Skip to content


  • Research news
  • Open Access

Ultraviolet sensitivity

Genome Biology20012:spotlight-20011022-01

  • Published:


  • Powerful Approach
  • Deletion Strain
  • Oligonucleotide Array
  • Deletion Analysis
  • Radiation Sensitivity

The systematic deletion of all yeast ORFs, in the SaccharomycesGenome Deletion Project, provides a powerful resource for large-scale 'parallel deletion analysis'. In the October 23 Proceedings of the National Academy of Sciences, Geoff Birrell and colleagues at Stanford University School of Medicine describe a screen for sensitivity to a genome-damaging agent (Proc Natl Acad Sci USA 2001, 98:12608-12613). They screened pools of 4,627 deletion strains for killing by ultraviolet (UV) irradiation and used oligonucleotide arrays to follow radiation sensitivity. They identified 25 known UV-sensitive deletions and found three new genes not previously linked with UV sensitivity. Two of these, CaSm and AF9, have human orthologs implicated in cancer, demonstrating how such a powerful approach can provide important insights into the mechanism of cytotoxicity by genotoxic agents.


  1. Saccharomyces Genome Deletion Project, []
  2. Quantitative phenotypic analysis of yeast deletion mutants using a highly parallel molecular bar-coding strategy.Google Scholar
  3. Proceedings of the National Academy of Sciences, []
  4. Stanford University School of Medicine , []
  5. The Saccharomyces repair genes at the end of the century.Google Scholar


© BioMed Central Ltd 2001