Skip to main content

Regulating large chromatin domains

The thymocyte-specific SATB1 (special AT-rich sequence binding 1) protein binds to base-unpairing regions (BURs) of chromosomal DNA within matrix-attachment regions (MARs) and assembles a SATB1 network structure that can regulate gene expression over relatively large distances. In the October 10 Nature, Yasui et al. describe biochemical analysis of SATB1 within BUR-binding complexes (Nature 2002, 419:641-645). They analysed extracts from the thymi of normal and knockout (SATB1-/-) mice and found that components of the NURD, CHRAC and ACF chromatin-remodelling complexes co-purified with SATB1. Immunoprecipitation analysis showed that SATB1 recruits histone deacetylases and remodelling complexes, and represses the IL-2Ralpha (inteleukin-2 receptor alpha gene) locus. Changes in nucleosome positioning in the absence of SATB1 could be observed as much as 8 kilobases away, suggesting that mechanisms of this sort play a general a role in global gene regulation.

References

  1. 1.

    A tissue-specific MAR/SAR DNA-binding protein with unusual binding site recognition.

  2. 2.

    Nature, [http://www.nature.com]

Download references

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Weitzman, J.B. Regulating large chromatin domains. Genome Biol 3, spotlight-20021015-01 (2002). https://doi.org/10.1186/gb-spotlight-20021015-01

Download citation

Keywords

  • Network Structure
  • Biochemical Analysis
  • Regulate Gene Expression
  • Histone Deacetylases
  • Receptor Alpha