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Genome Biology volume 2, Article number: spotlight-20011022-01 (2001)
The systematic deletion of all yeast ORFs, in the SaccharomycesGenome Deletion Project, provides a powerful resource for large-scale 'parallel deletion analysis'. In the October 23 Proceedings of the National Academy of Sciences, Geoff Birrell and colleagues at Stanford University School of Medicine describe a screen for sensitivity to a genome-damaging agent (Proc Natl Acad Sci USA 2001, 98:12608-12613). They screened pools of 4,627 deletion strains for killing by ultraviolet (UV) irradiation and used oligonucleotide arrays to follow radiation sensitivity. They identified 25 known UV-sensitive deletions and found three new genes not previously linked with UV sensitivity. Two of these, CaSm and AF9, have human orthologs implicated in cancer, demonstrating how such a powerful approach can provide important insights into the mechanism of cytotoxicity by genotoxic agents.
Saccharomyces Genome Deletion Project, [http://www-sequence.stanford.edu/group/yeast_deletion_project/deletions3.html]
Quantitative phenotypic analysis of yeast deletion mutants using a highly parallel molecular bar-coding strategy.
Proceedings of the National Academy of Sciences, [http://www.pnas.org]
Stanford University School of Medicine , [http://www-med.stanford.edu]
The Saccharomyces repair genes at the end of the century.
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Weitzman, J.B. Ultraviolet sensitivity. Genome Biol 2, spotlight-20011022-01 (2001) doi:10.1186/gb-spotlight-20011022-01
- Powerful Approach
- Deletion Strain
- Oligonucleotide Array
- Deletion Analysis
- Radiation Sensitivity