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  • Erratum
  • Open Access

Erratum to: Quartz-Seq: a highly reproducible and sensitive single-cell RNA sequencing method, reveals non-genetic gene-expression heterogeneity

  • 1, 7,
  • 1, 7,
  • 2,
  • 3,
  • 1,
  • 4, 5 and
  • 1, 3, 6Email author
Contributed equally
Genome Biology201718:9

https://doi.org/10.1186/s13059-017-1154-x

  • Received: 12 January 2017
  • Accepted: 12 January 2017
  • Published:

The original article was published in Genome Biology 2013 14:3097

Erratum

After publication of our article [1], we noticed some errors. In this manuscript, we amplified cDNA with 41.67 nmol/l RT primer and 70 nmol/l tagging primer for single-cell Quartz-Seq, and not pmol/l as originally stated. In addition, two mathematical expressions were inadvertently omitted.

Thus, in the section Whole-transcript amplification for single-cell Quartz-Seq, the following are correct:

Immediately after the second centrifugation, 0.8 μl of priming buffer (1.5× PCR buffer with MgCl2 (TaKaRa Bio), 41.67 nmol/l of the RT primer (HPLC-purified; Table 1), 4 U/μl of RNase inhibitor (RNasin Plus; Promega Corp., Madison, WI, USA), and 50 μmol/l dNTPs were added to each tube.

And

We then added 23 μl of the second-strand buffer (1.09× MightyAmp Buffer v2 (TaKaRa), 70 nmol/l tagging primer (HPLC-purified; Table 1), and 0.054 U/μl MightyAmp DNA polymerase (TaKaRa)) to each tube.

In the section Bioinformatics analysis, the equations should appear as follows:

The MI is considered the Kullback–Leibler distance from the joint probability density to the product of the marginal probability densities as follows:
$$ MI\left(X,Y\right)={E}_{f\left(x,y\right)}\left\{ \log \frac{f\left(x,y\right)}{f(x)f(y)}\right\} $$
(1)
The MI is always non-negative, symmetric, and equal to 0 only if × and Y are independent. The MI can be represented as a summation of entropies:
$$ MI\left(X,Y\right)=H(X)+H(Y)-H\left(X,Y\right) $$
(2)

Notes

Declarations

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Authors’ Affiliations

(1)
Functional Genomics Unit, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-minamimachi, Chuo-ku, 650-0047 Kobe, Hyogo, Japan
(2)
Genome Resource and Analysis Unit, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-minamimachi, Chuo-ku, 650-0047 Kobe, Hyogo, Japan
(3)
Laboratory for Systems biology, RIKEN Center for Developmental Biology, 2- 2-3 Minatojima-minamimachi, Chuo-ku, 650-0047 Kobe, Hyogo, Japan
(4)
Laboratory for Sensory Circuit Formation, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-minamimachi, Chuo-ku, 650-0047 Kobe, Hyogo, Japan
(5)
JST, PRESTO, 2-2-3 Minatojima-minamimachi, Chuo-ku, 650-0047 Kobe, Hyogo, Japan
(6)
Laboratory for Synthetic Biology, Quantitative Biology Center, RIKEN, 2-2-3 Minatojima-minamimachi, Chuo-ku, 650-0047 Kobe, Hyogo, Japan
(7)
Bioinformatics Research Unit, Advanced Center for Computing and Communication, RIKEN, 2-1 Hirosawa, 351-0198 Wako, Saitama, Japan

Reference

  1. Sasagawa Y, Nikaido I, Hayashi T, Danno H, Uno KD, Imai T, et al. Quartz-Seq: a highly reproducible and sensitive single-cell RNA sequencing method, reveals non-genetic gene-expression heterogeneity. Genome Biol. 2013;14:R31.View ArticlePubMedPubMed CentralGoogle Scholar

Copyright

© The Author(s). 2017

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