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Ribozyme targeting

Genome Biology20023:spotlight-20020321-01

  • Published:


  • Melanoma
  • Melanoma Cell
  • Cationic Liposome
  • Complex Phenotype
  • Metastatic Tumour

In the March 19 Proceedings of the National Academy of Sciences, Kashani-Sabet et al. describe the use of plasmid-based ribozymes as functional genomics tools to unravel complex phenotypes, such as cancer metastasis (Proc Natl Acad Sci USA 2002, 99:3878-3883). They reasoned that ribozyme-based gene targeting in mice might overcome experimental problems associated with transgenesis and lethal knockout phenotypes. They tested the use of systemic administration of cationic liposome:DNA complexes (CLDCs) to express hammerhead ribozymes in tumour-bearing mice; they targeted the p65 and p50 subunits of the NF-kappaB transcription factor using expression plasmids based on Epstein-Barr virus and including 35-bp ribozymes. They injected the CLDCs into the bloodstream of mice and noted a reduction in NF-κB proteins in metastatic tumour cells. The ribozymes affected the metastatic spread of melanoma cells; and the ribozymes appear to be more effective than antisense strategies, offering an experimental strategy to dissect complex traits in adult animals.


  1. Proceedings of the National Academy of Sciences, []
  2. Activators and target genes of Rel/NF-kB transcription factors.Google Scholar
  3. The experimental use of antisense oligonucleotides: a guide for the perplexed.Google Scholar


© BioMed Central Ltd 2002