The expressional differences of target genes found by only one method and not the other. The expression profiles of all genes constitute the background distribution. P-values were calculated by a one-sided Kolmogorov-Smirnov test comparing method-specific genes with the background distribution. The x- axis shows the cutoff, and the y- axis shows the percentage of genes that have differential expression greater than the cutoff (as compared to background). ko, knockout; wt, wild-type.