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© BioMed Central Ltd 2000
- Published: 23 November 2000
- Genome Sequence
- Pole Body
- Spindle Pole
- Hybrid Assay
- Yeast Genome
Two-hybrid assaysare great for detecting protein-protein interactions, but they scale as the square of the number of candidate interactions. Screening every possible interaction in yeast would require examining approximately 3.6 x 107 pairs, which is why previous large-scale screens byUetz et al. and Ito et al. have pooled libraries of constructs. In the November 21 Proceedings of the National Academy of Sciences, Newman et al. find that such approaches can miss a great number of valid interactions (Proc Natl Acad Sci USA 2000, 97:13203-13208). Newman et al. approach the problem by first screening the yeast genome sequence computationally for the coiled-coil interaction motif, which consists of two or more helices that wrap around each other. They then analyze 162 coiled-coil sequences, selected on the basis of either their high predicted coil scores or their association with mitotic processes, and use two hybrid assays to find 213 unique interactions. The results reveal an extraordinary web of interactions at the yeast spindle pole body, and an unexpected connection between spindle pole and kinetochore proteins.
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