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Profiling and policing
Genome Biology volume 2, Article number: spotlight-20010625-01 (2001)
Cell lines used in many laboratories are often not what they are claimed to be, resulting in misleading research articles. A well-characterized case of cross-contamination is the widely used human cancer cell line called HeLa. In the Early Edition of the Proceedings of the National Academy of Sciences, Masters et al. describe the use of forensic techniques to expose culprit samples. Short tandem repeat (STR) profiling is a simple PCR-based technique that generates results in the form of a standard numerical code for lengths of polymorphic loci. Masters et al. analysed 253 human cell lines, collected from international cell banks and cancer research institutes, to demonstrate the feasibility of wide-scale STR profiling to detect cross-contamination. The authors suggest that STR profiling (at a cost of only $200 per cell line) could be used to create an international reference standard for human cell lines and they propose that a policy of 'authentication prior to publication' would diminish scientific misrepresentation.
Cell contamination leads to inaccurate data: we must take action now
Widespread intraspecies cross-contamination of human tumor cell lines arising at source.
Proceedings of the National Academy of Sciences , [http://www.pnas.org]
Short tandem repeat profiling provides an international reference standard for human cell lines, [http://www.pnas.org/cgi/doi/10.1073/pnas.121616198]
A highly discriminating octoplex short tandem repeat polymerase chain reaction system suitable for human individual identification.