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Plant interference

A simple method for generating loss-of-function plant mutants is described by Chuang and Meyerowitz in the April 25 Proceedings of the National Academy of Sciences (Proc. Natl. Acad. Sci. USA 2000, 97:4985-4990 [Read the abstract on PubMed]). Chuang and Meyerowitz use Agrobacterium-mediated transformation of Arabidopsis thaliana to achieve RNA interference. Their sense/antisense construct produces a hairpin double-stranded RNA. This reproduces the loss of gene function that has been seen in other organismsafter injection of double-stranded RNA. There is, however, some variability in the strength of the response depending on the identity of the target gene.

References

  1. 1.

    The Meyerowitz Lab homepage, [http://broccoli.caltech.edu/~meyerowitz/html/.index.html]

  2. 2.

    PNAS online journal, [http://www.pnas.org/]

  3. 3.

    Specific and heritable genetic interference by double-stranded RNA in arabidopsis thaliana.

  4. 4.

    Genetic requirements for inheritance of RNAi in C. elegans.

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Wells, W. Plant interference. Genome Biol 1, spotlight-20000428-03 (2000). https://doi.org/10.1186/gb-spotlight-20000428-03

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Keywords

  • Target Gene
  • Gene Function
  • Arabidopsis Thaliana
  • Plant Mutant
  • Plant Interference