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Open Access

Topo II & pol II

  • Jonathan B Weitzman
Genome Biology20012:spotlight-20011003-01

https://doi.org/10.1186/gb-spotlight-20011003-01

Published: 03 October 2001

The core RNA polymerase II (pol II) requires components of the holoenzyme (holo-pol) complex to drive transcription from chromatin templates. In the September 27 Nature, Neelima Mondal and Jeffrey Parvin from the Brigham and Women's Hospital, Boston, USA, report that DNA topoisomerase II alpha (topo II) is required for chromatin-dependent coactivation (Nature 2001, 413:435-438). They demonstrated that topo II is a component of the holo-pol complex and confered the ability to relax supercoiled DNA. Topo II inhibitors, such as etoposide, prevented pol II transcription on chromatin templates. The authors suggest that an additional factor is important for loading topo II onto pre-formed chromatin templates. They propose a model in which topo II is required to relieve superhelical tension generated during transcription by pol II.

References

  1. An RNA polymerase II holoenzyme responsive to activators.Google Scholar
  2. Nature , [http://www.nature.com]
  3. Brigham and Women's Hospital , [http://www.brighamandwomens.org/]
  4. Functional and regulatory characteristics of eukaryotic type II DNA topoisomerase.Google Scholar

Copyright

© BioMed Central Ltd 2001

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