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Transfecting with antibodies
Genome Biology20001:spotlight-20000523-02
https://doi.org/10.1186/gb-spotlight-20000523-02
© BioMed Central Ltd 2000
- Published: 23 May 2000
Keywords
- Cell Membrane
- Cell Surface
- Target Cell
- Gene Therapy
- Cell Killing
The various available transfection methods suffer from low efficiency, lack of specificity for a particular cell type, and a tendency to kill many of the target cells. Bildirici et al. offer a solution in the 18 May Nature, using antibody-coated beads (Nature 2000, 405:298). DNA enters the cells after agitation of a bead-cell mixture tears holes in the cell membrane. Transfection efficiency ranges from 40 to 80% with less than 20% cell killing, and the DNA is targeted to cells with the relevant cell surface marker. This targeting will be particularly important for ex vivo gene therapy applications.
References
- Nature magazine, [http://www.nature.com/nature/]
Copyright
© BioMed Central Ltd 2000