167.PubMedView ArticleGoogle Scholar
- Dixon JR, Selvaraj S, Yue F, Kim A, Li Y, Shen Y, et al. Topological domains in mammalian genomes identified by analysis of chromatin interactions. Nature. 2012;485:376–80.PubMedPubMed CentralView ArticleGoogle Scholar
- Nora EP, Lajoie BR, Schulz EG, Giorgetti L, Okamoto I, Servant N, et al. Spatial partitioning of the regulatory landscape of the X-inactivation centre. Nature. 2012;485:381–5.PubMedPubMed CentralView ArticleGoogle Scholar
- Rao SS, Huntley MH, Durand NC, Stamenova EK, Bochkov ID, Robinson JT, et al. A 3D map of the human genome at kilobase resolution reveals principles of chromatin looping. Cell. 2014;159:1665–80.PubMedView ArticleGoogle Scholar
- Darrow EM, Huntley MH, Dudchenko O, Stamenova EK, Durand NC, Sun Z, et al. Deletion of DXZ4 on the human inactive X chromosome alters higher-order genome architecture. Proc Natl Acad Sci U S A. 2016;113:E4504–4512.PubMedPubMed CentralView ArticleGoogle Scholar
- Dixon JR, Jung I, Selvaraj S, Shen Y, Antosiewicz-Bourget JE, Lee AY, et al. Chromatin architecture reorganization during stem cell differentiation. Nature. 2015;518:331–6.PubMedPubMed CentralView ArticleGoogle Scholar
- Zuin J, Dixon JR, van der Reijden MI, Ye Z, Kolovos P, Brouwer RW, et al. Cohesin and CTCF differentially affect chromatin architecture and gene expression in human cells. Proc Natl Acad Sci U S A. 2014;111:996–1001.PubMedView ArticleGoogle Scholar
- Heidari N, Phanstiel DH, He C, Grubert F, Jahanbani F, Kasowski M, et al. Genome-wide map of regulatory interactions in the human genome. Genome Res. 2014;24:1905–17.PubMedPubMed CentralView ArticleGoogle Scholar
- Van Bortle K, Nichols MH, Li L, Ong CT, Takenaka N, Qin ZS, et al. Insulator function and topological domain border strength scale with architectural protein occupancy. Genome Biol. 2014;15:R82.PubMedPubMed CentralView ArticleGoogle Scholar
- Sanborn AL, Rao SS, Huang SC, Durand NC, Huntley MH, Jewett AI, et al. Chromatin extrusion explains key features of loop and domain formation in wild-type and engineered genomes. Proc Natl Acad Sci U S A. 2015;112:E6456–6465.PubMedPubMed CentralView ArticleGoogle Scholar
- Nichols MH, Corces VG. A CTCF code for 3D genome architecture. Cell. 2015;162:703–5.PubMedPubMed CentralView ArticleGoogle Scholar
- Nora EP, Goloborodko A, Valton AL, Gibcus JH, Uebersohn A, Abdennur N, et al. Targeted degradation of CTCF decouples local insulation of chromosome domains from genomic compartmentalization. Cell. 2017;169:930–44. e922.PubMedView ArticleGoogle Scholar
- Haarhuis JHI, van der Weide RH, Blomen VA, Yanez-Cuna JO, Amendola M, van Ruiten MS, et al. The cohesin release factor WAPL restricts chromatin loop extension. Cell. 2017;169:693–707.PubMedPubMed CentralView ArticleGoogle Scholar
- Fudenberg G, Imakaev M, Lu C, Goloborodko A, Abdennur N, Mirny LA. Formation of chromosomal domains by loop extrusion. Cell Rep. 2016;15:2038–49.PubMedPubMed CentralView ArticleGoogle Scholar
- Snider CE, Stephens AD, Kirkland JG, Hamdani O, Kamakaka RT, Bloom K. Dyskerin, tRNA genes, and condensin tether pericentric chromatin to the spindle axis in mitosis. J Cell Biol. 2014;207:189–99.PubMedPubMed CentralView ArticleGoogle Scholar
- Raab JR, Chiu J, Zhu J, Katzman S, Kurukuti S, Wade PA, et al. Human tRNA genes function as chromatin insulators. EMBO J. 2012;31:330–50.PubMedView ArticleGoogle Scholar
- Ebersole T, Kim JH, Samoshkin A, Kouprina N, Pavlicek A, White RJ, et al. tRNA genes protect a reporter gene from epigenetic silencing in mouse cells. Cell Cycle. 2011;10:2779–91.PubMedPubMed CentralView ArticleGoogle Scholar
- Noma K, Cam HP, Maraia RJ, Grewal SI. A role for TFIIIC transcription factor complex in genome organization. Cell. 2006;125:859–72.PubMedView ArticleGoogle Scholar
- Phanstiel DH, Van Bortle K, Spacek DV, Hess G, Shamim MS, Machol I, et al. Static and Dynamic DNA Loops form AP-1-Bound Activation Hubs during Macrophage Development. Mol Cell. 2017. http://dx.doi.org/10.1016/j.molcel.2017.08.006.
- Tsuchiya S, Kobayashi Y, Goto Y, Okumura H, Nakae S, Konno T, et al. Induction of maturation in cultured human monocytic leukemia cells by a phorbol diester. Cancer Res. 1982;42:1530–6.PubMedGoogle Scholar
- Auwerx J. The human leukemia cell line, THP-1: a multifacetted model for the study of monocyte-macrophage differentiation. Experientia. 1991;47:22–31.PubMedView ArticleGoogle Scholar
- Daigneault M, Preston JA, Marriott HM, Whyte MK, Dockrell DH. The identification of markers of macrophage differentiation in PMA-stimulated THP-1 cells and monocyte-derived macrophages. PLoS One. 2010;5:e8668.PubMedPubMed CentralView ArticleGoogle Scholar
- Kouno T, de Hoon M, Mar JC, Tomaru Y, Kawano M, Carninci P, et al. Temporal dynamics and transcriptional control using single-cell gene expression analysis. Genome Biol. 2013;14:R118.PubMedPubMed CentralView ArticleGoogle Scholar
- Telonis AG, Loher P, Kirino Y, Rigoutsos I. Consequential considerations when mapping tRNA fragments. BMC Bioinf. 2016;17:123.View ArticleGoogle Scholar
- Selitsky SR, Sethupathy P. tDRmapper: challenges and solutions to mapping, naming, and quantifying tRNA-derived RNAs from human small RNA-sequencing data. BMC Bioinf. 2015;16:354.View ArticleGoogle Scholar
- Chan PP, Lowe TM. GtRNAdb: a database of transfer RNA genes detected in genomic sequence. Nucleic Acids Res. 2009;37:D93–97.PubMedView ArticleGoogle Scholar
- Chan PP, Lowe TM. GtRNAdb 2.0: an expanded database of transfer RNA genes identified in complete and draft genomes. Nucleic Acids Res. 2016;44:D184–189.PubMedView ArticleGoogle Scholar
- Canella D, Bernasconi D, Gilardi F, LeMartelot G, Migliavacca E, Praz V, et al. A multiplicity of factors contributes to selective RNA polymerase III occupancy of a subset of RNA polymerase III genes in mouse liver. Genome Res. 2012;22:666–80.PubMedPubMed CentralView ArticleGoogle Scholar
- Canella D, Praz V, Reina JH, Cousin P, Hernandez N. Defining the RNA polymerase III transcriptome: Genome-wide localization of the RNA polymerase III transcription machinery in human cells. Genome Res. 2010;20:710–21.PubMedPubMed CentralView ArticleGoogle Scholar
- Alla RK, Cairns BR. RNA polymerase III transcriptomes in human embryonic stem cells and induced pluripotent stem cells, and relationships with pluripotency transcription factors. PLoS One. 2014;9:e85648.PubMedPubMed CentralView ArticleGoogle Scholar
- Buenrostro JD, Giresi PG, Zaba LC, Chang HY, Greenleaf WJ. Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position. Nat Methods. 2013;10:1213–8.PubMedPubMed CentralView ArticleGoogle Scholar
- Buenrostro JD, Wu B, Chang HY, Greenleaf WJ. ATAC-seq: a method for assaying chromatin accessibility genome-wide. Curr Protoc Mol Biol. 2015;109:21–9.PubMedPubMed CentralGoogle Scholar
- Sagi D, Rak R, Gingold H, Adir I, Maayan G, Dahan O, et al. Tissue- and time-specific expression of otherwise identical tRNA genes. PLoS Genet. 2016;12:e1006264.PubMedPubMed CentralView ArticleGoogle Scholar
- Raha D, Wang Z, Moqtaderi Z, Wu L, Zhong G, Gerstein M, et al. Close association of RNA polymerase II and many transcription factors with Pol III genes. Proc Natl Acad Sci U S A. 2010;107:3639–44.PubMedPubMed CentralView ArticleGoogle Scholar
- Helbo AS, Lay FD, Jones PA, Liang G, Gronbaek K. Nucleosome positioning and NDR structure at RNA polymerase III promoters. Sci Rep. 2017;7:41947.PubMedPubMed CentralView ArticleGoogle Scholar
- Moqtaderi Z, Wang J, Raha D, White RJ, Snyder M, Weng Z, et al. Genomic binding profiles of functionally distinct RNA polymerase III transcription complexes in human cells. Nat Struct Mol Biol. 2010;17:635–40.PubMedPubMed CentralView ArticleGoogle Scholar
- White RJ. Transcription by RNA polymerase III: more complex than we thought. Nat Rev Genet. 2011;12:459–63.PubMedView ArticleGoogle Scholar
- Iben JR, Maraia RJ. tRNA gene copy number variation in humans. Gene. 2014;536:376–84.PubMedView ArticleGoogle Scholar
- Genomes Project Consortium, Abecasis GR, Auton A, Brooks LD, DePristo MA, Durbin RM, et al. An integrated map of genetic variation from 1,092 human genomes. Nature. 2012;491:56–65.View ArticleGoogle Scholar
- Darrow EM, Chadwick BP. A novel tRNA variable number tandem repeat at human chromosome 1q23.3 is implicated as a boundary element based on conservation of a CTCF motif in mouse. Nucleic Acids Res. 2014;42:6421–35.PubMedPubMed CentralView ArticleGoogle Scholar
- Melnik S, Deng B, Papantonis A, Baboo S, Carr IM, Cook PR. The proteomes of transcription factories containing RNA polymerases I, II or III. Nat Methods. 2011;8:963–8.PubMedPubMed CentralView ArticleGoogle Scholar
- Oler AJ, Alla RK, Roberts DN, Wong A, Hollenhorst PC, Chandler KJ, et al. Human RNA polymerase III transcriptomes and relationships to Pol II promoter chromatin and enhancer-binding factors. Nat Struct Mol Biol. 2010;17:620–8.PubMedPubMed CentralView ArticleGoogle Scholar
- Smith ST, Wickramasinghe P, Olson A, Loukinov D, Lin L, Deng J, et al. Genome wide ChIP-chip analyses reveal important roles for CTCF in Drosophila genome organization. Dev Biol. 2009;328:518–28.PubMedView ArticleGoogle Scholar
- Yuen KC, Slaughter BD, Gerton JL. Condensin II is anchored by TFIIIC and H3K4me3 in the mammalian genome and supports the expression of active dense gene clusters. Sci Adv. 2017;3:e1700191.PubMedPubMed CentralView ArticleGoogle Scholar
- Orioli A, Praz V, Lhote P, Hernandez N. Human MAF1 targets and represses active RNA polymerase III genes by preventing recruitment rather than inducing long-term transcriptional arrest. Genome Res. 2016;26:624–35.PubMedPubMed CentralView ArticleGoogle Scholar
- Turowski TW, Lesniewska E, Delan-Forino C, Sayou C, Boguta M, Tollervey D. Global analysis of transcriptionally engaged yeast RNA polymerase III reveals extended tRNA transcripts. Genome Res. 2016;26:933–44.PubMedPubMed CentralView ArticleGoogle Scholar
- Song L, Zhang Z, Grasfeder LL, Boyle AP, Giresi PG, Lee BK, et al. Open chromatin defined by DNaseI and FAIRE identifies regulatory elements that shape cell-type identity. Genome Res. 2011;21:1757–67.PubMedPubMed CentralView ArticleGoogle Scholar
- Gusmao EG, Allhoff M, Zenke M, Costa IG. Analysis of computational footprinting methods for DNase sequencing experiments. Nat Methods. 2016;13:303–9.PubMedView ArticleGoogle Scholar
- Sherwood RI, Hashimoto T, O’Donnell CW, Lewis S, Barkal AA, van Hoff JP, et al. Discovery of directional and nondirectional pioneer transcription factors by modeling DNase profile magnitude and shape. Nat Biotechnol. 2014;32:171–8.PubMedPubMed CentralView ArticleGoogle Scholar
- Mathelier A, Fornes O, Arenillas DJ, Chen CY, Denay G, Lee J, et al. JASPAR 2016: a major expansion and update of the open-access database of transcription factor binding profiles. Nucleic Acids Res. 2016;44:D110–115.PubMedView ArticleGoogle Scholar
- Male G, von Appen A, Glatt S, Taylor NM, Cristovao M, Groetsch H, et al. Architecture of TFIIIC and its role in RNA polymerase III pre-initiation complex assembly. Nat Commun. 2015;6:7387.PubMedPubMed CentralView ArticleGoogle Scholar
- Piper J, Assi SA, Cauchy P, Ladroue C, Cockerill PN, Bonifer C, et al. Wellington-bootstrap: differential DNase-seq footprinting identifies cell-type determining transcription factors. BMC Genomics. 2015;16:1000.PubMedPubMed CentralView ArticleGoogle Scholar
- He HH, Meyer CA, Chen MW, Jordan VC, Brown M, Liu XS. Differential DNase I hypersensitivity reveals factor-dependent chromatin dynamics. Genome Res. 2012;22:1015–25.PubMedPubMed CentralView ArticleGoogle Scholar
- Oda T, Hirota K, Nishi K, Takabuchi S, Oda S, Yamada H, et al. Activation of hypoxia-inducible factor 1 during macrophage differentiation. Am J Physiol Cell Physiol. 2006;291:C104–113.PubMedView ArticleGoogle Scholar
- Akuzawa N, Kurabayashi M, Ohyama Y, Arai M, Nagai R. Zinc finger transcription factor Egr-1 activates Flt-1 gene expression in THP-1 cells on induction for macrophage differentiation. Arterioscler Thromb Vasc Biol. 2000;20:377–84.PubMedView ArticleGoogle Scholar
- Boguta M. Maf1, a general negative regulator of RNA polymerase III in yeast. Biochim Biophys Acta. 2013;1829:376–84.PubMedView ArticleGoogle Scholar
- Rideout EJ, Marshall L, Grewal SS. Drosophila RNA polymerase III repressor Maf1 controls body size and developmental timing by modulating tRNAiMet synthesis and systemic insulin signaling. Proc Natl Acad Sci U S A. 2012;109:1139–44.PubMedPubMed CentralView ArticleGoogle Scholar
- Vannini A, Ringel R, Kusser AG, Berninghausen O, Kassavetis GA, Cramer P. Molecular basis of RNA polymerase III transcription repression by Maf1. Cell. 2010;143:59–70.PubMedView ArticleGoogle Scholar
- Gajda A, Towpik J, Steuerwald U, Muller CW, Lefebvre O, Boguta M. Full repression of RNA polymerase III transcription requires interaction between two domains of its negative regulator Maf1. J Biol Chem. 2010;285:35719–27.PubMedPubMed CentralView ArticleGoogle Scholar
- Ciesla M, Boguta M. Regulation of RNA polymerase III transcription by Maf1 protein. Acta Biochim Pol. 2008;55:215–25.PubMedGoogle Scholar
- Goodfellow SJ, Graham EL, Kantidakis T, Marshall L, Coppins BA, Oficjalska-Pham D, et al. Regulation of RNA polymerase III transcription by Maf1 in mammalian cells. J Mol Biol. 2008;378:481–91.PubMedView ArticleGoogle Scholar
- Rollins J, Veras I, Cabarcas S, Willis I, Schramm L. Human Maf1 negatively regulates RNA polymerase III transcription via the TFIIB family members Brf1 and Brf2. Int J Biol Sci. 2007;3:292–302.PubMedPubMed CentralView ArticleGoogle Scholar
- Dengler VL, Galbraith MD, Espinosa JM. Transcriptional regulation by hypoxia inducible factors. Crit Rev Biochem Mol Biol. 2014;49:1–15.PubMedView ArticleGoogle Scholar
- Ernens I, Goodfellow SJ, Innes F, Kenneth NS, Derblay LE, White RJ, et al. Hypoxic stress suppresses RNA polymerase III recruitment and tRNA gene transcription in cardiomyocytes. Nucleic Acids Res. 2006;34:286–94.PubMedPubMed CentralView ArticleGoogle Scholar
- Guimaraes-Camboa N, Stowe J, Aneas I, Sakabe N, Cattaneo P, Henderson L, et al. HIF1alpha represses cell stress pathways to allow proliferation of hypoxic fetal cardiomyocytes. Dev Cell. 2015;33:507–21.PubMedPubMed CentralView ArticleGoogle Scholar
- Mourad R, Li L, Cuvier O. Uncovering direct and indirect molecular determinants of chromatin loops using a computational integrative approach. PLoS Comput Biol. 2017;13:e1005538.PubMedPubMed CentralView ArticleGoogle Scholar
- Van Bortle K, Corces VG. tDNA insulators and the emerging role of TFIIIC in genome organization. Transcription. 2012;3:277–84.PubMedPubMed CentralView ArticleGoogle Scholar
- Kirkland JG, Raab JR, Kamakaka RT. TFIIIC bound DNA elements in nuclear organization and insulation. Biochim Biophys Acta. 2013;1829:418–24.PubMedView ArticleGoogle Scholar
- Haeusler RA, Pratt-Hyatt M, Good PD, Gipson TA, Engelke DR. Clustering of yeast tRNA genes is mediated by specific association of condensin with tRNA gene transcription complexes. Genes Dev. 2008;22:2204–14.PubMedPubMed CentralView ArticleGoogle Scholar
- Thompson M, Haeusler RA, Good PD, Engelke DR. Nucleolar clustering of dispersed tRNA genes. Science. 2003;302:1399–401.PubMedPubMed CentralView ArticleGoogle Scholar
- Duan Z, Andronescu M, Schutz K, McIlwain S, Kim YJ, Lee C, et al. A three-dimensional model of the yeast genome. Nature. 2010;465:363–7.PubMedPubMed CentralView ArticleGoogle Scholar
- Woolnough JL, Schneider DA, Giles KE. The regulation of tDNA transcription during the directed differentiation of stem cells. bioRxiv. 2017. https://doi.org/10.1101/108472.
- Van Bortle K, Nichols MH, Ramos E, Corces VG. Integrated tRNA, transcript, and protein profiles in response to steroid hormone signaling. RNA. 2015;21:1807–17.PubMedPubMed CentralView ArticleGoogle Scholar
- Schwende H, Fitzke E, Ambs P, Dieter P. Differences in the state of differentiation of THP-1 cells induced by phorbol ester and 1,25-dihydroxyvitamin D3. J Leukoc Biol. 1996;59:555–61.PubMedGoogle Scholar
- Richter E, Ventz K, Harms M, Mostertz J, Hochgrafe F. Induction of macrophage function in human THP-1 cells is associated with rewiring of MAPK signaling and activation of MAP3K7 (TAK1) protein kinase. Front Cell Dev Biol. 2016;4:21.PubMedPubMed CentralView ArticleGoogle Scholar
- Rudolph KL, Schmitt BM, Villar D, White RJ, Marioni JC, Kutter C, et al. Codon-driven translational efficiency is stable across diverse mammalian cell states. PLoS Genet. 2016;12:e1006024.PubMedPubMed CentralView ArticleGoogle Scholar
- Langmead B, Trapnell C, Pop M, Salzberg SL. Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol. 2009;10:R25.PubMedPubMed CentralView ArticleGoogle Scholar
- Anders S, Huber W. Differential expression analysis for sequence count data. Genome Biol. 2010;11:R106.PubMedPubMed CentralView ArticleGoogle Scholar
- Zhang Y, Liu T, Meyer CA, Eeckhoute J, Johnson DS, Bernstein BE, et al. Model-based analysis of ChIP-Seq (MACS). Genome Biol. 2008;9:R137.PubMedPubMed CentralView ArticleGoogle Scholar
- Nikolayeva O, Robinson MD. edgeR for differential RNA-seq and ChIP-seq analysis: an application to stem cell biology. Methods Mol Biol. 2014;1150:45–79.PubMedView ArticleGoogle Scholar
- Robinson MD, McCarthy DJ, Smyth GK. edgeR: a Bioconductor package for differential expression analysis of digital gene expression data. Bioinformatics. 2010;26:139–40.PubMedView ArticleGoogle Scholar
- Langmead B, Salzberg SL. Fast gapped-read alignment with Bowtie 2. Nat Methods. 2012;9:357–9.PubMedPubMed CentralView ArticleGoogle Scholar
- Ramirez F, Ryan DP, Gruning B, Bhardwaj V, Kilpert F, Richter AS, et al. deepTools2: a next generation web server for deep-sequencing data analysis. Nucleic Acids Res. 2016;44:W160–165.PubMedPubMed CentralView ArticleGoogle Scholar
- Ramirez F, Dundar F, Diehl S, Gruning BA, Manke T. deepTools: a flexible platform for exploring deep-sequencing data. Nucleic Acids Res. 2014;42:W187–191.PubMedPubMed CentralView ArticleGoogle Scholar
- Phanstiel DH, Boyle AP, Araya CL, Snyder MP. Sushi.R: flexible, quantitative and integrative genomic visualizations for publication-quality multi-panel figures. Bioinformatics. 2014;30:2808–10.PubMedPubMed CentralView ArticleGoogle Scholar
- Zhang H, Meltzer P, Davis S. RCircos: an R package for Circos 2D track plots. BMC Bioinf. 2013;14:244.View ArticleGoogle Scholar
- Csardi G, Nepusz T. The igraph software package for complex network research. Inter J Complex Syst. 2006;1695:1–9.Google Scholar