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An inchworm unwinds
Genome Biology volume 1, Article number: spotlight-20000526-02 (2000)
In the 18 May Nature, Blanco and Kowalczykowski report on the motions of the RecBC DNA helicase, a protein that unwinds DNA strands during homologous recombination in Escherichia coli. The helicase needs a double-stranded blunt end to load onto DNA, but can then move along a single strand from 3' to 5'. A large gap in this strand causes the helicase to fall off. If the gap is shorter, however, the helicase leaps over the gap (Nature 2000, 405:368-372). By varying the length of the initial double-stranded section and the subsequent single-stranded gap, the researchers show that the helicase moves in approximately 23-nucleotide steps from its point of loading. A helicase that initially traverses 31 nucleotides of double-stranded DNA, for example, can subsequently jump a gap up to a maximum of 15 nucleotides in length. Blanco and Kowalczykowski propose that the helicase domain catches up to the leading binding domain in multiple steps of 2-5 basepairs each.
Helicases: a unifying structural theme?
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Wells, W. An inchworm unwinds. Genome Biol 1, spotlight-20000526-02 (2000). https://doi.org/10.1186/gb-spotlight-20000526-02
- Escherichia Coli
- Binding Domain
- Homologous Recombination
- Single Strand