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Erratum to: Quartz-Seq: a highly reproducible and sensitive single-cell RNA sequencing method, reveals non-genetic gene-expression heterogeneity
Genome Biology volume 18, Article number: 9 (2017)
After publication of our article , we noticed some errors. In this manuscript, we amplified cDNA with 41.67 nmol/l RT primer and 70 nmol/l tagging primer for single-cell Quartz-Seq, and not pmol/l as originally stated. In addition, two mathematical expressions were inadvertently omitted.
Thus, in the section Whole-transcript amplification for single-cell Quartz-Seq, the following are correct:
Immediately after the second centrifugation, 0.8 μl of priming buffer (1.5× PCR buffer with MgCl2 (TaKaRa Bio), 41.67 nmol/l of the RT primer (HPLC-purified; Table 1), 4 U/μl of RNase inhibitor (RNasin Plus; Promega Corp., Madison, WI, USA), and 50 μmol/l dNTPs were added to each tube.
We then added 23 μl of the second-strand buffer (1.09× MightyAmp Buffer v2 (TaKaRa), 70 nmol/l tagging primer (HPLC-purified; Table 1), and 0.054 U/μl MightyAmp DNA polymerase (TaKaRa)) to each tube.
In the section Bioinformatics analysis, the equations should appear as follows:
The MI is considered the Kullback–Leibler distance from the joint probability density to the product of the marginal probability densities as follows:
The MI is always non-negative, symmetric, and equal to 0 only if × and Y are independent. The MI can be represented as a summation of entropies:
Sasagawa Y, Nikaido I, Hayashi T, Danno H, Uno KD, Imai T, et al. Quartz-Seq: a highly reproducible and sensitive single-cell RNA sequencing method, reveals non-genetic gene-expression heterogeneity. Genome Biol. 2013;14:R31.
The online version of the original article can be found under doi:10.1186/gb-2013-14-4-r31.
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Sasagawa, Y., Nikaido, I., Hayashi, T. et al. Erratum to: Quartz-Seq: a highly reproducible and sensitive single-cell RNA sequencing method, reveals non-genetic gene-expression heterogeneity. Genome Biol 18, 9 (2017). https://doi.org/10.1186/s13059-017-1154-x