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Erratum to: Dynamics of gene silencing during X inactivation using allele-specific RNA-seq

  • Hendrik Marks1Email author,
  • Hindrik H. D. Kerstens1,
  • Tahsin Stefan Barakat3,
  • Erik Splinter4,
  • René A. M. Dirks1,
  • Guido van Mierlo1,
  • Onkar Joshi1,
  • Shuang-Yin Wang1,
  • Tomas Babak5,
  • Cornelis A. Albers2,
  • Tüzer Kalkan6,
  • Austin Smith6,
  • Alice Jouneau7,
  • Wouter de Laat4,
  • Joost Gribnau3 and
  • Hendrik G. Stunnenberg1Email author
Genome Biology201617:22

Received: 25 January 2016

Accepted: 25 January 2016

Published: 5 February 2016

The original article was published in Genome Biology 2015 16:149

After the publication of this work [1], we noticed there was an error in Fig. 5 where −1,0 and 1 are incorrectly displayed in the y-axis in panel b. Please see the corrected Fig. 5 below. We apologize for this error.
Fig. 5

llele-specific RNA-seq on three NPC lines identifies three distal regions of genes that escape XCI. a Ratio of Xi/Xa (y-axis; for each of the three NPC lines sorted from highest to lowest) for genes showing a log2 ratio of at least −5. We set the cutoff for escape on 10 % relative expression from the Xi versus the Xa (log 2 ratio of > −3.32; similar to Yang et al. [37]). b Xi/Xa ratio of genes that escape XCI in all three NPC lines. c Distribution of the escape genes identified in *NPC_129-Xi over the four clusters as characterized in Fig. 4a. d Localization of the escape genes within each NPC line over the linear X chromosome (see also Table 1). The black dots on the fourth row represent all X-linked genes for which high-confidence allele-specific ratios were obtained in NPCs. e Validation of the escape genes within the three escape regions by Sanger sequencing of cDNA. See Additional file 1: Figure S13 for the full panel of 13 genes that we validated, and for further details



Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.

Authors’ Affiliations

Radboud University, Faculty of Science, Department of Molecular Biology, Radboud Institute for Molecular Life Sciences (RIMLS)
Radboud University, Faculty of Science, Department of Molecular Developmental Biology, Radboud Institute for Molecular Life Sciences (RIMLS)
Department of Reproduction and Development, Erasmus MC, University Medical Center
Hubrecht Institute, University Medical Center Utrecht
Biology Department, Queen’s University
Wellcome Trust-Medical Research Council Stem Cell Institute, University of Cambridge
INRA, UMR1198 Biologie du Développement et Reproduction


  1. Marks H, Kerstens HH, Barakat TS, Splinter E, Dirks RAM, van Mierlo G, et al. Dynamics of gene silencing during X inactivation using allele-specific RNA-seq. Genome Biol. 2015;16:149.View ArticlePubMedPubMed CentralGoogle Scholar


© Marks et al. 2016