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Antisense RNA

Gene silencing by RNA interference (RNAi) has become a powerful tool for functional genomics. In the January 25 Science, Tijsterman et al. report a gene-silencing method induced by short (25 nucleotide) antisense RNA molecules (Science 2002, 295:694-697). They found that injecting single-stranded, antisense RNA (asRNA) oligomers into the gonadal syncytium of Caenorhabditis elegans resulted in silencing of the maternal pos-1 gene and of germline-expressed genes. Tijsterman et al. found that asRNA silencing requires the mutator/RNAi genes mut-7 and mut-14. They cloned the mut-14 gene and report that it encodes a putative RNA helicase with a DEAD-box motif. The authors propose that asRNA-induced gene-silencing may result from the priming of RNA synthesis on mRNA templates, thereby creating dsRNA for subsequent degradation.


  1. Functional anatomy of a dsRNA trigger: differential requirement for the two trigger strands in RNA interference.

  2. Science, []

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Weitzman, J.B. Antisense RNA. Genome Biol 3, spotlight-20020125-01 (2002).

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