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Transgenic frogs

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The binary Gal4-UAS system has been used to drive the tissue-specific expression of transgenes in a number of animal models. In the February 5 Proceedings of the National Academy of Sciences, Katharine Hartley and colleagues at the Wellcome/CRC Institute Cambridge, UK, report application of the Gal4-UAS system to create transgenic Xenopus (Proc Natl Acad Sci USA 2002, 99:1377-1382). They generated Xenopus lines expressing constructs for the 'activator', the yeast transcription factor Gal4, or 'effector', tandem repeats of the yeast Gal4-binding motif (UAS). In this way they could drive tissue-specific, or stage-specific, transgene expression. Hartley et al. used the Gal4-UAS system to regulate expression of the ventralizing homeobox gene Xvent-2, resulting in severe developmental and microcephalic phenotypes. They propose that such misexpression studies will be useful for extensive gain-of-function experiments in Xenopus.

References

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    GAL4 activates transcription in Drosophila.

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    Proceedings of the National Academy of Sciences, [http://www.pnas.org]

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    Wellcome/CRC Institute , [http://www.welc.cam.ac.uk]

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Weitzman, J.B. Transgenic frogs. Genome Biol 3, spotlight-20020130-01 (2002) doi:10.1186/gb-spotlight-20020130-01

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Keywords

  • Transcription Factor
  • Animal Model
  • Tandem Repeat
  • Homeobox Gene
  • Report Application