Genome sequencing of the high oil crop sesame provides insight into oil biosynthesis
Genome Biology volume 15, Article number: R39 (2014)
Sesame, Sesamum indicum L., is considered the queen of oilseeds for its high oil content and quality, and is grown widely in tropical and subtropical areas as an important source of oil and protein. However, the molecular biology of sesame is largely unexplored.
Here, we report a high-quality genome sequence of sesame assembled de novo with a contig N50 of 52.2 kb and a scaffold N50 of 2.1 Mb, containing an estimated 27,148 genes. The results reveal novel, independent whole genome duplication and the absence of the Toll/interleukin-1 receptor domain in resistance genes. Candidate genes and oil biosynthetic pathways contributing to high oil content were discovered by comparative genomic and transcriptomic analyses. These revealed the expansion of type 1 lipid transfer genes by tandem duplication, the contraction of lipid degradation genes, and the differential expression of essential genes in the triacylglycerol biosynthesis pathway, particularly in the early stage of seed development. Resequencing data in 29 sesame accessions from 12 countries suggested that the high genetic diversity of lipid-related genes might be associated with the wide variation in oil content. Additionally, the results shed light on the pivotal stage of seed development, oil accumulation and potential key genes for sesamin production, an important pharmacological constituent of sesame.
As an important species from the order Lamiales and a high oil crop, the sesame genome will facilitate future research on the evolution of eudicots, as well as the study of lipid biosynthesis and potential genetic improvement of sesame.
Sesame (Sesamum indicum), a widely grown crop in tropical and subtropical areas, is documented as the most ancient oil crop providing humans with essential daily energy. Vegetable oil consumption is expected to reach almost 200 billion kilograms by 2030 , which will increase the demand for oil-rich crops; genetic studies to improve oil content in vegetables will help address this demand. Compared to other edible oil crops such as soybean (Glycine max), rapeseed (Brassica napus), peanut (Arachis hypogaea) and olive (Olea europaea), sesame has innately higher oil content (approximately 55% of dry seed) , and is thus an attractive potential model for studying lipid biosynthesis .
The sesame seed has been considered the ‘queen of oilseeds’ for its high oil content and quality , and has been traditionally categorized as a health food in China, Japan and other East Asian countries . The antioxidative furofuran lignans in sesame have been analyzed by pharmacologists for their potent pharmacological properties in decreasing blood lipids  and lowering cholesterol levels . Recently, the gene encoding sesamin synthase was identified .
Taxonomically, sesame belongs to Lamiales, an order comprising of 23,810 flowering plants in the clade asterids . Lamiales includes many other well-known or economically important species, such as olive (O. europaea), leonurus (Leonurus japonicas), lavender (Lavandula spica) and basil (Ocimum basilicum). However, few Lamiales species have been the subject of intensive genetic or genomics studies. As high-throughput sequencing has become routine, several studies in sesame have been performed. A number of simple sequence repeat (SSR) markers have been developed [10–12]. The loci associated with indehiscent capsule trait , determinate growth habit  and seed coat color  have been detected, and the expression levels of sesame genes have been explored using Sanger and high-throughput DNA sequencing technologies [16–18]. The phylogenetic position of sesame has been determined using chloroplast genomic data, which indicated the core lineage of Sesamum in Lamiales . These studies together with the recently published minute genome of Utricularia gibba and a genome survey of sesame  have contributed new insight into the Lamiales.
Here, we report a high-quality draft genome of the sesame genotype ‘Zhongzhi No. 13’ , an elite cultivar with high oil content (59%), which has been introduced to most major sesame planting areas in China over the past 10 years. In addition to the well-assembled genome sequences, a new high-density genetic map, 12 in-depth RNA-Seq data sets and resequencing data for 29 sesame accessions were generated to help understand and analyze genome structure, evolution and important nutritional characters, including lipid and sesamin synthesis, in the most comprehensive way. Together, these results will open a door for genetic studies for a variety of purposes in, but not restricted to, sesame.
Results and discussion
De novogenome sequencing
Assembly and assessment
After reads filtering, 54.5 Gb of high-quality data from sesame cultivar ‘Zhongzhi No. 13’ were obtained using the Illumina Hiseq2000 platform (Figure S1 and Tables S1 and S2 in Additional file 1; Data S1 in Additional file 2), representing approximately 152.7-fold coverage of the predicted sesame genome. SOAPdenovo  was used to assemble the genome, which resulted in a draft genome of 274 Mb with contig N50 of 52.2 kb and scaffold N50 of 2.1 Mb, which are approximately 2.7- and 92.9-fold longer, respectively, than a previous survey of the sesame genome  (Table 1; Tables S3 and S4 in Additional file 1). Using a newly constructed genetic map consisting of 406 markers (Data S2 in Additional file 2), we anchored 150 large scaffolds (117 oriented) into 16 pseudomolecules, which harbored 85.3% of the genome assembly and 91.7% of the predicted genes (numbered LG1 to LG16; Table 1; Figure 1; Table S5 and Figures S3 to S5 in Additional file 1). The estimated heterozygosity of the assembled sequenced line was 1.08 × 10−4. This low heterozygosity was not unexpected because sesame is a self-pollinated plant , and we had performed successive selfing for five generations on the sample before sequencing to guarantee its homozygosity.
The assembly covered 77.4 to 81.3% of the genome size according to the estimations derived from 17-mer depth distribution (357 Mb) (Figure S2 in Additional file 1) and flow cytometry (337 Mb) (Figure S3 in Additional file 1). The integrity of gene space in the genome assembly was demonstrated by the successful mapping of 99.3% of 3,328 expressed sequence tags (ESTs)  retrieved from GenBank, and 98.5% of 86,222 unigenes that were assembled de novo from previously reported RNA-Seq data  (Table S6 in Additional file 1). In addition, the large-scale assembly accuracy was assessed using five fosmid clones (33.5 to 38.6 kb) that were sequenced thoroughly using the Sanger sequencing technology (see Supplementary Note in Additional file 1), whereby 99.6% of the clone sequences, on average, were identical to the assembly (Table S7 and Figure S7 in Additional file 1). Hence, a high-quality assembly of sesame is provided here, rendering it a valuable source for studying genome structure and evolution.
We predicted 27,148 protein-coding genes with an average transcript size of 3,171 bp by ab initio and homology-based analyses (Tables S8 to S10 in Additional file 1), together with RNA-Seq reads-assisted annotation. Of those, 23,635 (87.1%) were supported by unigenes or protein similarity, with only 12.9% derived solely from ab initio gene predictions (Table S9 in Additional file 1). With regard to non-coding genes, we identified 207 microRNAs, 870 tRNAs, 268 small nuclear RNAs, and 386 rRNA fragments from the assembly (Table S11 in Additional file 1).
Transposable elements play an important role in and are the major components of plants. A comprehensive annotation revealed that the repeat elements in sesame are lower (28.5% of assembly) than in grapevine (52.2%) , tomato (63.2%)  and potato (54.5%)  (Table 1; Tables S12 and S13 in Additional file 1). As observed in other sequenced genomes, long terminal repeats (LTRs) in sesame occupy the majority (51.1%) of repeat sequences. When the number of full-length LTRs (Copia and Gypsy) was plotted against their insertion time inferred from the intra-sequence divergence of LTR regions (Figure S8 in Additional file 1), the resulting age distributions exhibited typical ‘L’ shapes with the accumulation of many recent LTRs and much fewer old LTRs. These age distributions reflected a steady-state stochastic birth/death model for the dynamics of LTR accumulation and activity .
For the two major members of LTRs, the proportion of Copia (7.3% of the genome) in sesame is comparable to that in grapevine, tomato and potato, but the percentage of Gypsy (6.6%) is extremely underrepresented (Table S13 in Additional file 1). Unlike that observed in tomato and potato, the distribution of the divergence rate of Gypsy is very smooth and low in sesame, suggesting that it had not experienced explosive accumulation or activity (Figure S9 in Additional file 1), and may be associated with the low proportion of Gypsy, which in turn relates to the lower repeat element ratio and smaller genome size of sesame.
Evolution in the sesame genome
Gamma and a recent whole genome duplication event in sesame
Sesame belongs to the asterids clade of eudicots . Taxonomically, it is mostly related to Utricularia gibba, tomato  and potato , the other whole genome sequences available and published thus far in this clade. Based on the shared single-copy genes from 11 sequenced species (Table S14 and Figure S10 in Additional file 1), sesame was estimated to have diverged from U. gibba approximately 98 million years ago (68.6 to 145.2 MYA), and from the tomato-potato lineage approximately 125 MYA (89.8 to 185.8 MYA) (Figure S11 in Additional file 1).
We identified 11,934 shared dicot-monocot, 14,158 shared asterids-rosids (two clades of dicots) and 20,563 shared asterids lineage (sesame, U. gibba, tomato and potato) gene clusters (Figure 2a), representing their ancestral gene families. Moreover, we identified 450 gene families containing 2,638 genes, plus 3,972 single-copy genes that were specific to sesame (Table S14 and Figure S10 in Additional file 1; Data S3 and S4 in Additional file 2). Many of these genes were or encoded P450 genes, zinc finger proteins, transposases, transcription factors and disease resistance genes, suggesting their possible roles in species differentiation and adaptability in the sesame lineage or Lamiales.
Based on synonymous substitution rates (Ks) and synteny relationships, we uncovered the dicot-specific gamma (γ) event and a novel independent whole genome duplication (WGD) event in the lineage leading to sesame (Figures S12 and S13 in Additional file 1). The recent WGD event in the sesame lineage was further corroborated in that the single grapevine region always aligned with two sesame segments (Figure 2b; Table S15 and Figure S14 in Additional file 1). We tentatively partitioned the recent sesame lineage-specific WGD genomic regions into two non-overlapping ‘subgenomes’. The two subgenomes of the WGD corresponded to approximately 61 Mb (7,781 genes) and approximately 74 Mb (7,975 genes) regions, respectively, constituting approximately 50% of the current sesame genome assembly. Among all referred grape genomic loci, 79.1% were found to have undergone substantial gene loss, with a copy retained in only one of duplicated syntenic regions (subgenomes) of the sesame genome (Supplementary Note and Tables S16 and S17 in Additional file 1; Data S5 and S6 in Additional file 2), following the WGD that occurred in the sesame lineage. To estimate the age of recent WGD events in the sesame genome, we extracted 1,239 duplicated sesame genes (both retained in two subgenomes; Data S7 in Additional file 2) and calculated their Ks values. We observed nearly parallel peaks and identical ranges in the Ks distributions of these duplicated gene pairs and those from the triplication event in tomato (Figure S15 in Additional file 1), which have been dated to approximately 71 (±19) MYA . Therefore, the recent WGD event of sesame should have occurred independently in the parallel period of the triplication event in the tomato-potato lineage, but be older than the most recent duplication in U. gibba after their divergence (Figure 2c). Genes retained in duplicate are not evenly distributed among different functional categories , and an obvious bias was observed for the genes corresponding to transport, regulation, signal transduction and metabolism in sesame (Table S18 in Additional file 1). These over-retained genes may function necessarily in increasing the complexity of the regulatory networks accountable for the interaction between genotype and environment for the species after WGD.
Absence of amino-terminal Toll⁄interleukin-1 receptor nucleotide binding sites encoding resistance genes in sesame
Genes encoding nucleotide-binding sites (NBSs) are the largest class of plant disease resistance genes. Based on whether they contain a Toll⁄interleukin-1 receptor (TIR) domain , NBS resistance genes can be further categorized into two subclasses (TIR and non-TIR). We identified a total of 171 genes with an NBS domain in sesame (Table S19 in Additional file 1), and 65.2% were organized in tandem arrays (Figure S16 in Additional file 1). Intriguingly, all of the NBS-encoding resistance genes were not the TIR type. The absence of TIR domain-containing resistance genes has been reported generally in monocots , but is rare in eudicots, although it has been detected in sugar beet (Beta vulgaris) by PCR . Analysis of homologous genes from 11 species, including 8 eudicots and 3 monocots, has also shown that sesame and monocots are absent from the OrthoMCL clusters of genes with the TIR domain (Data S8 in Additional file 2; Figure S17 in Additional file 1). The absence of NBS genes with the TIR domain in sesame was further examined by searching the gene-masked assembly and the unmapped reads; neither showed a TIR domain in sesame. Hence, the unambiguous absence of TIR domain-containing resistance genes at the whole genome scale in sesame, a species of eudicots, provides a new paradigm for the study of the evolution of resistance genes . However, the mechanism that induced such loss and whether it is common in the order Lamiales require further elucidation.
Quality characters of sesame
Molecular foundation for the high oil content of sesame
Elucidation of the sesame genome allowed the unprecedented opportunity to study oil biosynthesis to understand its high oil content. By searching the lipid-related gene database consisting of 222 functional families of Arabidopsis thaliana, we found that sesame has unexpectedly low gene copy numbers (708) compared to A. thaliana (736), soybean (1,298), grapevine (732), tomato (902) and rice (805) (Data S9 in Additional file 2). For the two edible oil crops, the conspicuous discrepancy between sesame and soybean in their oil content (approximately 55% versus approximately 20%, respectively, of dry seed) and their predicted lipid gene number (708 versus 1,298, respectively) implies that different properties or mechanisms of oil biosynthesis exist in the two distantly related oil crops.
In contrast, soybean contains more copies than sesame of approximately 94.1% of the 222 lipid-related gene families. We found that the families encoding lipid transfer protein type 1 (LTP1), midchain alkane hydroxylase, FAD4-like desaturase (FAD4-like), and alcohol-forming fatty acyl-CoA reductase (AlcFAR) have been expanded by tandem duplication in sesame (Figures S18 to S20 in Additional file 1). Among these families, LTP1 is the largest, containing 34 genes with 29 clustering into 4 tandem arrays (Figure 3a). The high sequence similarity among the genes in tandem 3 and 4 suggested that each might have experienced a recent expansion. Of these LTP1 genes, more than 90% were expressed in a set of 12 sesame seed transcriptomes with RPKM (Reads per kilobase of exon per million reads mapped) greater than 1), confirming their functional activities in lipid biosynthesis (Figure 3b). Expansion and retention of these genes may reflect the selection for genomic variation corresponding to the production of high oil content during domestication because the enhancement of the LTP1 family may benefit oil accumulation by strengthening the transport of fatty acids, acyl-CoAs, and other lipid molecules . In addition, the two cytosolic lipoxygenase (LOX) and lipid acyl hydrolase-like (LAH) families related to the degradation of lipids [36, 37] are both contracted in sesame (8 LOXs and 18 LAHs) when compared to soybean (45 LOXs and 42 LAHs) (Figures S21 and S22 in Additional file 1). Based on these data, we speculate that the expansion of some lipid gene families, especially the type 1 lipid transport genes, and the contraction of lipid degradation-related families may lead to higher oil content in sesame than soybean.
Differentially expressed lipid genes in seed development
To investigate the potential mechanism underlying the variation in oil content in sesame, we evaluated two accessions representing low oil content materials (48% and 51%) in germplasm, along with the high oil content cultivar ‘Zhongzhi No. 13’ (59%), each in four developmental stages (10, 20, 25, and 30 days post anthesis (DPA)) for RNA-Seq analysis (Table S1 in Additional file 1). Clustering of the expression profiles of the 416 sesame genes that were predicted to be orthologous to the lipid-related genes of A. thaliana (Supplementary Note in Additional file 1) clearly distinguished the 10 DPA from other stages and also the high and low oil sesame accessions (Figure 4a), suggesting that the determination of different oil content by lipid-related genes begins in the early stages of seed development.
After checking all oil-related pathways, we found that the genes expressed differentially at 10 DPA were enriched significantly in genes downstream of the triacylglycerol (TAG) biosynthesis pathway, including genes encoding phosphatidylcholine:diacylglycerol cholinephosphotransferase (PDCT) , phospholipid:diacylglycerol acyltransferase (PDAT) , oil-body proteins (steroleosin, caleosin and oleosin), and transcription factors (ABI3, ABI4 and WRI1) (Data S10 in Additional file 2). Until recently, the last step in TAG biosynthesis was assumed to be uniquely catalyzed by acylCoA:diacylglycerol acyltransferase (DGAT). However, some plants (for example, sunflower, castor bean and Crepis palaestina) and yeast were found to have an acyl-CoA-independent mechanism for TAG synthesis, which uses phospholipids as acyl donors and diacylglycerol as the acceptor [40, 41]. In the present study, PDAT was expressed 2- to 3.5-fold higher in the one high-oil accession than the two low-oil accessions at 10 DPA (Figure 4b), whereas DGAT showed no significant differences in expression. These results are in accordance with the results in yeast, which revealed that overexpression of PDAT can increase TAG by two-fold in the early logarithmic phase [41, 42]. Collectively, these data strongly suggested that the expression of PDAT in collaboration with other genes plays a pivotal role in shaping oil accumulation in the early stage of sesame seed development.
Population variation in sesame lipid-related genes
To screen the sequence variation in lipid-related genes, 29 sesame accessions from 12 countries with oil content variation ranging from 48.6% to 59.8% were selected for genome resequencing. More than 120 Gb clean data corresponding to 13-fold genomic coverage for each accession were generated, resulting in the identification of 2,348,008 SNPs (Data S11 in Additional file 2). From these SNPs, population diversity (π) and Watterson’s estimator of segregating sites (θw) were estimated to be 0.0025 and 0.0032, respectively, in the population (Figure 5a; Figure S23 in Additional file 1). This genetic diversity is lower than that in rice , but higher than that in chickpea (Cicer arietinum) , watermelon (Citrullus lanatus)  and soybean  (Table S20 in Additional file 1). Lipid-related genes in sesame showed a wide variation with π values ranging from 0 to 0.0099, and were similar to the average of other genes (0.0021 versus 0.0020). In addition, lipid-related genes with high π values (top 10%) were significantly (P < 0.0001) enriched in the two biological processes of lipid transport and lipid localization. For example, most genes in the LTP1 family exhibited high diversity (Figure 5b). Specifically, the ‘youngest’ , in tandem 4, nearly had all of the highest π values in lipid genes. Furthermore, using read depth of coverage, we found that about a quarter of the 708 predicted lipid-related genes in sesame had copy number variations (Data S12 in Additional file 2) that were enriched significantly (P < 0.01) in the biological process of lipid transport and the molecular function of lipid binding. For the LTP1 genes, three of tandem 1, three of tandem 2, four of tandem 3 and eight of tandem 4 were observed to exhibit copy number variation (maximum of two to four copies) among the population (Data S13 in Additional file 2). The abundant variation in LTP1 might be associated with the intra-species differences in oil content.
We also found that the oleate desaturase (FAD2, π = 0.0016) and linoleate desaturase (FAD8, π = 0.0018) genes usually have low diversity, which may partially explain the low variation (40 ± 5% of oil) in oleic and linoleic acid content in sesame accessions . For the oil-body protein families, higher variation was detected in the caleosin family (with an average π value of 0.0047) than in the oleosin (0.0025) and steroleosin (0.0024) families. In contrast, transcription regulatory families containing WRI1, ABI3 and ABI4 exhibited relatively low π values (<0.0008), indicating a conserved function for these transcription factors that participate in seed development and oil biosynthesis.
Genes for sesamin biosynthesis
Sesamin is an oil-soluble furofuran lignan typically present in sesame seed. It can increase oil stability and has been reported to be positively correlated with oil content . Sesamin biosynthesis involves two key genes encoding dirigent protein (DIR) and piperitol/sesamin synthase (PSS), respectively  (Figure S24a in Additional file 1). By conducting a BLAST search against the 11 species (Table S14 in Additional file 1), we found that the DIR homologues are present in sesame (SIN_1015471) and tomato, but the PSSs are only detected in sesame (SIN_1025734), indicating the genetic foundation for the sesame-specific product.
The gene expression level usually correlates with its product . In the four stages of three sesame accessions with different sesamin content (Table S1 in Additional file 1), DIR (SIN_1015471) expression was generally highest in the three 10 DPA seed samples, which decreased in the following stages. In contrast, PSS showed different patterns among the three accessions (Figure S24b in Additional file 1), with the highest expression levels detected at 20 DPA in the low sesamin accessions as reported previously  but at higher levels than detected at 10 DPA in the high sesamin accession. The higher PSS level in the early seed developmental stage in the high sesamin accession might be associated with its dual catalytic property, thereby producing more piperitol for subsequent sesamin biosynthesis. Although we expected to find PSS sequence variations between the high and low sesamin accessions, we found that it was especially conserved when we checked the 29 resequenced accessions (the three accessions used for RNA-Seq were also included). Thus, we speculated that other genes might regulate the different expressions of PSS; therefore, we selected a list of co-expressed genes of PSS for further study (Data S14 in Additional file 2). These genes are mainly involved in metabolic processes and catalytic activity (Figure S25 in Additional file 1).
The de novo assembled genome of sesame offers a new whole genome sequence in the order Lamiales that follows the typical minute genome U. gibba. This information constitutes an important resource for genetic and evolutionary studies. The evolutionary scenario outlined for sesame clearly reveals a more recent WGD event at approximately 71 ± 19 MYA, which occurred after the split from tomato and potato and presents a new resource for studying the intricate paleopolyploidization processes in plants. The evolution of genes in Lamiales or asterids may be more complicated, considering the complete loss of the TIR-type NBS-encoding resistance genes in sesame, which undoubtedly presents a new paradigm in elucidating the fate of resistance genes along with their interactions with diseases. Moreover, determining whether similar mechanisms exist that induce or offset the loss of the TIR domain in both eudicots and monocots will be of great interest.
Although many studies have focused on the mechanisms of lipid biosynthesis and accumulation and the detection of lipid-related genes in different species, the genes involved in the dozens of complex oil biosynthesis pathways require further elucidation. For primary edible oil crops such as rapeseed, peanut and soybean, it is inextricable to encounter intertwined polyploidy or large genome sizes with many lipid-relates genes, which makes studies on oil biosynthesis much more daunting. In contrast, the higher oil content and fewer lipid-related genes in the small and diploid genome of sesame make it an invaluable potential model plant for studying oil biosynthesis. The de novo assembled genome, a set of 12 transcriptomes and 29 resequenced accessions provide a large resource for exploring the mechanisms underlying different oil content between sesame and soybean, as well as among sesame accessions. The extensive expansion and high diversity of LTP1 genes, and key genes differentially expressed in downstream of the TAG biosynthesis pathway should aid future genetic studies in sesame. Undoubtedly, future studies on sesame will help to improve the quantity and quality of edible oil crops in order to fight food and nutrition crises.
Materials and methods
DNA and RNA isolation
The genotype used for whole genome de novo sequencing was ‘Zhongzhi No. 13’, an elite sesame cultivar that has been introduced to most of the major sesame planting areas over the past 10 years. Genomic DNA was extracted from leaves with a standard CTAB (Cetyl trimethylammonium bromide) extraction method . The materials used for RNA-Seq to analyze lipid and sesamin synthesis were three sesame accessions with different lipid and sesamin content (Table S1 in Additional file 1). The seeds of 10, 20, 25 and 30 DPA plants were sampled for RNA-Seq. The procedure described by Wei et al.  was used for RNA extraction and sequencing (or see Additional file 1).
Whole genome shotgun sequencing and assembly
We carried out whole genome shotgun sequencing with the Illumina Hiseq 2000 platform. Eight paired-end sequencing libraries with insert sizes of approximately 180 bp, 500 bp, 800 bp, 2 kb, 5 kb, 10 kb and 20 kb were constructed, which generated a total data amount of 99.54 Gb. To reduce the effect of sequencing error on assembly, we applied a series of stringent filtering steps on read generation (see Supplementary Note in Additional file 1). After the above quality-control and filtering steps, 54.46 Gb of clean data, approximately 150-fold coverage of the predicted genome size, remained (Table S2 in Additional file 1; Data S1 in Additional file 2). The quality and quantity of the filtered data were checked by the distributions of the clean reads from every library (Figure S1 in Additional file 1). For all of the 37.63 Gb of clean data from short-insert size libraries, a custom program based on the k-mer frequency methodology was used to trim reads and correct bases . Next, all of the remaining data were used for de novo genome assembly. We carried out the whole genome assembly using SOAPdenovo [30, 52].
First, we split the reads from the short-insert size libraries into k-mers (k = 71) and constructed a de Bruijn graph. We then simplified the graph referring to the parameters, and lastly connected the k-mer path to produce the contig file.
All usable reads were realigned onto the contig sequences; the amount of shared paired end relationships between each pair of contigs and the rate of consistent and conflicting paired-ends were calculated to construct the scaffolds step by step, from short-insertion-size paired ends to long-insertion-size paired ends, and finally, scaffolds.
We used the tool GapCloser  to close the gaps inside the constructed scaffolds, which were mainly composed of repeats masked before scaffold construction. We used the paired-end information to retrieve the read pairs that had one end mapped to the unique contig and the other located in the gap region. Then, we preformed local assembly for these collected reads. Finally, about 274 Mb of the sesame genome was assembled, 98.8% of which is non-gapped sequences (Additional file 1).
Estimation of genome size by flow cytometry
Flow cytometry was used to determine the DNA content of sesame . Sesame samples and reference material were analyzed on an EPICS Elite ESP cytometer (Beckman-Coulter, Hialeah, FL, USA) with an air-cooled argon laser (Uniphase) at 488 nm using 20 mW. Salmon erythrocytes (2.16 pg/1C) were used as internal biological reference materials. Nuclear DNA content (in picograms) of sesame samples was estimated according to the following equation: 1C nuclear DNA content = (1C reference in picograms × Peak mean of sesame)/(Peak mean of reference). The number of base pairs per haploid genome was calculated based on the equivalent of 1 pg DNA = 978 Mb . As a result, the C-value of sesame was estimated to be 0.34 pg/1C, and its genome size was estimated to be approximately 337 Mb (Figure S3 in Additional file 1).
Anchoring of genome assembly to sesame genetic map
We used a combination method of specific length amplified fragment sequencing and experiment marker analysis to construct a new genetic map using 107 F2 lines derived from the Zhongzhi No.13/ZZM2289 population. In total, 2,719 SNPs, 97 insertions and deletions (indels) and 2,282 SSR markers were developed and screened against the population. After filtering the markers with low PCR quality, those having no polymorphism and those showing significantly distorted segregation in the population, the retained 45 indels, 259 SNPs and 124 SSR markers were used to construct the genetic map using JoinMap 3 software (Kyazma BV, Wageningen, Netherlands). Finally, we successfully constructed a genetic map that spans 1790.08 cM and has 406 markers, including 39 indels, 251 SNPs and 116 SSR markers. Based on the genetic map, 150 large scaffolds were anchored onto 16 pseudomolecules (see details in the Supplementary Note 3 in Additional file 1).
Gene structure prediction and function annotation
To predict genes in the assembled genome, we used both homology-based and de novo methods. For the homology-based prediction, A. thaliana, grapevine, castor, and potato proteins were mapped onto the assembled genome using Genewise  to define gene models. For de novo prediction, Augustus  and GlimmerHMM were employed using appropriate parameters. Data from these complementary analyses were merged to produce a non-redundant reference gene set using GLEAN . In addition, RNA-Seq data from multiple tissues (young roots, leaves, flowers, developing seeds, and shoot tips) from our previous study  were also incorporated to aid in gene annotation. RNA-Seq data were mapped to the assembled genome using TopHat , and transcriptome-based gene structures were obtained by cufflinks . Then, we compared this gene set with the previous one to get the final non-redundant gene set of sesame (Tables S8 to S10 in Additional file 1). The non-coding gene predictions and gene function annotations were conducted as described in Supplementary Note 3 and Table S11 in Additional file 1.
We identified repeat content in the sesame genome using a combination of de novo and homology-based approaches (Supplementary Note and Tables S12 and S13 in Additional file 1). Full-length LTR retrotransposons were identified by LTR_STRUC  and classified as Gypsy, Copia and other types of transposons using the program RepeatClassifer implemented in the RepeatModeler package . Then the insertion time of LTR retrotransposons was dated according to the method described by JessyLabbé  (Supplementary Note and Figure S8 in Additional file 1).
Gene clustering was conducted with OrthoMCL  by setting the main inflation value to 1.5 and other parameters as default. PHYML  was selected to reconstruct the phylogenetic tree based on the HKY85 model . The program MCMCTree of the PAML package  was used to estimate species divergence time. Mcscan  was used to construct chromosome collinearity. Detailed descriptions about the identification of recent WGD events and two subgenomes are provided in Supplementary Note 4 in Additional file 1.
Analysis of resistance genes in sesame
HMMER V3.0  was used to screen the predicted sesame proteome against the raw hidden Markov model corresponding to the Pfam NBS (NB-ARC), and further build a sesame-specific NBS hidden Markov model for screening. The TIR and LRR domains were identified using local Pfam_Scan (-E 0.01 --domE 0.01). MARCOIL  with a threshold probability of 90 and the program paircoil2  with a P-score cutoff of 0.025 were used as the settings for the CC motif identification.
The absence of NBS genes with a TIR domain in the sesame genome was further validated by checking the gene-masked assembly and the unassembled reads. For the masked assembly, we found nine NB-ARC fragments (>300 bp), but no TIR hit was obtained. Among all the unmapped reads, only 19 showed homology to the TIR domain, but all the reads together covered less than half of the TIR region. Considering the above results, NBS genes with a TIR domain were absent from sesame (for detailed methods see Supplementary Note 5 in Additional file 1).
Analysis of important characteristics in the genome
The homologous lipid genes in sesame and other crops were identified by blastp (1e-5, identity >30%) based on the database of acyl-Lipid metabolism in A. thaliana (for detailed methods see Supplementary Note 7 in Additional file 1).
Genome resequencing and SNP calling
For each accession, a paired-end sequencing library with insert sizes of 500 bp was constructed and then sequenced on the Hiseq 2000 platform. The raw reads were then subjected to a series of stringent filtering steps that had been used in de novo genome assembly (Supplementary Note 1.3 in Additional file 1). Finally, we generated a total of more than 120 Gb clean data with each sample at over 13-fold sequence depth (Data S11 in Additional file 2). The clean reads were mapped to the assembled sesame genome using BWA software . After mapping, SNPs were identified with read mapping quality ≥20 on the basis of the mpileup files generated by SAMtools  (Data S7 in Additional file 2). The SNPs extracted by the above process were first filtered by the sequencing depth: ≥30 and ≤581 using the vcfutils program in SAMtools. Then the raw SNP sites were further filtered using the following criteria: copy number ≤2 and a minimum of 5 bp apart with the exception of minor allele frequencies (≥0.05), whereby SNPs were retained when the distance between SNPs was less than 5 bp. Diversity parameters π and θ w were measured using a window of 10 kb with a step of 1 kb [43, 45].
Genomic data generated by the whole project are available at NCBI under accession number APMJ00000000 . WGS raw reads are deposited under the SRA study: SRA122008 . The raw RNA-Seq data are deposited under the SRA study: SRA122023 . Genome assembly, annotation and RNA-Seq data are also available at .
days post anthesis
insertion and deletion
long terminal repeat
million years ago
polymerase chain reaction
single nucleotide polymorphism
simple sequence repeat
whole genome duplication.
Troncoso-Ponce MA, Kilaru A, Cao X, Durrett TP, Fan J, Jensen JK, Thrower NA, Pauly M, Wilkerson C, Ohlrogge JB: Comparative deep transcriptional profiling of four developing oilseeds. Plant J. 2011, 68: 1014-1027.
Wei W, Zhang Y, Lv H, Li D, Wang L, Zhang X: Association analysis for quality traits in a diverse panel of Chinese sesame (Sesamum indicum L.) germplasm. J Integr Plant Biol. 2013, 58: 745-758.
Ke T, Mao H, Hui FL, Dong CH, Chai GH, Liu SY: Bioinformatics analysis and functional annotation of complete expressed sequence tag collection for oil crops. China J Bioinformatics. 2010, 8: 165-170.
Johnson LA, Suleiman TM, Lusas EW: Sesame protein: a review and prospectus. J Am Oil Chem Soc. 1979, 56: 463-468.
Miyake Y, Fukumoto S, Okada M, Sakaida K, Nakamura Y, Osawa T: Antioxidative catechol lignans converted from sesamin and sesaminol triglucoside by culturing with Aspergillus. J Agric Food Chem. 2005, 53: 22-27.
Hirata F, Fujita K, Ishikura Y, Hosoda K, Ishikawa T, Nakamura H: Hypocholesterolemic effect of sesame lignan in humans. Atherosclerosis. 1996, 122: 135-136.
Tsai CM, Chen PR, Chien KL, Su TC, Chang CJ, Liu TL, Cheng HC: Dietary sesame reduces serum cholesterol and enhances antioxidant capacity in hypercholesterolemia. Nutr Res. 2005, 25: 559-567.
Noguchi A, Fukui Y, Iuchi-Okada A, Kakutani S, Satake H, Iwashita T, Nakao M, Umezawa T, Ono E: Sequential glucosylation of a furofuran lignan, (+)-sesaminol, by Sesamum indicum UGT71A9 and UGT94D1 glucosyltransferases. Plant J. 2008, 54: 415-427.
Angiosperm Phylogeny Website. http://www.mobot.org/MOBOT/research/APweb/,
Wang L, Zhang Y, Qi X, Gao Y, Zhang X: Development and characterization of 59 polymorphic cDNA-SSR markers for the edible oil crop Sesamum indicum (Pedaliaceae). Am J Bot. 2012, 99: e394-e398.
Zhang H, Wei L, Miao H, Zhang T, Wang C: Development and validation of genic-SSR markers in sesame by RNA-seq. BMC Genomics. 2012, 13: 316-
Spandana B, Reddy VP, Prasanna GJ, Anuradha G, Sivaramakrishnan S: Development and characterization of microsatellite markers (SSR) in Sesamum (Sesamum indicum L.) species. Appl Biochem Biotechnol. 2012, 168: 1594-1607.
Uzun B, Lee D, Donini P, Cagirgan MI: Identification of a molecular marker linked to the closed capsule mutant trait in sesame using AFLP. Plant Breed. 2003, 122: 95-97.
Uzun B, Cagirgan MI: Identification of molecular markers linked to determinate growth habit in sesame. Euphytica. 2009, 166: 379-384.
Zhang H, Miao H, Wei L, Li C, Zhao R, Wang C: Genetic analysis and QTL mapping of seed coat color in sesame (Sesamum indicum L.). PLoS One. 2013, 8: e63898-
Suh MC, Kim MJ, Hur CG, Bae JM, Park YI, Chung CH, Kang CW, Ohlrogge JB: Comparative analysis of expressed sequence tags from Sesamum indicum and Arabidopsis thaliana developing seeds. Plant Mol Biol. 2003, 52: 1107-1123.
Wei W, Qi X, Wang L, Zhang Y, Hua W, Li D, Lv H, Zhang X: Characterization of the sesame (Sesamum indicum L.) global transcriptome using Illumina paired-end sequencing and development of EST-SSR markers. BMC Genomics. 2011, 12: 451-
Wang L, Zhang Y, Qi X, Li D, Wei W, Zhang X: Global gene expression responses to waterlogging in roots of sesame (Sesamum indicum L.). Acta Physiol Plant. 2012, 34: 2241-2249.
Yi DK, Kim KJ: Complete chloroplast genome sequences of important oilseed crop Sesamum indicum L. PLoS One. 2012, 7: e35872-
Ibarra-Laclette E, Lyons E, Hernandez-Guzman G, Perez-Torres CA, Carretero-Paulet L, Chang TH, Lan T, Welch AJ, Juarez MJ, Simpson J, Fernández-Cortés A, Arteaga-Vázquez M, Góngora-Castillo E, Acevedo-Hernández G, Schuster SC, Himmelbauer H, Minoche AE, Xu S, Lynch M, Oropeza-Aburto A, Cervantes-Pérez SA, de Jesús Ortega-Estrada M, Cervantes-Luevano JI, Michael TP, Mockler T, Bryant D, Herrera-Estrella A, Albert VA, Herrera-Estrella L: Architecture and evolution of a minute plant genome. Nature. 2013, 498: 94-98.
Zhang H, Miao H, Wang L, Qu L, Liu H, Wang Q, Yue M: Genome sequencing of the important oilseed crop Sesamum indicum L. Genome Biol. 2013, 14: 401-
Li Y, Hu Y, Bolund L, Wang J: State of the art de novo assembly of human genomes from massively parallel sequencing data. Hum Genomics. 2010, 4: 271-277.
Carlsson AS, Pham TD, Bui TM, Werlemark G, Bui TC, Merker A: A study of genetic diversity of sesame (Sesamum indicum L.) in Vietnam and Cambodia estimated by RAPD markers. Genet Resour Crop Evol. 2009, 56: 679-690.
Jaillon O, Aury JM, Noel B, Policriti A, Clepet C, Casagrande A, Choisne N, Aubourg S, Vitulo N, Jubin C, Vezzi A, Legeai F, Hugueney P, Dasilva C, Horner D, Mica E, Jublot D, Poulain J, Bruyère C, Billault A, Segurens B, Gouyvenoux M, Ugarte E, Cattonaro F, Anthouard V, Vico V, Del Fabbro C, Alaux M, Di Gaspero G, Dumas V, et al: The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla. Nature. 2007, 449: 463-467.
The Tomato Genome Consortium: The tomato genome sequence provides insights into fleshy fruit evolution. Nature. 2012, 485: 635-641.
Xu X, Pan S, Cheng S, Zhang B, Mu D, Ni P, Zhang G, Yang S, Li R, Wang J, Orjeda G, Guzman F, Torres M, Lozano R, Ponce O, Martinez D, De la Cruz G, Chakrabarti SK, Patil VU, Skryabin KG, Kuznetsov BB, Ravin NV, Kolganova TV, Beletsky AV, Mardanov AV, Di Genova A, Bolser DM, Martin DM, Li G, Yang Y, et al: Genome sequence and analysis of the tuber crop potato. Nature. 2011, 475: 189-195.
Du J, Tian Z, Hans CS, Laten HM, Cannon SB, Jackson SA, Shoemaker RC, Ma J: Evolutionary conservation, diversity and specificity of LTR-retrotransposons in flowering plants: insights from genome-wide analysis and multi-specific comparison. Plant J. 2010, 63: 584-598.
The Angiosperm Phylogeny G: An update of the Angiosperm Phylogeny Group classification for the orders and families of flowering plants: APG III. Bot J Linn Soc. 2009, 161: 105-121.
Wang X, Wang H, Wang J, Sun R, Wu J, Liu S, Bai Y, Mun JH, Bancroft I, Cheng F, Huang S, Li X, Hua W, Wang J, Wang X, Freeling M, Pires JC, Paterson AH, Chalhoub B, Wang B, Hayward A, Sharpe AG, Park BS, Weisshaar B, Liu B, Li B, Liu B, Tong C, Song C, Duran C, et al: The genome of the mesopolyploid crop species Brassica rapa. Nat Genet. 2011, 43: 1035-1039.
Meyers BC, Dickerman AW, Michelmore RW, Sivaramakrishnan S, Sobral BW, Young ND: Plant disease resistance genes encode members of an ancient and diverse protein family within the nucleotide-binding superfamily. Plant J. 1999, 20: 317-332.
Tarr DE, Alexander HM: TIR-NBS-LRR genes are rare in monocots: evidence from diverse monocot orders. BMC Res Notes. 2009, 2: 197-
Tian Y, Fan L, Thurau T, Jung C, Cai D: The absence of TIR-type resistance gene analogues in the sugar beet (Beta vulgaris L.) genome. J Mol Evol. 2004, 58: 40-53.
Yue JX, Meyers BC, Chen JQ, Tian D, Yang S: Tracing the origin and evolutionary history of plant nucleotide-binding site-leucine-rich repeat (NBS-LRR) genes. New Phytol. 2012, 193: 1049-1063.
Kader JC: Lipid-transfer proteins in plants. Annu Rev Plant Physiol Plant Mol Biol. 1996, 47: 627-654.
Thompson JE, Froese CD, Madey E, Smith MD, Hong Y: Lipid metabolism during plant senescence. Prog Lipid Res. 1998, 37: 119-141.
Feussner I, Kuhn H, Wasternack C: Lipoxygenase-dependent degradation of storage lipids. Trends Plant Sci. 2001, 6: 268-273.
Lu C, Xin Z, Ren Z, Miquel M, Browse J: An enzyme regulating triacylglycerol composition is encoded by the ROD1 gene of Arabidopsis. Proc Natl Acad Sci U S A. 2009, 106: 18837-18842.
Mhaske V, Beldjilali K, Ohlrogge J, Pollard M: Isolation and characterization of an Arabidopsis thaliana knockout line for phospholipid: diacylglycerol transacylase gene (At5g13640). Plant Physiol Biochem. 2005, 43: 413-417.
Cases S, Stone SJ, Zhou P, Yen E, Tow B, Lardizabal KD, Voelker T, Farese RV: Cloning of DGAT2, a second mammalian diacylglycerol acyltransferase, and related family members. J Biol Chem. 2001, 276: 38870-38876.
Dahlqvist A, Stahl U, Lenman M, Banas A, Lee M, Sandager L, Ronne H, Stymne S: Phospholipid: diacylglycerol acyltransferase: an enzyme that catalyzes the acyl-CoA-independent formation of triacylglycerol in yeast and plants. Proc Natl Acad Sci U S A. 2000, 97: 6487-6492.
Oelkers P, Cromley D, Padamsee M, Billheimer JT, Sturley SL: The DGA1 gene determines a second triglyceride synthetic pathway in yeast. J Biol Chem. 2002, 277: 8877-8881.
Xu X, Liu X, Ge S, Jensen JD, Hu F, Li X, Dong Y, Gutenkunst RN, Fang L, Huang L, Li J, He W, Zhang G, Zheng X, Zhang F, Li Y, Yu C, Kristiansen K, Zhang X, Wang J, Wright M, McCouch S, Nielsen R, Wang J, Wang W: Resequencing 50 accessions of cultivated and wild rice yields markers for identifying agronomically important genes. Nat Biotechnol. 2012, 30: 105-111.
Varshney RK, Song C, Saxena RK, Azam S, Yu S, Sharpe AG, Cannon S, Baek J, Rosen BD, Tar’an B, Millan T, Zhang X, Ramsay LD, Iwata A, Wang Y, Nelson W, Farmer AD, Gaur PM, Soderlund C, Penmetsa RV, Xu C, Bharti AK, He W, Winter P, Zhao S, Hane JK, Carrasquilla-Garcia N, Condie JA, Upadhyaya HD, Luo MC, et al: Draft genome sequence of chickpea (Cicer arietinum) provides a resource for trait improvement. Nat Biotechnol. 2013, 31: 240-246.
Guo S, Zhang J, Sun H, Salse J, Lucas WJ, Zhang H, Zheng Y, Mao L, Ren Y, Wang Z, Min J, Guo X, Murat F, Ham BK, Zhang Z, Gao S, Huang M, Xu Y, Zhong S, Bombarely A, Mueller LA, Zhao H, He H, Zhang Y, Zhang Z, Huang S, Tan T, Pang E, Lin K, Hu Q, et al: The draft genome of watermelon (Citrullus lanatus) and resequencing of 20 diverse accessions. Nat Genet. 2012, 45: 51-58.
Lam HM, Xu X, Liu X, Chen W, Yang G, Wong FL, Li MW, He W, Qin N, Wang B, Min J, Guo X, Murat F, Ham BK, Zhang Z, Gao S, Huang M, Xu Y, Zhong S, Bombarely A, Mueller LA, Zhao H, He H, Zhang Y, Zhang Z, Huang S, Tan T, Pang E, Lin K, Hu Q: Resequencing of 31 wild and cultivated soybean genomes identifies patterns of genetic diversity and selection. Nat Genet. 2010, 42: 1053-1059.
Zhang X, Li P, Wang X, Wang X: Studies on relationship among lignans, oil and protein content in sesame seed. Chin J Oil Crop Sci. 2005, 27: 88-90.
Kim HJ, Ono E, Morimoto K, Yamagaki T, Okazawa A, Kobayashi A, Satake H: Metabolic engineering of lignan biosynthesis in Forsythia cell culture. Plant Cell Physiol. 2009, 50: 2200-2209.
Greenbaum D, Colangelo C, Williams K, Gerstein M: Comparing protein abundance and mRNA expression levels on a genomic scale. Genome Biol. 2003, 4: 117-
Ono E, Nakai M, Fukui Y, Tomimori N, Fukuchi-Mizutani M, Saito M, Satake H, Tanaka T, Katsuta M, Umezawa T, Tanaka Y: Formation of two methylenedioxy bridges by a Sesamum CYP81Q protein yielding a furofuran lignan, (+)-sesamin. Proc Natl Acad Sci U S A. 2006, 103: 10116-10121.
Doyle JJ, Doyle JL: Isolation of plant DNA from fresh tissue. Focus. 1990, 12: 13-15.
Li R, Zhu H, Ruan J, Qian W, Fang X, Shi Z, Li Y, Li S, Shan G, Kristiansen K, Yang H, Wang J: De novo assembly of human genomes with massively parallel short read sequencing. Genome Res. 2010, 20: 265-272.
SOAPdenovo2: large-size Genome De-novo Assembler. http://sourceforge.net/projects/soapdenovo2/files/GapCloser/,
Dolezel J, Greilhuber J, Suda J: Estimation of nuclear DNA content in plants using flow cytometry. Nat Protocols. 2007, 2: 2233-2244.
Dolezel J, Bartos J, Voglmayr H, Greilhuber J: Nuclear DNA content and genome size of trout and human. Cytometry A. 2003, 51: 127-128. author reply 129
Birney E, Durbin R: Using GeneWise in the Drosophila annotation experiment. Genome Res. 2000, 10: 547-548.
Stanke M, Keller O, Gunduz I, Hayes A, Waack S, Morgenstern B: AUGUSTUS: ab initio prediction of alternative transcripts. Nucleic Acids Res. 2006, 34: W435-W439.
Trapnell C, Pachter L, Salzberg SL: TopHat: discovering splice junctions with RNA-Seq. Bioinformatics. 2009, 25: 1105-1111.
McCarthy EM, McDonald JF: LTR_STRUC: a novel search and identification program for LTR retrotransposons. Bioinformatics. 2003, 19: 362-367.
Labbe J, Murat C, Morin E, Tuskan GA, Le Tacon F, Martin F: Characterization of transposable elements in the ectomycorrhizal fungus Laccaria bicolor. PLoS One. 2012, 7: e40197-
Li L, Stoeckert CJ, Roos DS: OrthoMCL: identification of ortholog groups for eukaryotic genomes. Genome Res. 2003, 13: 2178-2189.
Guindon S, Dufayard JF, Lefort V, Anisimova M, Hordijk W, Gascuel O: New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst Biol. 2010, 59: 307-321.
Hasegawa M, Kishino H, Yano T: Dating of the human-ape splitting by a molecular clock of mitochondrial DNA. J Mol Evol. 1985, 22: 160-174.
Yang Z: PAML 4: phylogenetic analysis by maximum likelihood. Mol Biol Evol. 2007, 24: 1586-1591.
Finn RD, Clements J, Eddy SR: HMMER web server: interactive sequence similarity searching. Nucleic Acids Res. 2011, Web Server Issue39: W29-W37.
Delorenzi M, Speed T: An HMM model for coiled-coil domains and a comparison with PSSM-based predictions. Bioinformatics. 2002, 18: 617-625.
McDonnell AV, Jiang T, Keating AE, Berger B: Paircoil2: improved prediction of coiled coils from sequence. Bioinformatics. 2006, 22: 356-358.
Arabidopsis Acyl-lipid Metabolism. http://aralip.plantbiology.msu.edu,
Li H, Durbin R: Fast and accurate short read alignment with Burrows-Wheeler transform. Bioinformatics. 2009, 25: 1754-1760.
Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N, Marth G, Abecasis G, Durbin R: The Sequence Alignment/Map format and SAMtools. Bioinformatics. 2078–2079, 2009: 25-
Zheng LY, Guo XS, He B, Sun LJ, Peng Y, Dong SS, Liu TF, Jiang S, Ramachandran S, Liu CM, Jing HC: Genome-wide patterns of genetic variation in sweet and grain sorghum (Sorghum bicolor). Genome Biol. 2011, 12: R114-
Jiao Y, Zhao H, Ren L, Song W, Zeng B, Guo J, Wang B, Liu Z, Chen J, Li W, Zhang M, Xie S, Lai J: Genome-wide genetic changes during modern breeding of maize. Nat Genet. 2012, 44: 812-815.
Whole Genome Shotgun Sequencing Project of Sesamum indicum. http://www.ncbi.nlm.nih.gov/nuccore/APMJ00000000,
The Sequence Read Archive (SRA) of the Genome of Sesamum indicum. http://www.ncbi.nlm.nih.gov/sra/?term=SRA122008,
The Sequence Read Archive (SRA) of the Transcriptomes of Sesamum indicum. http://www.ncbi.nlm.nih.gov/sra/?term=SRA122023,
Sinbase: A Comprehensive Sesamum indicum Genomics Database. http://ocri-genomics.org/Sinbase,
The studies conducted by the Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences were supported by the National Basic Research Program of China (973 Program; no. 2011CB109304 and no. 2011CB109305), the National Natural Science Foundation of China (no. 31271766, no. 31101182 and no. 31201242), Central Public-interest Scientific Institution Basal Research Fund, and China Agriculture Research System (no. CARS - 15).
The authors declare that they have no competing interests.
XRZ, JW and SYL contributed to the design of the research. LHW, SY, CS, RKV and CBT participated in the genome analysis and wrote the manuscript. YZZ, YL and WH participated in the study design and constructed the genetic map. LHW, SY, SYL and RKV participated in co-ordination and finalization of the manuscript. CS, XDZ, YW, DL, FL and CYX participated in the genome and transcriptome analyses. XLH and SST participated in the resequencing and analysis. YXZ and DHL prepared materials and performed the experiments. JYY performed the database construction. JYW, XX, YRL and SMH participated in the statistical analysis. All authors read and approved the final manuscript.
Linhai Wang, Sheng Yu, Chaobo Tong, Yingzhong Zhao, Yan Liu contributed equally to this work.
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Wang, L., Yu, S., Tong, C. et al. Genome sequencing of the high oil crop sesame provides insight into oil biosynthesis. Genome Biol 15, R39 (2014). https://doi.org/10.1186/gb-2014-15-2-r39