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The shocking consequences of hybrid epigenomes
Genome Biologyvolume 17, Article number: 85 (2016)
The Erratum to this article has been published in Genome Biology 2016 17:165
The formation of spontaneous epialleles is poorly understood. A new study describes how the formation of epihybrids can lead to the appearance of novel epialleles.
DNA methylation is perhaps the best-known and best-studied chromatin modification. Occurring primarily at cytosine nucleotides in eukaryotic genomes, methylation is a reversible DNA modification that is linked with epialleles, which are genes that are identical in sequence but differ in methylation status and that are inherited between generations. A recent study by Rigal et al.  published in Proceedings of the National Academy of Sciences U S A reported the discovery of novel epialleles in hybrids that formed from crossing mutants that are defective in the maintenance of DNA methylation with wild-type plants, highlighting a potential mechanism by which natural epialleles arise.
Maintenance of DNA methylation in plants
The flowering plant Arabidopsis thaliana is an ideal model system for studying DNA methylation because it can tolerate extensive genome-wide methylation changes. Unlike in mammals, DNA methylation in A. thaliana regularly occurs at three different sequence contexts: CG, CHG, and CHH (where H is either A, C, or T). In A. thaliana, CG methylation is maintained genome-wide by METHYLTRANSFERASE1 (MET1), whereas CHG and CHH methylation are maintained by CHROMOMETHYLTRANSFERASE3 (CMT3) and CMT2, respectively. De novo DNA methylation at any cytosine context is mostly a product of DOMAINS REARRANGED METHYLTRANSFERASE 2 (DRM2) and occurs as part of the RNA-directed DNA methylation pathway.
Mutations in MET1 in A. thaliana eliminate 99 % of all genome-wide CG methylation, yet these plants still produce viable offspring . This characteristic has led to the creation of epigenetic recombinant inbred lines, or epiRILs. Crossing MET1-deficient individuals with wild-type plants creates progeny that contain mosaic methylomes that have no underlying DNA sequence changes, resulting in numerous epialleles . Epialleles are known to cause widespread phenotypic variation in A. thaliana . epiRILs are important in studying the long-term impacts of differential methylation, but the short-term impacts of epiallele formation remain poorly characterized.
Spontaneous epiallele formation
Rigal et al.  previously reported that the lack of CG and CHG methylation in the large intron of INCREASE IN BONSAI METHYLATION 1 (IBM1) results in impaired IBM1 transcription. IBM1 is a histone lysine demethylase that removes H3K9me2 from actively transcribed gene bodies, and protects genes from CHG methylation. Reduced expression of IBM1 leads to aberrant CHG hypermethylation in thousands of genes . In their recent study, published in PNAS, Rigal et al.  crossed individuals with a defective MET1 gene—which leads to decreased IBM1 intronic CG methylation and decreased expression—with Col-0 wild-type individuals . Unexpectedly, the resulting offspring (termed epihybrids) exhibited further reduction in both IBM1 intronic CHG methylation and mRNA levels at the met1-derived IBM1 allele. This was not due to the effects of the met1 mutation, as determined from several generations of self-pollination. Thus, the decrease in IBM1 intronic CHG methylation is due either to interactions between the different parental epialleles or to large-scale differences in chromatin architecture between the two genomes. Self-pollinating met1 x Col-0 epihybrid plants revealed a Mendelian inheritance pattern for the newly formed IBM1 epiallele in the F2 progeny; one-quarter of the offspring produced low levels of full-length IBM1 transcripts and possessed no methylation in the intron. This particular novel epiallele was verified by reversing the orientation of the cross. It was stably inherited for at least two consecutive generations, indicating that other epigenetic changes found in the epihybrids could be inherited in future generations.
These results provide evidence for an ‘epigenomic shock’, where the segregation of genomes with vastly different chromatin landscapes results in the formation of novel epialleles, which have the potential to alter the expression of protein-coding genes. Similar dynamics involving the crossing of different epigenomic states are likely to occur regularly in the wild, albeit at a smaller scale, and provide a possible mechanism for the creation of novel natural epialleles, as has been observed in crosses between some A. thaliana accessions .
Transposable elements in epihybrids
Rigal et al.  performed whole-genome bisulfite sequencing for both parents and epihybrids to compare methylation changes on a genome-wide scale. F1 epihybrid plants showed a substantial increase in CG methylation at certain transposons (TEs) located in pericentromeric regions (Fig. 1). A notable portion (25 %) of TEs had fully restored CG methylation in epihybrid plants, and full CG restoration was associated with re-establishment of CHG methylation. The restoration of methylation at these TEs indicates that pre-existing properties are present to facilitate this immediate return of methylation. This contrasts sharply with what occurs within genes. Roughly 100 CHG hypermethylated genes were identified in the F1 epihybrids that had no hypermethylation in either parent. Of these genes, 60 % are also CHG hypermethylated in ibm1 mutants, suggesting that deficient IBM1 activity is responsible for this aberrant CHG methylation. However, CG gene-body methylation did not deviate from expected levels (Fig. 1), indicating that regions of active remethylation are concentrated on silencing TEs in pericentromeric regions of the genome.
Most intriguingly, of the nearly 2000 re-activated TEs found in the met1 parent (re-activated by loss of TE-silencing methylation), fewer than 3 % were transcriptionally re-silenced in the F1 epihybrids. This evidence indicates that the addition of CG methylation from the wild-type parent alone is not sufficient to affect the transcription levels of re-activated TEs (Fig. 1). Rigal et al.  propose that the poorly expressed IBM1 allele from the met1 parent, coupled with the re-activation and transdemethylation of transposons, recruits the remaining IBM1 to preferentially remove CHG methylation found in transcriptionally reactivated transposons in F1 epihybrids (Fig. 1).This titration of IBM1 away from genic regions is perhaps responsible for the observed increase in CHG gene-body methylation in F2 plants.
Consequences of epigenome reprogramming
How CHG methylation is initially lost from the met1-derived IBM1 allele is unknown, and will be an important area for future investigations. Other examples of widespread epigenome reprogramming have been observed in recent years when studying mutants that are deficient in some aspect of DNA methylation maintenance. In met1 mutants, H3K9me2 has been observed in H3K27me3-targeted genes, whereas H3K9me2-depleted regions acquire H3K27me3 . In another study using a mutant with a globally hypomethylated genome, known as decrease in DNA methylation1 (ddm1), hypermethylation was observed at non-CG sites. Increased H3K9me2 also occurred at these sites over generational time scales, probably as the result of the misregulation of a feed-forward loop involving CMT3 and the H3K9 lysine methyltransferase KRYPTONITE . Collectively, these results indicate that chromatin landscape incompatibility, as noted in ddm1 and met1 mutants, has the potential to alter crucial associations between histone modifications and DNA methylation, ultimately leading to the creation of altered epigenetic states.
The consequences of large-scale alterations in chromatin landscapes have also been observed in animals. The loss of H4K16ac and H4K20me3 is a hallmark of nearly every human cancer studied to date, while ectopic levels of H3K9ac and H3K4me2 can promote the formation of tumors in multiple tissue types . Numerous mechanisms, including the misexpression of histone lysine methyltransferases such as the H3K9me2 methyltransferase G9a, have been found to promote the metastasis of cancerous cells . By and large, the consequences of altered chromatin landscapes in promoting cancer proliferation remain largely unknown. Understanding the mechanisms and consequences of how genome integrity is disrupted is an important area of investigation that could lead to methods for engineering aberrant chromatin landscape alterations for better crop development and potentially for treating chromatin-based diseases.
- ddm1 :
decrease in DNA methylation1
DOMAINS REARRANGED METHYLTRANSFERASE 2
epigenetic recombinant inbred line
- IBM1 :
INCREASE IN BONSAI METHYLATION 1
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This work was supported by the National Science Foundation (MCB–1402183).
The authors declare that they have no competing interests.
WTJ and RJS contributed equally to the writing of this research highlight. Both authors read and approved the final manuscript.