Fig. 5
From: Dietary nitrogen alters codon bias and genome composition in parasitic microorganisms
Model for synonymous codon use for Mollicutes explains relative synonymous codon use with ~94 % accuracy and recapitulates the difference in the nitrogen cost of genes. a The average mRNA nitrogen content per codon for 168 orthologous genes in the Mollicutes. b The average nitrogen content per double-stranded codon (dsDNA) for the genes in a. c Empirical codon use probabilities (expressed as percentages) plotted against themselves. d The average mRNA nitrogen content per codon for sequences simulated using synonymous codon use probabilities derived from fitting the selection-mutation model to the observed sequence data. e The average nitrogen content per double-stranded codon (dsDNA) for the genes in d. f The synonymous codon use probabilities inferred using the selection-mutation model plotted against the empirical codon use probabilities expressed as percentages. Dot colour corresponds to codon use probabilities for each nitrogen availability group. LN R2 = 0.95, MN R2 = 0.97, HN R2 = 0.91