Open Access

Erratum to: Visualizing translocation dynamics and nascent transcript errors in paused RNA polymerases in vivo

  • Masahiko Imashimizu1,
  • Hiroki Takahashi2,
  • Taku Oshima3,
  • Carl McIntosh1,
  • Mikhail Bubunenko1,
  • Donald L. Court1 and
  • Mikhail Kashlev1Email author
Genome Biology201516:270

https://doi.org/10.1186/s13059-015-0845-4

Received: 25 November 2015

Accepted: 25 November 2015

Published: 2 December 2015

The original article was published in Genome Biology 2015 16:98
After the publication of this work [1], we noticed an error whereby Fig. 3b is missing the label ΔgreAB. The original version of this article was corrected. The corrected figure is given below:
Fig. 3

Transcription pausing detected by RNET-seq in E. coli WT and ΔgreAB cells. (a) Pause-inducing elements (PIEs) of the non-template DNA strand. Information content is represented by sequence logos [51]. Positions −1 and −10 of DNA (gray) correspond to the RNA (blue) 3′ and 5′ ends of the RNA-DNA hybrid within RNAP (pink oval) in pre-translocated ECs. The active site is shown as an empty square. P(0.9, 100) and P(0.9, 160) were used for WT (n = 758) and ΔgreAB (n =419), respectively (see main text for the parameters). A frequency matrix and MAP scores for the PIEs are shown in Table S1 in Additional file 2. (b) Categorization of all RNAP pauses by RNA type. The non-coding RNA (ncRNA) and antisense RNA were defined using the gene annotation file of E. coli (see “Materials and methods”). (c) Pausing frequently occurs in regions proximal to transcript start sites (TSS). For panels B and C, P(0.7, 100) is used in order to increase the number of samples. Note that even when using this reduced stringency the consensus sequence for pausing remains unaffected (Fig. S6B in Additional file 1)

Notes

Declarations

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Authors’ Affiliations

(1)
Center for Cancer Research, National Cancer Institute
(2)
Medical Mycology Research Center, Chiba University
(3)
Graduate School of Biological Sciences, Nara Institute of Science and Technology

Reference

  1. Imashimizu M, Takahashi H, Oshima T, McIntosh C, Bubunenko M, Court DL, et al. Visualizing translocation dynamics and nascent transcript errors in paused RNA polymerases in vivo. Genome Biol. 2015;16:98.PubMed CentralView ArticlePubMedGoogle Scholar

Copyright

© Imashimizu et al. 2015

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