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Chloroplast genomes: diversity, evolution, and applications in genetic engineering

Genome Biology volume 17, Article number: 134 (2016) Cite this article

Abstract

Chloroplasts play a crucial role in sustaining life on earth. The availability of over 800 sequenced chloroplast genomes from a variety of land plants has enhanced our understanding of chloroplast biology, intracellular gene transfer, conservation, diversity, and the genetic basis by which chloroplast transgenes can be engineered to enhance plant agronomic traits or to produce high-value agricultural or biomedical products. In this review, we discuss the impact of chloroplast genome sequences on understanding the origins of economically important cultivated species and changes that have taken place during domestication. We also discuss the potential biotechnological applications of chloroplast genomes.

Introduction

Chloroplasts are active metabolic centers that sustain life on earth by converting solar energy to carbohydrates through the process of photosynthesis and oxygen release. Although photosynthesis is often recognized as the key function of plastids, they also play vital roles in other aspects of plant physiology and development, including the synthesis of amino acids, nucleotides, fatty acids, phytohormones, vitamins and a plethora of metabolites, and the assimilation of sulfur and nitrogen. Metabolites that are synthesized in chloroplasts are important for plant interactions with their environment (responses to heat, drought, salt, light, and so on) and their defense against invading pathogens. So, chloroplasts serve as metabolic centers in cellular reactions to signals and respond via retrograde signaling [1, 2]. The chloroplast genome encodes many key proteins that are involved in photosynthesis and other metabolic processes.

The advent of high-throughput sequencing technologies has facilitated rapid progress in the field of chloroplast genetics and genomics. Since the first chloroplast genome, from tobacco (Nicotiana tabacum), was sequenced in 1986 [3], over 800 complete chloroplast genome sequences have been made available in the National Center for Biotechnology Information (NCBI) organelle genome database, including 300 from crop and tree genomes. Insights gained from complete chloroplast genome sequences have enhanced our understanding of plant biology and diversity; chloroplast genomes have made significant contributions to phylogenetic studies of several plant families and to resolving evolutionary relationships within phylogenetic clades. In addition, chloroplast genome sequences have revealed considerable variation within and between plant species in terms of both sequence and structural variation. This information has been especially valuable for our understanding of the climatic adaptation of economically important crops, facilitating the breeding of closely related species and the identification and conservation of valuable traits [4, 5]. Improved understanding of variation among chloroplast genomes has also allowed the identification of specific examples of chloroplast gene transfer to plant nuclear or mitochondrial genomes, which has shed new light on the relationship between these three genomes in plants.

In addition to improving our understanding of plant biology and evolution, chloroplast genomics research has important translational applications, such as conferring protection against biotic or abiotic stress and the development of vaccines and biopharmaceuticals in edible crop plants. Indeed, the first commercial-scale production of a human blood protein in a Current Good Manufacturing Processes (cGMP) facility was published recently [6]. The lack of conservation of intergenic spacer regions, even among chloroplast genomes of closely related plant species, and the species specificity of regulatory sequences have facilitated the development of highly efficient transformation vectors for the integration and expression of foreign genes in chloroplasts. Because the published literature is rarely cross-referenced, this review highlights the impact of chloroplast genomes on various biotechnology applications. In addition to our enhanced understanding of chloroplast biology, we discuss in depth the roles of chloroplast genome sequences in improving our understanding of intracellular gene transfer, conservation, diversity, and the genetic basis by which chloroplast transgenes are engineered to enhance plant agronomic traits or to produce high-value agricultural or biomedical products. In addition, we discuss the impact of chloroplast genome sequences on increasing our understanding of the origins of economically important cultivated species and changes that occurred during domestication.

Advances in chloroplast genome sequencing technology

One of the important factors in the rapid advancement of the chloroplast genomics field is improvement in sequencing technologies. In studies conducted before the availability of high-throughput methods, isolated chloroplasts were used for the amplification of the entire chloroplast genome by rolling circle amplification [7–12]. An alternative strategy is to screen bacterial artificial chromosome (BAC) or fosmid libraries using chloroplast genome sequences as probes [13–20]; however, these methods are subject to many challenges, including difficulty in constructing good-quality BAC or fosmid libraries, large numbers of PCR reactions, and the possibility of contamination from other organellar DNA [21–32]. The PCR approach is also difficult to apply to species that have no relatives whose chloroplast genomes have been sequenced or those with highly rearranged chloroplast genomes.

The development of next-generation sequencing (NGS) methods provided scientists with faster and cheaper methods to sequence chloroplast genomes. Moore and colleagues [33] first reported using NGS to determine chloroplast genome sequences, in Nandina and Platanus. Although multiple NGS platforms are available for chloroplast genome sequencing [34], Illumina is currently the major NGS platform used for chloroplast genomes [21, 32, 35, 36] because it allows the use of rolling circle amplification products [35, 37]. Investigators can then use bioinformatics platforms to perform de novo assembly without the need for reference genome sequences; from these assemblies it is possible to identify consensus chloroplast genome sequences [32]. A third-generation sequencer, the PacBio system which uses single molecule real-time (SMRT) sequencing, is now widely used in chloroplast genome sequencing [38–43]. Its advantage is long read lengths [44], which facilitate de novo genome assembly, particularly in the four chloroplast junctions between the inverted repeat (IR) and single-copy regions.

The low accuracy (~85 % of the raw data) of the long reads produced by the PacBio platform [45] can be corrected by combining the latest chemistry with a hierarchical genome assembly process algorithm; accuracy rates as high as 99.999 % can be achieved after such post-error corrections [46]. Accuracy can also be increased using Illumina short reads [42]. In a study of Potentilla micrantha, sequencing with the Illumina platform produced seven contigs covering only 90.59 % of the chloroplast genome; by contrast, using the PacBio platform with error correction, the entire genome was successfully assembled in a single contig [39].

Chloroplast genome structure

The chloroplast genomes of land plants have highly conserved structures and organization of content; they comprise a single circular molecule with a quadripartite structure that includes two copies of an IR region that separate large and small single-copy (LSC and SSC) regions (Fig. 1a, b). The chloroplast genome includes 120–130 genes, primarily participating in photosynthesis, transcription, and translation. Recent studies have identified considerable diversity within non-coding intergenic spacer regions, which often include important regulatory sequences [13]. Despite the overall conservation in structure, chloroplast genome size varies between species, ranging from 107 kb (Cathaya argyrophylla) to 218 kb (Pelargonium), and is independent of nuclear genome size (Table 1). Certain lineages of land-plant chloroplast genomes also show significant structural rearrangements, with evidence of the loss of IR regions or entire gene families. Furthermore, there is also evidence for the existence of linear chloroplast genomes, as illustrated in Fig. 1b. The percentage of each form within the cell varies in different reports [47, 48].

Fig. 1
figure1

Map of the soybean (Glycine max) chloroplast genome. This genome was used to engineer biotic stress tolerance against insects and herbicides. The quadripartite structure includes two copies of an IR region (IRA and IRB) that separate large single-copy (LSC) and small single-copy (SSC) regions [18]. a Circular form. The GC content graph (gray circle inside) marks the 50 % threshold of GC content. b Linear form. Different colors indicate genes in different functional groups. IR inverted repeat, LSU large subunit, SSU small subunit

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Table 1 Alphabetical list of crop and tree species that have complete annotated chloroplast genome sequences
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Like the genes, the introns in land-plant chloroplast genomes are generally conserved, but the loss of introns within protein-coding genes has been reported in several plant species [49], including barley (Hordeum vulgare) [8], bamboo (Bambusa sp.) [28], cassava (Manihot esculenta) [20], and chickpea (Cicer arietinum) [7]. The proteins encoded by genes in which intron loss is known to occur have diverse functions; they include an ATP synthase (atpF), a Clp protease (clpP), an RNA polymerase (rpoC2), and ribosomal proteins (rpl2, rps12, and rps16) [49]. The majority of reported intron losses have been observed in specific plant groups or species, although some examples of intron loss (such as that in clpP) occur in diverse plant species, including monocots (Poaceae), eudicots (Onagraceae and Oleaceae) and gymnosperms (Pinus) [49].

Diversity of chloroplast genome sequences

At higher taxonomic levels (family level), protein-coding regions and conserved sequences of the chloroplast genome can be used for phylogenetic analysis and domestication studies [49]. Earlier phylogenetic analyses utilized partial chloroplast DNA sequences. The use of variable regions or multiple DNA fragments dramatically enhanced the utility of these analyses but there is insufficient information in these sequences to provide the high-resolution necessary to differentiate closely related taxa, particularly some within-species taxa whose taxonomic relationships are unclear. Complete chloroplast genome sequences are valuable for deciphering phylogenetic relationships between closely related taxa and for improving our understanding of the evolution of plant species.

In this section, we discuss several examples of comparisons of chloroplast genomes, within and between crop species, that have provided unique insight into evolutionary relationships among taxa. We also discuss the origin and geographic distribution of economically important species, as well as their adaptations to different climatic conditions and the use of genome information in their breeding and conservation.

A key application of the chloroplast genome in agriculture is the identification of commercial cultivars and the determination of their purity. DNA barcodes derived from the chloroplast genome can be used to identify varieties and in the conservation of breeding resources. Success in breeding is determined by genetic compatibility and chloroplast genomes serve as a valuable tool for identifying plants that are likely to be closely related and, therefore, genetically compatible. Understanding the genetic relationships between cultivated crops and their wild relatives informs efforts to introduce specific advantageous traits into cultivated crops. In the section below, we discuss how chloroplast genomes have been used to elucidate the evolutionary relationships and domestication history of a few major crops and how this informs breeding programs.

Breeding

The Orchidaceae is a large family that encompasses about 6–11 % of all angiosperms [50] and is important in floriculture. Many commercially important orchid species belong to the subfamily Epidendroideae and chloroplast genomes of several species from this subfamily have been sequenced [51–58]. Because it is easy to perform inter-generic crossing in orchids and because the record of breeding is sometimes incomplete, it is often difficult to validate the parental origin of commercially important varieties [54]. Corrected parental information is important for breeding and variety identification. In an investigation of the Oncidiinae, a subtribe within the Epidendroideae, PCR products derived from eight conserved regions in 15 commercial varieties resolved their phylogenetic relationship at the species level [54] and helped to resolve putative errors in parental origin. Parental records had indicated that Odontoglossum ‘Violetta von Holm’, Odontoglossum ‘Margarete Holm’ and Odontocidium ‘Golden Gate’ are derived from the same female parent (Odontoglossum bictoniense) but phylogenetic analyses of ‘Violetta von Holm’ did not correlate with those of ‘Golden Gate’ or ‘Margarete Holm’ [54]. A possible reason for inconsistencies between the chloroplast DNA-based phylogenetic tree and the parental record is chloroplast capture. Chloroplast capture is the introgression of chloroplasts from one species into another after intrageneric and intergeneric hybridization [59]. Although chloroplast genomes provide useful information for phylogenetic analyses involving closely related taxa, chloroplast capture by hybridization may distort phylogenetic relationships if captured chloroplast genomes or genes included therein are used [60]. The use of both nuclear and chloroplast genomes can provide more complete phylogenies [4, 61].

Phylogenetic studies

There are several published chloroplast genomes from cereals, including those from sorghum (Sorghum bicolor), barley [8], maize (Zea mays) [62], wheat (Triticum aestivum) [63], rye (Secale cereale) [64], and rice (Oryza sativa) [65]. Rice is one of the world's most important crops and is the primary carbohydrate source for the global human population (http://www.ers.usda.gov/topics/crops/rice.aspx). The Oryza species are classified into ten genome types, including six diploids (AA, BB, CC, EE, FF, and GG) and four allotetraploids (BBCC, CCDD, HHJJ, and HHKK). Attempts to clarify the evolutionary relationships between cultivated rice and its wild relatives remain contentious and inconclusive [4]. For example, there are two wild species that have an AA genome in Australia, Oryza meridionalis (annual) and Oryza rufipogon (perennial). Oryza sativa was domesticated from Asian O. rufipogon 10,000 years ago [65]. Nevertheless, analysis of complete Australian and Asian wild rice chloroplast genomes indicated that Australian O. rufipogon chloroplast genomes are more similar to those of Australian O. meridionalis than to those of Asian O. rufipogon [65–67]. Using 19 chloroplast genomes of Oryza AA genome species, a robust phylogenetic tree was established, which will aid in improving rice crops and in conservation strategies [4, 5].

Cotton is the most important textile fiber crop and the first cotton (Gossypium hirsutum) chloroplast genome was published in 2006 [11]. The diploid Gossypium species comprise eight genome groups (A to G and K genomes). Gossypium hirsutum (upland cotton), the most widely planted cotton species in the world, is an allotetraploid of the ancestral A and D genome species [68]. Chloroplast genome sequences are available for 22 Gossypium species and these can be used to glean information about the evolution and domestication of this crop [11, 68, 69] (Table 1). Simple sequence repeat primers were used to investigate 41 species of Gossypium, including all eight genome groups and allotetraploid species [70]. The results indicated that two modern A-genome species, Gossypium herbaceum and Gossypium arboretum, were not cytoplasmic donors of tetraploid (AD) species; instead, the AD genome species originated from an extinct ancestor species of the modern A genome [68, 70].

Domestication

Information on chloroplast genomes is useful for understanding the domestication of several crops, particularly legumes [71]. The chloroplast genome structure of legumes is very interesting; it contains multiple rearrangements, including large inverted segments and loss of inverted repeats [72]. An example is a 51-kb inversion that was first identified in the soybean (Glycine max) chloroplast genome sequence [18] before being reported in most members of the subfamily Papilionoideae [7, 73–77]. A 78-kb reversion was subsequently confirmed in Phaseolus and Vigna chloroplast genomes [78, 79]. More recently, 36-kb [80] and 5.6-kb [81] inversions inside the 51-kb inversion were identified. There are many important genes within these inverted regions but no gene is disturbed and plant survival and performance are not affected. These unique characteristics are not only very useful in phylogenetic studies [82] but also provide important information for chloroplast transformation in legumes. Chloroplast structure is also important for the design of primers needed in the amplification of sequences for further domestication and phylogenetic analysis.

Citrus is one of the most commercially important fruit genera. In 2006, the first Citrus chloroplast genome, that of sweet orange (Citrus × sinensis), was published [12] and this served as a reference genome for subsequent publications [83, 84]. Phylogenetic analysis of 34 chloroplast genomes of Citrus (28) and Citrus-related genera (6) indicated that citrus fruits have the same common ancestor [84, 85]. In four genes (matK, ndhF, ycf1, and ccsA), single-nucleotide variations and insertion/deletion frequencies were clearly higher than average and showed that these genes have been positively selected. The matK gene encodes a maturase that is involved in splicing type II introns and the matK sequence is often used in phylogenetic and evolutionary studies [84]. Positive selection of matK is observed not only in citrus but is common in several other plant species. In fact, more than 30 plant groups have been shown to undergo positive selection of matK genes, indicating that the gene is subject to a number of different ecological selective pressures [86]. The ndhF gene encodes a subunit of the chloroplast NAD(P)H dehydrogenase (NDH) complex. Chloroplast NDH monomers are sensitive to high light stress, suggesting that the ndh genes may also be involved in stress acclimation [87]. These studies indicated that matK and ndhF show positive selection in Australian species, potentially contributing to their adaptation to a hot, dry climate [84, 85].

Bamboo is an economically and ecologically important forest plant in Asia [88]. Bamboo grows quickly and new culms are regenerated from the rhizome after harvesting, making it a sustainable and ecologically and environmentally friendly crop. The first two bamboo chloroplast genomes have been published [28] and many more bamboo chloroplast genomes are now available [88–93]. Bamboo has a long juvenility and it is difficult to obtain flowers for taxonomic studies; consequently the taxonomic relationships of bamboo have proven challenging to unravel on the basis of traditional reproductive organ morphology. Furthermore, the extremely low rate of sequence divergence meant that the taxonomic and phylogenetic relationships of temperate woody bamboos at lower taxonomic levels proved difficult to resolve [88]. These relationships were eventually resolved with high-resolution phylogenetic trees using 25 bamboo chloroplast genomes [93]. In addition to woody bamboos, chloroplast genomes have also been published for herbaceous bamboo [88, 92]. An interesting phenomenon identified in herbaceous bamboo chloroplast genomes is that of gene transfer from the mitochondrial genome to the chloroplast genome. This was an unusual observation, as the chloroplast genome is thought to be nearly immune to the transfer of DNA from nuclear and mitochondrial genomes [88, 92, 94]. A possible reason for this recalcitrance to DNA transfer is the lack of an efficient DNA uptake system [94]. Prior to its observation in herbaceous bamboo, this phenomenon was only observed in two eudicot chloroplast genomes [94] and in monocots [88, 92].

Transfer of chloroplast genes to nuclear or mitochondrial genomes

There are three distinct genomes in plant cells: nuclear, mitochondrial, and plastid. Mitochondria are believed to have evolved from a single endosymbiotic event by the uptake of a proteobacterium, whereas chloroplasts evolved from endosymbiosis of a cyanobacterium, after which there was a massive transfer of genes from the chloroplast to the nucleus [95]. There are distinct translation systems in these organelles: nuclear-encoded genes are translated in the cytosol and the protein products are then transported to the locations in which they function, including chloroplasts [96], whereas chloroplast-encoded proteins are directly synthesized within the chloroplast. Multi-subunit functional protein complexes that are involved in photosynthesis or protein synthesis are also assembled within chloroplasts.

Gene content, number, and structure are conserved in the chloroplast genome sequences of most autotrophic land plants [97, 98] but some protein-encoding genes are absent in specific species [49]. The loss of genes such as infA, rpl22, and ndh from the chloroplast genome and their intracellular transfer to the nuclear or mitochondrial genomes provide valuable information for phylogenetic analyses and evolutionary studies. It is very easy to identify the chloroplast origin of genes in plant mitochondrial or nuclear genomes [99, 100] by intracellular gene transfer [32], but this could also lead to erroneous phylogenic relationships when short sequences are used instead of complete chloroplast genome sequences.

The chloroplast translation initiation factor 1 (infA) is a homolog of the essential gene infA in Escherichia coli [101, 102]. This gene initiates translation in collaboration with two nuclear-encoded initiation factors to mediate interactions between mRNA, ribosomes, and initiator tRNA-Met [102]. Many parallel losses of chloroplast-encoded infA have occurred during angiosperm evolution [102] (Fig. 2). Nuclear-encoded infA genes have been identified in Arabidopsis thaliana, soybean, tomato (Solanum lycopersicum), and ice plant (Mesembryanthemum crystallinum) [102]. Protein sequences of nuclear-encoded infA in these four species contain chloroplast transit peptides. Studies using soybean and A. thaliana infA-GFP proteins have shown that nuclear-encoded infA genes are translated in the cytosol and transported into chloroplasts [102]. Many more chloroplast-encoded infA deletions have been identified recently (Fig. 2).

Fig. 2
figure2

Chloroplast genome structure and gene expression across tracheophytes. These 658 chloroplast genomes were downloaded from NCBI Organelle Resources. The X-axis indicates the taxonomy of the chloroplast genome species following the Angiosperm Phylogeny Group III system and NCBI taxonomy. The bar width represents 100 species. The Y-axis shows the chloroplast genes, which were classified by different chloroplast regions. Gray boxes indicate absence of genes. Red boxes indicate stop codons in genes. Blue boxes indicate unknown nucleotides (N) in genes. IR inverted repeat, LSC large single-copy region, SSC small single-copy region

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There are 57 chloroplast genomes in 26 genera in which the essential gene rpl22 is reported to have been deleted from the chloroplast and transferred to the nuclear genome (Fig. 2) [14, 103]. Nuclear-encoded rpl22 contains a transit peptide that is predicted to deliver this protein from the cytosol to chloroplasts. These peptides are diverse, suggesting that there were two independent rpl22 transfers in the Fabaceae and the Fagaceae [14]. Similar transfer to the nucleus has also been observed for rpl32 deletion from chloroplast genomes [104–106].

Eleven chloroplast genes encode ndh subunits, which are involved in photosynthesis. The ndh proteins assemble into the photosystem I complex to mediate cyclic electron transport in chloroplasts [107, 108] and facilitate chlororespiration [109]. Some autotrophic plants lack functional ndh genes in their chloroplast genomes [36, 51, 54, 55, 110–115] (Fig. 2). Unlike the single gene losses described previously, the entire family of ndh genes has been deleted in these plants. Seven orchid chloroplast genomes indicated at least three independent ndh deletions [32]. Some orchid ndh DNA fragments were identified in the mitochondrial genome but the complete ndh genes required to translate putative functional protein complexes are absent [32]. In the nuclear genome of Norway spruce, only non-functional plastid ndh gene fragments are present [116]. Normal photosynthesis is observed in these ndh-deleted species [32, 117]. Furthermore, ndh-deleted transformants are autotrophic and produce carbohydrates through photosynthesis [107, 118–121].

Many more chloroplast-gene deletions have been observed, including deletions of accD, ycf1, ycf2, ycf4, psaI, rpoA, rpl20, rpl23, rpl33, and rps16; many unique gene deletions have been identified in only one or a few species (psbJ, rps2, rps14, and rps19) (Fig. 2). The functions of these genes, phenotypes of their knock-out mutants, and evidence for their transfer are summarized in Additional file 1. Most essential genes that have been lost from chloroplast genomes have been transferred to the nucleus to maintain the plant's photosynthetic capacity, with the exception of ycf1 and ycf2.

In summary, chloroplast genome sequences are most valuable for understanding plant evolution and phylogeny. Databases of not only plant genomes but also plant transcriptomes will be useful in investigating deletion events or the transfer of chloroplast genes to other organellar genomes to complement such deletions.

Advances in chloroplast genome engineering

In the past century, desirable agronomic traits, including yield enhancement and resistance to pathogens or abiotic stress, were achieved by breeding cultivated crops with their wild relatives. As explained above, chloroplast genome sequences are very useful in the identification of closely related, breeding-compatible plant species. With the advent of modern biotechnology, desirable traits from unrelated species can now be readily introduced into commercial cultivars. Such genetically modified crops have revolutionized agriculture in the past two decades, dramatically reducing the use of chemical pesticides and herbicides while enhancing yield. For most commercial cultivars, herbicide- or insect-resistance genes are introduced into the nuclear genome. There are, however, a few limitations for nuclear transgenic plants, including low levels of expression (<1 % total soluble protein (TSP)) and potential escape of transgenes via pollen.

Engineering the introduction of foreign genes into the chloroplast genome addresses both of these concerns. Just two copies of transgenes are typically introduced into the nuclear genome, whereas up to 10,000 transgene copies have been engineered into the chloroplast genome of each plant cell, resulting in extremely high levels of foreign gene expression (>70 % TSP) [122]. Most importantly, chloroplast genomes are maternally inherited in most cultivated crops, minimizing or eliminating transgene escape via pollen [123].

The basic process of chloroplast engineering is explained in Fig. 3a, b. Chloroplast genome engineering is accomplished by integrating foreign genes into intergenic spacer regions without disrupting the native chloroplast genes (Fig. 3a). Two chloroplast genes are used as flanking sequences to facilitate integration of transgene cassettes. Transgene cassettes include a selectable marker gene and gene(s) of interest, both regulated by chloroplast gene promoters and untranslated regions (UTRs; Fig. 3a). Chloroplast genome sequences are essential to build transgene cassettes because they provide both flanking and regulatory sequences. Transgene cassettes that are inserted into bacterial plasmids are called chloroplast vectors and they are bombarded into plant cells using gold particles and a gene gun (Fig. 3b). Because of the presence of chloroplast DNA in the nuclear or mitochondrial genome, transgene cassettes may integrate via homologous or non-homologous recombination events; but any transgenes that are integrated within the nuclear or mitochondrial genome will not be expressed because chloroplast regulatory sequences are not functional in other genomes. If such integration occurs, the transgenes could be easily identified by evaluation of their integration site and eliminated [124].

Fig. 3
figure3

Basic process of chloroplast genetic engineering, diversity in intergenic spacer regions, and impact of transgene integration (endogenous versus heterologous genome sequences). a Complexity of heterologous sequence integration into intergenic spacer regions between lettuce and tobacco. The schematic diagram represents recombination between the tobacco transplastomic genome and the lettuce transformation vector [128]. Purple bars represent unique lettuce intron sequence; the green bar represents unique tobacco intron sequence; black bars are exon regions; blue regions are looped out sequence. The expression cassette comprises: promoters (P), leader sequence (L), gene of interest (GOI), terminators (T), and selectable marker gene (SMG). IG intergenic spacer region. b Basic process of chloroplast genetic engineering. Gene delivery is performed by bombardment with gold microparticles coated with chloroplast vectors, followed by three rounds of selection to achieve homoplasmy. After confirmation of transgene integration, plants are grown in the greenhouse to increase biomass. Chloroplast transgenes are maternally inherited without Mendelian segregation of introduced traits. c Comparison of 21 of the most variable intergenic spacer regions among Solanaceae chloroplast genomes. Atr Atropa, Pot potato, Tob tobacco, Tom tomato. *Tier 1, **tier 2, and ***tier 3 regions reported in the paper by Shaw et al. [250]. Plotted values were converted from percentage identity to sequence divergence on a scale from 0 to 1 as shown on the Y-axis; these values demonstrate a wide range of sequence divergence in different regions. Nucleotide sequences were determined by a bridging shotgun method and genome annotation was performed using the Dual Organellar GenoMe Annotator [13]. d, e Decrease in the expression of transgenes regulated by heterologous psbA promoters and untranslated regions (UTRs) engineered via tobacco chloroplast genomes. When the lettuce (La) psbA regulatory region was used in tobacco (Na) chloroplasts or vice versa, transgene expression is dramatically reduced. d Accumulation of a cholera toxin B subunit (CTB) and proinsulin (Pins) fusion protein (CP) was quantified by densitometry and e anthrax protective antigen (PA) accumulation was estimated by enzyme-linked immunosorbent assay (ELISA). Total leaf protein (TLP) or total soluble protein (TSP) data are presented as a function of light exposure and developmental stage. The order of young, mature, and old is different in d and e because of the accumulation of more CTB-Pins in older leaves and PA in mature leaves [128]. Young (top five), mature (fully grown), and old (bottom three) leaves were fully expanded and were cut from plants grown in the greenhouse for 8–10 weeks

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One of the challenges of creating chloroplast transgenic (transplastomic) plants is the elimination of all untransformed copies (>10,000 per cell) of the native chloroplast genome and replacing them with transformed genomes that contain integrated transgene cassettes. The absence of the native chloroplast genome and the presence of only the modified genomes is referred to as the homoplasmic state, which is typically achieved after two or three rounds of selection (Fig. 3b). The most effective selectable marker used is the aadA gene, which confers resistance to streptomycin and spectinomycin. These antibiotics bind specifically to chloroplast ribosomes and disrupt protein synthesis without interfering with any other cellular process. Efforts to transform the chloroplast genome of cereal crops have been mostly unsuccessful. This could be due to the instability of chloroplast DNA in the mature leaves of cereals [47] or to a requirement for better selectable markers [125].

Table 2 provides the first global, comprehensive summary of the power of chloroplast genetic engineering, utilizing valuable information generated by the sequencing of chloroplast genomes described in previous sections. This table includes the most complete list of chloroplast genomes that have been engineered for enhanced agronomic traits or the production of different bio-products, including biopolymers, industrial enzymes, biopharmaceuticals, and vaccines. Within Table 2, transgenes are grouped according to their functions and are organized according to their site of integration. The efficiency of transgene expression is also included in Table 2, providing important information about the regulatory sequences used to express the transgenes.

Table 2 Engineering the chloroplast genome for biotechnology applications
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Impact of sequence diversity in the chloroplast genome on transgene integration

Figure 3a shows examples of transplastomic genomes that have been transformed with either an endogenous or a heterologous flanking sequence. Every single nucleotide change in the heterologous sequence was subsequently edited out and corrected to achieve 100 % homology to the native sequence within the intergenic spacer region (Fig. 3a). The repetitive editing process significantly reduces the efficiency of transgene integration when using heterologous flanking sequences. This challenge is made even more difficult by inadequate conservation of intergenic spacer regions, even within the same family. Figure 3c shows comparisons of 21 of the most variable intergenic spacer regions; only four of the >150 spacer regions, including the trnl/trnA spacer region, are conserved among members of the Solanaceae. Among grass chloroplast genomes, not a single intergenic spacer region is conserved [8]. This necessitates construction of species-specific chloroplast vectors using endogenous flanking sequences and underscores the need to sequence the chloroplast genomes of economically important crop species.

Ideal sites in the chloroplast genome for transgene integration

The selection of a suitable intergenic spacer region from among more than 100 sites found in each chloroplast genome is a major concern. Statements on the lack of positional effects in the transplastomic literature are common and are used to contrast chloroplast genetic engineering with nuclear transgene integration, which is often associated with profound differences in the expression of transgenes dependent on their site of integration. Evidence shows, however, that there are also positional effects within the chloroplast genome (Table 2). IR regions are found in duplicate in most chloroplast genomes; therefore, transgenes should be inserted within the IR region instead of the SSC or LSC regions because this should double the copy number of transgenes. Integration of a transgene cassette into one copy of the IR facilitates integration into the other copy, thereby enhancing selection pressure to achieve homoplasmy through this copy correction mechanism, a characteristic feature of the chloroplast genome [126–128]. Therefore, the site of integration plays a crucial role in transgene expression level and in enhancing homoplasmy under selection by antibiotics. Most importantly, in all sequenced chloroplast genomes within a single plant species, the DNA sequence in one copy of the IR is identical to that in the other copy, without any exception (Table 1).

An early controversy in the chloroplast genetic engineering field was the suitability of transcriptionally silent spacer regions, where native genes (for example, rbcL/accD) are located on opposite strands of the chloroplast genome, or transcriptionally active spacer regions, where native genes (for example, trnA/trnI) are located within operons on the same strand. After a herbicide resistance gene was introduced into the transcriptionally active spacer region for the first time [129], most subsequent studies preferentially used this site of integration (Table 2). The integration of transgenes into the transcriptionally active spacer region (trnl/trnA) has led to 25-fold higher expression of transgenes compared with the transcriptionally silent spacer region (rbcl/accD) [130], possibly due to the presence of multiple promoters (heterologous and endogenous) that enhance transcription. Introns present within trnI/trnA genes (used as flanking sequences) also provide efficient processing of native or foreign transcripts. The trnA gene intron includes a chloroplast origin of replication and produces more copies of the template (chloroplast vectors) for integration of the transgene cassette [131]. In fact, among 114 transgenes in different plant species in Table 2, 71 are integrated at the trnA/trnI site of the chloroplast genome, confirming the unique advantages of this site [127, 129, 130].

Role of chloroplast genome regulatory sequences in transgene expression

In addition to the site of integration, regulatory sequences located upstream (promoter, 5′ UTR) and downstream (3′ UTR) of transgenes play a major role in determining their expression level. The psbA regulatory region, first used almost 25 years ago [131], still appears to be the best option for use in an expression cassette, as the psbA gene encodes the most highly translated protein in the chloroplast [132] and it can also mediate light-induced activation of translation [128]. Indeed, almost all highly expressed transgenes (>70 % TSP, >25 % dry weight) utilize the psbA regulatory region; among 114 transgenes expressed via the chloroplast genome, 84 use the psbA regulatory sequence (Table 2). Other endogenous regulatory sequences that are used include rbcL and atpA, which result in lower transgene expression levels than the psbA promoter/5′ UTR.

Using regulatory regions from photosynthetic genes has the advantage of light regulation, making them ideal for transgene expression in photosynthetic organs (leaves; Fig. 3d, e). However, when the lettuce psbA regulatory region was used in tobacco chloroplasts or vice versa, transgene expression was dramatically reduced (Fig. 3d, e) [128]. Nucleotide differences within the psbA 5′ UTR between tobacco and lettuce (Lactuca sativa) resulted in changes that decreased the interaction of RNA-binding proteins and produced variation in the size of the stem, bulge, and terminal loop of the UTR [128]. In addition, most regulatory proteins (including sigma factors that bind to the promoter region) are nuclear encoded and transported to chloroplasts. This underscores a caveat associated with using regulatory sequences for transgene expression: the need to make species-specific chloroplast vectors to accommodate highly specific regulatory region-binding proteins.

Heterologous regulatory sequences are necessary for transgene expression that is independent of cellular control, especially in non-photosynthetic organs such as fruits and edible roots, where chloroplast protein synthesis is poor [133]. A heterologous UTR (T7 gene10) was first evaluated for expression in leaves [127, 134] and was subsequently tested in non-green tissues. When the expression of BETAINE ALDEHYDE DEHYDROGENASE (BADH) was regulated by the T7 gene10 UTR in carrot (Daucus carota) plants, 75 % of the expression level in leaves was observed in non-green edible roots, conferring the highest level of salt tolerance (400 mM NaCl) found in the published literature (Fig. 4i, j) [135]. Although T7 gene10 has been successfully used to engineer salt tolerance in non-green tissues, its expression level is not as high as that of the psbA regulatory sequence in leaves [136]. The only other heterologous UTR that expressed transgenes at high levels is that from the Bacillus thuringiensis (Bt) operon [137]. Use of this operon produced the highest level of insecticidal toxin protein (52 % TLP) ever reported in the published literature [137]. These high levels of toxin accumulation in chloroplasts could result from the combination of high-level expression and protein stability; the Bt protein formed cuboidal crystals within chloroplasts (Fig. 4e) due to co-expression of a chaperone that facilitates folding. When fed, transplastomic leaves, cotton bollworm (Helicoverpa sp.) were killed with a single bite of leaf and insects that had 40,000-fold increased resistance to Bt were also killed (Fig. 4f, g). Nevertheless, expression of this transgene in tomato fruit is very poor [133, 138, 139] and further research is needed to enhance transgene expression in fruits.

Fig. 4
figure4

Engineering the chloroplast genome to confer biotic/abiotic stress tolerance or expression of high-value products. a–d Industrial production of blood clotting factor IX (FIX) bioencapsulated in lettuce plants in a hydroponic cGMP facility. a Biomass production of FIX-expressing plants. b–d Steps in capsule preparation. After harvesting and lyophilization of fresh leaves, freeze-dried FIX-accumulating leaves were powdered and prepared as capsules [6]. e–g Overexpression of the Bt cry2Aa2 operon in chloroplasts leads to the formation of the Bt insecticidal crystal protein. In bioassays with the Helicoverpa zea, f eating the transplastomic leaf kills the caterpillar, while g the control leaf is consumed by the growing caterpillar [137]. h Ultrastructure of the chloroplast envelope membrane of transplastomic γ-tocopherol methyltransferase (γ-TMT) tobacco plants shows the formation of multiple layers of inner envelope membranes as the result of γ-TMT overexpression [153]. i, j Expression of BETAINE ALDEHYDE DEHYDROGENASE (BADH) in carrot plants. i Transgenic carrot plants thrived in soil irrigated with 400 mM sodium chloride, whereas untransformed carrot plants showed retarded growth in the presence of salt. j Carrot roots from transplastomic plants [135]. k Phenotypes of tomato fruits from transplastomic tomato plants expressing lycopene β-cyclase transgenes compared with wild-type plants. Fruits were harvested at different ripening stages. Orange color of ripe fruits indicates efficient conversion of red lycopene into orange β-carotene (provitamin A) [154]

Full size image

Engineering the chloroplast genomes for biotechnology applications

Conferring stress tolerance

In the past decade, chloroplast genetic engineering has focused primarily on the overexpression of target genes with the potential to enhance biotic stress tolerance, which is very important for plant protection and yield enhancement. Yield loss due to insect pests can be very serious in many countries. In addition to cotton bollworm resistance conferred by hyper-expression of Bt protein in chloroplasts [137], there are many other striking recent examples of improved biotic stress tolerance. Retrocyclin-101 and Protegrin-1 protect against Erwinia soft rot and tobacco mosaic virus (TMV), which result in yield loss in several cultivated crops [140]. Whitefly and aphid resistance has been accomplished by expressing β-glucosidase [141], which releases insecticidal sugar esters from hormone conjugates. Multiple resistances against aphids, whiteflies, lepidopteran insects, and bacterial and viral pathogens were achieved by expressing the Pinellia ternata agglutinin (PTA) gene in the chloroplast genome [142]. More than 40 transgenes have been stably integrated into and expressed within the chloroplast genome, conferring important agronomic traits, including insect resistance in edible crops cabbage (Brassica oleracea) [143], soybean [144, 145], and eggplant (Solanum melongena) [146].

More recently, scientists have begun to explore new strategies to downregulate specific target genes. One such approach is to express double-stranded RNAs (dsRNAs) within the chloroplast genome and to use RNA interference (RNAi) to confer the desired agronomic traits, mainly resistance to insects that cause severe yield loss. This strategy has been demonstrated by expressing long or short dsRNAs that activate RNAi and disrupt target genes in insects, providing efficient protection against insects without the need for chemical pesticides. One such example is the suppression of three essential proteins required for insect survival—lepidopteran chitin synthase (Chi), cytochrome P450 monooxygenase (P450), and V-ATPase—using dsRNAs in the tobacco chloroplast system [147]. Each dsRNA was expressed independently in chloroplasts and leaves were fed to insects. The transcription level of target genes in Helicoverpa insects decreased to almost undetectable levels in the midgut, resulting in a significant reduction in the net weight of larvae and in pupation rate [147]. Transplastomic potato plants producing β-actin-targeting long dsRNA were lethal to Colorado potato beetle (Leptinotarsa decemlineata) larvae, providing yet another crop protection mechanism [148].

Synthesis of enzymes and biomaterials

In addition to improved resistance against both biotic and abiotic stress, the chloroplast genome has been engineered to produce useful enzymes, biomaterials, and biofuels, or even to enhance biomass. The first report of metabolic engineering using chloroplast genomes produced the highest level of the poly(p-hydroxybenzoic acid (pHBA) polymer (25 % dry weight) in normal healthy plants despite the diversion of a major metabolic intermediate [149]. The first use of plant-derived enzyme cocktails for the production of fermentable sugars from lignocellulosic biomass was accomplished recently [150]. Unlike the single biofuel enzymes previously expressed in chloroplasts, nine different genes from bacteria or fungi were expressed in E. coli or tobacco chloroplasts using a new technique that enabled the insertion of fungal genes with several introns, eliminating the need to prepare cDNA libraries. Industrial fermentation systems are currently limited by high cost and low production capacity; chloroplast-derived enzyme cocktails offer several striking advantages, including significantly reduced cost, improved stability of chloroplast-derived enzymes, and no need for enzyme purification. Interestingly, expression of β-glucosidase released hormones from conjugates, resulting in elevated phytohormone levels and increased biomass [141], an unexpected outcome of enzyme expression.

Enhancing nutrition

Seed oils, such as those from soybean, rapeseed (Brassica napus), and maize, are the major dietary source of vitamin E. They have very low α-tocopherol content but relatively high levels of γ-tocopherol. Only a few seed oils, such as sunflower (Helianthus annuus) seed oil, contain high levels of α-tocopherol, an important precursor of vitamin E [151]. γ-Tocopherol is the biosynthetic precursor of α-tocopherol, suggesting that the α-tocopherol biosynthetic pathway catalyzed by γ-tocopherol methyl transferase (γ-TMT) is the rate-limiting step [152]. Engineering of the γ-tmt gene into the chloroplast genome resulted in the formation of multiple layers of the inner chloroplast envelope (Fig. 4h) due to γ-TMT overexpression, with around tenfold higher conversion of γ-tocopherol to α-tocopherol in seeds [153]. Likewise, introducing lycopene β-cyclase genes into the tomato plastid genome increased the conversion of lycopene into provitamin A (β-carotene), with obvious phenotypic changes (Fig. 4k) [154].

Biopharmaceuticals

At present, protein drugs are extremely expensive; for example, >90 % of the global population cannot afford insulin, a drug needed to treat the global diabetes epidemic. The high cost of protein drugs is due to their production in prohibitively expensive fermentation systems (which cost more than $450–700 million to build depending on their capacity [155, 156]), prohibitively expensive purification from host proteins, the need for refrigerated storage and transport, and the short shelf-life of the final product. Protein drugs made by plant chloroplasts overcome most of these challenges because they do not require expensive fermentation systems and are produced in federal drug administration (FDA)-approved hydroponic greenhouses (Fig. 4a) [157]. Lettuce leaves expressing protein drugs are lyophilized and stored indefinitely at ambient temperature without losing their efficacy (Fig. 4b–d) [6]. The plant cell wall protects protein drugs from acids and enzymes in the stomach because human enzymes do not digest plant cell wall glycans. Human gut microbes, however, have evolved to break down every glycosidic bond in the plant cell wall and therefore release the protein drug into the gut lumen, directing its delivery to the blood or immune system [158, 159].

Oral delivery of several human therapeutic proteins expressed in chloroplasts is highly efficacious in the treatment of several human diseases, including diabetes, cardiovascular disease, pulmonary hypertension, and Alzheimer’s disease. Most proteins were expressed in tobacco chloroplasts for initial evaluation and were subsequently expressed in lettuce chloroplasts for advancing them to the clinic. Oral delivery of exendin-4, which modulates the secretion of insulin in a glucose-dependent manner, lowered glucose in diabetic animals by stimulating the production of insulin in a manner similar to that of the injectable drug [160]. Oral delivery of angiotensin-converting enzyme 2 (ACE2) and angiotensin (Ang) (1–7) significantly improved cardiopulmonary structure and function, decreased elevated right ventricular systolic blood pressure, and improved pulmonary blood flow in animals with induced pulmonary hypertension [161]. Oral delivery of plant cells expressing ACE2 and Ang (1–7) also reduced endotoxin-induced uveitis (EIU) and dramatically decreased cellular infiltration and retinal vasculitis, as well as damage and folding in experimental autoimmune uveoretinitis [158]. It is also possible to orally deliver protein drugs across the blood–brain barrier to the Alzheimer’s brain to remove plaques [162].

The first industrial-scale production of human blood clotting factor in a cGMP facility was reported recently [6] (Fig. 4a–d). In a 1000 ft2 hydroponic cGMP facility, it is possible to produce up to 30,000 doses for a 20-kg pediatric patient. Clotting factor made in lettuce was stable for up to 2 years when lyophilized cells were stored at ambient temperature, completely eliminating the need for the cold chain. This enables the first commercial development of an oral drug and addresses the extremely expensive purification, cold storage and transportation, and short shelf-life of current protein drugs. Oral delivery of a broad dose range was effective in the prevention of antibody formation after injection of clotting factor IX (FIX), further facilitating human clinical studies.

Vaccines against infectious diseases

The current iteration of vaccines, using attenuated bacteria or viruses, offer protection against major infectious diseases but they also present major challenges. For example, the oral polio vaccine that is used around the globe has caused severe polio resulting from mutations and recombination with other viruses [163]. In addition, all current vaccines require cold storage and transportation, making distribution in developing countries a major challenge. Many of these challenges can be overcome by using chloroplasts.

One successful chloroplast-derived vaccine conferred dual immunity against cholera and malaria in animal studies [164]. Cholera is a major disease causing high mortality, with the only licensed vaccine being not only expensive but also limited in its duration of protection. No vaccine is currently available for malaria. The cholera toxin-B subunit (CTB) of Vibrio cholerae was fused to the malarial vaccine antigen apical membrane antigen-1 (AMA1) and merozoite surface protein-1 (MSP1) and expressed in lettuce or tobacco chloroplasts. While no suitable models exist to test human malaria, a cholera toxin challenge using mice immunized with chloroplast-expressed CTB was highly effective and provided the longest duration of protection in the published literature [164]. These early results show that chloroplasts are ideal for producing low-cost booster vaccines against several infectious diseases [165] for which the global population has been primed previously (Table 2), but lack of an oral priming strategy is still a major limitation in this field.

Moving forward

It is amazing that the chloroplast genome can express >120 foreign genes from different organisms, including bacteria, viruses, fungi, animals, and humans. The insertion of commercially useful traits, including herbicide and insect resistance, into soybean resulted in high-level expression and superior transgene containment, with no antibiotic selectable markers; but even so, these lines were not developed commercially. Nevertheless, recurring concerns about insect resistance against biopesticides have resulted in new USDA requirements on planting Bt corn [122], which may eventually require utilization of the transplastomic approach to confer agronomic traits. The nuclear transgenic approach is inadequate to develop products when higher-level transgene expression is a requirement. Thus, chloroplast transformation has a unique advantage in advancing the field of molecular farming for the production of vaccines, biopharmaceuticals, or other bio-products.

Although products with high-level expression have now advanced to the clinic or are in commercial development, a better understanding of chloroplast translation is required to improve several other gene products. The availability of chloroplast genome sequences should help in the development of codon optimization programs using highly expressed chloroplast genes, but among the ~3000 cultivated crops, sequenced chloroplast genomes are available for crops from fewer than 70 genera. Major funding agencies have not supported crop chloroplast genome sequence projects because of the misconception that all chloroplast genomes are similar, as evidenced by the publication of fewer than ten crop chloroplast genome sequences between 1986 and 2004. This review illustrates the importance of sequencing more crop chloroplast genomes for various biotechnology applications. Furthermore, new selectable markers are needed to transform the chloroplast genomes of cereals, which has been elusive for the past two decades.

Chloroplast genome sequences will be valuable assets in herbal medicine. Most medicinal plants are rare species and very little information is available to confirm their identity. DNA barcodes derived from chloroplast genomes will be useful for identifying varieties and resources; this concept is also valuable in the identification of the origin of cultivated crops and their close relatives to enhance breeding or transfer of useful traits. Molecular techniques to sequence the genomes of single chloroplasts could help to eliminate chloroplast-like sequences that are present in the mitochondrial or nuclear genome. The ability to sequence chloroplast genomes using minimal leaf materials could help us to understand variations in different segments of a variegated leaf in horticultural crops. Further, determining complete chloroplast genome sequences from fossils or recently extinct plants could shed more light on chloroplast genome evolution; help us to understand these species’ inadequate fitness to cope with environmental changes; and help us to build new phylogenetic trees. The technology for isolating DNA from fossils is already available [166–168]. All of these goals can be accomplished with less expensive and more accurate genome sequences, utilizing longer read sequencing technology and new bioinformatics tools.

Abbreviations

ACE2, Angiotensin-converting enzyme 2; BAC, bacterial artificial chromosome; Bt, Bacillus thuringiensis; CTB, Cholera toxin B subunit; cGMP, Current Good Manufacturing Processes; CTB, Cholera toxin B subunit; dsRNA, double-stranded RNA; FIX, clotting factor IX; infA, translation initiation factor 1; IR, inverted repeat; LSC, large single copy; ndh, NAD(P)H dehydrogenase; NCBI, National Center for Biotechnology Information; NGS, next-generation sequencing; RNAi, RNA interference; SSC, small single copy; γ-TMT, γ-Tocopherol methyltransferase; TSP, total soluble protein; UTR, untranslated region; Ang (1–7), Angiotensin (1–7)

References

  1. 1.

    Bobik K, Burch-Smith TM. Chloroplast signaling within, between and beyond cells. Front Plant Sci. 2015;6:781.

    PubMed  PubMed Central  Article  Google Scholar 

  2. 2.

    Daniell H, Chan H-T, Pasoreck EK. Vaccination through chloroplast genetics: affordable protein drugs for the prevention and treatment of inherited or infectious human diseases. Annu Rev Genet. 2016. In Press.

  3. 3.

    Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashida N, Matsubayashi T, et al. The complete nucleotide sequence of the tobacco chloroplast genome: its gene organization and expression. EMBO J. 1986;5:2043–9.

    CAS  PubMed  PubMed Central  Google Scholar 

  4. 4.

    Wambugu P, Brozynska M, Furtado A, Waters D, Henry R. Relationships of wild and domesticated rices (Oryza AA genome species) based upon whole chloroplast genome sequences. Sci Rep. 2015;5:13957.

    PubMed  PubMed Central  Article  Google Scholar 

  5. 5.

    Brozynska M, Furtado A, Henry RJ. Genomics of crop wild relatives: expanding the gene pool for crop improvement. Plant Biotechnol J. 2016;14:1070–85.

    CAS  PubMed  Article  Google Scholar 

  6. 6.

    Su J, Zhu L, Sherman A, Wang X, Lin S, Kamesh A, et al. Low cost industrial production of coagulation factor IX bioencapsulated in lettuce cells for oral tolerance induction in hemophilia B. Biomaterials. 2015;70:84–93.

    CAS  PubMed  Article  Google Scholar 

  7. 7.

    Jansen RK, Wojciechowski MF, Sanniyasi E, Lee SB, Daniell H. Complete plastid genome sequence of the chickpea (Cicer arietinum) and the phylogenetic distribution of rps12 and clpP intron losses among legumes (Leguminosae). Mol Phylogenet Evol. 2008;48:1204–17.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  8. 8.

    Saski C, Lee SB, Fjellheim S, Guda C, Jansen RK, Luo H, et al. Complete chloroplast genome sequences of Hordeum vulgare, Sorghum bicolor and Agrostis stolonifera, and comparative analyses with other grass genomes. Theor Appl Genet. 2007;115:571–90.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  9. 9.

    Ruhlman T, Lee SB, Jansen RK, Hostetler JB, Tallon LJ, Town CD, Daniell H. Complete plastid genome sequence of Daucus carota: implications for biotechnology and phylogeny of angiosperms. BMC Genomics. 2006;7:222.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  10. 10.

    Samson N, Bausher MG, Lee SB, Jansen RK, Daniell H. The complete nucleotide sequence of the coffee (Coffea arabica L.) chloroplast genome: organization and implications for biotechnology and phylogenetic relationships amongst angiosperms. Plant Biotechnol J. 2007;5:339–53.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  11. 11.

    Lee SB, Kaittanis C, Jansen RK, Hostetler JB, Tallon LJ, Town CD, Daniell H. The complete chloroplast genome sequence of Gossypium hirsutum: organization and phylogenetic relationships to other angiosperms. BMC Genomics. 2006;7:61.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  12. 12.

    Bausher MG, Singh ND, Lee SB, Jansen RK, Daniell H. The complete chloroplast genome sequence of Citrus sinensis (L.) Osbeck var 'Ridge Pineapple': organization and phylogenetic relationships to other angiosperms. BMC Plant Biol. 2006;6:21.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  13. 13.

    Daniell H, Lee SB, Grevich J, Saski C, Quesada-Vargas T, Guda C, et al. Complete chloroplast genome sequences of Solanum bulbocastanum, Solanum lycopersicum and comparative analyses with other Solanaceae genomes. Theor Appl Genet. 2006;112:1503–18.

    CAS  PubMed  Article  Google Scholar 

  14. 14.

    Jansen RK, Saski C, Lee SB, Hansen AK, Daniell H. Complete plastid genome sequences of three rosids (Castanea, Prunus, Theobroma): evidence for at least two independent transfers of rpl22 to the nucleus. Mol Biol Evol. 2011;28:835–47.

    CAS  PubMed  Article  Google Scholar 

  15. 15.

    Wolfe KH, Morden CW, Palmer JD. Function and evolution of a minimal plastid genome from a nonphotosynthetic parasitic plant. Proc Natl Acad Sci U S A. 1992;89:10648–52.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  16. 16.

    Bortiri E, Coleman-Derr D, Lazo GR, Anderson OD, Gu YQ. The complete chloroplast genome sequence of Brachypodium distachyon: sequence comparison and phylogenetic analysis of eight grass plastomes. BMC Res Notes. 2008;1:61.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  17. 17.

    Sato S, Nakamura Y, Kaneko T, Asamizu E, Tabata S. Complete structure of the chloroplast genome of Arabidopsis thaliana. DNA Res. 1999;6:283–90.

    CAS  PubMed  Article  Google Scholar 

  18. 18.

    Saski C, Lee SB, Daniell H, Wood TC, Tomkins J, Kim HG, Jansen RK. Complete chloroplast genome sequence of Glycine max and comparative analyses with other legume genomes. Plant Mol Biol. 2005;59:309–22.

    CAS  PubMed  Article  Google Scholar 

  19. 19.

    Jansen RK, Kaittanis C, Saski C, Lee SB, Tomkins J, Alverson AJ, Daniell H. Phylogenetic analyses of Vitis (Vitaceae) based on complete chloroplast genome sequences: effects of taxon sampling and phylogenetic methods on resolving relationships among rosids. BMC Evol Biol. 2006;6:32.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  20. 20.

    Daniell H, Wurdack KJ, Kanagaraj A, Lee SB, Saski C, Jansen RK. The complete nucleotide sequence of the cassava (Manihot esculenta) chloroplast genome and the evolution of atpF in Malpighiales: RNA editing and multiple losses of a group II intron. Theor Appl Genet. 2008;116:723–37.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  21. 21.

    Wu J, Liu B, Cheng F, Ramchiary N, Choi SR, Lim YP, Wang X-W. Sequencing of chloroplast genome using whole cellular DNA and Solexa sequencing technology. Front Plant Sci. 2012;3:234.

    Google Scholar 

  22. 22.

    Grivet D, Heinze B, Vendramin G, Petit R. Genome walking with consensus primers: application to the large single copy region of chloroplast DNA. Mol Ecol Notes. 2001;1:345–9.

    CAS  Article  Google Scholar 

  23. 23.

    Goremykin V, Hirsch-Ernst K, Wölfl S, Hellwig F. The chloroplast genome of the 'basal' angiosperm Calycanthus fertilis–structural and phylogenetic analyses. Plant Syst Evol. 2003;242:119–35.

    CAS  Article  Google Scholar 

  24. 24.

    Goremykin VV, Hirsch-Ernst KI, Wolfl S, Hellwig FH. The chloroplast genome of Nymphaea alba: whole-genome analyses and the problem of identifying the most basal angiosperm. Mol Biol Evol. 2004;21:1445–54.

    CAS  PubMed  Article  Google Scholar 

  25. 25.

    Goremykin VV, Hirsch-Ernst KI, Wölfl S, Hellwig FH. Analysis of the Amborella trichopoda chloroplast genome sequence suggests that Amborella is not a basal angiosperm. Mol Biol Evol. 2003;20:1499–505.

    CAS  PubMed  Article  Google Scholar 

  26. 26.

    Dhingra A, Folta KM. ASAP: amplification, sequencing & annotation of plastomes. BMC Genomics. 2005;6:176.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  27. 27.

    Heinze B. A database of PCR primers for the chloroplast genomes of higher plants. Plant Methods. 2007;3:4.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  28. 28.

    Wu FH, Kan DP, Lee SB, Daniell H, Lee YW, Lin CC, et al. Complete nucleotide sequence of Dendrocalamus latiflorus and Bambusa oldhamii chloroplast genomes. Tree Physiol. 2009;29:847–56.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  29. 29.

    Leseberg CH, Duvall MR. The complete chloroplast genome of Coix lacryma-jobi and a comparative molecular evolutionary analysis of plastomes in cereals. J Mol Evol. 2009;69:311–8.

    CAS  PubMed  Article  Google Scholar 

  30. 30.

    Dong W, Xu C, Cheng T, Lin K, Zhou S. Sequencing angiosperm plastid genomes made easy: a complete set of universal primers and a case study on the phylogeny of saxifragales. Genome Biol Evol. 2013;5:989–97.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  31. 31.

    Mardanov AV, Ravin NV, Kuznetsov BB, Samigullin TH, Antonov AS, Kolganova TV, Skyabin KG. Complete sequence of the duckweed (Lemna minor) chloroplast genome: structural organization and phylogenetic relationships to other angiosperms. J Mol Evol. 2008;66:555–64.

    CAS  PubMed  Article  Google Scholar 

  32. 32.

    Lin CS, Chen JJ, Huang YT, Chan MT, Daniell H, Chang WJ, et al. The location and translocation of ndh genes of chloroplast origin in the Orchidaceae family. Sci Rep. 2015;5:9040.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  33. 33.

    Moore MJ, Dhingra A, Soltis PS, Shaw R, Farmerie WG, Folta KM, Soltis DE. Rapid and accurate pyrosequencing of angiosperm plastid genomes. BMC Plant Biol. 2006;6:17.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  34. 34.

    Wang W, Messing J. High-throughput sequencing of three Lemnoideae (duckweeds) chloroplast genomes from total DNA. PLoS One. 2011;6, e24670.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  35. 35.

    Cronn R, Liston A, Parks M, Gernandt DS, Shen R, Mockler T. Multiplex sequencing of plant chloroplast genomes using Solexa sequencing-by-synthesis technology. Nucleic Acids Res. 2008;36, e122.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  36. 36.

    Pan IC, Liao DC, Wu FH, Daniell H, Singh ND, Chang C, et al. Complete chloroplast genome sequence of an orchid model plant candidate: Erycina pusilla apply in tropical oncidium breeding. PLoS One. 2012;7, e34738.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  37. 37.

    Atherton RA, McComish BJ, Shepherd LD, Berry LA, Albert NW, Lockhart PJ. Whole genome sequencing of enriched chloroplast DNA using the Illumina GAII platform. Plant Methods. 2010;6:22.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  38. 38.

    Jackman SD, Warren RL, Gibb EA, Vandervalk BP, Mohamadi H, Chu J, et al. Organellar genomes of white spruce (Picea glauca): assembly and annotation. Genome Biol Evol. 2016;8:29–41.

    Article  Google Scholar 

  39. 39.

    Ferrarini M, Moretto M, Ward JA, Šurbanovski N, Stevanović V, Giongo L, et al. An evaluation of the PacBio RS platform for sequencing and de novo assembly of a chloroplast genome. BMC Genomics. 2013;14:670.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  40. 40.

    Wu Z, Gui S, Quan Z, Pan L, Wang S, Ke W, et al. A precise chloroplast genome of Nelumbo nucifera (Nelumbonaceae) evaluated with Sanger, Illumina MiSeq, and PacBio RS II sequencing platforms: insight into the plastid evolution of basal eudicots. BMC Plant Biol. 2014;14:289.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  41. 41.

    Li Q, Li Y, Song J, Xu H, Xu J, Zhu Y, et al. High‐accuracy de novo assembly and SNP detection of chloroplast genomes using a SMRT circular consensus sequencing strategy. New Phytol. 2014;204:1041–9.

    CAS  PubMed  Article  Google Scholar 

  42. 42.

    Redwan R, Saidin A, Kumar S. Complete chloroplast genome sequence of MD-2 pineapple and its comparative analysis among nine other plants from the subclass Commelinidae. BMC Plant Biol. 2015;15:196.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  43. 43.

    Chen X, Li Q, Li Y, Qian J, Han J. Chloroplast genome of Aconitum barbatum var. puberulum (Ranunculaceae) derived from CCS reads using the PacBio RS platform. Frontiers Plant Sci. 2015;6:42.

    Google Scholar 

  44. 44.

    Eid J, Fehr A, Gray J, Luong K, Lyle J, Otto G, et al. Real-time DNA sequencing from single polymerase molecules. Science. 2009;323:133–8.

    CAS  PubMed  Article  Google Scholar 

  45. 45.

    English AC, Richards S, Han Y, Wang M, Vee V, Qu J, et al. Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology. PLoS One. 2012;7, e47768.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  46. 46.

    Chin CS, Alexander DH, Marks P, Klammer AA, Drake J, Heiner C, et al. Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data. Nat Methods. 2013;10:563–9.

    CAS  PubMed  Article  Google Scholar 

  47. 47.

    Oldenburg DJ, Bendich AJ. DNA maintenance in plastids and mitochondria of plants. Frontiers Plant Sci. 2015;6:883.

    Article  Google Scholar 

  48. 48.

    Oldenburg DJ, Bendich AJ. The linear plastid chromosomes of maize: terminal sequences, structures, and implications for DNA replication. Curr Genet. 2016;62:431–42.

    CAS  PubMed  Article  Google Scholar 

  49. 49.

    Jansen RK, Cai Z, Raubeson LA, Daniell H, Leebens-Mack J, Müller KF, et al. Analysis of 81 genes from 64 plastid genomes resolves relationships in angiosperms and identifies genome-scale evolutionary patterns. Proc Natl Acad Sci U S A. 2007;104:19369–74.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  50. 50.

    Pillon Y, Chase MW. Taxonomic exaggeration and its effects on orchid conservation. Conserv Biol. 2007;21:263–5.

    PubMed  Article  Google Scholar 

  51. 51.

    Chang CC, Lin HC, Lin IP, Chow TY, Chen HH, Chen WH, et al. The chloroplast genome of Phalaenopsis aphrodite (Orchidaceae): comparative analysis of evolutionary rate with that of grasses and its phylogenetic implications. Mol Biol Evol. 2006;23:279–91.

    CAS  PubMed  Article  Google Scholar 

  52. 52.

    Jheng CF, Chen TC, Lin JY, Chen TC, Wu WL, Chang CC. The comparative chloroplast genomic analysis of photosynthetic orchids and developing DNA markers to distinguish Phalaenopsis orchids. Plant Sci. 2012;190:62–73.

    CAS  PubMed  Article  Google Scholar 

  53. 53.

    Kim GB, Kwon Y, Yu HJ, Lim KB, Seo JH, Mun JH. The complete chloroplast genome of Phalaenopsis 'Tiny Star'. Mitochondrial DNA. 2016;27:1300–2.

    CAS  PubMed  Article  Google Scholar 

  54. 54.

    Wu FH, Chan MT, Liao DC, Hsu CT, Lee YW, Daniell H, et al. Complete chloroplast genome of Oncidium Gower Ramsey and evaluation of molecular markers for identification and breeding in Oncidiinae. BMC Plant Biol. 2010;10:68.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  55. 55.

    Yang JB, Tang M, Li HT, Zhang ZR, Li DZ. Complete chloroplast genome of the genus Cymbidium: lights into the species identification, phylogenetic implications and population genetic analyses. BMC Evol Biol. 2013;13:84.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  56. 56.

    Luo J, Hou BW, Niu ZT, Liu W, Xue QY, Ding XY. Comparative chloroplast genomes of photosynthetic orchids: insights into evolution of the Orchidaceae and development of molecular markers for phylogenetic applications. PLoS One. 2014;9, e99016.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  57. 57.

    Li J, Chen C, Wang ZZ. The complete chloroplast genome of the Dendrobium strongylanthum (Orchidaceae: Epidendroideae). Mitochondrial DNA. 2016;27:3048–9.

    PubMed  Google Scholar 

  58. 58.

    da Rocha Perini V, Leles B, Furtado C, Prosdocimi F. Complete chloroplast genome of the orchid Cattleya crispata (Orchidaceae:Laeliinae), a Neotropical rupiculous species. Mitochondrial DNA. 2015. doi:10.3109/19401736.2014.1003850.

  59. 59.

    Tsitrone A, Kirkpatrick M, Levin DA. A model for chloroplast capture. Evolution. 2003;57:1776–82.

    PubMed  Article  Google Scholar 

  60. 60.

    Soltis DE, Kuzoff RK. Discordance between nuclear and chloroplast phylogenies in the heuchera group (Saxifragaceae). Evolution. 1995;49:727–42.

    Article  Google Scholar 

  61. 61.

    Rieseberg LH, Soltis DE. Phylogenetic consequences of cytoplasmic gene flow in plants. Evol Trends Plants. 1991;5:65–84.

    Google Scholar 

  62. 62.

    Maier RM, Neckermann K, Igloi GL, Kossel H. Complete sequence of the maize chloroplast genome: gene content, hotspots of divergence and fine tuning of genetic information by transcript editing. J Mol Biol. 1995;251:614–28.

    CAS  PubMed  Article  Google Scholar 

  63. 63.

    Ogihara Y, Isono K, Kojima T, Endo A, Hanaoka M, Shiina T, et al. Structural features of a wheat plastome as revealed by complete sequencing of chloroplast DNA. Mol Genet Genomics. 2002;266:740–6.

    CAS  PubMed  Article  Google Scholar 

  64. 64.

    Middleton CP, Senerchia N, Stein N, Akhunov ED, Keller B, Wicker T, Kilian B. Sequencing of chloroplast genomes from wheat, barley, rye and their relatives provides a detailed insight into the evolution of the Triticeae tribe. PLoS One. 2014;9, e85761.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  65. 65.

    Brozynska M, Omar ES, Furtado A, Crayn D, Simon B, Ishikawa R, Henry RJ. Chloroplast genome of novel rice germplasm identified in northern Australia. Tropical Plant Biol. 2014;7:111–20.

    CAS  Article  Google Scholar 

  66. 66.

    Waters DL, Nock CJ, Ishikawa R, Rice N, Henry RJ. Chloroplast genome sequence confirms distinctness of Australian and Asian wild rice. Ecol Evol. 2012;2:211–7.

    PubMed  PubMed Central  Article  Google Scholar 

  67. 67.

    Sotowa M, Ootsuka K, Kobayashi Y, Hao Y, Tanaka K, Ichitani K, et al. Molecular relationships between Australian annual wild rice, Oryza meridionalis, and two related perennial forms. Rice. 2013;6:26.

    PubMed  PubMed Central  Article  Google Scholar 

  68. 68.

    Xu Q, Xiong G, Li P, He F, Huang Y, Wang K, et al. Analysis of complete nucleotide sequences of 12 Gossypium chloroplast genomes: origin and evolution of allotetraploids. PLoS One. 2012;7, e37128.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  69. 69.

    Ibrahim RI, Azuma J, Sakamoto M. Complete nucleotide sequence of the cotton (Gossypium barbadense L.) chloroplast genome with a comparative analysis of sequences among 9 dicot plants. Genes Genet Syst. 2006;81:311–21.

    CAS  PubMed  Article  Google Scholar 

  70. 70.

    Li P, Li Z, Liu H, Hua J. Cytoplasmic diversity of the cotton genus as revealed by chloroplast microsatellite markers. Genet Resour Crop Evol. 2014;61:107–19.

    CAS  Article  Google Scholar 

  71. 71.

    Pickersgill B, Debouck DG. Domestication patterns in common bean (Phaseolus vulgaris L.) and the origin of the Mesoamerican and Andean cultivated races. Theor Appl Genet. 2005;110:432–44.

    PubMed  Article  CAS  Google Scholar 

  72. 72.

    Palmer JD, Osorio B, Thompson WF. Evolutionary significance of inversions in legume chloroplast DNAs. Curr Genet. 1988;14:65–74.

    CAS  Article  Google Scholar 

  73. 73.

    Sherman-Broyles S, Bombarely A, Grimwood J, Schmutz J, Doyle J. Complete plastome sequences from Glycine syndetika and six additional perennial wild relatives of soybean. G3 (Bethesda). 2014;4:2023–33.

    CAS  Article  Google Scholar 

  74. 74.

    Sabir J, Schwarz E, Ellison N, Zhang J, Baeshen NA, Mutwakil M, et al. Evolutionary and biotechnology implications of plastid genome variation in the inverted-repeat-lacking clade of legumes. Plant Biotechnol J. 2014;12:743–54.

    CAS  PubMed  Article  Google Scholar 

  75. 75.

    Cai Z, Guisinger M, Kim H-G, Ruck E, Blazier JC, McMurtry V, et al. Extensive reorganization of the plastid genome of Trifolium subterraneum (Fabaceae) is associated with numerous repeated sequences and novel DNA insertions. J Mol Evol. 2008;67:696–704.

    CAS  PubMed  Article  Google Scholar 

  76. 76.

    Magee AM, Aspinall S, Rice DW, Cusack BP, Semon M, Perry AS, et al. Localized hypermutation and associated gene losses in legume chloroplast genomes. Genome Res. 2010;20:1700–10.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  77. 77.

    Bogdanova VS, Zaytseva OO, Mglinets AV, Shatskaya NV, Kosterin OE, Vasiliev GV. Nuclear-cytoplasmic conflict in pea (Pisum sativum L.) is associated with nuclear and plastidic candidate genes encoding acetyl-CoA carboxylase subunits. PLoS One. 2015;10:e0119835.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  78. 78.

    Guo X, Castillo-Ramírez S, González V, Bustos P, Fernández-Vázquez JL, Santamaría RI, et al. Rapid evolutionary change of common bean (Phaseolus vulgaris L.) plastome, and the genomic diversification of legume chloroplasts. BMC Genomics. 2007;8:228.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  79. 79.

    Tangphatsornruang S, Sangsrakru D, Chanprasert J, Uthaipaisanwong P, Yoocha T, Jomchai N, Tragoonrung S. The chloroplast genome sequence of mungbean (Vigna radiata) determined by high-throughput pyrosequencing: structural organization and phylogenetic relationships. DNA Res. 2010;17:11–22.

    CAS  PubMed  Article  Google Scholar 

  80. 80.

    Martin GE, Rousseau-Gueutin M, Cordonnier S, Lima O, Michon-Coudouel S, Naquin D, et al. The first complete chloroplast genome of the Genistoid legume Lupinus luteus: evidence for a novel major lineage-specific rearrangement and new insights regarding plastome evolution in the legume family. Ann Bot. 2014;113:1197–210.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  81. 81.

    Kazakoff SH, Imelfort M, Edwards D, Koehorst J, Biswas B, Batley J, et al. Capturing the biofuel wellhead and powerhouse: the chloroplast and mitochondrial genomes of the leguminous feedstock tree Pongamia pinnata. PLoS One. 2012;7, e51687.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  82. 82.

    Schwarz EN, Ruhlman TA, Sabir JS, Hajrah NH, Alharbi NS, Al‐Malki AL, et al. Plastid genome sequences of legumes reveal parallel inversions and multiple losses of rps16 in papilionoids. J Syst Evol. 2015;53:458–68.

    Article  Google Scholar 

  83. 83.

    Su HJ, Hogenhout SA, Al-Sadi AM, Kuo CH. Complete chloroplast genome sequence of Omani Lime (Citrus aurantiifolia) and comparative analysis within the Rosids. PLoS One. 2014;9, e113049.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  84. 84.

    Carbonell-Caballero J, Alonso R, Ibañez V, Terol J, Talon M, Dopazo J. A phylogenetic analysis of 34 chloroplast genomes elucidates the relationships between wild and domestic species within the genus Citrus. Mol Biol Evol. 2015;32:2015–35.

    PubMed  PubMed Central  Article  Google Scholar 

  85. 85.

    Caspermeyer J. Most comprehensive study to date reveals evolutionary history of Citrus. Mol Biol Evol. 2015;32:2217–8.

    Google Scholar 

  86. 86.

    Chen SL, Xiao PG. Molecular evolution and positive Darwinian selection of the chloroplast maturase matK. J Plant Res. 2010;123:241–7.

    PubMed  Article  CAS  Google Scholar 

  87. 87.

    Peng L, Yamamoto H, Shikanai T. Structure and biogenesis of the chloroplast NAD(P)H dehydrogenase complex. Biochim Biophys Acta. 1807;2011:945–53.

    Google Scholar 

  88. 88.

    Ma PF, Zhang YX, Guo ZH, Li DZ. Evidence for horizontal transfer of mitochondrial DNA to the plastid genome in a bamboo genus. Sci Rep. 2015;5:11608.

    PubMed  PubMed Central  Article  Google Scholar 

  89. 89.

    Zhang YJ, Ma PF, Li DZ. High-throughput sequencing of six bamboo chloroplast genomes: phylogenetic implications for temperate woody bamboos (Poaceae: Bambusoideae). PLoS One. 2011;6, e20596.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  90. 90.

    Burke SV, Grennan CP, Duvall MR. Plastome sequences of two New World bamboos–Arundinaria gigantea and Cryptochloa strictiflora (Poaceae)–extend phylogenomic understanding of Bambusoideae. Am J Bot. 2012;99:1951–61.

    CAS  PubMed  Article  Google Scholar 

  91. 91.

    Gao J, Li K, Gao LZ. The complete chloroplast genome sequence of the Bambusa multiplex (Poaceae: Bambusoideae). Mitochondrial DNA. 2014;18:1–3.

    Google Scholar 

  92. 92.

    Wysocki WP, Clark LG, Attigala L, Ruiz-Sanchez E, Duvall MR. Evolution of the bamboos (Bambusoideae; Poaceae): a full plastome phylogenomic analysis. BMC Evol Biol. 2015;15:50.

    PubMed  PubMed Central  Article  Google Scholar 

  93. 93.

    Ma PF, Zhang YX, Zeng CX, Guo ZH, Li DZ. Chloroplast phylogenomic analyses resolve deep-level relationships of an intractable bamboo tribe Arundinarieae (Poaceae). Syst Biol. 2014;63:933–50.

    PubMed  Article  Google Scholar 

  94. 94.

    Smith DR. Mitochondrion-to-plastid DNA, transfer: it happens. New Phytol. 2014;202:736–8.

    CAS  PubMed  Article  Google Scholar 

  95. 95.

    Timmis JN, Ayliffe MA, Huang CY, Martin W. Endosymbiotic gene transfer: organelle genomes forge eukaryotic chromosomes. Nat Rev Genet. 2004;5:123–35.

    CAS  PubMed  Article  Google Scholar 

  96. 96.

    Li H, Chiu CC. Protein transport into chloroplasts. Annu Rev Plant Biol. 2010;61:157–80.

    CAS  PubMed  Article  Google Scholar 

  97. 97.

    Barrett CF, Freudenstein JV, Li J, Mayfield-Jones DR, Perez L, Pires JC, Santos C. Investigating the path of plastid genome degradation in an early-transitional clade of heterotrophic orchids, and implications for heterotrophic angiosperms. Mol Biol Evol. 2014;31:3095–112.

    CAS  PubMed  Article  Google Scholar 

  98. 98.

    Tiller N, Bock R. The translational apparatus of plastids and its role in plant development. Mol Plant. 2014;7:1105–20.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  99. 99.

    Hazkani-Covo E, Zeller RM, Martin W. Molecular poltergeists: mitochondrial DNA copies (numts) in sequenced nuclear genomes. PLoS Genet. 2010;6, e1000834.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  100. 100.

    Smith DR, Crosby K, Lee RW. Correlation between nuclear plastid DNA abundance and plastid number supports the limited transfer window hypothesis. Genome Biol Evol. 2011;3:365–71.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  101. 101.

    Cummings HS, Hershey JWB. Translation initiation-factor IF1 is essential for cell viability in Escherichia coli. J Bacteriol. 1994;176:198–205.

    CAS  PubMed  PubMed Central  Google Scholar 

  102. 102.

    Millen RS, Olmstead RG, Adams KL, Palmer JD, Lao NT, Heggie L, et al. Many parallel losses of infA from chloroplast DNA during angiosperm evolution with multiple independent transfers to the nucleus. Plant Cell. 2001;13:645–58.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  103. 103.

    Gantt JS, Baldauf SL, Calie PJ, Weeden NF, Palmer JD. Transfer of rpl22 to the nucleus greatly preceded its loss from the chloroplast and involved the gain of an intron. EMBO J. 1991;10:3073–8.

    CAS  PubMed  PubMed Central  Google Scholar 

  104. 104.

    Park S, Jansen RK, Park S. Complete plastome sequence of Thalictrum coreanum (Ranunculaceae) and transfer of the rpl32 gene to the nucleus in the ancestor of the subfamily Thalictroideae. BMC Plant Biol. 2015;15:40.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  105. 105.

    Ueda M, Fujimoto M, Arimura S, Murata J, Tsutsumi N, Kadowaki K. Loss of the rpl32 gene from the chloroplast genome and subsequent acquisition of a preexisting transit peptide within the nuclear gene in Populus. Gene. 2007;402:51–6.

    CAS  PubMed  Article  Google Scholar 

  106. 106.

    Cusack BP, Wolfe KH. When gene marriages don’t work out: divorce by subfunctionalization. Trends Genet. 2007;23:270–2.

    CAS  PubMed  Article  Google Scholar 

  107. 107.

    Ueda M, Kuniyoshi T, Yamamoto H, Sugimoto K, Ishizaki K, Kohchi T, et al. Composition and physiological function of the chloroplast NADH dehydrogenase-like complex in Marchantia polymorpha. Plant J. 2012;72:683–93.

    CAS  PubMed  Article  Google Scholar 

  108. 108.

    Munekage Y, Hashimoto M, Miyake C, Tomizawa K-I, Endo T, Tasaka M, Shikanai T. Cyclic electron flow around photosystem I is essential for photosynthesis. Nature. 2004;429:579–82.

    CAS  PubMed  Article  Google Scholar 

  109. 109.

    Peltier G, Cournac L. Chlororespiration. Annu Rev Plant Biol. 2002;53:523–50.

    CAS  PubMed  Article  Google Scholar 

  110. 110.

    Wakasugi T, Tsudzuki J, Ito S, Nakashima K, Tsudzuki T, Sugiura M. Loss of all ndh genes as determined by sequencing the entire chloroplast genome of the black pine Pinus thunbergii. Proc Natl Acad Sci U S A. 1994;91:9794–8.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  111. 111.

    Sanderson MJ, Copetti D, Búrquez A, Bustamante E, Charboneau JL, Eguiarte LE, et al. Exceptional reduction of the plastid genome of saguaro cactus (Carnegiea gigantea): loss of the ndh gene suite and inverted repeat. Am J Bot. 2015;102:1115–27.

    PubMed  Article  Google Scholar 

  112. 112.

    McCoy SR, Kuehl JV, Boore JL, Raubeson LA. The complete plastid genome sequence of Welwitschia mirabilis: an unusually compact plastome with accelerated divergence rates. BMC Evol Biol. 2008;8:130.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  113. 113.

    Braukmann TWA, Kuzmina M, Stefanović S. Loss of all plastid ndh genes in Gnetales and conifers: extent and evolutionary significance for the seed plant phylogeny. Curr Genet. 2009;55:323–37.

    CAS  PubMed  Article  Google Scholar 

  114. 114.

    Blazier J, Guisinger MM, Jansen RK. Recent loss of plastid-encoded ndh genes within Erodium (Geraniaceae). Plant Mol Biol. 2011;76:263–72.

    CAS  Article  Google Scholar 

  115. 115.

    Weng M-L, Blazier JC, Govindu M, Jansen RK. Reconstruction of the ancestral plastid genome in Geraniaceae reveals a correlation between genome rearrangements, repeats and nucleotide substitution rates. Mol Biol Evol. 2014;31:645–59.

    CAS  PubMed  Article  Google Scholar 

  116. 116.

    Ranade SS, García-Gil MR, Rosselló JA. Non-functional plastid ndh gene fragments are present in the nuclear genome of Norway spruce (Picea abies L. Karsch): insights from in silico analysis of nuclear and organellar genomes. Mol Genet Genomics. 2016;291:935–41.

    CAS  PubMed  Article  Google Scholar 

  117. 117.

    Ruhlman TA, Chang WJ, Chen JJ, Huang YT, Chan MT, Zhang J, et al. NDH expression marks major transitions in plant evolution and reveals coordinate intracellular gene loss. BMC Plant Biol. 2015;15:100.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  118. 118.

    Takabayashi A, Endo T, Shikanai T, Sato F. Post-illumination reduction of the plastoquinone pool in chloroplast transformants in which chloroplastic NAD(P)H dehydrogenase was inactivated. Biosci Biotechnol Biochem. 2002;66:2107–11.

    CAS  PubMed  Article  Google Scholar 

  119. 119.

    Burrows PA, Sazanov LA, Svab Z, Maliga P, Nixon PJ. Identification of a functional respiratory complex in chloroplasts through analysis of tobacco mutants containing disrupted plastid ndh genes. EMBO J. 1998;17:868–76.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  120. 120.

    Kofer W, Koop H-U, Wanner G, Steinmüller K. Mutagenesis of the genes encoding subunits A, C, H, I, J and K of the plastid NAD(P)H-plastoquinone-oxidoreductase in tobacco by polyethylene glycol-mediated plastome transformation. Mol Gen Genet. 1998;258:166–73.

    CAS  PubMed  Article  Google Scholar 

  121. 121.

    Shikanai T, Endo T, Hashimoto T, Yamada Y, Asada K, Yokota A. Directed disruption of the tobacco ndhB gene impairs cyclic electron flow around photosystem I. Proc Natl Acad Sci U S A. 1998;95:9705–9.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  122. 122.

    Jin S, Daniell H. The engineered chloroplast genome just got smarter. Trends Plant Sci. 2015;20:622–40.

    CAS  PubMed  Article  Google Scholar 

  123. 123.

    Daniell H. Transgene containment by maternal inheritance: effective or elusive? Proc Natl Acad Sci U S A. 2007;104:6879–80.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  124. 124.

    Dheeraj V, Daniell H. Chloroplast vector systems for biotechnology applications. Plant Physiol. 2007;145:1129–43.

    Article  CAS  Google Scholar 

  125. 125.

    Dunne A, Maple‐Grødem J, Gargano D, Haslam RP, Napier JA, Chua NH, et al. Modifying fatty acid profiles through a new cytokinin‐based plastid transformation system. Plant J. 2014;80:1131–8.

    CAS  PubMed  Article  Google Scholar 

  126. 126.

    Kavanagh TA, Thanh ND, Lao NT, McGrath N, Peter SO, Horváth EM, et al. Homeologous plastid DNA transformation in tobacco is mediated by multiple recombination events. Genetics. 1999;152:1111–22.

    CAS  PubMed  PubMed Central  Google Scholar 

  127. 127.

    Guda C, Lee SB, Daniell H. Stable expression of a biodegradable protein-based polymer in tobacco chloroplasts. Plant Cell Rep. 2000;19:257–62.

    CAS  Article  Google Scholar 

  128. 128.

    Ruhlman T, Verma D, Samson N, Daniell H. The role of heterologous chloroplast sequence elements in transgene integration and expression. Plant Physiol. 2010;152:2088–104.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  129. 129.

    Daniell H, Datta R, Varma S, Gray S, Lee SB. Containment of herbicide resistance through genetic engineering of the chloroplast genome. Nat Biotechnol. 1998;16:345–8.

    CAS  PubMed  Article  Google Scholar 

  130. 130.

    Krichevsky A, Meyers B, Vainstein A, Maliga P, Citovsky V. Autoluminescent plants. PLoS One. 2010;5, e15461.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  131. 131.

    Daniell H, Vivekananda J, Nielsen B, Ye G, Tewari K, Sanford J. Transient foreign gene expression in chloroplasts of cultured tobacco cells after biolistic delivery of chloroplast vectors. Proc Natl Acad Sci U S A. 1990;87:88–92.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  132. 132.

    Klein RR, Mullet JE. Control of gene expression during higher plant chloroplast biogenesis. Protein synthesis and transcript levels of psbA, psaA-psaB, and rbcL in dark-grown and illuminated barley seedlings. J Biol Chem. 1987;262:4341–8.

    CAS  PubMed  Google Scholar 

  133. 133.

    Wurbs D, Ruf S, Bock R. Contained metabolic engineering in tomatoes by expression of carotenoid biosynthesis genes from the plastid genome. Plant J. 2007;49:276–88.

    CAS  PubMed  Article  Google Scholar 

  134. 134.

    McBride KE, Schaaf DJ, Daley M, Stalker DM. Controlled expression of plastid transgenes in plants based on a nuclear DNA-encoded and plastid-targeted T7 RNA polymerase. Proc Natl Acad Sci U S A. 1994;91:7301–5.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  135. 135.

    Kumar S, Dhingra A, Daniell H. Plastid-expressed betaine aldehyde dehydrogenase gene in carrot cultured cells, roots, and leaves confers enhanced salt tolerance. Plant Physiol. 2004;136:2843–54.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  136. 136.

    Dhingra A, Portis Jr AR, Daniell H. Enhanced translation of a chloroplast-expressed RbcS gene restores small subunit levels and photosynthesis in nuclear RbcS antisense plants. Proc Natl Acad Sci U S A. 2004;101:6315–20.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  137. 137.

    De Cosa B, Moar W, Lee SB, Miller M, Daniell H. Overexpression of the Bt cry2Aa2 operon in chloroplasts leads to formation of insecticidal crystals. Nat Biotechnol. 2001;19:71–4.

    PubMed  PubMed Central  Article  Google Scholar 

  138. 138.

    Ruf S, Hermann M, Berger IJ, Carrer H, Bock R. Stable genetic transformation of tomato plastids and expression of a foreign protein in fruit. Nat Biotechnol. 2001;19:870–5.

    CAS  PubMed  Article  Google Scholar 

  139. 139.

    Zhou F, Badillo-Corona JA, Karcher D, Gonzalez-Rabade N, Piepenburg K, Borchers AM, et al. High-level expression of human immunodeficiency virus antigens from the tobacco and tomato plastid genomes. Plant Biotechnol J. 2008;6:897–913.

    CAS  PubMed  Article  Google Scholar 

  140. 140.

    Lee SB, Li B, Jin S, Daniell H. Expression and characterization of antimicrobial peptides Retrocyclin-101 and Protegrin-1 in chloroplasts to control viral and bacterial infections. Plant Biotechnol J. 2011;9:100–15.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  141. 141.

    Jin S, Kanagaraj A, Verma D, Lange T, Daniell H. Release of hormones from conjugates: chloroplast expression of beta-glucosidase results in elevated phytohormone levels associated with significant increase in biomass and protection from aphids or whiteflies conferred by sucrose esters. Plant Physiol. 2011;155:222–35.

    CAS  PubMed  Article  Google Scholar 

  142. 142.

    Jin S, Zhang X, Daniell H. Pinellia ternata agglutinin expression in chloroplasts confers broad spectrum resistance against aphid, whitefly, Lepidopteran insects, bacterial and viral pathogens. Plant Biotechnol J. 2012;10:313–27.

    CAS  PubMed  Article  Google Scholar 

  143. 143.

    Liu CW, Lin CC, Chen JJ, Tseng MJ. Stable chloroplast transformation in cabbage (Brassica oleracea L. var. capitata L.) by particle bombardment. Plant Cell Rep. 2007;26:1733–44.

    CAS  PubMed  Article  Google Scholar 

  144. 144.

    Dufourmantel N, Pelissier B, Garcon F, Peltier G, Ferullo JM, Tissot G. Generation of fertile transplastomic soybean. Plant Mol Biol. 2004;55:479–89.

    CAS  PubMed  Article  Google Scholar 

  145. 145.

    Dufourmantel N, Tissot G, Goutorbe F, Garcon F, Muhr C, Jansens S, et al. Generation and analysis of soybean plastid transformants expressing Bacillus thuringiensis Cry1Ab protoxin. Plant Mol Biol. 2005;58:659–68.

    CAS  PubMed  Article  Google Scholar 

  146. 146.

    Singh AK, Verma SS, Bansal KC. Plastid transformation in eggplant (Solanum melongena L.). Transgenic Res. 2010;19:113–9.

    CAS  PubMed  Article  Google Scholar 

  147. 147.

    Jin S, Singh ND, Li L, Zhang X, Daniell H. Engineered chloroplast dsRNA silences cytochrome p450 monooxygenase, V-ATPase and chitin synthase genes in the insect gut and disrupts Helicoverpa armigera larval development and pupation. Plant Biotechnol J. 2015;13:435–46.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  148. 148.

    Zhang J, Khan SA, Hasse C, Ruf S, Heckel DG, Bock R. Pest control. Full crop protection from an insect pest by expression of long double-stranded RNAs in plastids. Science. 2015;347:991–4.

    CAS  PubMed  Article  Google Scholar 

  149. 149.

    Viitanen PV, Devine AL, Khan MS, Deuel DL, Van Dyk DE, Daniell H. Metabolic engineering of the chloroplast genome using the Echerichia coli ubiC gene reveals that chorismate is a readily abundant plant precursor for p-hydroxybenzoic acid biosynthesis. Plant Physiol. 2004;136:4048–60.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  150. 150.

    Verma D, Kanagaraj A, Jin SX, Singh ND, Kolattukudy PE, Daniell H. Chloroplast-derived enzyme cocktails hydrolyse lignocellulosic biomass and release fermentable sugars. Plant Biotechnol J. 2010;8:332–50.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  151. 151.

    Schneider C. Chemistry and biology of vitamin E. Mol Nutr Food Res. 2005;49:7–30.

    CAS  PubMed  Article  Google Scholar 

  152. 152.

    Shintani D, DellaPenna D. Elevating the vitamin E content of plants through metabolic engineering. Science. 1998;282:2098–100.

    CAS  PubMed  Article  Google Scholar 

  153. 153.

    Jin S, Daniell H. Expression of gamma-tocopherol methyltransferase in chloroplasts results in massive proliferation of the inner envelope membrane and decreases susceptibility to salt and metal-induced oxidative stresses by reducing reactive oxygen species. Plant Biotechnol J. 2014;12:1274–85.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  154. 154.

    Apel W, Bock R. Enhancement of carotenoid biosynthesis in transplastomic tomatoes by induced lycopene-to-provitamin A conversion. Plant Physiol. 2009;151:59–66.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  155. 155.

    Grabowski H, Cockburn I, Long G. The market for follow-on biologics: how will it evolve? Health Aff. 2006;25:1291–301.

    Article  Google Scholar 

  156. 156.

    Spök A, Karner S, Stein AJ, Rodríguez-Cerezo E. Plant molecular farming. Opportunities and challenges. JRC Scientific and Technical Reports 2008. http://ftp.jrc.es/EURdoc/JRC43873.pdf. Accessed 17 May 2016.

  157. 157.

    Holtz BR, Berquist BR, Bennett LD, Kommineni VJ, Munigunti RK, White EL, et al. Commercial‐scale biotherapeutics manufacturing facility for plant‐made pharmaceuticals. Plant Biotechnol J. 2015;13:1180–90.

    CAS  PubMed  Article  Google Scholar 

  158. 158.

    Kwon KC, Daniell H. Low‐cost oral delivery of protein drugs bioencapsulated in plant cells. Plant Biotechnol J. 2015;13:1017–22.

    PubMed  PubMed Central  Article  Google Scholar 

  159. 159.

    El Kaoutari A, Armougom F, Gordon JI, Raoult D, Henrissat B. The abundance and variety of carbohydrate-active enzymes in the human gut microbiota. Nat Rev Microbiol. 2013;11:497–504.

    PubMed  Article  CAS  Google Scholar 

  160. 160.

    Kwon KC, Nityanandam R, New JS, Daniell H. Oral delivery of bioencapsulated exendin-4 expressed in chloroplasts lowers blood glucose level in mice and stimulates insulin secretion in beta-TC6 cells. Plant Biotechnol J. 2013;11:77–86.

    CAS  PubMed  Article  Google Scholar 

  161. 161.

    Shenoy V, Kwon KC, Rathinasabapathy A, Lin SN, Jin GY, Song CJ, et al. Oral delivery of angiotensin-converting enzyme 2 and angiotensin-(1–7) bioencapsulated in plant cells attenuates pulmonary hypertension. Hypertension. 2014;64:1248–59.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  162. 162.

    Kohli N, Westerveld DR, Ayache AC, Verma A, Shil P, Prasad T, et al. Oral delivery of bioencapsulated proteins across blood–brain and blood-retinal barriers. Mol Ther. 2014;22:535–46.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  163. 163.

    Chan HT, Daniell H. Plant‐made oral vaccines against human infectious diseases—are we there yet? Plant Biotechnol J. 2015;13:1056–70.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  164. 164.

    Davoodi-Semiromi A, Schreiber M, Nalapalli S, Verma D, Singh ND, Banks RK, et al. Chloroplast-derived vaccine antigens confer dual immunity against cholera and malaria by oral or injectable delivery. Plant Biotechnol J. 2010;8:223–42.

    CAS  PubMed  Article  Google Scholar 

  165. 165.

    Lakshmi PS, Verma D, Yang X, Lloyd B, Daniell H. Low cost tuberculosis vaccine antigens in capsules: expression in chloroplasts, bio-encapsulation, stability and functional evaluation in vitro. PLoS One. 2013;8, e54708.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  166. 166.

    Hagelberg E, Hofreiter M, Keyser C. Ancient DNA: the first three decades. Philos Trans R Soc Lond B Biol Sci. 2015;370:20130371.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  167. 167.

    Golenberg EM, Giannasi DE, Clegg MT, Smiley CJ, Durbin M, Henderson D, et al. Chloroplast DNA sequence from a Miocene Magnolia species. Nature. 1990;344:656–8.

    CAS  PubMed  Article  Google Scholar 

  168. 168.

    Rosselló JA. The never‐ending story of geologically ancient DNA: was the model plant Arabidopsis the source of Miocene Dominican amber? Biol J Linn Soc. 2014;111:234–40.

    Article  Google Scholar 

  169. 169.

    Zhu A, Guo W, Gupta S, Fan W, Mower JP. Evolutionary dynamics of the plastid inverted repeat: the effects of expansion, contraction, and loss on substitution rates. New Phytol. 2016;209:1747–56.

    CAS  PubMed  Article  Google Scholar 

  170. 170.

    von Kohn C, Kielkowska A, Havey MJ. Sequencing and annotation of the chloroplast DNAs and identification of polymorphisms distinguishing normal male-fertile and male-sterile cytoplasms of onion. Genome. 2013;56:737–42.

    Article  CAS  Google Scholar 

  171. 171.

    Nashima K, Terakami S, Nishitani C, Kunihisa M, Shoda M, Takeuchi M, et al. Complete chloroplast genome sequence of pineapple (Ananas comosus). Tree Genet Genomes. 2015;11:60.

    Article  Google Scholar 

  172. 172.

    Downie SR, Jansen RK. A comparative analysis of whole plastid genomes from the Apiales: expansion and contraction of the inverted repeat, mitochondrial to plastid transfer of DNA, and identification of highly divergent noncoding regions. Syst Botany. 2015;40:336–51.

    Article  Google Scholar 

  173. 173.

    Liu Y, Huo N, Dong L, Wang Y, Zhang S, Young HA, et al. Complete chloroplast genome sequences of Mongolia medicine Artemisia frigida and phylogenetic relationships with other plants. PLoS One. 2013;8, e57533.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  174. 174.

    Schmitz-Linneweber C, Regel R, Du TG, Hupfer H, Herrmann RG, Maier RM. The plastid chromosome of Atropa belladonna and its comparison with that of Nicotiana tabacum: the role of RNA editing in generating divergence in the process of plant speciation. Mol Biol Evol. 2002;19:1602–12.

    CAS  PubMed  Article  Google Scholar 

  175. 175.

    Hu Z-Y, Hua W, Huang S-M, Wang H-Z. Complete chloroplast genome sequence of rapeseed (Brassica napus L.) and its evolutionary implications. Genet Resour Crop Evol. 2011;58:875–87.

    CAS  Article  Google Scholar 

  176. 176.

    Yang JB, Li DZ, Li HT. Highly effective sequencing whole chloroplast genomes of angiosperms by nine novel universal primer pairs. Mol Ecol Resour. 2014;14:1024–31.

    CAS  PubMed  Article  Google Scholar 

  177. 177.

    Oh H, Seo B, Lee S, Ahn D-H, Jo E, Park J-K, Min G-S. Two complete chloroplast genome sequences of Cannabis sativa varieties. Mitochondrial DNA. 2016;27:2835–7.

    PubMed  Article  CAS  Google Scholar 

  178. 178.

    Jo YD, Park J, Kim J, Song W, Hur CG, Lee YH, Kang BC. Complete sequencing and comparative analyses of the pepper (Capsicum annuum L.) plastome revealed high frequency of tandem repeats and large insertion/deletions on pepper plastome. Plant Cell Rep. 2011;30:217–29.

    CAS  PubMed  Article  Google Scholar 

  179. 179.

    Ming R, Hou S, Feng Y, Yu Q, Dionne-Laporte A, Saw JH, et al. The draft genome of the transgenic tropical fruit tree papaya (Carica papaya Linnaeus). Nature. 2008;452:991–6.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  180. 180.

    Ku C, Chung WC, Chen LL, Kuo CH. The complete plastid genome sequence of Madagascar periwinkle Catharanthus roseus (L.) G. Don: plastid genome evolution, molecular marker identification, and phylogenetic implications in asterids. PLoS One. 2013;8, e68518.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  181. 181.

    Mariac C, Scarcelli N, Pouzadou J, Barnaud A, Billot C, Faye A, et al. Cost-effective enrichment hybridization capture of chloroplast genomes at deep multiplexing levels for population genetics and phylogeography studies. Mol Ecol Resour. 2014;14:1103–13.

    CAS  PubMed  Article  Google Scholar 

  182. 182.

    Ahmed I, Biggs PJ, Matthews PJ, Collins LJ, Hendy MD, Lockhart PJ. Mutational dynamics of aroid chloroplast genomes. Genome Biol Evol. 2012;4:1316–23.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  183. 183.

    Plader W, Yukawa Y, Sugiura M, Malepszy S. The complete structure of the cucumber (Cucumis sativus L.) chloroplast genome: its composition and comparative analysis. Cell Mol Biol Lett. 2007;12:584–94.

    CAS  PubMed  Article  Google Scholar 

  184. 184.

    Barrett CF, Specht CD, Leebens-Mack J, Stevenson DW, Zomlefer WB, Davis JI. Resolving ancient radiations: can complete plastid gene sets elucidate deep relationships among the tropical gingers (Zingiberales)? Ann Bot. 2014;113:119–33.

    PubMed  Article  Google Scholar 

  185. 185.

    Wang B, Han L, Chen C, Wang Z. The complete chloroplast genome sequence of Dieffenbachia seguine (Araceae). Mitochondrial DNA. 2016;27:2913–4.

    PubMed  Google Scholar 

  186. 186.

    Ye CY, Lin Z, Li G, Wang YY, Qiu J, Fu F, Zhang H, et al. Echinochloa chloroplast genomes: insights into the evolution and taxonomic identification of two weedy species. PLoS One. 2014;9, e113657.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  187. 187.

    Wu CS, Lai YT, Lin CP, Wang YN, Chaw SM. Evolution of reduced and compact chloroplast genomes (cpDNAs) in gnetophytes: selection toward a lower-cost strategy. Mol Phylogenet Evol. 2009;52:115–24.

    CAS  PubMed  Article  Google Scholar 

  188. 188.

    Logacheva MD, Samigullin TH, Dhingra A, Penin AA. Comparative chloroplast genomics and phylogenetics of Fagopyrum esculentum ssp. ancestrale--a wild ancestor of cultivated buckwheat. BMC Plant Biol. 2008;8:59.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  189. 189.

    Cahoon AB, Sharpe RM, Mysayphonh C, Thompson EJ, Ward AD, Lin A. The complete chloroplast genome of tall fescue (Lolium arundinaceum; Poaceae) and comparison of whole plastomes from the family Poaceae. Am J Bot. 2010;97:49–58.

    CAS  PubMed  Article  Google Scholar 

  190. 190.

    Shulaev V, Sargent DJ, Crowhurst RN, Mockler TC, Folkerts O, Delcher AL, et al. The genome of woodland strawberry (Fragaria vesca). Nat Genet. 2011;43:109–16.

    CAS  PubMed  Article  Google Scholar 

  191. 191.

    Dempewolf H, Kane NC, Ostevik KL, Geleta M, Barker MS, Lai Z, et al. Establishing genomic tools and resources for Guizotia abyssinica (L.f.) Cass.–the development of a library of expressed sequence tags, microsatellite loci, and the sequencing of its chloroplast genome. Mol Ecol Resour. 2010;10:1048–58.

    CAS  PubMed  Article  Google Scholar 

  192. 192.

    Timme RE, Kuehl JV, Boore JL, Jansen RK. A comparative analysis of the Lactuca and Helianthus (Asteraceae) plastid genomes: identification of divergent regions and categorization of shared repeats. Am J Bot. 2007;94:302–12.

    CAS  PubMed  Article  Google Scholar 

  193. 193.

    Barrett CF, Davis JI, Leebens‐Mack J, Conran JG, Stevenson DW. Plastid genomes and deep relationships among the commelinid monocot angiosperms. Cladistics. 2013;29:65–87.

    Article  Google Scholar 

  194. 194.

    Sanchez-Puerta MV, Abbona CC. The chloroplast genome of Hyoscyamus niger and a phylogenetic study of the tribe Hyoscyameae (Solanaceae). PLoS One. 2014;9, e98353.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  195. 195.

    Yan L, Lai X, Li X, Wei C, Tan X, Zhang Y. Analyses of the complete genome and gene expression of chloroplast of sweet potato (Ipomoea batata). PLoS One. 2015;10, e0124083.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  196. 196.

    McNeal JR, Kuehl JV, Boore JL, de Pamphilis CW. Complete plastid genome sequences suggest strong selection for retention of photosynthetic genes in the parasitic plant genus Cuscuta. BMC Plant Biol. 2007;7:57.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  197. 197.

    Kanamoto H, Yamashita A, Asao H, Okumura S, Takase H, Hattori M, et al. Efficient and stable transformation of Lactuca sativa L. cv. Cisco (lettuce) plastids. Transgenic Res. 2006;15:205–17.

    CAS  PubMed  Article  Google Scholar 

  198. 198.

    Mennes CB, Lam VK, Rudall PJ, Lyon SP, Graham SW, Smets EF, Merckx VS. Ancient Gondwana break‐up explains the distribution of the mycoheterotrophic family Corsiaceae (Liliales). J Biogeogr. 2015;42:1123–36.

    Article  Google Scholar 

  199. 199.

    Hand ML, Spangenberg GC, Forster JW, Cogan NO. Plastome sequence determination and comparative analysis for members of the Lolium-Festuca grass species complex. G3 (Bethsada). 2013;3:607–16.

    CAS  Article  Google Scholar 

  200. 200.

    Kato T, Kaneko T, Sato S, Nakamura Y, Tabata S. Complete structure of the chloroplast genome of a legume, Lotus japonicus. DNA Res. 2000;7:323–30.

    CAS  PubMed  Article  Google Scholar 

  201. 201.

    Raubeson LA, Peery R, Chumley TW, Dziubek C, Fourcade HM, Boore JL, Jansen RK. Comparative chloroplast genomics: analyses including new sequences from the angiosperms Nuphar advena and Ranunculus macranthus. BMC Genomics. 2007;8:174.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  202. 202.

    Hiratsuka J, Shimada H, Whittier R, Ishibashi T, Sakamoto M, Mori M, et al. The complete sequence of the rice (Oryza sativa) chloroplast genome: intermolecular recombination between distinct tRNA genes accounts for a major plastid DNA inversion during the evolution of the cereals. Mol Gen Genet. 1989;217:185–94.

    CAS  PubMed  Article  Google Scholar 

  203. 203.

    Kim KJ, Lee HL. Complete chloroplast genome sequences from Korean ginseng (Panax schinseng Nees) and comparative analysis of sequence evolution among 17 vascular plants. DNA Res. 2004;11:247–61.

    CAS  PubMed  Article  Google Scholar 

  204. 204.

    Young HA, Lanzatella CL, Sarath G, Tobias CM. Chloroplast genome variation in upland and lowland switchgrass. PLoS One. 2011;6, e23980.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  205. 205.

    Kumar S, Hahn FM, McMahan CM, Cornish K, Whalen MC. Comparative analysis of the complete sequence of the plastid genome of Parthenium argentatum and identification of DNA barcodes to differentiate Parthenium species and lines. BMC Plant Biol. 2009;9:131.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  206. 206.

    Chumley TW, Palmer JD, Mower JP, Fourcade HM, Calie PJ, Boore JL, Jansen RK. The complete chloroplast genome sequence of Pelargonium x hortorum: organization and evolution of the largest and most highly rearranged chloroplast genome of land plants. Mol Biol Evol. 2006;23:2175–90.

    CAS  PubMed  Article  Google Scholar 

  207. 207.

    Jeong YM, Chung WH, Mun JH, Kim N, Yu HJ. De novo assembly and characterization of the complete chloroplast genome of radish (Raphanus sativus L.). Gene. 2014;551:39–48.

    CAS  PubMed  Article  Google Scholar 

  208. 208.

    Rivarola M, Foster JT, Chan AP, Williams AL, Rice DW, Liu X, et al. Castor bean organelle genome sequencing and worldwide genetic diversity analysis. PLoS One. 2011;6, e21743.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  209. 209.

    Calsa Junior T, Carraro DM, Benatti MR, Barbosa AC, Kitajima JP, Carrer H. Structural features and transcript-editing analysis of sugarcane (Saccharum officinarum L.) chloroplast genome. Curr Genet. 2004;46:366–73.

    CAS  PubMed  Article  Google Scholar 

  210. 210.

    Qian J, Song J, Gao H, Zhu Y, Xu J, Pang X, et al. The complete chloroplast genome sequence of the medicinal plant Salvia miltiorrhiza. PLoS One. 2013;8, e57607.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  211. 211.

    Yi DK, Kim KJ. Complete chloroplast genome sequences of important oilseed crop Sesamum indicum L. PLoS One. 2012;7, e35872.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  212. 212.

    Chung HJ, Jung JD, Park HW, Kim JH, Cha HW, Min SR, et al. The complete chloroplast genome sequences of Solanum tuberosum and comparative analysis with Solanaceae species identified the presence of a 241-bp deletion in cultivated potato chloroplast DNA sequence. Plant Cell Rep. 2006;25:1369–79.

    CAS  PubMed  Article  Google Scholar 

  213. 213.

    Schmitz-Linneweber C, Maier RM, Alcaraz JP, Cottet A, Herrmann RG, Mache R. The plastid chromosome of spinach (Spinacia oleracea): complete nucleotide sequence and gene organization. Plant Mol Biol. 2001;45:307–15.

    CAS  PubMed  Article  Google Scholar 

  214. 214.

    Yi DK, Yang JC, So S, Joo M, Kim DK, Shin CH, et al. The complete plastid genome sequence of Abies koreana (Pinaceae: Abietoideae). Mitochondrial DNA. 2016;27:2351–3.

    PubMed  Google Scholar 

  215. 215.

    Yao X, Tang P, Li Z, Li D, Liu Y, Huang H. The first complete chloroplast genome sequences in Actinidiaceae: genome structure and comparative analysis. PLoS One. 2015;10, e0129347.

    PubMed  PubMed Central  Article  Google Scholar 

  216. 216.

    Hsu CY, Wu CS, Chaw SM. Ancient nuclear plastid DNA in the yew family (Taxaceae). Genome Biol Evol. 2014;6:2111–21.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  217. 217.

    Ruhsam M, Rai HS, Mathews S, Ross TG, Graham SW, Raubeson LA, et al. Does complete plastid genome sequencing improve species discrimination and phylogenetic resolution in Araucaria? Mol Ecol Resour. 2015;15:1067–78.

    CAS  PubMed  Article  Google Scholar 

  218. 218.

    Ma J, Yang B, Zhu W, Sun L, Tian J, Wang X. The complete chloroplast genome sequence of Mahonia bealei (Berberidaceae) reveals a significant expansion of the inverted repeat and phylogenetic relationship with other angiosperms. Gene. 2013;528:120–31.

    CAS  PubMed  Article  Google Scholar 

  219. 219.

    Hansen DR, Dastidar SG, Cai Z, Penaflor C, Kuehl JV, Boore JL, Jansen RK. Phylogenetic and evolutionary implications of complete chloroplast genome sequences of four early-diverging angiosperms: Buxus (Buxaceae), Chloranthus (Chloranthaceae), Dioscorea (Dioscoreaceae), and Illicium (Schisandraceae). Mol Phylogenet Evol. 2007;45:547–63.

    CAS  PubMed  Article  Google Scholar 

  220. 220.

    Wu CS, Chaw SM. Highly rearranged and size-variable chloroplast genomes in conifers II clade (cupressophytes): evolution towards shorter intergenic spacers. Plant Biotechnol J. 2014;12:344–53.

    CAS  PubMed  Article  Google Scholar 

  221. 221.

    Shi C, Liu Y, Huang H, Xia EH, Zhang HB, Gao LZ. Contradiction between plastid gene transcription and function due to complex posttranscriptional splicing: an exemplary study of ycf15 function and evolution in angiosperms. PLoS One. 2013;8, e59620.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  222. 222.

    Huang H, Shi C, Liu Y, Mao SY, Gao LZ. Thirteen Camellia chloroplast genome sequences determined by high-throughput sequencing: genome structure and phylogenetic relationships. BMC Evol Biol. 2014;14:151.

    PubMed  PubMed Central  Article  Google Scholar 

  223. 223.

    Lin CP, Huang JP, Wu CS, Hsu CY, Chaw SM. Comparative chloroplast genomics reveals the evolution of Pinaceae genera and subfamilies. Genome Biol Evol. 2010;2:504–17.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  224. 224.

    Wu CS, Wang YN, Hsu CY, Lin CP, Chaw SM. Loss of different inverted repeat copies from the chloroplast genomes of Pinaceae and cupressophytes and influence of heterotachy on the evaluation of gymnosperm phylogeny. Genome Biol Evol. 2011;3:1284–95.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  225. 225.

    Male PJ, Bardon L, Besnard G, Coissac E, Delsuc F, Engel J, et al. Genome skimming by shotgun sequencing helps resolve the phylogeny of a pantropical tree family. Mol Ecol Resour. 2014;14:966–75.

    CAS  PubMed  Google Scholar 

  226. 226.

    Huang YY, Matzke AJ, Matzke M. Complete sequence and comparative analysis of the chloroplast genome of coconut palm (Cocos nucifera). PLoS One. 2013;8, e74736.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  227. 227.

    Bayly MJ, Rigault P, Spokevicius A, Ladiges PY, Ades PK, Anderson C, et al. Chloroplast genome analysis of Australian eucalypts--Eucalyptus, Corymbia, Angophora, Allosyncarpia and Stockwellia (Myrtaceae). Mol Phylogenet Evol. 2013;69:704–16.

    CAS  PubMed  Article  Google Scholar 

  228. 228.

    Hirao T, Watanabe A, Kurita M, Kondo T, Takata K. Complete nucleotide sequence of the Cryptomeria japonica D. Don. chloroplast genome and comparative chloroplast genomics: diversified genomic structure of coniferous species. BMC Plant Biol. 2008;8:70.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  229. 229.

    Uthaipaisanwong P, Chanprasert J, Shearman JR, Sangsrakru D, Yoocha T, Jomchai N, et al. Characterization of the chloroplast genome sequence of oil palm (Elaeis guineensis Jacq.). Gene. 2012;500:172–80.

    CAS  PubMed  Article  Google Scholar 

  230. 230.

    Steane D, Jones R, Vaillancourt R. A set of chloroplast microsatellite primers for Eucalyptus (Myrtaceae). Mol Ecol Notes. 2005;5:538–41.

    CAS  Article  Google Scholar 

  231. 231.

    Tangphatsornruang S, Uthaipaisanwong P, Sangsrakru D, Chanprasert J, Yoocha T, Jomchai N, Tragoonrung S. Characterization of the complete chloroplast genome of Hevea brasiliensis reveals genome rearrangement, RNA editing sites and phylogenetic relationships. Gene. 2011;475:104–12.

    CAS  PubMed  Article  Google Scholar 

  232. 232.

    Lee HL, Jansen RK, Chumley TW, Kim KJ. Gene relocations within chloroplast genomes of Jasminum and Menodora (Oleaceae) are due to multiple, overlapping inversions. Mol Biol Evol. 2007;24:1161–80.

    CAS  PubMed  Article  Google Scholar 

  233. 233.

    Asif MH, Mantri SS, Sharma A, Srivastava A, Trivedi I, Gupta P, et al. Complete sequence and organisation of the Jatropha curcas (Euphorbiaceae) chloroplast genome. Tree Genet Genomes. 2010;6:941–52.

    Article  Google Scholar 

  234. 234.

    Guo W, Grewe F, Cobo-Clark A, Fan W, Duan Z, Adams RP, et al. Predominant and substoichiometric isomers of the plastid genome coexist within Juniperus plants and have shifted multiple times during cupressophyte evolution. Genome Biol Evol. 2014;6:580–90.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  235. 235.

    Cai Z, Penaflor C, Kuehl JV, Leebens-Mack J, Carlson JE, de Pamphilis CW, et al. Complete plastid genome sequences of Drimys, Liriodendron, and Piper: implications for the phylogenetic relationships of magnoliids. BMC Evol Biol. 2006;6:77.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  236. 236.

    Chen J, Hao Z, Xu H, Yang L, Liu G, Sheng Y, et al. The complete chloroplast genome sequence of the relict woody plant Metasequoia glyptostroboides Hu et Cheng. Front Plant Sci. 2015;6:447.

    PubMed  PubMed Central  Google Scholar 

  237. 237.

    Ravi V, Khurana JP, Tyagi AK, Khurana P. The chloroplast genome of mulberry: complete nucleotide sequence, gene organization and comparative analysis. Tree Genet Genomes. 2006;3:49–59.

    Article  Google Scholar 

  238. 238.

    Straub SC, Moore MJ, Soltis PS, Soltis DE, Liston A, Livshultz T. Phylogenetic signal detection from an ancient rapid radiation: effects of noise reduction, long-branch attraction, and model selection in crown clade Apocynaceae. Mol Phylogenet Evol. 2014;80:169–85.

    PubMed  Article  Google Scholar 

  239. 239.

    Besnard G, Hernández P, Khadari B, Dorado G, Savolainen V. Genomic profiling of plastid DNA variation in the Mediterranean olive tree. BMC Plant Biol. 2011;11:80.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  240. 240.

    Yang M, Zhang X, Liu G, Yin Y, Chen K, Yun Q, et al. The complete chloroplast genome sequence of date palm (Phoenix dactylifera L.). PLoS One. 2010;5, e12762.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  241. 241.

    Fang MF, Wang YJ, Zu YM, Dong WL, Wang RN, Deng TT, Li ZH. The complete chloroplast genome of the Taiwan red pine Pinus taiwanensis (Pinaceae). Mitochondrial DNA. 2016;27:2732–3.

    PubMed  Article  CAS  Google Scholar 

  242. 242.

    Vieira Ldo N, Faoro H, Rogalski M, Fraga HP, Cardoso RL, de Souza EM, et al. The complete chloroplast genome sequence of Podocarpus lambertii: genome structure, evolutionary aspects, gene content and SSR detection. PLoS One. 2014;9, e90618.

    PubMed  Article  Google Scholar 

  243. 243.

    Okumura S, Sawada M, Park YW, Hayashi T, Shimamura M, Takase H, Tomizawa K. Transformation of poplar (Populus alba) plastids and expression of foreign proteins in tree chloroplasts. Transgenic Res. 2006;15:637–46.

    CAS  PubMed  Article  Google Scholar 

  244. 244.

    Wang S, Shi C, Gao LZ. Plastid genome sequence of a wild woody oil species, Prinsepia utilis, provides insights into evolutionary and mutational patterns of Rosaceae chloroplast genomes. PLoS One. 2013;8, e73946.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  245. 245.

    Terakami S, Matsumura Y, Kurita K, Kanamori H, Katayose Y, Yamamoto T, Katayama H. Complete sequence of the chloroplast genome from pear (Pyrus pyrifolia): genome structure and comparative analysis. Tree Genet Genomes. 2012;8:841–54.

    Article  Google Scholar 

  246. 246.

    Alexander LW, Woeste KE. Pyrosequencing of the northern red oak (Quercus rubra L.) chloroplast genome reveals high quality polymorphisms for population management. Tree Genet Genomes. 2014;10:803–12.

    Article  Google Scholar 

  247. 247.

    Yang B, Li M, Ma J, Fu Z, Xu X, Chen Q, et al. The complete chloroplast genome sequence of Sapindus mukorossi. Mitochondrial DNA. 2016;27:1825–6.

    PubMed  Google Scholar 

  248. 248.

    Fajardo D, Senalik D, Ames M, Zhu H, Steffan SA, Harbut R, et al. Complete plastid genome sequence of Vaccinium macrocarpon: structure, gene content, and rearrangements revealed by next generation sequencing. Tree Genet Genomes. 2013;9:489–98.

    Article  Google Scholar 

  249. 249.

    Yap JY, Rohner T, Greenfield A, Van Der Merwe M, McPherson H, Glenn W, et al. Complete chloroplast genome of the wollemi pine (Wollemia nobilis): structure and evolution. PLoS One. 2015;10, e0128126.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  250. 250.

    Shaw J, Lickey EB, Beck JT, Farmer SB, Liu W, Miller J, et al. The tortoise and the hare II: relative utility of 21 noncoding chloroplast DNA sequences for phylogenetic analysis. Am J Bot. 2005;92:142–66.

    CAS  PubMed  Article  Google Scholar 

  251. 251.

    Ruhlman TA, Rajasekaran K, Cary JW. Expression of chloroperoxidase from Pseudomonas pyrrocinia in tobacco plastids for fungal resistance. Plant Sci. 2014;228:98–106.

    CAS  PubMed  Article  Google Scholar 

  252. 252.

    Chakrabarti SK, Lutz KA, Lertwiriyawong B, Svab Z, Maliga P. Expression of the cry9Aa2 B.t. gene in tobacco chloroplasts confers resistance to potato tuber moth. Transgenic Res. 2006;15:481–8.

    CAS  PubMed  Article  Google Scholar 

  253. 253.

    DeGray G, Rajasekaran K, Smith F, Sanford J, Daniell H. Expression of an antimicrobial peptide via the chloroplast genome to control phytopathogenic bacteria and fungi. Plant Physiol. 2001;127:852–62.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  254. 254.

    Chen PJ, Senthilkumar R, Jane WN, He Y, Tian Z, Yeh KW. Transplastomic Nicotiana benthamiana plants expressing multiple defence genes encoding protease inhibitors and chitinase display broad-spectrum resistance against insects, pathogens and abiotic stresses. Plant Biotechnol J. 2014;12:503–15.

    CAS  PubMed  Article  Google Scholar 

  255. 255.

    Wang YP, Wei ZY, Zhang YY, Lin CJ, Zhong XF, Wang YL, et al. Chloroplast-expressed MSI-99 in tobacco improves disease resistance and displays inhibitory effect against rice blast fungus. Int J Mol Sci. 2015;16:4628–41.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  256. 256.

    McBride KE, Svab Z, Schaaf DJ, Hogan PS, Stalker DM, Maliga P. Amplification of a chimeric Bacillus gene in chloroplasts leads to an extraordinary level of an insecticidal protein in tobacco. Biotechnology (N Y). 1995;13:362–5.

    CAS  Article  Google Scholar 

  257. 257.

    Kota M, Daniell H, Varma S, Garczynski SF, Gould F, Moar WJ. Overexpression of the Bacillus thuringiensis (Bt) Cry2Aa2 protein in chloroplasts confers resistance to plants against susceptible and Bt-resistant insects. Proc Natl Acad Sci U S A. 1999;96:1840–5.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  258. 258.

    Lee SB, Kwon HB, Kwon SJ, Park SC, Jeong MJ, Han SE, et al. Accumulation of trehalose within transgenic chloroplasts confers drought tolerance. Mol Breeding. 2003;11:1–13.

    CAS  Article  Google Scholar 

  259. 259.

    Ruiz ON, Hussein HS, Terry N, Daniell H. Phytoremediation of organomercurial compounds via chloroplast genetic engineering. Plant Physiol. 2003;132:1344–52.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  260. 260.

    Ruiz ON, Alvarez D, Torres C, Roman L, Daniell H. Metallothionein expression in chloroplasts enhances mercury accumulation and phytoremediation capability. Plant Biotechnol J. 2011;9:609–17.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  261. 261.

    Lutz KA, Knapp JE, Maliga P. Expression of bar in the plastid genome confers herbicide resistance. Plant Physiol. 2001;125:1585–90.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  262. 262.

    Ye GN, Hajdukiewicz PT, Broyles D, Rodriguez D, Xu CW, Nehra N, Staub JM. Plastid-expressed 5-enolpyruvylshikimate-3-phosphate synthase genes provide high level glyphosate tolerance in tobacco. Plant J. 2001;25:261–70.

    CAS  PubMed  Article  Google Scholar 

  263. 263.

    Shimizu M, Goto M, Hanai M, Shimizu T, Izawa N, Kanamoto H, et al. Selectable tolerance to herbicides by mutated acetolactate synthase genes integrated into the chloroplast genome of tobacco. Plant Physiol. 2008;147:1976–83.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  264. 264.

    Iamtham S, Day A. Removal of antibiotic resistance genes from transgenic tobacco plastids. Nat Biotechnol. 2000;18:1172–6.

    CAS  PubMed  Article  Google Scholar 

  265. 265.

    Dufourmantel N, Dubald M, Matringe M, Canard H, Garcon F, Job C, et al. Generation and characterization of soybean and marker-free tobacco plastid transformants over-expressing a bacterial 4-hydroxyphenylpyruvate dioxygenase which provides strong herbicide tolerance. Plant Biotechnol J. 2007;5:118–33.

    CAS  PubMed  Article  Google Scholar 

  266. 266.

    Fouad WM, Altpeter F. Transplastomic expression of bacterial L-aspartate-alpha-decarboxylase enhances photosynthesis and biomass production in response to high temperature stress. Transgenic Res. 2009;18:707–18.

    CAS  PubMed  Article  Google Scholar 

  267. 267.

    Lu Y, Rijzaani H, Karcher D, Ruf S, Bock R. Efficient metabolic pathway engineering in transgenic tobacco and tomato plastids with synthetic multigene operons. Proc Natl Acad Sci U S A. 2013;110:E623–32.

    PubMed  PubMed Central  Article  Google Scholar 

  268. 268.

    Ruiz ON, Daniell H. Engineering cytoplasmic male sterility via the chloroplast genome by expression of beta-ketothiolase. Plant Physiol. 2005;138:1232–46.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  269. 269.

    Yabuta Y, Tanaka H, Yoshimura S, Suzuki A, Tamoi M, Maruta T, Shigeoka S. Improvement of vitamin E quality and quantity in tobacco and lettuce by chloroplast genetic engineering. Transgenic Res. 2013;22:391–402.

    CAS  PubMed  Article  Google Scholar 

  270. 270.

    Harada H, Maoka T, Osawa A, Hattan J, Kanamoto H, Shindo K, et al. Construction of transplastomic lettuce (Lactuca sativa) dominantly producing astaxanthin fatty acid esters and detailed chemical analysis of generated carotenoids. Transgenic Res. 2014;23:303–15.

    CAS  PubMed  Article  Google Scholar 

  271. 271.

    Pengelly JJ, Forster B, von Caemmerer S, Badger MR, Price GD, Whitney SM. Transplastomic integration of a cyanobacterial bicarbonate transporter into tobacco chloroplasts. J Exp Bot. 2014;65:3071–80.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  272. 272.

    Sanz-Barrio R, Corral-Martinez P, Ancin M, Segui-Simarro JM, Farran I. Overexpression of plastidial thioredoxin f leads to enhanced starch accumulation in tobacco leaves. Plant Biotechnol J. 2013;11:618–27.

    CAS  PubMed  Article  Google Scholar 

  273. 273.

    Elghabi Z, Karcher D, Zhou F, Ruf S, Bock R. Optimization of the expression of the HIV fusion inhibitor cyanovirin-N from the tobacco plastid genome. Plant Biotechnol J. 2011;9:599–608.

    CAS  PubMed  Article  Google Scholar 

  274. 274.

    Agrawal P, Verma D, Daniell H. Expression of Trichoderma reesei beta-mannanase in tobacco chloroplasts and its utilization in lignocellulosic woody biomass hydrolysis. PLoS One. 2011;6, e29302.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  275. 275.

    Verma D, Jin S, Kanagaraj A, Singh ND, Daniel J, Kolattukudy PE, et al. Expression of fungal cutinase and swollenin in tobacco chloroplasts reveals novel enzyme functions and/or substrates. PLoS One. 2013;8, e57187.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  276. 276.

    Saxena B, Subramaniyan M, Malhotra K, Bhavesh NS, Potlakayala SD, Kumar S. Metabolic engineering of chloroplasts for artemisinic acid biosynthesis and impact on plant growth. J Biosci. 2014;39:33–41.

    CAS  PubMed  Article  Google Scholar 

  277. 277.

    Yu LX, Gray BN, Rutzke CJ, Walker LP, Wilson DB, Hanson MR. Expression of thermostable microbial cellulases in the chloroplasts of nicotine-free tobacco. J Biotechnol. 2007;131:362–9.

    CAS  PubMed  Article  Google Scholar 

  278. 278.

    Petersen K, Bock R. High-level expression of a suite of thermostable cell wall-degrading enzymes from the chloroplast genome. Plant Mol Biol. 2011;76:311–21.

    CAS  PubMed  Article  Google Scholar 

  279. 279.

    Arai Y, Shikanai T, Doi Y, Yoshida S, Yamaguchi I, Nakashita H. Production of polyhydroxybutyrate by polycistronic expression of bacterial genes in tobacco plastid. Plant Cell Physiol. 2004;45:1176–84.

    CAS  PubMed  Article  Google Scholar 

  280. 280.

    Hasunuma T, Miyazawa SI, Yoshimura S, Shinzaki Y, Tomizawa KI, Shindo K, et al. Biosynthesis of astaxanthin in tobacco leaves by transplastomic engineering. Plant J. 2008;55:857–68.

    CAS  PubMed  Article  Google Scholar 

  281. 281.

    Nakahira Y, Ishikawa K, Tanaka K, Tozawa Y, Shiina T. Overproduction of hyperthermostable beta-1,4-endoglucanase from the archaeon Pyrococcus horikoshii by tobacco chloroplast engineering. Biosci Biotechnol Biochem. 2013;77:2140–3.

    CAS  PubMed  Article  Google Scholar 

  282. 282.

    Sherman A, Su J, Lin S, Wang X, Herzog RW, Daniell H. Suppression of inhibitor formation against FVIII in a murine model of hemophilia A by oral delivery of antigens bioencapsulated in plant cells. Blood. 2014;124:1659–68.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  283. 283.

    Sanz-Barrio R, Fernandez-San Millan A, Corral-Martinez P, Segui-Simarro JM, Farran I. Tobacco plastidial thioredoxins as modulators of recombinant protein production in transgenic chloroplasts. Plant Biotechnol J. 2011;9:639–50.

    CAS  PubMed  Article  Google Scholar 

  284. 284.

    Farran I, McCarthy-Suarez I, Rio-Manterola F, Mansilla C, Lasarte JJ, Mingo-Castel AM. The vaccine adjuvant extra domain A from fibronectin retains its proinflammatory properties when expressed in tobacco chloroplasts. Planta. 2010;231:977–90.

    CAS  PubMed  Article  Google Scholar 

  285. 285.

    Boyhan D, Daniell H. Low-cost production of proinsulin in tobacco and lettuce chloroplasts for injectable or oral delivery of functional insulin and C-peptide. Plant Biotechnol J. 2011;9:585–98.

    CAS  PubMed  Article  Google Scholar 

  286. 286.

    Fernandez-San Millan A, Mingo-Castel A, Miller M, Daniell H. A chloroplast transgenic approach to hyper-express and purify human serum albumin, a protein highly susceptible to proteolytic degradation. Plant Biotechnol J. 2003;1:71–9.

    CAS  PubMed  Article  Google Scholar 

  287. 287.

    Daniell H, Ruiz G, Denes B, Sandberg L, Langridge W. Optimization of codon composition and regulatory elements for expression of human insulin like growth factor-1 in transgenic chloroplasts and evaluation of structural identity and function. BMC Biotechnol. 2009;9:33.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  288. 288.

    Wang X, Su J, Sherman A, Rogers GL, Liao G, Hoffman BE, et al. Plant-based oral tolerance to hemophilia therapy employs a complex immune regulatory response including LAP+CD4+ T cells. Blood. 2015;125:2418–27.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  289. 289.

    Su J, Sherman A, Doerfler PA, Byrne BJ, Herzog RW, Daniell H. Oral delivery of Acid Alpha Glucosidase epitopes expressed in plant chloroplasts suppresses antibody formation in treatment of Pompe mice. Plant Biotechnol J. 2015;13:1023–32.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  290. 290.

    Youm JW, Jeon JH, Kim H, Min SR, Kim MS, Joung H, et al. High-level expression of a human beta-site APP cleaving enzyme in transgenic tobacco chloroplasts and its immunogenicity in mice. Transgenic Res. 2010;19:1099–108.

    CAS  PubMed  Article  Google Scholar 

  291. 291.

    Arlen PA, Falconer R, Cherukumilli S, Cole A, Cole AM, Oishi KK, Daniell H. Field production and functional evaluation of chloroplast-derived interferon-alpha2b. Plant Biotechnol J. 2007;5:511–25.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  292. 292.

    Ruhlman T, Ahangari R, Devine A, Samsam M, Daniell H. Expression of cholera toxin B-proinsulin fusion protein in lettuce and tobacco chloroplasts--oral administration protects against development of insulitis in non-obese diabetic mice. Plant Biotechnol J. 2007;5:495–510.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  293. 293.

    Leelavathi S, Reddy VS. Chloroplast expression of His-tagged GUS-fusions: a general strategy to overproduce and purify foreign proteins using transplastomic plants as bioreactors. Mol Breeding. 2003;11:49–58.

    CAS  Article  Google Scholar 

  294. 294.

    Lim S, Ashida H, Watanabe R, Inai K, Kim YS, Mukougawa K, et al. Production of biologically active human thioredoxin 1 protein in lettuce chloroplasts. Plant Mol Biol. 2011;76:335–44.

    CAS  PubMed  Article  Google Scholar 

  295. 295.

    Nadai M, Bally J, Vitel M, Job C, Tissot G, Botterman J, Dubald M. High-level expression of active human alpha1-antitrypsin in transgenic tobacco chloroplasts. Transgenic Res. 2009;18:173–83.

    CAS  PubMed  Article  Google Scholar 

  296. 296.

    Gisby MF, Mellors P, Madesis P, Ellin M, Laverty H, O'Kane S, et al. A synthetic gene increases TGF beta 3 accumulation by 75-fold in tobacco chloroplasts enabling rapid purification and folding into a biologically active molecule. Plant Biotechnol J. 2011;9:618–28.

    CAS  PubMed  Article  Google Scholar 

  297. 297.

    Farran I, Rio-Manterola F, Iniguez M, Garate S, Prieto J, Mingo-Castel AM. High-density seedling expression system for the production of bioactive human cardiotrophin-1, a potential therapeutic cytokine, in transgenic tobacco chloroplasts. Plant Biotechnol J. 2008;6:516–27.

    CAS  PubMed  Article  Google Scholar 

  298. 298.

    Staub JM, Garcia B, Graves J, Hajdukiewicz PT, Hunter P, Nehra N, et al. High-yield production of a human therapeutic protein in tobacco chloroplasts. Nat Biotechnol. 2000;18:333–8.

    CAS  PubMed  Article  Google Scholar 

  299. 299.

    Oey M, Lohse M, Scharff LB, Kreikemeyer B, Bock R. Plastid production of protein antibiotics against pneumonia via a new strategy for high-level expression of antimicrobial proteins. Proc Natl Acad Sci U S A. 2009;106:6579–84.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  300. 300.

    Koya V, Moayeri M, Leppla SH, Daniell H. Plant-based vaccine: mice immunized with chloroplast-derived anthrax protective antigen survive anthrax lethal toxin challenge. Infect Immun. 2005;73:8266–74.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  301. 301.

    Watson J, Koya V, Leppla SH, Daniell H. Expression of Bacillus anthracis protective antigen in transgenic chloroplasts of tobacco, a non-food/feed crop. Vaccine. 2004;22:4374–84.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  302. 302.

    Millan AFS, Ortigosa SM, Hervas-Stubbs S, Corral-Martinez P, Segui-Simarro JM, Gaetan J, et al. Human papillomavirus L1 protein expressed in tobacco chloroplasts self-assembles into virus-like particles that are highly immunogenic. Plant Biotechnol J. 2008;6:427–41.

    Article  CAS  Google Scholar 

  303. 303.

    Yacono MD, Farran I, Becher ML, Sander V, Sanchez VR, Martin V, et al. A chloroplast-derived Toxoplasma gondii GRA4 antigen used as an oral vaccine protects against toxoplasmosis in mice. Plant Biotechnol J. 2012;10:1136–44.

    CAS  Article  Google Scholar 

  304. 304.

    Kolotilin I, Kaldis A, Devriendt B, Joensuu J, Cox E, Menassa R. Production of a subunit vaccine candidate against porcine post-weaning diarrhea in high-biomass transplastomic tobacco. Plos One. 2012;7, e42405.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  305. 305.

    Arlen PA, Singleton M, Adamovicz JJ, Ding Y, Davoodi-Semiromi A, Daniell H. Effective plague vaccination via oral delivery of plant cells expressing F1-V antigens in chloroplasts. Infect Immun. 2008;76:3640–50.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  306. 306.

    Molina A, Hervas-Stubbs S, Daniell H, Mingo-Castel AM, Veramendi J. High-yield expression of a viral peptide animal vaccine in transgenic tobacco chloroplasts. Plant Biotechnol J. 2004;2:141–53.

    CAS  PubMed  Article  Google Scholar 

  307. 307.

    Lentz EM, Mozgovoj MV, Bellido D, Santos MJD, Wigdorovitz A, Bravo-Almonacid FF. VP8*antigen produced in tobacco transplastomic plants confers protection against bovine rotavirus infection in a suckling mouse model. J Biotechnol. 2011;156:100–7.

    CAS  PubMed  Article  Google Scholar 

  308. 308.

    Daniell H, Lee SB, Panchal T, Wiebe PO. Expression of the native cholera toxin B subunit gene and assembly as functional oligomers in transgenic tobacco chloroplasts. J Mol Biol. 2001;311:1001–9.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  309. 309.

    Kang TJ, Loc NH, Jang MO, Jang YS, Kim YS, Seo JE, Yang MS. Expression of the B subunit of E. coli heat-labile enterotoxin in the chloroplasts of plants and its characterization. Transgenic Res. 2003;12:683–91.

    CAS  PubMed  Article  Google Scholar 

  310. 310.

    Chebolu S, Daniell H. Stable expression of Gal/GalNAc lectin of Entamoeba histolytica in transgenic chloroplasts and immunogenicity in mice towards vaccine development for amoebiasis. Plant Biotechnol J. 2007;5:230–9.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  311. 311.

    Glenz K, Bouchon B, Stehle T, Wallich R, Simon MM, Warzecha H. Production of a recombinant bacterial lipoprotein in higher plant chloroplasts. Nat Biotechnol. 2006;24:76–7.

    CAS  PubMed  Article  Google Scholar 

  312. 312.

    Rosales-Mendoza S, Alpuche-Solis AG, Soria-Guerra RE, Moreno-Fierros L, Martinez-Gonzalez L, Herrera-Diaz A, Korban SS. Expression of an Escherichia coli antigenic fusion protein comprising the heat labile toxin B subunit and the heat stable toxin, and its assembly as a functional oligomer in transplastomic tobacco plants. Plant J. 2009;57:45–54.

    CAS  PubMed  Article  Google Scholar 

  313. 313.

    Soria-Guerra RE, Alpuche-Solis AG, Rosales-Mendoza S, Moreno-Fierros L, Bendik EM, Martinez-Gonzalez L, Korban SS. Expression of a multi-epitope DPT fusion protein in transplastomic tobacco plants retains both antigenicity and immunogenicity of all three components of the functional oligomer. Planta. 2009;229:1293–302.

    CAS  PubMed  Article  Google Scholar 

  314. 314.

    Rubio-Infante N, Govea-Alonso DO, Alpuche-Solis AG, Garcia-Hernandez AL, Soria-Guerra RE, Paz-Maldonado LMT, et al. A chloroplast-derived C4V3 polypeptide from the human immunodeficiency virus (HIV) is orally immunogenic in mice. Plant Mol Biol. 2012;78:337–49.

    CAS  PubMed  Article  Google Scholar 

  315. 315.

    Waheed MT, Thones N, Muller M, Hassan SW, Gottschamel J, Lossl E, et al. Plastid expression of a double-pentameric vaccine candidate containing human papillomavirus-16 L1 antigen fused with LTB as adjuvant: transplastomic plants show pleiotropic phenotypes. Plant Biotechnol J. 2011;9:651–60.

    CAS  PubMed  Article  Google Scholar 

  316. 316.

    Gonzalez-Rabade N, McGowan EG, Zhou F, McCabe MS, Bock R, Dix PJ, et al. Immunogenicity of chloroplast-derived HIV-1 p24 and a p24-Nef fusion protein following subcutaneous and oral administration in mice. Plant Biotechnol J. 2011;9:629–38.

    CAS  PubMed  Article  Google Scholar 

  317. 317.

    Zhou YX, Lee MYT, Ng JMH, Chye ML, Yip WK, Zee SY, Lam E. A truncated hepatitis E virus ORF2 protein expressed in tobacco plastids is immunogenic in mice. World J Gastroenterol. 2006;12:306–12.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  318. 318.

    Shao HB, He DM, Qian KX, Shen GF, Su ZL. The expression of classical swine fever virus structural protein E2 gene in tobacco chloroplasts for applying chloroplasts as bioreactors. C R Biol. 2008;331:179–84.

    CAS  PubMed  Article  Google Scholar 

  319. 319.

    Tregoning JS, Nixon P, Kuroda H, Svab Z, Clare S, Bowe F, et al. Expression of tetanus toxin Fragment C in tobacco chloroplasts. Nucleic Acids Res. 2003;31:1174–9.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  320. 320.

    Morgenfeld M, Lentz E, Segretin ME, Alfano EF, Bravo-Almonacid F. Translational fusion and redirection to thylakoid lumen as strategies to enhance accumulation of human papillomavirus E7 antigen in tobacco chloroplasts. Mol Biotechnol. 2014;56:1021–31.

    CAS  PubMed  Article  Google Scholar 

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Acknowledgements

We acknowledge valuable contributions from editors of Genome Biology (Drs Dominique Morneau and Ripudaman Bains) to enhance the flow and presentation to non-specialists and reviewers for their critical comments.

Funding

Research from the Daniell Laboratory included in this review was supported by the Bill and Melinda Gates Foundation (OPP1031406), the National Institutes of Health (NIH; R01 HL107904, HL109442, GM 63879, EY 024564), Novo Nordisk, Bayer, and Department of Energy ARPA-E grants to Henry Daniell.

Authors’ contributions

HD and CSL wrote this review. MY assembled Table 2 and Figs. 3 and 4 with guidance from HD. WJC assembled Table 1 and Figs. 1 and 2 with guidance from CSL. All authors read and approved the final manuscript.

Competing interests

Henry Daniell, as a pioneer in the field of chloroplast genetic engineering, has several patents in this field but has no financial conflict of interest to declare. A complete list of published patents is available at the Google Scholar weblink: http://scholar.google.com/citations?user=7sow4jwAAAAJ&hl=en

Author information

Affiliations

  1. Department of Biochemistry, School of Dental Medicine, University of Pennsylvania, South 40th St, Philadelphia, PA, 19104-6030, USA
    • Henry Daniell
    •  & Ming Yu
  2. Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan