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Open Access

Erratum to: Genome-wide incorporation dynamics reveal distinct categories of turnover for the histone variant H3.3

  • Daniel C. Kraushaar1,
  • Wenfei Jin1,
  • Alika Maunakea1,
  • Brian Abraham1,
  • Misook Ha2 and
  • Keji Zhao1Email author
Contributed equally
Genome Biology201617:21

Received: 25 January 2016

Accepted: 25 January 2016

Published: 4 February 2016

The original article was published in Genome Biology 2013 14:R121

After the publication of this work [1] an error was noticed in Fig. 1d. In the DAPI columns the same image was used accidentally for the 48 h and 72 h timepoints. The corrected figure is shown below. We apologize for this error.
Fig. 1

A versatile system to study replication-independent nucleosome dynamics in mammals. (a) Schematic of TET-inducible expression system to study H3.3 turnover. CMV, cytomegalovirus; rtTA, reverse tetracycline-controlled transactivator; TRE, tetracycline responsive elements. (b) Western blot showing protein levels of transgenic HA/FLAG-H3.3 compared to endogenous H3.3. HA/FLAG-H3.3 expression 24 hours after DOX addition. The band marked with an asterisk is non-specific. The arrow marks transgenic HA/FLAG-H3.3. (c) Time course western blots of HA/FLAG-H3.3 expression. (d) Bromodeoxyuridine (BrdU) immunostaining of NIH/3 T3 cells treated with DNA polymerase inhibitor aphidicolin and DOX across time points of H3.3 induction. DMSO, dimethylsulfoxide. (e) Cell cycle analysis of cells treated with aphidicolin/DOX. Cells were stained with propidium iodide and analyzed by flow cytometry



Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.

Authors’ Affiliations

Systems Biology Center, National Heart, Lung, and Blood Institute, NIH
Samsung Advanced Institute of Technology, Samsung Electronics Corporation


  1. Kraushaar DC, Jin W, Maunakea A, Abraham B, Ha M, Zhao K. Genome-wide incorporation dynamics reveal distinct categories of turnover for the histone variant H3.3. Genome Biol. 2013;14:R121.View ArticlePubMedPubMed CentralGoogle Scholar


© Kraushaar et al. 2016