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Fig. 3 | Genome Biology

Fig. 3

From: Full-length autonomous transposable elements are preferentially targeted by expression-dependent forms of RNA-directed DNA methylation

Fig. 3

Single-locus characterization of the DCL3-RdDM pathway. a CHH methylation of the AtCopia68 family fragmented TE At5TE76210 (blue box), which is located in the second intron of the Agenet domain gene At5G52070. The CHH methylation pathway targeting this TE fragment is additionally responsible for the CHH methylation of the adjacent genic introns and exon (red box), which contain small inverted repeat (IR) and palindromic sequences. b Quantification of perfectly and uniquely mapping small RNAs generated from the single copy genic region from part A (red box) that is targeted by DCL3-RdDM. c Single-locus bisulfite sequencing of biological replicate samples for the region of the At5G52070 gene in the red box in (a). d Single-locus bisulfite sequencing of biological replicate samples for the DCL3-RdDM target TE At3TE40740 (AtSINE4). Error bars in (c) and (d) indicate the 95 % confidence interval. Coloring of genotype labels is the same as in Fig. 2

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Genome Biology

ISSN: 1474-760X

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