Fig. 1
From: Resources for the design of CRISPR gene editing experiments
Components of the CRISPR-Cas9 system. Streptococcus pyogenes Cas9 (SpCas9) forms a complex with a chimeric single guide RNA (sgRNA) comprising a spacer that hybridizes with the genomic target site, and a scaffold RNA termed tracrRNA required for complex formation. The protospacer adjacent motif (PAM) is required for sequence specificity of SpCas9-mediated endonuclease activity against genomic DNA