Open Access

Networks in the yeast genome

  • Jonathan B Weitzman
Genome Biology20023:spotlight-20021029-01

https://doi.org/10.1186/gb-spotlight-20021029-01

Published: 29 October 2002

We are far from understanding the how complex transcriptional programs are generated from genomic information. In the October 25 Science, Lee et al. report how they constructed a map of transcriptional regulatory networks in Saccharomyces cerevisiae by identifying all the sites bound by a arge number of transcriptional regulators (Science 2002, 298:799-804). They performed genome-wide location analysis to find binding sites for all 141 transcription factors listed in the Yeast Proteome Database. They then introduced a myc epitope tag into the genomic sequences encoding each factor. Using immunoprecipitation and microarray analysis, almost 4,000 regulator-DNA interactions could be defined. More than a third of all yeast genes were bound by one or more of the regulators and many were bound by multiple factors. The average number of gene promoters bound by an individual factor was 38. These data were used to construct regulatory network that are made up of different motifs - autoregulation, multicomponent loops, feedforward loops, and so on. Lee et al. studied cell-cycle-regulated genes to illustrate how an automated approach combining genome-wide location and expression data can be used to generate complex regulatory network models.

References

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  4. A Transcriptional Regulatory Network Map for Saccharomyces cerevisiae., [http://web.wi.mit.edu/young/regulator_network]

Copyright

© BioMed Central Ltd 2002

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