Figure 6

Periodic transcription is partially restored in the absence of Rad53 activity during the DNA replication checkpoint. Heat maps showing mRNA levels of a set of 343 WT periodic genes in cell-cycle arrested cdc8^ts;P _GALL -CDC20;cdc20;rad53-1 cells (DNA replication checkpoint without Rad53 kinase activity) (a), arrested cdc8^ts;cdc20;P _GALL -CDC20 cells (DNA replication checkpoint) (b), and cycling WT cells (c). The same order of genes is shown in all conditions (Additional file 3). Transcript levels are depicted as log₂-fold change relative to the mean expression and are depicted as in Figure 2. Transcript dynamics are shown for individual genes: YOX1 (d), FKH1 (e), NDD1 (f), and ACE2 (g). Log₂-fold mRNA levels for periodic genes in cdc8^ts;P _GALL -CDC20;cdc20;rad53-1 (no Rad53 activity; red), cdc8^ts;P _GALL -CDC20;cdc20 (DNA replication checkpoint; green), and WT (blue) cells (d-g).