Figure 8

The analysis of the FMR1 dataset by dCLIP. (a) An example of FMR1 binding site with stronger binding in the wild-type condition than the I304N condition. The total tag counts and T → C mutant tag counts are shown. Green bars indicate common binding regions, and blue bars indicate regions with stronger binding in the wild-type than the I304N condition. The peak heights are scaled proportionally to the total sequencing depths of the two samples. (b) The relative counts of T → C mutations on top of all ACTT/ACTG and TGGA motifs found within the 9,859 binding sites. The T → C mutation counts on the T bases in these motifs are divided by the total T → C counts in a 30 bp window as background distribution. Because the sequences surrounding these motifs vary and for each base outside these motifs only a fraction of the 9,859 binding sites have T base, they are all marked as N. The P- values shown are for testing the differences in the proportions of T → C counts on top of each motif out of the total T → C counts in the 30 bp window between the wild-type condition (pink line) and I304N condition (blue line). WT, wild-type.