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Automatable SNP assay

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In the February 27 Proceedings of the National Academy of Sciences, Bartlett et al. describe a simple technique for single-nucleotide polymorphism (SNP) analysis that could be easily automated for high-throughput SNP typing (Proc Natl Acad Sci USA 2001, 98:2694-2697). The fluorescence technique (which the authors dub ADMI, alkaline-mediated differential interaction) is adapted from the amplification-refractory mutation system (ARMS)-PCR and exploits the double-stranded DNA-specific dye SYBR green I, combined with detection at buffered high pH conditions. The technique is inexpensive, automatable, simple and robust, making it ideal for high-throughput applications. Bartlett et al. tested the performance of the assay using 32 sequence-specific primer mixes to identify HLA genotypes of 80 independent lymphoblastoid cell lines.

References

  1. 1.

    Proceedings of the National Academy of Sciences, [http://www.pnas.org]

  2. 2.

    A Database of Single Nucleotide Polymorphisms, [http://www.ncbi.nlm.nih.gov/SNP/index.html]

  3. 3.

    Tissue typing the HLA-A locus from genomic DNA by sequence-specific PCR: comparison of HLA genotype and surface expression on colorectal tumor cell lines.

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Weitzman, J.B. Automatable SNP assay. Genome Biol 2, spotlight-20010227-01 (2001) doi:10.1186/gb-spotlight-20010227-01

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Keywords

  • Simple Technique
  • Lymphoblastoid Cell Line
  • Fluorescence Technique
  • SYBR Green
  • Mutation System