Skip to main content
  • Research news
  • Published:

Automatable SNP assay

In the February 27 Proceedings of the National Academy of Sciences, Bartlett et al. describe a simple technique for single-nucleotide polymorphism (SNP) analysis that could be easily automated for high-throughput SNP typing (Proc Natl Acad Sci USA 2001, 98:2694-2697). The fluorescence technique (which the authors dub ADMI, alkaline-mediated differential interaction) is adapted from the amplification-refractory mutation system (ARMS)-PCR and exploits the double-stranded DNA-specific dye SYBR green I, combined with detection at buffered high pH conditions. The technique is inexpensive, automatable, simple and robust, making it ideal for high-throughput applications. Bartlett et al. tested the performance of the assay using 32 sequence-specific primer mixes to identify HLA genotypes of 80 independent lymphoblastoid cell lines.

References

  1. Proceedings of the National Academy of Sciences, [http://www.pnas.org]

  2. A Database of Single Nucleotide Polymorphisms, [http://www.ncbi.nlm.nih.gov/SNP/index.html]

  3. Tissue typing the HLA-A locus from genomic DNA by sequence-specific PCR: comparison of HLA genotype and surface expression on colorectal tumor cell lines.

Download references

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Weitzman, J.B. Automatable SNP assay. Genome Biol 2, spotlight-20010227-01 (2001). https://doi.org/10.1186/gb-spotlight-20010227-01

Download citation

  • Published:

  • DOI: https://doi.org/10.1186/gb-spotlight-20010227-01

Keywords